Restriction map for pF1KB3476
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BalI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BfrI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsmBI       BsmI        BspDI       BspEI       
BspMI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I       1661  GGTACC               G^GTACC
Asp700I      1182  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1661  GGTACC               G^GTACC
BamHI        1956  GGATCC               G^GATCC
BanI         1661  GGTACC               G^G[CT][GA]CC
BclI         1260  TGATCA               T^GATCA
BglII         774  AGATCT               A^GATCT
BsiWI        1450  CGTACG               C^GTACG
Bsp1407I     1966  TGTACA               T^GTACA
BspHI        1005  TCATGA               T^CATGA
BsrBI        2116  GAGCGG               CCGCTC(-3/-3)
BsrGI        1966  TGTACA               T^GTACA
BstBI        1985  TTCGAA               TT^CGAA
CpoI          525  CGGTCCG              CG^G[AT]CCG
DrdI         2074  GACAATAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1072  GAATTC               G^AATTC
EcoRV        1522  GATATC               GAT^ATC
FbaI         1260  TGATCA               T^GATCA
HindIII       641  AAGCTT               A^AGCTT
KpnI         1661  GGTACC               GGTAC^C
MspA1I       1684  CAGCTG               C[AC]G^C[GT]G
NheI         1299  GCTAGC               G^CTAGC
NspV         1985  TTCGAA               TT^CGAA
PvuII        1684  CAGCTG               CAG^CTG
RcaI         1005  TCATGA               T^CATGA
RsrII         525  CGGTCCG              CG^G[AT]CCG
SfuI         1985  TTCGAA               TT^CGAA
SmaI         1358  CCCGGG               CCC^GGG
SnaBI        1448  TACGTA               TAC^GTA
SspBI        1966  TGTACA               T^GTACA
StuI          333  AGGCCT               AGG^CCT
XcmI          882  CCAGAGAATTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1358  CCCGGG               C^CCGGG
XmaI         1358  CCCGGG               C^CCGGG
XmnI         1182  GAACATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AspHI         470  GTGCTC               G[AT]GC[AT]^C
             1340  GAGCAC              
AvaI         1155  CTCGGG               C^[CT]CG[GA]G
             1358  CCCGGG              
BglI         1290  GCCTCTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1836  GCCCTTTTGGC         
BmyI          470  GTGCTC               G[AGT]GC[ACT]^C
             1340  GAGCAC              
BsaAI         846  CACGTA               [CT]AC^GT[GA]
             1448  TACGTA              
BsaBI        1800  GATTGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1951  GATCTGGATC          
BseRI           7  GAGGAG               GAGGAG(10/8)
               46  GAGGAG              
BsiHKAI       470  GTGCTC               G[AT]GC[AT]^C
             1340  GAGCAC              
BsmAI         874  GAGAC                GTCTC(1/5)
             2072  GAGAC               
BsoBI        1155  CTCGGG               C^[CT]CG[GA]G
             1358  CCCGGG              
Bsp1286I      470  GTGCTC               G[AGT]GC[ACT]^C
             1340  GAGCAC              
BspLU11I      557  ACATGT               A^CATGT
             1184  ACATGT              
BsrDI         587  GCAATG               GCAATG(2/0)
              682  GCAATG              
DraI          822  TTTAAA               TTT^AAA
             1480  TTTAAA              
EaeI          338  TGGCCG               [CT]^GGCC[GA]
             1267  CGGCCA              
EarI          597  CTCTTC               CTCTTC(1/4)
               70  GAAGAG              
Ksp632I       597  CTCTTC               CTCTTC(1/4)
               70  GAAGAG              
MaeIII        253  GTCAC                ^GT[ACGT]AC
             1117  GTCAC               
MamI         1800  GATTGCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1951  GATCTGGATC          
NcoI          491  CCATGG               C^CATGG
             1403  CCATGG              
NspI          557  ACATGT               [GA]CATG^[CT]
             1184  ACATGT              
PflMI         845  CCACGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1857  CCAGGTAATGG         
SmlI          172  CTTGAG               C^T[CT][GA]AG
              424  CTCAAG              
Tsp45I        253  GTCAC                ^GT[GC]AC
             1117  GTCAC               
Van91I        845  CCACGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1857  CCAGGTAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AvaII       BbvI        BcnI        
BfaI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       
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