Restriction map for pF1KB3640
 (Linear) (Six-base) MAPSORT of: KIBB3640.seq  Check: 8812  from: 1  to: 1257



KIBB3640.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AleI CACnn'nnGTG

Cuts at:      0     961    1257 
   Size:        961     296
 
ApoI r'AATT_y

Cuts at:      0     875    1257 
   Size:        875     382
 
AvaI C'yCGr_G

Cuts at:      0     416    1257 
   Size:        416     841
 
AvrII C'CTAG_G

Cuts at:      0     681    1257 
   Size:        681     576
 
BamHI G'GATC_C

Cuts at:      0       7    1257 
   Size:          7    1250
 
BanI G'GyrC_C

Cuts at:      0     151    1257 
   Size:        151    1106
 
BanII G_rGCy'C

Cuts at:      0     597    1257 
   Size:        597     660
 
BbeI G_GCGC'C

Cuts at:      0     155    1257 
   Size:        155    1102
 
BbvCI CC'TCA_GC

Cuts at:      0     660    1257 
   Size:        660     597
 
BclI T'GATC_A

Cuts at:      0     774    1257 
   Size:        774     483
 
BglII A'GATC_T

Cuts at:      0     736     939    1257 
   Size:        736     203     318

  Fragments arranged by size: 

                 736     318     203
 
Bme1580I G_kGCm'C

Cuts at:      0     417    1257 
   Size:        417     840
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     581     944    1257 
   Size:         67     514     363     313

  Fragments arranged by size: 

                 514     363     313      67
 
Bpu10I CC'TnA_GC

Cuts at:      0     660    1257 
   Size:        660     597
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142    1021    1257 
   Size:        142     879     236

  Fragments arranged by size: 

                 879     236     142
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     827    1257 
   Size:        827     430
 
BsaAI yAC'GTr

Cuts at:      0     484    1257 
   Size:        484     773
 
BsaBI GATnn'nnATC

Cuts at:      0     995    1257 
   Size:        995     262
 
BsaHI Gr'CG_yC

Cuts at:      0     152    1257 
   Size:        152    1105
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      87    1060    1257 
   Size:         87     973     197

  Fragments arranged by size: 

                 973     197      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     521     918    1257 
   Size:        521     397     339

  Fragments arranged by size: 

                 521     397     339
 
BsmI GAATG_Cn'

Cuts at:      0     810     914    1257 
   Size:        810     104     343

  Fragments arranged by size: 

                 810     343     104
 
Bsp1286I G_dGCh'C

Cuts at:      0     417     597    1257 
   Size:        417     180     660

  Fragments arranged by size: 

                 660     417     180
 
BspHI T'CATG_A

Cuts at:      0     450     855    1257 
   Size:        450     405     402

  Fragments arranged by size: 

                 450     405     402
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      60    1257 
   Size:         60    1197
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     168    1257 
   Size:        168    1089
 
BstYI r'GATC_y

Cuts at:      0       7     736     939    1257 
   Size:          7     729     203     318

  Fragments arranged by size: 

                 729     318     203       7
 
BtsI GCAGTG_nn'

Cuts at:      0     803    1257 
   Size:        803     454
 
DraI TTT'AAA

Cuts at:      0     519    1257 
   Size:        519     738
 
EarI CTCTTCn'nnn_

Cuts at:      0    1004    1257 
   Size:       1004     253
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      90    1257 
   Size:         90    1167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     581     944    1257 
   Size:         67     514     363     313

  Fragments arranged by size: 

                 514     363     313      67
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1103    1227    1257 
   Size:       1103     124      30

  Fragments arranged by size: 

                1103     124      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     432    1257 
   Size:        432     825
 
EcoRI G'AATT_C

Cuts at:      0     875    1257 
   Size:        875     382
 
EcoRV GAT'ATC

Cuts at:      0    1237    1257 
   Size:       1237      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     183    1257 
   Size:        183    1074
 
FspI TGC'GCA

Cuts at:      0    1154    1257 
   Size:       1154     103
 
HaeII r_GCGC'y

Cuts at:      0     155     238    1257 
   Size:        155      83    1019

  Fragments arranged by size: 

                1019     155      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     824     856    1097    1129    1221    1253    1257 
   Size:        824      32     241      32      92      32       4

  Fragments arranged by size: 

                 824     241      92      32      32      32       4
 
KasI G'GCGC_C

Cuts at:      0     151    1257 
   Size:        151    1106
 
MslI CAynn'nnrTG

Cuts at:      0     397     961    1257 
   Size:        397     564     296

  Fragments arranged by size: 

                 564     397     296
 
MspA1I CmG'CkG

Cuts at:      0     664    1215    1257 
   Size:        664     551      42

  Fragments arranged by size: 

                 664     551      42
 
NarI GG'CG_CC

Cuts at:      0     152    1257 
   Size:        152    1105
 
NspI r_CATG'y

Cuts at:      0     852    1257 
   Size:        852     405
 
PfoI T'CCnGG_A

Cuts at:      0     338    1257 
   Size:        338     919
 
PstI C_TGCA'G

Cuts at:      0     641    1257 
   Size:        641     616
 
PvuII CAG'CTG

Cuts at:      0     664    1257 
   Size:        664     593
 
SfcI C'TryA_G

Cuts at:      0     637    1257 
   Size:        637     620
 
SfoI GGC'GCC

Cuts at:      0     153    1257 
   Size:        153    1104
 
SmlI C'TyrA_G

Cuts at:      0     157    1000    1257 
   Size:        157     843     257

  Fragments arranged by size: 

                 843     257     157
 
SphI G_CATG'C

Cuts at:      0     852    1257 
   Size:        852     405
 
StuI AGG'CCT

Cuts at:      0      52    1257 
   Size:         52    1205
 
StyI C'CwwG_G

Cuts at:      0     681    1257 
   Size:        681     576
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     795    1257 
   Size:        795     462
 

 Enzymes that do cut:

     AleI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
    BbvCI     BclI    BglII Bme1580I     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI     BsgI     BsmI Bsp1286I    BspHI
    BspMI   BstAPI    BstYI     BtsI     DraI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
     KasI     MslI   MspA1I     NarI     NspI     PfoI     PstI    PvuII
     SfcI     SfoI     SmlI     SphI     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     BaeI     BbsI     BcgI    BciVI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI  EcoICRI     FseI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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