Restriction map for pF1KB3300
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AflIII      AgeI        Alw44I      AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BbrPI       BbsI        BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SexAI       SfiI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tth111I     XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          556  GTCTAC               GT^[AC][GT]AC
AhdI         1486  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1520  AGCGCT               AGC^GCT
Asp700I       750  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1486  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          815  GGCGCC               GGCGC^C
BbvCI        1530  CCTCAGC              CCTCAGC(-5/-2)
BfaI          256  CTAG                 C^TAG
BglII         258  AGATCT               A^GATCT
BsgI         2008  GTGCAG               GTGCAG(16/14)
BsmBI        1836  CGTCTC               CGTCTC(1/5)
BssSI        1858  CACGAG               CACGAG(-5/-1)
Eam1105I     1486  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1520  AGCGCT               AGC^GCT
EcoT22I      1399  ATGCAT               ATGCA^T
KasI          815  GGCGCC               G^GCGCC
KspI          565  CCGCGG               CCGC^GG
MaeI          256  CTAG                 C^TAG
MslI         1203  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          583  GCCGGC               GCC^GGC
NarI          815  GGCGCC               GG^CGCC
NcoI          551  CCATGG               C^CATGG
NgoMIV        583  GCCGGC               G^CCGGC
NsiI         1399  ATGCAT               ATGCA^T
NspI          151  ACATGC               [GA]CATG^[CT]
PaeR7I        802  CTCGAG               C^TCGAG
PflMI        1716  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1817  GGGACCT              [GA]G^G[AT]CC[CT]
SacII         565  CCGCGG               CCGC^GG
SbfI         1338  CCTGCAGG             CCTGCA^GG
ScaI          650  AGTACT               AGT^ACT
SfoI          815  GGCGCC               GGC^GCC
Sse8387I     1338  CCTGCAGG             CCTGCA^GG
Van91I       1716  CCAGCTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          255  TCTAGA               T^CTAGA
XhoI          802  CTCGAG               C^TCGAG
XmnI          750  GAACAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII         566  CGCG                 CG^CG
             1350  CGCG                
AccIII         14  TCCGGA               T^CCGGA
             1868  TCCGGA              
ApaI          709  GGGCCC               GGGCC^C
             1526  GGGCCC              
AvaI          692  CTCGGG               C^[CT]CG[GA]G
              802  CTCGAG              
AviII        1770  TGCGCA               TGC^GCA
             2108  TGCGCA              
BalI         1823  TGGCCA               TGG^CCA
             2070  TGGCCA              
BamHI        1013  GGATCC               G^GATCC
             1901  GGATCC              
BanI          580  GGTGCC               G^G[CT][GA]CC
              815  GGCGCC              
BcnI          911  CCCGG                CC^[GC]GG
             1587  CCGGG               
BsaWI          14  TCCGGA               [AT]^CCGG[AT]
             1868  TCCGGA              
BseAI          14  TCCGGA               T^CCGGA
             1868  TCCGGA              
BsoBI         692  CTCGGG               C^[CT]CG[GA]G
              802  CTCGAG              
BspEI          14  TCCGGA               T^CCGGA
             1868  TCCGGA              
BsrFI         583  GCCGGC               [GA]^CCGG[CT]
             1196  ACCGGC              
BstAPI       1167  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1752  GCACTACCTGC         
BstBI         446  TTCGAA               TT^CGAA
              515  TTCGAA              
BstUI         566  CGCG                 CG^CG
             1350  CGCG                
Cfr10I        583  GCCGGC               [GA]^CCGG[CT]
             1196  ACCGGC              
EcoNI        1728  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1821  CCTGGCCAAGG         
EcoT14I       551  CCATGG               C^C[AT][AT]GG
             1826  CCAAGG              
FspI         1770  TGCGCA               TGC^GCA
             2108  TGCGCA              
MluNI        1823  TGGCCA               TGG^CCA
             2070  TGGCCA              
MroI           14  TCCGGA               T^CCGGA
             1868  TCCGGA              
MscI         1823  TGGCCA               TGG^CCA
             2070  TGGCCA              
MvnI          566  CGCG                 CG^CG
             1350  CGCG                
NciI          911  CCCGG                CC^[GC]GG
             1587  CCGGG               
NspV          446  TTCGAA               TT^CGAA
              515  TTCGAA              
SacI          118  GAGCTC               GAGCT^C
             1582  GAGCTC              
SapI         1579  GAAGAGC              GCTCTTC(1/4)
                6  GCTCTTC             
SfuI          446  TTCGAA               TT^CGAA
              515  TTCGAA              
SmlI          726  CTCAAG               C^T[CT][GA]AG
              802  CTCGAG              
StuI         1247  AGGCCT               AGG^CCT
             2012  AGGCCT              
StyI          551  CCATGG               C^C[AT][AT]GG
             1826  CCAAGG              
Tsp509I       663  AATT                 ^AATT
             1636  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaII       BanII       BbvI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
TspRI       TthHB8I     XhoII       
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