Restriction map for pF1KB3640
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        BalI        BbrPI       
BbsI        BcgI        BciVI       BfrI        BglI        
BlpI        Bpu1102I    BpuAI       BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoT22I     FseI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcsI          875  GAATTC               [GA]^AATT[CT]
AcyI          151  GGCGCC               G[GA]^CG[CT]C
ApoI          875  GAATTC               [GA]^AATT[CT]
AvaI          416  CCCGAG               C^[CT]CG[GA]G
AviII        1152  TGCGCA               TGC^GCA
AvrII         681  CCTAGG               C^CTAGG
BamHI           7  GGATCC               G^GATCC
BanI          151  GGCGCC               G^G[CT][GA]CC
BanII         593  GGGCTC               G[GA]GC[CT]^C
BbeI          151  GGCGCC               GGCGC^C
BbvCI         659  CCTCAGC              CCTCAGC(-5/-2)
BclI          774  TGATCA               T^GATCA
BfaI          682  CTAG                 C^TAG
BlnI          681  CCTAGG               C^CTAGG
BsaAI         482  CACGTA               [CT]AC^GT[GA]
BsaBI         991  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         151  GGCGCC               G[GA]^CG[CT]C
BsaI          833  GAGACC               GGTCTC(1/5)
BsmAI         833  GAGAC                GTCTC(1/5)
BsoBI         416  CCCGAG               C^[CT]CG[GA]G
BspMI          69  GCAGGT               ACCTGC(4/8)
BstAPI        162  GCAGTTCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI          517  TTTAAA               TTT^AAA
DraII         431  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EarI         1009  GAAGAG               CTCTTC(1/4)
EcoO109I      431  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         875  GAATTC               G^AATTC
EcoRV        1235  GATATC               GAT^ATC
EcoT14I       681  CCTAGG               C^C[AT][AT]GG
FbaI          774  TGATCA               T^GATCA
FspI         1152  TGCGCA               TGC^GCA
Hin1I         151  GGCGCC               G[GA]^CG[CT]C
KasI          151  GGCGCC               G^GCGCC
Ksp632I      1009  GAAGAG               CTCTTC(1/4)
MaeI          682  CTAG                 C^TAG
MaeII         483  ACGT                 A^CGT
MamI          991  GATGATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          518  TTAA                 T^TAA
NarI          151  GGCGCC               GG^CGCC
NspI          848  GCATGC               [GA]CATG^[CT]
PstI          637  CTGCAG               CTGCA^G
PvuII         662  CAGCTG               CAG^CTG
SfcI          637  CTGCAG               C^T[GA][CT]AG
SfoI          151  GGCGCC               GGC^GCC
SphI          848  GCATGC               GCATG^C
StuI           50  AGGCCT               AGG^CCT
StyI          681  CCTAGG               C^C[AT][AT]GG
Tru9I         518  TTAA                 T^TAA
XcmI          788  CCAGTAAAGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          27  CGCG                 CG^CG
               38  CGCG                
AfaI           73  GTAC                 GT^AC
              656  GTAC                
AvaII         471  GGACC                G^G[AT]CC
             1063  GGACC               
BcnI          148  CCCGG                CC^[GC]GG
              185  CCGGG               
BglII         736  AGATCT               A^GATCT
              939  AGATCT              
BmyI          413  GTGCCC               G[AGT]GC[ACT]^C
              593  GGGCTC              
BseRI          96  CTCCTC               GAGGAG(10/8)
             1045  GAGGAG              
BsgI          500  GTGCAG               GTGCAG(16/14)
              897  GTGCAG              
BsmI          804  GAATGC               GAATGC(1/-1)
              914  GCATTC              
Bsp1286I      413  GTGCCC               G[AGT]GC[ACT]^C
              593  GGGCTC              
BspHI         450  TCATGA               T^CATGA
              855  TCATGA              
BstUI          27  CGCG                 CG^CG
               38  CGCG                
EcoNI        1099  CCTGGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1223  CCTACAAGAGG         
FauI         1212  CCCGC                CCCGC(4/6)
                4  GCGGG               
HaeII         151  GGCGCC               [GA]GCGC^[CT]
              234  GGCGCT              
HinfI         686  GAATC                G^A[ACGT]TC
             1141  GATTC               
MslI          393  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              957  CACTCCAGTG          
MspA1I        662  CAGCTG               C[AC]G^C[GT]G
             1213  CCGCTG              
MvnI           27  CGCG                 CG^CG
               38  CGCG                
NciI          148  CCCGG                CC^[GC]GG
              185  CCGGG               
RcaI          450  TCATGA               T^CATGA
              855  TCATGA              
RsaI           73  GTAC                 GT^AC
              656  GTAC                
SmlI          157  CTCAAG               C^T[CT][GA]AG
             1000  CTTGAG              
TfiI          686  GAATC                G^A[AT]TC
             1141  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BpmI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com