Restriction map for pF1KB3844
 (Linear) (Six-base) MAPSORT of: KIBB3844.seq  Check: 8603  from: 1  to: 2022



KIBB3844.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     166     407     821    2022 
   Size:        166     241     414    1201

  Fragments arranged by size: 

                1201     414     241     166
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     714    2022 
   Size:        714    1308
 
AleI CACnn'nnGTG

Cuts at:      0      64    1566    2022 
   Size:         64    1502     456

  Fragments arranged by size: 

                1502     456      64
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     469     854    2022 
   Size:        469     385    1168

  Fragments arranged by size: 

                1168     469     385
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      88     120    2022 
   Size:         88      32    1902

  Fragments arranged by size: 

                1902      88      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     969    1863    1937    2022 
   Size:        969     894      74      85

  Fragments arranged by size: 

                 969     894      85      74
 
ApaI G_GGCC'C

Cuts at:      0    1078    2022 
   Size:       1078     944
 
ApaLI G'TGCA_C

Cuts at:      0    1729    2022 
   Size:       1729     293
 
AvaI C'yCGr_G

Cuts at:      0     579    1754    2022 
   Size:        579    1175     268

  Fragments arranged by size: 

                1175     579     268
 
BanI G'GyrC_C

Cuts at:      0     674    1303    1966    2022 
   Size:        674     629     663      56

  Fragments arranged by size: 

                 674     663     629      56
 
BanII G_rGCy'C

Cuts at:      0     122     758    1078    1435    1496    2022 
   Size:        122     636     320     357      61     526

  Fragments arranged by size: 

                 636     526     357     320     122      61
 
BbeI G_GCGC'C

Cuts at:      0     678    1307    2022 
   Size:        678     629     715

  Fragments arranged by size: 

                 715     678     629
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1683    2022 
   Size:       1683     339
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1168    1339    2022 
   Size:       1168     171     683

  Fragments arranged by size: 

                1168     683     171
 
Bme1580I G_kGCm'C

Cuts at:      0    1078    1733    2022 
   Size:       1078     655     289

  Fragments arranged by size: 

                1078     655     289
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1032    2022 
   Size:       1032     990
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     619     907    1510    2022 
   Size:        478     141     288     603     512

  Fragments arranged by size: 

                 603     512     478     288     141
 
Bpu10I CC'TnA_GC

Cuts at:      0     550     565     973    2022 
   Size:        550      15     408    1049

  Fragments arranged by size: 

                1049     550     408      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     520    1219    2022 
   Size:        289     231     699     803

  Fragments arranged by size: 

                 803     699     289     231
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1438    1525    2022 
   Size:       1438      87     497

  Fragments arranged by size: 

                1438     497      87
 
BsaAI yAC'GTr

Cuts at:      0    1326    1361    1548    1728    1899    2022 
   Size:       1326      35     187     180     171     123

  Fragments arranged by size: 

                1326     187     180     171     123      35
 
BsaHI Gr'CG_yC

Cuts at:      0     245     512     675    1304    2022 
   Size:        245     267     163     629     718

  Fragments arranged by size: 

                 718     629     267     245     163
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     613     643    1483    1513    2022 
   Size:        613      30     840      30     509

  Fragments arranged by size: 

                 840     613     509      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     520     616    1374    1906    2022 
   Size:        520      96     758     532     116

  Fragments arranged by size: 

                 758     532     520     116      96
 
BseYI C'CCAG_C

Cuts at:      0     802     962    1422    2022 
   Size:        802     160     460     600

  Fragments arranged by size: 

                 802     600     460     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     379     451    1474    2022 
   Size:        343      36      72    1023     548

  Fragments arranged by size: 

                1023     548     343      72      36
 
BsiEI CG_ry'CG

Cuts at:      0     207    1043    2022 
   Size:        207     836     979

  Fragments arranged by size: 

                 979     836     207
 
BsiHKAI G_wGCw'C

Cuts at:      0     758    1733    2022 
   Size:        758     975     289

  Fragments arranged by size: 

                 975     758     289
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1132    2022 
   Size:       1132     890
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     758    1078    1435    1496    1733    2022 
   Size:        122     636     320     357      61     237     289

  Fragments arranged by size: 

                 636     357     320     289     237     122      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      98     166     269     315     380     407     704     806 
   Size:         98      68     103      46      65      27     297     102

Cuts at:    806     821     836    1484    2022 
   Size:         15      15     648     538

  Fragments arranged by size: 

                 648     538     297     103     102      98      68      65
                  46      27      15      15
 
BsrBI CCG'CTC

Cuts at:      0     380     891    2022 
   Size:        380     511    1131

  Fragments arranged by size: 

                1131     511     380
 
BsrDI GCAATG_nn'

Cuts at:      0     422    1545    2022 
   Size:        422    1123     477

  Fragments arranged by size: 

                1123     477     422
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     158    1863    1937    2022 
   Size:        158    1705      74      85

  Fragments arranged by size: 

                1705     158      85      74
 
BstEII G'GTnAC_C

Cuts at:      0     691    1521    2022 
   Size:        691     830     501

  Fragments arranged by size: 

                 830     691     501
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1122    1314    2022 
   Size:       1122     192     708

  Fragments arranged by size: 

                1122     708     192
 
BtgI C'CryG_G

Cuts at:      0      74      83     930    1043    1181    1286    1292    1427 
   Size:         74       9     847     113     138     105       6     135

Cuts at:   1427    1526    1640    1842    2022 
   Size:         99     114     202     180

  Fragments arranged by size: 

                 847     202     180     138     135     114     113     105
                  99      74       9       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     108     699    2022 
   Size:        108     591    1323

  Fragments arranged by size: 

                1323     591     108
 
BtsI GCAGTG_nn'

Cuts at:      0      23      41    1685    2022 
   Size:         23      18    1644     337

  Fragments arranged by size: 

                1644     337      23      18
 
EaeI y'GGCC_r

Cuts at:      0      56      77     701     779     869    1040    1097    1160 
   Size:         56      21     624      78      90     171      57      63

Cuts at:   1160    1184    1289    1316    2022 
   Size:         24     105      27     706

  Fragments arranged by size: 

                 706     624     171     105      90      78      63      57
                  56      27      24      21
 
EagI C'GGCC_G

Cuts at:      0    1040    2022 
   Size:       1040     982
 
EarI CTCTTCn'nnn_

Cuts at:      0     550    1702    2022 
   Size:        550    1152     320

  Fragments arranged by size: 

                1152     550     320
 
EcoICRI GAG'CTC

Cuts at:      0     756    2022 
   Size:        756    1266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     619     714     907    1510    2022 
   Size:        478     141      95     193     603     512

  Fragments arranged by size: 

                 603     512     478     193     141      95
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     136    1939    2022 
   Size:        136    1803      83

  Fragments arranged by size: 

                1803     136      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     300     657    1059    1074    1075    1332    1763    2022 
   Size:        300     357     402      15       1     257     431     259

  Fragments arranged by size: 

                 431     402     357     300     259     257      15       1
            
 
EcoRV GAT'ATC

Cuts at:      0     234    2022 
   Size:        234    1788
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     561    2022 
   Size:        561    1461
 
FspI TGC'GCA

Cuts at:      0     418     769    2022 
   Size:        418     351    1253

  Fragments arranged by size: 

                1253     418     351
 
FspAI rTGC'GCAy

Cuts at:      0     418    2022 
   Size:        418    1604
 
HaeII r_GCGC'y

Cuts at:      0     678     809    1307    2022 
   Size:        678     131     498     715

  Fragments arranged by size: 

                 715     678     498     131
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     466     498    1483    1515    2022 
   Size:        466      32     985      32     507

  Fragments arranged by size: 

                 985     507     466      32      32
 
HincII GTy'rAC

Cuts at:      0     318    1050    2022 
   Size:        318     732     972

  Fragments arranged by size: 

                 972     732     318
 
KasI G'GCGC_C

Cuts at:      0     674    1303    2022 
   Size:        674     629     719

  Fragments arranged by size: 

                 719     674     629
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     929    1295    1946    2022 
   Size:        929     366     651      76

  Fragments arranged by size: 

                 929     651     366      76
 
MscI TGG'CCA

Cuts at:      0     703     871    1099    1162    1291    1318    2022 
   Size:        703     168     228      63     129      27     704

  Fragments arranged by size: 

                 704     703     228     168     129      63      27
 
MslI CAynn'nnrTG

Cuts at:      0      64     473    1324    1516    1566    2022 
   Size:         64     409     851     192      50     456

  Fragments arranged by size: 

                 851     456     409     192      64      50
 
MspA1I CmG'CkG

Cuts at:      0     176     453     642     932     966    1844    2022 
   Size:        176     277     189     290      34     878     178

  Fragments arranged by size: 

                 878     290     277     189     178     176      34
 
NarI GG'CG_CC

Cuts at:      0     675    1304    2022 
   Size:        675     629     718

  Fragments arranged by size: 

                 718     675     629
 
NcoI C'CATG_G

Cuts at:      0      83    1286    1526    1640    2022 
   Size:         83    1203     240     114     382

  Fragments arranged by size: 

                1203     382     240     114      83
 
NspI r_CATG'y

Cuts at:      0     649    2022 
   Size:        649    1373
 
PasI CC'CwG_GG

Cuts at:      0    1215    1849    2022 
   Size:       1215     634     173

  Fragments arranged by size: 

                1215     634     173
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1721    2022 
   Size:       1721     301
 
PfoI T'CCnGG_A

Cuts at:      0     386    1832    2022 
   Size:        386    1446     190

  Fragments arranged by size: 

                1446     386     190
 
PmlI CAC'GTG

Cuts at:      0    1326    1361    2022 
   Size:       1326      35     661

  Fragments arranged by size: 

                1326     661      35
 
PpuMI rG'GwC_Cy

Cuts at:      0     300    2022 
   Size:        300    1722
 
PshAI GACnn'nnGTC

Cuts at:      0     139     313    2022 
   Size:        139     174    1709

  Fragments arranged by size: 

                1709     174     139
 
PspOMI G'GGCC_C

Cuts at:      0    1074    2022 
   Size:       1074     948
 
PstI C_TGCA'G

Cuts at:      0     266     404     818     833    1859    2022 
   Size:        266     138     414      15    1026     163

  Fragments arranged by size: 

                1026     414     266     163     138      15
 
PvuII CAG'CTG

Cuts at:      0     966    2022 
   Size:        966    1056
 
SacI G_AGCT'C

Cuts at:      0     758    2022 
   Size:        758    1264
 
SacII CC_GC'GG

Cuts at:      0     933    1845    2022 
   Size:        933     912     177

  Fragments arranged by size: 

                 933     912     177
 
SapI GCTCTTCn'nnn_

Cuts at:      0     550    2022 
   Size:        550    1472
 
SbfI CC_TGCA'GG

Cuts at:      0     404     833    2022 
   Size:        404     429    1189

  Fragments arranged by size: 

                1189     429     404
 
SfcI C'TryA_G

Cuts at:      0     262     400     739     814     829    1855    2022 
   Size:        262     138     339      75      15    1026     167

  Fragments arranged by size: 

                1026     339     262     167     138      75      15
 
SfoI GGC'GCC

Cuts at:      0     676    1305    2022 
   Size:        676     629     717

  Fragments arranged by size: 

                 717     676     629
 
SmaI CCC'GGG

Cuts at:      0    1756    2022 
   Size:       1756     266
 
SmlI C'TyrA_G

Cuts at:      0     304     535    1234    2022 
   Size:        304     231     699     788

  Fragments arranged by size: 

                 788     699     304     231
 
SnaBI TAC'GTA

Cuts at:      0    1548    2022 
   Size:       1548     474
 
SphI G_CATG'C

Cuts at:      0     649    2022 
   Size:        649    1373
 
StyI C'CwwG_G

Cuts at:      0      83     311     985    1286    1448    1526    1574    1640 
   Size:         83     228     674     301     162      78      48      66

Cuts at:   1640    2022 
   Size:        382

  Fragments arranged by size: 

                 674     382     301     228     162      83      78      66
                  48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1014    2022 
   Size:       1014    1008
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      81     435    2022 
   Size:         81     354    1587

  Fragments arranged by size: 

                1587     354      81
 
XmaI C'CCGG_G

Cuts at:      0    1754    2022 
   Size:       1754     268
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AvaI     BanI    BanII     BbeI     BbsI     BglI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
     FspI    FspAI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII
     SapI     SbfI     SfcI     SfoI     SmaI     SmlI    SnaBI     SphI
     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmtI     BplI    BsaBI    BsaWI    BsiWI     BsmI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I
     ClaI    CspCI     DraI   DraIII     DrdI     EciI    EcoRI     FseI
  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI
     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SpeI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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