Restriction map for pF1KB3844
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoRI       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SpeI        SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tth111I     XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

Alw44I       1729  GTGCAC               G^TGCAC
ApaI         1074  GGGCCC               GGGCC^C
ApaLI        1729  GTGCAC               G^TGCAC
BbsI         1690  GTCTTC               GAAGAC(2/6)
BpuAI        1690  GTCTTC               GAAGAC(2/6)
BsmBI        1138  GAGACG               CGTCTC(1/5)
DpnII         276  GATC                 ^GATC
EagI         1040  CGGCCG               C^GGCCG
EclXI        1040  CGGCCG               C^GGCCG
Eco52I       1040  CGGCCG               C^GGCCG
EcoRV         232  GATATC               GAT^ATC
MboI          276  GATC                 ^GATC
NdeII         276  GATC                 ^GATC
NspI          645  GCATGC               [GA]CATG^[CT]
PflMI        1715  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         299  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         964  CAGCTG               CAG^CTG
SacI          754  GAGCTC               GAGCT^C
SapI          543  GCTCTTC              GCTCTTC(1/4)
Sau3AI        276  GATC                 ^GATC
SmaI         1754  CCCGGG               CCC^GGG
SnaBI        1546  TACGTA               TAC^GTA
SphI          645  GCATGC               GCATG^C
Tsp509I       772  AATT                 ^AATT
Van91I       1715  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1754  CCCGGG               C^CCGGG
XmaI         1754  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AspHI         754  GAGCTC               G[AT]GC[AT]^C
             1729  GTGCAC              
AvaI          579  CTCGGG               C^[CT]CG[GA]G
             1754  CCCGGG              
AviII         416  TGCGCA               TGC^GCA
              767  TGCGCA              
BbeI          674  GGCGCC               GGCGC^C
             1303  GGCGCC              
BbrPI        1324  CACGTG               CAC^GTG
             1359  CACGTG              
BglI         1162  GCCAATGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1333  GCCCTGACGGC         
BsaI         1444  GAGACC               GGTCTC(1/5)
             1531  GAGACC              
BsiEI         204  CGACCG               CG[GA][CT]^CG
             1040  CGGCCG              
BsiHKAI       754  GAGCTC               G[AT]GC[AT]^C
             1729  GTGCAC              
BsoBI         579  CTCGGG               C^[CT]CG[GA]G
             1754  CCCGGG              
BsrBI         378  CCGCTC               CCGCTC(-3/-3)
              889  GAGCGG              
BsrDI         423  CATTGC               GCAATG(2/0)
             1538  GCAATG              
BsrI           98  ACTGG                ACTGG(1/-1)
             1022  ACTGG               
BstEII        691  GGTAACC              G^GT[ACGT]ACC
             1521  GGTCACC             
BstPI         691  GGTAACC              G^GT[ACGT]ACC
             1521  GGTCACC             
BstXI        1115  CCAGCGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1307  CCAAGACCCTGG        
EarI          544  CTCTTC               CTCTTC(1/4)
             1696  CTCTTC              
EcoNI         132  CCTGACGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1935  CCTCTGCAAGG         
EcoO65I       691  GGTAACC              G^GT[ACGT]ACC
             1521  GGTCACC             
FspI          416  TGCGCA               TGC^GCA
              767  TGCGCA              
HgaI          244  GACGC                GACGC(5/10)
              511  GACGC               
HincII        316  GTCAAC               GT[CT]^[GA]AC
             1048  GTCAAC              
HindII        316  GTCAAC               GT[CT]^[GA]AC
             1048  GTCAAC              
KasI          674  GGCGCC               G^GCGCC
             1303  GGCGCC              
Ksp632I       544  CTCTTC               CTCTTC(1/4)
             1696  CTCTTC              
KspI          930  CCGCGG               CCGC^GG
             1842  CCGCGG              
NarI          674  GGCGCC               GG^CGCC
             1303  GGCGCC              
PmaCI        1324  CACGTG               CAC^GTG
             1359  CACGTG              
PmlI         1324  CACGTG               CAC^GTG
             1359  CACGTG              
PshAI         135  GACGGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              309  GACCAAGGTC          
SacII         930  CCGCGG               CCGC^GG
             1842  CCGCGG              
SbfI          399  CCTGCAGG             CCTGCA^GG
              828  CCTGCAGG            
SfoI          674  GGCGCC               GGC^GCC
             1303  GGCGCC              
Sse8387I      399  CCTGCAGG             CCTGCA^GG
              828  CCTGCAGG            
TaqI          146  TCGA                 T^CGA
              941  TCGA                
Tsp45I         52  GTCAC                ^GT[GC]AC
             1522  GTCAC               
TthHB8I       146  TCGA                 T^CGA
              941  TCGA                
XcmI           74  CCACGGCCACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              428  CCAGGGACTCGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AvaII       BalI        BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BssKI       BstAPI      BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboII       MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NlaIII      NlaIV       PleI        
PstI        RsaI        Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TseI        TspRI       
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