Restriction map for pF1KB3619
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbvCI       
BcgI        BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaHI       BseAI       BseRI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoT22I     
FbaI        FseI        FspI        Hin1I       HpaI        
KasI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PshAI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       SwaI        Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII       1137  ACACGT               A^C[GA][CT]GT
AgeI         1454  ACCGGT               A^CCGGT
AlwNI         240  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2607  CCTGAGG              CC^T[ACGT]AGG
AseI         1318  ATTAAT               AT^TAAT
AsnI         1318  ATTAAT               AT^TAAT
Asp700I      1963  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1944  CTCGGG               C^[CT]CG[GA]G
BalI          753  TGGCCA               TGG^CCA
BciVI        2319  GGATAC               GTATCC(6/5)
BglII        2213  AGATCT               A^GATCT
BsaBI         796  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1133  CTGCAC               GTGCAG(16/14)
BsiEI          17  CGGTCG               CG[GA][CT]^CG
BsoBI        1944  CTCGGG               C^[CT]CG[GA]G
BspHI        1235  TCATGA               T^CATGA
BsrBI          10  CCGCTC               CCGCTC(-3/-3)
BsrFI        1454  ACCGGT               [GA]^CCGG[CT]
Bsu36I       2607  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1454  ACCGGT               [GA]^CCGG[CT]
DraIII       2356  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          753  TGGCCA               [CT]^GGCC[GA]
Eco81I       2607  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2133  CCTAGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1502  GAATTC               G^AATTC
HgaI         2564  GCGTC                GACGC(5/10)
MamI          796  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         753  TGGCCA               TGG^CCA
MscI          753  TGGCCA               TGG^CCA
MslI         2497  CATAGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PinAI        1454  ACCGGT               A^CCGGT
PshBI        1318  ATTAAT               AT^TAAT
PvuII         329  CAGCTG               CAG^CTG
RcaI         1235  TCATGA               T^CATGA
SacI         2293  GAGCTC               GAGCT^C
SapI         2290  GAAGAGC              GCTCTTC(1/4)
ScaI         1829  AGTACT               AGT^ACT
SmlI          843  CTTGAG               C^T[CT][GA]AG
SpeI         2596  ACTAGT               A^CTAGT
SphI         1286  GCATGC               GCATG^C
SspI         1363  AATATT               AAT^ATT
StuI         2611  AGGCCT               AGG^CCT
XmnI         1963  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        398  GGTACC               G^GTACC
             1628  GGTACC              
Asp718I       398  GGTACC               G^GTACC
             1628  GGTACC              
AspHI         846  GAGCAC               G[AT]GC[AT]^C
             2293  GAGCTC              
BbrPI         569  CACGTG               CAC^GTG
             1138  CACGTG              
BpmI          761  CTCCAG               CTGGAG(16/14)
             2296  CTCCAG              
BsaAI         569  CACGTG               [CT]AC^GT[GA]
             1138  CACGTG              
BsaI         2190  GAGACC               GGTCTC(1/5)
              664  GGTCTC              
BsaWI        1454  ACCGGT               [AT]^CCGG[AT]
             1631  ACCGGA              
BsiHKAI       846  GAGCAC               G[AT]GC[AT]^C
             2293  GAGCTC              
BsmFI         266  GGGAC                GGGAC(10/14)
             1204  GTCCC               
BstXI        2271  CCAGACCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2621  CCAACCTTATGG        
BstYI        2213  AGATCT               [GA]^GATC[CT]
             2459  GGATCT              
DraII          66  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             2261  AGGACCT             
DsaI          896  CCATGG               C^C[GA][CT]GG
             1840  CCATGG              
EcoO109I       66  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             2261  AGGACCT             
EcoRV         163  GATATC               GAT^ATC
              811  GATATC              
HaeII         281  AGCGCC               [GA]GCGC^[CT]
             1585  AGCGCC              
HaeIII        754  GGCC                 GG^CC
             2612  GGCC                
HincII        706  GTCAAC               GT[CT]^[GA]AC
             1393  GTTGAC              
HindII        706  GTCAAC               GT[CT]^[GA]AC
             1393  GTTGAC              
HindIII       581  AAGCTT               A^AGCTT
             1385  AAGCTT              
KpnI          398  GGTACC               GGTAC^C
             1628  GGTACC              
MaeII         570  ACGT                 A^CGT
             1139  ACGT                
MflI         2213  AGATCT               [GA]^GATC[CT]
             2459  GGATCT              
MspA1I        329  CAGCTG               C[AC]G^C[GT]G
              655  CAGCGG              
NcoI          896  CCATGG               C^CATGG
             1840  CCATGG              
NspI          153  GCATGT               [GA]CATG^[CT]
             1286  GCATGC              
PflMI         831  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2355  CCACTCTGTGG         
PmaCI         569  CACGTG               CAC^GTG
             1138  CACGTG              
PmlI          569  CACGTG               CAC^GTG
             1138  CACGTG              
PpuMI          66  GGGTCCT              [GA]G^G[AT]CC[CT]
             2261  AGGACCT             
SfcI          902  CTGTAG               C^T[GA][CT]AG
             1022  CTACAG              
Van91I        831  CCAAGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2355  CCACTCTGTGG         
XhoII        2213  AGATCT               [GA]^GATC[CT]
             2459  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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