Restriction map for pF1KB3903
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AscI        Asp718I     AspEI       AviII       
AvrII       BbrPI       BbsI        BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsiWI       BsmBI       BsmI        BspDI       BspLU11I    
BsrFI       BssSI       Bst1107I    BstAPI      BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      Eam1105I    EcoRI       EcoT22I     FseI        
FspI        HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RsrII       SapI        ScaI        SexAI       SfiI        
SgrAI       SnaBI       SphI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   75 enzymes) #
###################################

AccI         1449  GTCGAC               GT^[AC][GT]AC
AccIII       2220  TCCGGA               T^CCGGA
AcsI          817  AAATTT               [GA]^AATT[CT]
AcyI         2282  GGCGCC               G[GA]^CG[CT]C
AflIII        431  ACGTGT               A^C[GA][CT]GT
Alw44I       1534  GTGCAC               G^TGCAC
AlwNI        1382  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1506  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       525  AGCGCT               AGC^GCT
ApaLI        1534  GTGCAC               G^TGCAC
ApoI          817  AAATTT               [GA]^AATT[CT]
AspI          838  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI            2  TGGCCA               TGG^CCA
BanI         2282  GGCGCC               G^G[CT][GA]CC
BbeI         2282  GGCGCC               GGCGC^C
BcgI         1438  GCAATTACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1736  TGATCA               T^GATCA
BglI          211  GCCAAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         430  TACGTG               [CT]AC^GT[GA]
BsaHI        2282  GGCGCC               G[GA]^CG[CT]C
BsaI         2058  GGTCTC               GGTCTC(1/5)
BseAI        2220  TCCGGA               T^CCGGA
BsgI          735  CTGCAC               GTGCAG(16/14)
Bsp1407I     1487  TGTACA               T^GTACA
BspEI        2220  TCCGGA               T^CCGGA
BspHI        2069  TCATGA               T^CATGA
BsrDI        1686  CATTGC               GCAATG(2/0)
BsrGI        1487  TGTACA               T^GTACA
BssHII        220  GCGCGC               G^CGCGC
BstBI         605  TTCGAA               TT^CGAA
BstEII        393  GGTCACC              G^GT[ACGT]ACC
BstPI         393  GGTCACC              G^GT[ACGT]ACC
BstXI        1664  CCACTTATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1506  CCTCAGG              CC^T[ACGT]AGG
DrdI          712  GACTCAGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           58  CGGCCG               C^GGCCG
EclXI          58  CGGCCG               C^GGCCG
Eco47III      525  AGCGCT               AGC^GCT
Eco52I         58  CGGCCG               C^GGCCG
Eco81I       1506  CCTCAGG              CC^T[ACGT]AGG
EcoNI         345  CCTGCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       393  GGTCACC              G^GT[ACGT]ACC
EcoRV         688  GATATC               GAT^ATC
FbaI         1736  TGATCA               T^GATCA
HgaI          938  GACGC                GACGC(5/10)
Hin1I        2282  GGCGCC               G[GA]^CG[CT]C
HincII       1449  GTCGAC               GT[CT]^[GA]AC
HindII       1449  GTCGAC               GT[CT]^[GA]AC
KasI         2282  GGCGCC               G^GCGCC
MluNI           2  TGGCCA               TGG^CCA
MroI         2220  TCCGGA               T^CCGGA
MscI            2  TGGCCA               TGG^CCA
MslI         1665  CACTTATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         2282  GGCGCC               GG^CGCC
NspV          605  TTCGAA               TT^CGAA
PflFI         838  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1809  CCAGATCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2069  TCATGA               T^CATGA
SalI         1449  GTCGAC               G^TCGAC
SbfI          183  CCTGCAGG             CCTGCA^GG
SfoI         2282  GGCGCC               GGC^GCC
SfuI          605  TTCGAA               TT^CGAA
SmaI         2256  CCCGGG               CCC^GGG
SmlI         1171  CTTGAG               C^T[CT][GA]AG
SpeI         1027  ACTAGT               A^CTAGT
Sse8387I      183  CCTGCAGG             CCTGCA^GG
SspBI        1487  TGTACA               T^GTACA
SspI          478  AATATT               AAT^ATT
StuI         2134  AGGCCT               AGG^CCT
Tth111I       838  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1809  CCAGATCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2082  TCTAGA               T^CTAGA
XcmI         1809  CCAGATCTTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2256  CCCGGG               C^CCGGG
XmaI         2256  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AseI          481  ATTAAT               AT^TAAT
             1878  ATTAAT              
AsnI          481  ATTAAT               AT^TAAT
             1878  ATTAAT              
Asp700I       448  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              814  GAAAAATTTC          
BamHI         638  GGATCC               G^GATCC
              913  GGATCC              
BsiEI          58  CGGCCG               CG[GA][CT]^CG
              279  CGACCG              
BspMI         344  ACCTGC               ACCTGC(4/8)
              753  GCAGGT              
BsrBI         321  CCGCTC               CCGCTC(-3/-3)
             2266  GAGCGG              
EaeI            2  TGGCCA               [CT]^GGCC[GA]
               58  CGGCCG              
EarI         1166  CTCTTC               CTCTTC(1/4)
             1801  CTCTTC              
HaeII         525  AGCGCT               [GA]GCGC^[CT]
             2282  GGCGCC              
Ksp632I      1166  CTCTTC               CTCTTC(1/4)
             1801  CTCTTC              
KspI           55  CCGCGG               CCGC^GG
             1922  CCGCGG              
MaeII         338  ACGT                 A^CGT
              431  ACGT                
NcoI         1481  CCATGG               C^CATGG
             1518  CCATGG              
NspI          802  GCATGT               [GA]CATG^[CT]
             1979  ACATGC              
PshBI         481  ATTAAT               AT^TAAT
             1878  ATTAAT              
PvuII         896  CAGCTG               CAG^CTG
             1727  CAGCTG              
SacI          313  GAGCTC               GAGCT^C
             1390  GAGCTC              
SacII          55  CCGCGG               CCGC^GG
             1922  CCGCGG              
XmnI          448  GAACCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              814  GAAAAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
ApaI        AspHI       AvaI        AvaII       BanII       
BbvCI       BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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