Restriction map for pF1KB3244
##################################
# NO CUT ENZYMES (   46 enzymes) #
##################################

AccIII      AclI        AgeI        AocI        ApaI        
AscI        AseI        AsnI        BbsI        BpuAI       
BsaWI       BseAI       BspEI       BssHII      Bst1107I    
BstZ17I     Bsu36I      CpoI        DraI        EagI        
EclXI       Eco52I      Eco81I      EcoT22I     FseI        
HpaI        MfeI        MluI        MroI        MunI        
NotI        NruI        NsiI        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SbfI        SgrAI       Sse8387I    SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        5866  GACGTC               GACGT^C
AflII         654  CTTAAG               C^TTAAG
AhdI         3019  GACCTCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      2949  GAACCGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3019  GACCTCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        4128  CCTAGG               C^CTAGG
BbeI         4387  GGCGCC               GGCGC^C
BbrPI        4651  CACGTG               CAC^GTG
BciVI         697  GTATCC               GTATCC(6/5)
BfrI          654  CTTAAG               C^TTAAG
BlnI         4128  CCTAGG               C^CTAGG
BsaBI        2808  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        2307  CGACCG               CG[GA][CT]^CG
BsiWI        3459  CGTACG               C^GTACG
Bsp1407I     6443  TGTACA               T^GTACA
BspHI        6323  TCATGA               T^CATGA
BspLU11I     2762  ACATGT               A^CATGT
BsrDI        3420  CATTGC               GCAATG(2/0)
BsrGI        6443  TGTACA               T^GTACA
BssSI        1729  CTCGTG               CACGAG(-5/-1)
BstBI        2600  TTCGAA               TT^CGAA
Eam1105I     3019  GACCTCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        6193  GAATTC               G^AATTC
KasI         4387  GGCGCC               G^GCGCC
KspI         6174  CCGCGG               CCGC^GG
MamI         2808  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         4387  GGCGCC               GG^CGCC
NdeI         4644  CATATG               CA^TATG
NspV         2600  TTCGAA               TT^CGAA
PmaCI        4651  CACGTG               CAC^GTG
PmlI         4651  CACGTG               CAC^GTG
RcaI         6323  TCATGA               T^CATGA
SacII        6174  CCGCGG               CCGC^GG
SalI           63  GTCGAC               G^TCGAC
SapI         2088  GAAGAGC              GCTCTTC(1/4)
ScaI         3661  AGTACT               AGT^ACT
SexAI        4239  ACCAGGT              A^CC[AT]GGT
SfoI         4387  GGCGCC               GGC^GCC
SfuI         2600  TTCGAA               TT^CGAA
SnaBI        1391  TACGTA               TAC^GTA
SpeI         5290  ACTAGT               A^CTAGT
SspBI        6443  TGTACA               T^GTACA
SspI         1306  AATATT               AAT^ATT
StuI         2132  AGGCCT               AGG^CCT
XmnI         2949  GAACCGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII       2762  ACATGT               A^C[GA][CT]GT
             3462  ACGTGT              
Aor51HI      3134  AGCGCT               AGC^GCT
             4631  AGCGCT              
AspI         1342  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5866  GACGTCGTC           
AviII        2150  TGCGCA               TGC^GCA
             6137  TGCGCA              
BamHI         530  GGATCC               G^GATCC
             6402  GGATCC              
BcgI          571  CGATGCCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4851  CGACTGCTTTGC        
BglII         143  AGATCT               A^GATCT
             2789  AGATCT              
BspDI        4093  ATCGAT               AT^CGAT
             5194  ATCGAT              
ClaI         4093  ATCGAT               AT^CGAT
             5194  ATCGAT              
DraIII       2185  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3497  CACTGGGTG           
DrdI           22  GACGGGTCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4516  GACAGCGTGGTC        
Eco47III     3134  AGCGCT               AGC^GCT
             4631  AGCGCT              
FspI         2150  TGCGCA               TGC^GCA
             6137  TGCGCA              
NheI         1935  GCTAGC               G^CTAGC
             3960  GCTAGC              
PaeR7I       2824  CTCGAG               C^TCGAG
             3022  CTCGAG              
PflFI        1342  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5866  GACGTCGTC           
SfiI         1890  GGCCAGTAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             6159  GGCCTTCGTGGCC       
SmaI         5217  CCCGGG               CCC^GGG
             6329  CCCGGG              
SphI         3369  GCATGC               GCATG^C
             6140  GCATGC              
Tth111I      1342  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5866  GACGTCGTC           
XhoI         2824  CTCGAG               C^TCGAG
             3022  CTCGAG              
XmaCI        5217  CCCGGG               C^CCGGG
             6329  CCCGGG              
XmaI         5217  CCCGGG               C^CCGGG
             6329  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  144 enzymes) #
#########################################

Acc65I      AccI        AccII       AciI        AcsI        
AcyI        AfaI        AluI        Alw44I      AlwI        
AlwNI       ApaLI       ApoI        Asp718I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BclI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoRV       EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com