Restriction map for pF1KB3988
##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        AscI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        Ksp632I     KspI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI           94  AAATTT               [GA]^AATT[CT]
AflII         109  CTTAAG               C^TTAAG
Alw44I        218  GTGCAC               G^TGCAC
AlwNI          20  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         218  GTGCAC               G^TGCAC
ApoI           94  AAATTT               [GA]^AATT[CT]
AseI          354  ATTAAT               AT^TAAT
AsnI          354  ATTAAT               AT^TAAT
BanII         329  GAGCTC               G[GA]GC[CT]^C
BbvI          471  GCTGC                GCAGC(8/12)
BciVI         185  GTATCC               GTATCC(6/5)
BfaI          349  CTAG                 C^TAG
BfrI          109  CTTAAG               C^TTAAG
BpmI          332  CTCCAG               CTGGAG(16/14)
BsaJI         321  CCTTGG               C^C[ACGT][ACGT]GG
BsmI          512  GCATTC               GAATGC(1/-1)
BsrDI         307  CATTGC               GCAATG(2/0)
BssKI         286  CCTGG                ^CC[ACGT]GG
BstF5I        227  CATCC                GGATG(2/0)
BstNI         286  CCTGG                CC^[AT]GG
EcoRII        286  CCTGG                ^CC[AT]GG
EcoT14I       321  CCTTGG               C^C[AT][AT]GG
FokI          227  CATCC                GGATG(9/13)
HinfI         484  GATTC                G^A[ACGT]TC
MaeI          349  CTAG                 C^TAG
MnlI          103  GAGG                 CCTC(7/6)
MvaI          286  CCTGG                CC^[AT]GG
MwoI          311  GCGTTGCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshBI         354  ATTAAT               AT^TAAT
SacI          329  GAGCTC               GAGCT^C
ScaI          336  AGTACT               AGT^ACT
ScrFI         286  CCTGG                CC^[ACGT]GG
SexAI         285  ACCTGGT              A^CC[AT]GGT
SfaNI         382  GATGC                GCATC(5/9)
SmlI          109  CTTAAG               C^T[CT][GA]AG
StuI          318  AGGCCT               AGG^CCT
StyI          321  CCTTGG               C^C[AT][AT]GG
TaqI            6  TCGA                 T^CGA
TfiI          484  GATTC                G^A[AT]TC
TseI          471  GCTGC                G^C[AT]GC
Tsp45I         34  GTCAC                ^GT[GC]AC
TthHB8I         6  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          337  GTAC                 GT^AC
              388  GTAC                
AspHI         218  GTGCAC               G[AT]GC[AT]^C
              329  GAGCTC              
BmyI          218  GTGCAC               G[AGT]GC[ACT]^C
              329  GAGCTC              
BsiHKAI       218  GTGCAC               G[AT]GC[AT]^C
              329  GAGCTC              
Bsp1286I      218  GTGCAC               G[AGT]GC[ACT]^C
              329  GAGCTC              
BsrI          248  ACTGG                ACTGG(1/-1)
              334  CCAGT               
Cac8I         316  GCAGGC               GC[ACGT]^[ACGT]GC
              428  GCAAGC              
HaeIII        203  GGCC                 GG^CC
              319  GGCC                
MaeIII         34  GTCAC                ^GT[ACGT]AC
              124  GTTAC               
MboII         280  GAAGA                GAAGA(8/7)
              379  GAAGA               
NlaIII         48  CATG                 CATG^
              190  CATG                
RsaI          337  GTAC                 GT^AC
              388  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AciI        AluI        Fnu4HI      ItaI        MseI        
Tru9I       Tsp509I     
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