Restriction map for pF1KB4009
 (Linear) (Six-base) MAPSORT of: KIBB4009.seq  Check: 2457  from: 1  to: 2391



KIBB4009.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     309    1499    2391 
   Size:        309    1190     892

  Fragments arranged by size: 

                1190     892     309
 
AatII G_ACGT'C

Cuts at:      0    1331    2391 
   Size:       1331    1060
 
AccI GT'mk_AC

Cuts at:      0    2063    2391 
   Size:       2063     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     346    1169    2391 
   Size:        181     165     823    1222

  Fragments arranged by size: 

                1222     823     181     165
 
AfeI AGC'GCT

Cuts at:      0    1422    2391 
   Size:       1422     969
 
AflII C'TTAA_G

Cuts at:      0     255    2391 
   Size:        255    2136
 
AflIII A'CryG_T

Cuts at:      0    1202    2391 
   Size:       1202    1189
 
AgeI A'CCGG_T

Cuts at:      0    2304    2391 
   Size:       2304      87
 
AleI CACnn'nnGTG

Cuts at:      0    1312    2391 
   Size:       1312    1079
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1485    2391 
   Size:       1485     906
 
ApaI G_GGCC'C

Cuts at:      0    2191    2391 
   Size:       2191     200
 
ApoI r'AATT_y

Cuts at:      0     721    2287    2391 
   Size:        721    1566     104

  Fragments arranged by size: 

                1566     721     104
 
AseI AT'TA_AT

Cuts at:      0     704     881    2391 
   Size:        704     177    1510

  Fragments arranged by size: 

                1510     704     177
 
AvaI C'yCGr_G

Cuts at:      0     191     574    1730    1785    1834    2391 
   Size:        191     383    1156      55      49     557

  Fragments arranged by size: 

                1156     557     383     191      55      49
 
BanI G'GyrC_C

Cuts at:      0    1441    1594    1725    2028    2178    2221    2391 
   Size:       1441     153     131     303     150      43     170

  Fragments arranged by size: 

                1441     303     170     153     150     131      43
 
BanII G_rGCy'C

Cuts at:      0      82     192     284     614     623    1835    2191    2391 
   Size:         82     110      92     330       9    1212     356     200

  Fragments arranged by size: 

                1212     356     330     200     110      92      82       9
            
 
BbeI G_GCGC'C

Cuts at:      0    1598    2391 
   Size:       1598     793
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1166    2084    2391 
   Size:       1166     918     307

  Fragments arranged by size: 

                1166     918     307
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1117    1274    2391 
   Size:       1117     157    1117

  Fragments arranged by size: 

                1117    1117     157
 
BclI T'GATC_A

Cuts at:      0     381    2391 
   Size:        381    2010
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1573    2391 
   Size:       1573     818
 
BlpI GC'TnA_GC

Cuts at:      0    1888    2391 
   Size:       1888     503
 
Bme1580I G_kGCm'C

Cuts at:      0    1730    2191    2391 
   Size:       1730     461     200

  Fragments arranged by size: 

                1730     461     200
 
BmgBI CAC'GTC

Cuts at:      0    2214    2391 
   Size:       2214     177
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     251    2391 
   Size:        251    2140
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     960    2391 
   Size:        960    1431
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     914    2391 
   Size:        111     803    1477

  Fragments arranged by size: 

                1477     803     111
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1804    2391 
   Size:       1804     587
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     204    1078    1212    1326    1515    2391 
   Size:        204     874     134     114     189     876

  Fragments arranged by size: 

                 876     874     204     189     134     114
 
BsaAI yAC'GTr

Cuts at:      0    1008    2391 
   Size:       1008    1383
 
BsaHI Gr'CG_yC

Cuts at:      0    1328    1595    1658    2391 
   Size:       1328     267      63     733

  Fragments arranged by size: 

                1328     733     267      63
 
BsaWI w'CCGG_w

Cuts at:      0    1155    2304    2391 
   Size:       1155    1149      87

  Fragments arranged by size: 

                1155    1149      87
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     398     428    1594    1624    1981    2011    2391 
   Size:        398      30    1166      30     357      30     380

  Fragments arranged by size: 

                1166     398     380     357      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     292    2160    2391 
   Size:        292    1868     231

  Fragments arranged by size: 

                1868     292     231
 
BseYI C'CCAG_C

Cuts at:      0    2000    2391 
   Size:       2000     391
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1387    1471    1698    2160    2391 
   Size:       1387      84     227     462     231

  Fragments arranged by size: 

                1387     462     231     227      84
 
BsiEI CG_ry'CG

Cuts at:      0    1739    2391 
   Size:       1739     652
 
BsiHKAI G_wGCw'C

Cuts at:      0     284     614     623     915    1240    1304    1982    2117 
   Size:        284     330       9     292     325      64     678     135

Cuts at:   2117    2391 
   Size:        274

  Fragments arranged by size: 

                 678     330     325     292     284     274     135      64
                   9
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     192     284     614     623     915    1240    1304 
   Size:         82     110      92     330       9     292     325      64

Cuts at:   1304    1730    1835    1982    2117    2191    2391 
   Size:        426     105     147     135      74     200

  Fragments arranged by size: 

                 426     330     325     292     200     147     135     110
                 105      92      82      74      64       9
 
BspEI T'CCGG_A

Cuts at:      0    1155    2391 
   Size:       1155    1236
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     309    1493    1499    2018    2391 
   Size:        309    1184       6     519     373

  Fragments arranged by size: 

                1184     519     373     309       6
 
BsrBI CCG'CTC

Cuts at:      0     122    2016    2219    2391 
   Size:        122    1894     203     172

  Fragments arranged by size: 

                1894     203     172     122
 
BsrFI r'CCGG_y

Cuts at:      0    2031    2304    2391 
   Size:       2031     273      87

  Fragments arranged by size: 

                2031     273      87
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      42    2391 
   Size:         42    2349
 
BstBI TT'CG_AA

Cuts at:      0    2367    2391 
   Size:       2367      24
 
BstEII G'GTnAC_C

Cuts at:      0    1755    2391 
   Size:       1755     636
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1767    2391 
   Size:       1767     624
 
Bsu36I CC'TnA_GG

Cuts at:      0    1546    2021    2183    2391 
   Size:       1546     475     162     208

  Fragments arranged by size: 

                1546     475     208     162
 
BtgI C'CryG_G

Cuts at:      0    1088    1605    1826    2391 
   Size:       1088     517     221     565

  Fragments arranged by size: 

                1088     565     517     221
 
BtsI GCAGTG_nn'

Cuts at:      0    1419    2391 
   Size:       1419     972
 
EaeI y'GGCC_r

Cuts at:      0    1530    1736    1904    1958    2391 
   Size:       1530     206     168      54     433

  Fragments arranged by size: 

                1530     433     206     168      54
 
EagI C'GGCC_G

Cuts at:      0    1736    2391 
   Size:       1736     655
 
EarI CTCTTCn'nnn_

Cuts at:      0     602    2104    2110    2391 
   Size:        602    1502       6     281

  Fragments arranged by size: 

                1502     602     281       6
 
EcoICRI GAG'CTC

Cuts at:      0     282     612     621    2391 
   Size:        282     330       9    1770

  Fragments arranged by size: 

                1770     330     282       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     181     346     914    1169    2391 
   Size:        111      70     165     568     255    1222

  Fragments arranged by size: 

                1222     568     255     165     111      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     681    2391 
   Size:        681    1710
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     653    1482    1566    2187    2188    2228    2391 
   Size:        150     503     829      84     621       1      40     163

  Fragments arranged by size: 

                 829     621     503     163     150      84      40       1
            
 
EcoRI G'AATT_C

Cuts at:      0    2287    2391 
   Size:       2287     104
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      61    2208    2391 
   Size:         61    2147     183

  Fragments arranged by size: 

                2147     183      61
 
FspI TGC'GCA

Cuts at:      0    1873    2391 
   Size:       1873     518
 
FspAI rTGC'GCAy

Cuts at:      0    1873    2391 
   Size:       1873     518
 
HaeII r_GCGC'y

Cuts at:      0     598    1424    1598    1636    2391 
   Size:        598     826     174      38     755

  Fragments arranged by size: 

                 826     755     598     174      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     637     669     793     825    1105    1137 
   Size:          6      32     599      32     124      32     280      32

Cuts at:   1137    1420    1452    1594    1626    1768    1800    1981    2013 
   Size:        283      32     142      32     142      32     181      32

Cuts at:   2013    2391 
   Size:        378

  Fragments arranged by size: 

                 599     378     283     280     181     142     142     124
                  32      32      32      32      32      32      32      32
                   6
 
HincII GTy'rAC

Cuts at:      0    1458    2391 
   Size:       1458     933
 
KasI G'GCGC_C

Cuts at:      0    1594    2391 
   Size:       1594     797
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1187    2391 
   Size:       1187    1204
 
MslI CAynn'nnrTG

Cuts at:      0     541    1312    2391 
   Size:        541     771    1079

  Fragments arranged by size: 

                1079     771     541
 
MspA1I CmG'CkG

Cuts at:      0     228    1607    1692    1815    2391 
   Size:        228    1379      85     123     576

  Fragments arranged by size: 

                1379     576     228     123      85
 
NarI GG'CG_CC

Cuts at:      0    1595    2391 
   Size:       1595     796
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1206    2391 
   Size:       1206    1185
 
PfoI T'CCnGG_A

Cuts at:      0     614    2391 
   Size:        614    1777
 
PmlI CAC'GTG

Cuts at:      0    1008    2391 
   Size:       1008    1383
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     653    1482    2228    2391 
   Size:        150     503     829     746     163

  Fragments arranged by size: 

                 829     746     503     163     150
 
PspOMI G'GGCC_C

Cuts at:      0    2187    2391 
   Size:       2187     204
 
PstI C_TGCA'G

Cuts at:      0    1574    1697    2391 
   Size:       1574     123     694

  Fragments arranged by size: 

                1574     694     123
 
PvuII CAG'CTG

Cuts at:      0     228    1815    2391 
   Size:        228    1587     576

  Fragments arranged by size: 

                1587     576     228
 
SacI G_AGCT'C

Cuts at:      0     284     614     623    2391 
   Size:        284     330       9    1768

  Fragments arranged by size: 

                1768     330     284       9
 
SacII CC_GC'GG

Cuts at:      0    1608    2391 
   Size:       1608     783
 
SanDI GG'GwC_CC

Cuts at:      0     653    2228    2391 
   Size:        653    1575     163

  Fragments arranged by size: 

                1575     653     163
 
SapI GCTCTTCn'nnn_

Cuts at:      0     602    2104    2391 
   Size:        602    1502     287

  Fragments arranged by size: 

                1502     602     287
 
SbfI CC_TGCA'GG

Cuts at:      0    1574    2391 
   Size:       1574     817
 
SexAI A'CCwGG_T

Cuts at:      0    1751    2391 
   Size:       1751     640
 
SfcI C'TryA_G

Cuts at:      0     992    1424    1570    1693    2391 
   Size:        992     432     146     123     698

  Fragments arranged by size: 

                 992     698     432     146     123
 
SfoI GGC'GCC

Cuts at:      0    1596    2391 
   Size:       1596     795
 
SmaI CCC'GGG

Cuts at:      0    1732    1836    2391 
   Size:       1732     104     555

  Fragments arranged by size: 

                1732     555     104
 
SmlI C'TyrA_G

Cuts at:      0     255    1819    2391 
   Size:        255    1564     572

  Fragments arranged by size: 

                1564     572     255
 
SrfI GCCC'GGGC

Cuts at:      0    1836    2391 
   Size:       1836     555
 
StyI C'CwwG_G

Cuts at:      0       9     677     768    1760    2391 
   Size:          9     668      91     992     631

  Fragments arranged by size: 

                 992     668     631      91       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1369    2391 
   Size:       1369    1022
 
TatI w'GTAC_w

Cuts at:      0    1247    2391 
   Size:       1247    1144
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2331    2363    2391 
   Size:       2331      32      28

  Fragments arranged by size: 

                2331      32      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1914    2391 
   Size:       1914     477
 
XmaI C'CCGG_G

Cuts at:      0    1730    1834    2391 
   Size:       1730     104     557

  Fragments arranged by size: 

                1730     557     104
 
XmnI GAAnn'nnTTC

Cuts at:      0     860    2391 
   Size:        860    1531
 
ZraI GAC'GTC

Cuts at:      0    1329    2391 
   Size:       1329    1062
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI    AflII   AflIII     AgeI
     AleI    AlwNI     ApaI     ApoI     AseI     AvaI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspMI    BsrBI    BsrFI   BstAPI    BstBI   BstEII    BstXI   Bsu36I
     BtgI     BtsI     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     KasI     MmeI     MslI   MspA1I     NarI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
     SbfI    SexAI     SfcI     SfoI     SmaI     SmlI     SrfI     StyI
    TaqII     TatI     TstI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AhdI     AlfI     AloI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BfrBI    BglII     BmtI
   Bpu10I    BsaBI    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI
   BssHII    BssSI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI    EcoRV     FseI  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI
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