Restriction map for pF1KB3244
 (Linear) (Six-base) MAPSORT of: KIBB3244.seq  Check: 4564  from: 1  to: 6486



KIBB3244.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3274    4238    5042    5482    6486 
   Size:       3274     964     804     440    1004

  Fragments arranged by size: 

                3274    1004     964     804     440
 
AatII G_ACGT'C

Cuts at:      0    5870    6486 
   Size:       5870     616
 
AccI GT'mk_AC

Cuts at:      0      64     356    1769    2636    6486 
   Size:         64     292    1413     867    3850

  Fragments arranged by size: 

                3850    1413     867     292      64
 
Acc65I G'GTAC_C

Cuts at:      0     474     606    1646    3056    5211    6486 
   Size:        474     132    1040    1410    2155    1275

  Fragments arranged by size: 

                2155    1410    1275    1040     474     132
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     975    1013    1288    1801    2855    2948    4253    4495 
   Size:        975      38     275     513    1054      93    1305     242

Cuts at:   4495    5710    6073    6486 
   Size:       1215     363     413

  Fragments arranged by size: 

                1305    1215    1054     975     513     413     363     275
                 242      93      38
 
AfeI AGC'GCT

Cuts at:      0    3136    4633    6486 
   Size:       3136    1497    1853

  Fragments arranged by size: 

                3136    1853    1497
 
AflII C'TTAA_G

Cuts at:      0     654    6486 
   Size:        654    5832
 
AflIII A'CryG_T

Cuts at:      0    2762    3462    6486 
   Size:       2762     700    3024

  Fragments arranged by size: 

                3024    2762     700
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3024    6486 
   Size:       3024    3462
 
AleI CACnn'nnGTG

Cuts at:      0     293    1513    4306    5056    6040    6229    6486 
   Size:        293    1220    2793     750     984     189     257

  Fragments arranged by size: 

                2793    1220     984     750     293     257     189
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1969    2071    3437    6486 
   Size:       1969     102    1366    3049

  Fragments arranged by size: 

                3049    1969    1366     102
 
AlwNI CAG_nnn'CTG

Cuts at:      0     290    1335    1633    2490    4282    4374    4384    5208 
   Size:        290    1045     298     857    1792      92      10     824

Cuts at:   5208    5284    5520    5979    6486 
   Size:         76     236     459     507

  Fragments arranged by size: 

                1792    1045     857     824     507     459     298     290
                 236      92      76      10
 
ApaLI G'TGCA_C

Cuts at:      0     970    4104    4225    4639    6486 
   Size:        970    3134     121     414    1847

  Fragments arranged by size: 

                3134    1847     970     414     121
 
ApoI r'AATT_y

Cuts at:      0    1231    1605    2659    6193    6486 
   Size:       1231     374    1054    3534     293

  Fragments arranged by size: 

                3534    1231    1054     374     293
 
AvaI C'yCGr_G

Cuts at:      0     109     234     362    1242    1590    2824    3022    5217 
   Size:        109     125     128     880     348    1234     198    2195

Cuts at:   5217    6329    6486 
   Size:       1112     157

  Fragments arranged by size: 

                2195    1234    1112     880     348     198     157     128
                 125     109
 
AvrII C'CTAG_G

Cuts at:      0    4128    6486 
   Size:       4128    2358
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     590     623    3048    3081    6486 
   Size:        590      33    2425      33    3405

  Fragments arranged by size: 

                3405    2425     590      33      33
 
BamHI G'GATC_C

Cuts at:      0     530    6402    6486 
   Size:        530    5872      84

  Fragments arranged by size: 

                5872     530      84
 
BanI G'GyrC_C

Cuts at:      0     474     520     606    1646    2578    2917    3056    3597 
   Size:        474      46      86    1040     932     339     139     541

Cuts at:   3597    4387    4447    5152    5211    5470    5882    6123    6178 
   Size:        790      60     705      59     259     412     241      55

Cuts at:   6178    6486 
   Size:        308

  Fragments arranged by size: 

                1040     932     790     705     541     474     412     339
                 308     259     241     139      86      60      59      55
                  46
 
BanII G_rGCy'C

Cuts at:      0     853     927    1201    1249    1693    1721    1964    2297 
   Size:        853      74     274      48     444      28     243     333

Cuts at:   2297    2825    3014    6486 
   Size:        528     189    3472

  Fragments arranged by size: 

                3472     853     528     444     333     274     243     189
                  74      48      28
 
BbeI G_GCGC'C

Cuts at:      0    4391    6486 
   Size:       4391    2095
 
BbvCI CC'TCA_GC

Cuts at:      0     593    5264    5277    5405    6486 
   Size:        593    4671      13     128    1081

  Fragments arranged by size: 

                4671    1081     593     128      13
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     560     594    4840    4874    6486 
   Size:        560      34    4246      34    1612

  Fragments arranged by size: 

                4246    1612     560      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     708    6486 
   Size:        708    5778
 
BclI T'GATC_A

Cuts at:      0    1295    2181    5936    6486 
   Size:       1295     886    3755     550

  Fragments arranged by size: 

                3755    1295     886     550
 
BglI GCCn_nnn'nGGC

Cuts at:      0     787    1564    1897    6166    6486 
   Size:        787     777     333    4269     320

  Fragments arranged by size: 

                4269     787     777     333     320
 
BglII A'GATC_T

Cuts at:      0     143    2789    6486 
   Size:        143    2646    3697

  Fragments arranged by size: 

                3697    2646     143
 
BlpI GC'TnA_GC

Cuts at:      0    3088    3721    4619    4978    5785    6486 
   Size:       3088     633     898     359     807     701

  Fragments arranged by size: 

                3088     898     807     701     633     359
 
Bme1580I G_kGCm'C

Cuts at:      0     525     974    1510    2581    2920    3934    4085    4108 
   Size:        525     449     536    1071     339    1014     151      23

Cuts at:   4108    4229    4450    4643    5155    6486 
   Size:        121     221     193     512    1331

  Fragments arranged by size: 

                1331    1071    1014     536     525     512     449     339
                 221     193     151     121      23
 
BmgBI CAC'GTC

Cuts at:      0    3219    3636    6486 
   Size:       3219     417    2850

  Fragments arranged by size: 

                3219    2850     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     683     930    2500    3508    3674    6090    6486 
   Size:        683     247    1570    1008     166    2416     396

  Fragments arranged by size: 

                2416    1570    1008     683     396     247     166
 
BmtI G_CTAG'C

Cuts at:      0    1939    3964    6486 
   Size:       1939    2025    2522

  Fragments arranged by size: 

                2522    2025    1939
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     921    1737    4515    4821    5442    6486 
   Size:        921     816    2778     306     621    1044

  Fragments arranged by size: 

                2778    1044     921     816     621     306
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312    1247    3151    3367    3599    3835    3973    4780 
   Size:        312     935    1904     216     232     236     138     807

Cuts at:   4780    5092    5915    6486 
   Size:        312     823     571

  Fragments arranged by size: 

                1904     935     823     807     571     312     312     236
                 232     216     138
 
Bpu10I CC'TnA_GC

Cuts at:      0     593     777    1200    1958    1963    2044    3142    3206 
   Size:        593     184     423     758       5      81    1098      64

Cuts at:   3206    4744    4796    5264    5277    5405    6486 
   Size:       1538      52     468      13     128    1081

  Fragments arranged by size: 

                1538    1098    1081     758     593     468     423     184
                 128      81      64      52      13       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    3694    6486 
   Size:        538    3156    2792

  Fragments arranged by size: 

                3156    2792     538
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     217     401     489     652    1513    3119    4486    4927 
   Size:        217     184      88     163     861    1606    1367     441

Cuts at:   4927    6486 
   Size:       1559

  Fragments arranged by size: 

                1606    1559    1367     861     441     217     184     163
                  88
 
BsaAI yAC'GTr

Cuts at:      0     246    1393    1841    3463    4653    6120    6486 
   Size:        246    1147     448    1622    1190    1467     366

  Fragments arranged by size: 

                1622    1467    1190    1147     448     366     246
 
BsaBI GATnn'nnATC

Cuts at:      0    2812    6486 
   Size:       2812    3674
 
BsaHI Gr'CG_yC

Cuts at:      0    1820    2633    3878    4020    4346    4388    5867    6486 
   Size:       1820     813    1245     142     326      42    1479     619

  Fragments arranged by size: 

                1820    1479    1245     813     619     326     142      42
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      10      40     664     694    1568    1598    1786    1816 
   Size:         10      30     624      30     874      30     188      30

Cuts at:   1816    3581    3611    4879    4909    5065    5095    5765    5795 
   Size:       1765      30    1268      30     156      30     670      30

Cuts at:   5795    6486 
   Size:        691

  Fragments arranged by size: 

                1765    1268     874     691     670     624     188     156
                  30      30      30      30      30      30      30      30
                  10
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     917    1729    2143    3925    4681    4903    5422    6019 
   Size:        917     812     414    1782     756     222     519     597

Cuts at:   6019    6223    6486 
   Size:        204     263

  Fragments arranged by size: 

                1782     917     812     756     597     519     414     263
                 222     204
 
BseYI C'CCAG_C

Cuts at:      0     283     402    1084    1641    1737    2002    2351    2913 
   Size:        283     119     682     557      96     265     349     562

Cuts at:   2913    4009    4291    5092    5769    5888    5944    6009    6130 
   Size:       1096     282     801     677     119      56      65     121

Cuts at:   6130    6474    6486 
   Size:        344      12

  Fragments arranged by size: 

                1096     801     682     677     562     557     349     344
                 283     282     265     121     119     119      96      65
                  56      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1339    1685    2305    5524    5548    5683    5863    6486 
   Size:       1339     346     620    3219      24     135     180     623

  Fragments arranged by size: 

                3219    1339     623     620     346     180     135      24
            
 
BsiEI CG_ry'CG

Cuts at:      0    2310    6486 
   Size:       2310    4176
 
BsiHKAI G_wGCw'C

Cuts at:      0     155     974    1016    1721    1964    2297    2458    2825 
   Size:        155     819      42     705     243     333     161     367

Cuts at:   2825    3014    4108    4229    4643    5731    5786    5879    5966 
   Size:        189    1094     121     414    1088      55      93      87

Cuts at:   5966    6236    6486 
   Size:        270     250

  Fragments arranged by size: 

                1094    1088     819     705     414     367     333     270
                 250     243     189     161     155     121      93      87
                  55      42
 
BsiWI C'GTAC_G

Cuts at:      0    3459    6486 
   Size:       3459    3027
 
BsmI GAATG_Cn'

Cuts at:      0    1866    3285    3291    3457    6486 
   Size:       1866    1419       6     166    3029

  Fragments arranged by size: 

                3029    1866    1419     166       6
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1811    3165    4027    6486 
   Size:       1811    1354     862    2459

  Fragments arranged by size: 

                2459    1811    1354     862
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     525     853     927     974    1016    1201    1249 
   Size:        155     370     328      74      47      42     185      48

Cuts at:   1249    1510    1693    1721    1964    2297    2458    2581    2825 
   Size:        261     183      28     243     333     161     123     244

Cuts at:   2825    2920    3014    3934    4085    4108    4229    4450    4643 
   Size:         95      94     920     151      23     121     221     193

Cuts at:   4643    5155    5731    5786    5879    5966    6236    6486 
   Size:        512     576      55      93      87     270     250

  Fragments arranged by size: 

                 920     576     512     370     333     328     270     261
                 250     244     243     221     193     185     183     161
                 155     151     123     121      95      94      93      87
                  74      55      48      47      42      28      23
 
BspHI T'CATG_A

Cuts at:      0    6323    6486 
   Size:       6323     163
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2402    3274    4238    4723    5000    5042    5193    5482 
   Size:       2402     872     964     485     277      42     151     289

Cuts at:   5482    6486 
   Size:       1004

  Fragments arranged by size: 

                2402    1004     964     872     485     289     277     151
                  42
 
BsrBI CCG'CTC

Cuts at:      0    1116    3092    3351    5673    6486 
   Size:       1116    1976     259    2322     813

  Fragments arranged by size: 

                2322    1976    1116     813     259
 
BsrDI GCAATG_nn'

Cuts at:      0    3419    6486 
   Size:       3419    3067
 
BsrFI r'CCGG_y

Cuts at:      0      91    3676    4016    4973    6486 
   Size:         91    3585     340     957    1513

  Fragments arranged by size: 

                3585    1513     957     340      91
 
BsrGI T'GTAC_A

Cuts at:      0    6443    6486 
   Size:       6443      43
 
BssSI C'ACGA_G

Cuts at:      0    1729    6486 
   Size:       1729    4757
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1672    5004    6363    6486 
   Size:       1672    3332    1359     123

  Fragments arranged by size: 

                3332    1672    1359     123
 
BstBI TT'CG_AA

Cuts at:      0    2601    6486 
   Size:       2601    3885
 
BstEII G'GTnAC_C

Cuts at:      0     670    3312    6046    6486 
   Size:        670    2642    2734     440

  Fragments arranged by size: 

                2734    2642     670     440
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      49     883     920    2877    3345    3498    6474    6486 
   Size:         49     834      37    1957     468     153    2976      12

  Fragments arranged by size: 

                2976    1957     834     468     153      49      37      12
            
 
BstYI r'GATC_y

Cuts at:      0     143     530     566     749    2495    2789    3737    4670 
   Size:        143     387      36     183    1746     294     948     933

Cuts at:   4670    5067    6402    6486 
   Size:        397    1335      84

  Fragments arranged by size: 

                1746    1335     948     933     397     387     294     183
                 143      84      36
 
BtgI C'CryG_G

Cuts at:      0     499     882    2370    2852    2882    3050    3176    3227 
   Size:        499     383    1488     482      30     168     126      51

Cuts at:   3227    4304    4451    4595    5325    5759    6174    6486 
   Size:       1077     147     144     730     434     415     312

  Fragments arranged by size: 

                1488    1077     730     499     482     434     415     383
                 312     168     147     144     126      51      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2325    3240    6486 
   Size:       2325     915    3246

  Fragments arranged by size: 

                3246    2325     915
 
BtsI GCAGTG_nn'

Cuts at:      0     907    2513    2908    3332    5647    5906    5919    6486 
   Size:        907    1606     395     424    2315     259      13     567

  Fragments arranged by size: 

                2315    1606     907     567     424     395     259      13
            
 
ClaI AT'CG_AT

Cuts at:      0    4094    5195    6486 
   Size:       4094    1101    1291

  Fragments arranged by size: 

                4094    1291    1101
 
DraIII CAC_nnn'GTG

Cuts at:      0    2190    3502    6486 
   Size:       2190    1312    2984

  Fragments arranged by size: 

                2984    2190    1312
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      28    4522    6486 
   Size:         28    4494    1964

  Fragments arranged by size: 

                4494    1964      28
 
EaeI y'GGCC_r

Cuts at:      0    1218    1337    1889    2539    2879    3521    3674    4064 
   Size:       1218     119     552     650     340     642     153     390

Cuts at:   4064    4454    5245    5349    5909    6486 
   Size:        390     791     104     560     577

  Fragments arranged by size: 

                1218     791     650     642     577     560     552     390
                 390     340     153     119     104
 
EarI CTCTTCn'nnn_

Cuts at:      0     655     937    2083    4032    6486 
   Size:        655     282    1146    1949    2454

  Fragments arranged by size: 

                2454    1949    1146     655     282
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    5149    6412    6486 
   Size:       5149    1263      74

  Fragments arranged by size: 

                5149    1263      74
 
EcoICRI GAG'CTC

Cuts at:      0    1719    1962    2295    2823    3012    6486 
   Size:       1719     243     333     528     189    3474

  Fragments arranged by size: 

                3474    1719     528     333     243     189
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     975    1013    1247    1288    1801    2855    2948 
   Size:        312     663      38     234      41     513    1054      93

Cuts at:   2948    3151    3367    3599    3835    3973    4253    4495    4780 
   Size:        203     216     232     236     138     280     242     285

Cuts at:   4780    5092    5710    5915    6073    6486 
   Size:        312     618     205     158     413

  Fragments arranged by size: 

                1054     663     618     513     413     312     312     285
                 280     242     236     234     232     216     205     203
                 158     138      93      41      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1270    1707    5132    6486 
   Size:       1270     437    3425    1354

  Fragments arranged by size: 

                3425    1354    1270     437
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114    1206    1263    1500    2040    2145    3400    4125 
   Size:        114    1092      57     237     540     105    1255     725

Cuts at:   4125    4418    4771    4907    5525    5976    6486 
   Size:        293     353     136     618     451     510

  Fragments arranged by size: 

                1255    1092     725     618     540     510     451     353
                 293     237     136     114     105      57
 
EcoRI G'AATT_C

Cuts at:      0    6193    6486 
   Size:       6193     293
 
EcoRV GAT'ATC

Cuts at:      0    1053    3330    4098    6486 
   Size:       1053    2277     768    2388

  Fragments arranged by size: 

                2388    2277    1053     768
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4278    6486 
   Size:       4278    2208
 
FspI TGC'GCA

Cuts at:      0    2152    6139    6486 
   Size:       2152    3987     347

  Fragments arranged by size: 

                3987    2152     347
 
HaeII r_GCGC'y

Cuts at:      0     861    1131    3138    4391    4635    4767    6217    6486 
   Size:        861     270    2007    1253     244     132    1450     269

  Fragments arranged by size: 

                2007    1450    1253     861     270     269     244     132
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     664     696    1137    1169    1524    1556 
   Size:         10      32     622      32     441      32     355      32

Cuts at:   1556    2776    2808    3010    3042    5605    5637    6486 
   Size:       1220      32     202      32    2563      32     849

  Fragments arranged by size: 

                2563    1220     849     622     441     355     202      32
                  32      32      32      32      32      32      10
 
HincII GTy'rAC

Cuts at:      0      65    4170    6375    6486 
   Size:         65    4105    2205     111

  Fragments arranged by size: 

                4105    2205     111      65
 
HindIII A'AGCT_T

Cuts at:      0    4189    4477    6287    6486 
   Size:       4189     288    1810     199

  Fragments arranged by size: 

                4189    1810     288     199
 
KasI G'GCGC_C

Cuts at:      0    4387    6486 
   Size:       4387    2099
 
KpnI G_GTAC'C

Cuts at:      0     478     610    1650    3060    5215    6486 
   Size:        478     132    1040    1410    2155    1271

  Fragments arranged by size: 

                2155    1410    1271    1040     478     132
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2195    2248    5411    5937    6486 
   Size:       2195      53    3163     526     549

  Fragments arranged by size: 

                3163    2195     549     526      53
 
MscI TGG'CCA

Cuts at:      0    1220    1891    2541    2881    3523    5247    6486 
   Size:       1220     671     650     340     642    1724    1239

  Fragments arranged by size: 

                1724    1239    1220     671     650     642     340
 
MslI CAynn'nnrTG

Cuts at:      0     293    1513    1855    2722    2881    3296    4306    5056 
   Size:        293    1220     342     867     159     415    1010     750

Cuts at:   5056    6040    6229    6451    6486 
   Size:        984     189     222      35

  Fragments arranged by size: 

                1220    1010     984     867     750     415     342     293
                 222     189     159      35
 
MspA1I CmG'CkG

Cuts at:      0    1084    1633    2254    2312    2355    2533    2865    3438 
   Size:       1084     549     621      58      43     178     332     573

Cuts at:   3438    3695    3856    3892    3942    4069    4282    5404    6176 
   Size:        257     161      36      50     127     213    1122     772

Cuts at:   6176    6486 
   Size:        310

  Fragments arranged by size: 

                1122    1084     772     621     573     549     332     310
                 257     213     178     161     127      58      50      43
                  36
 
NaeI GCC'GGC

Cuts at:      0    3678    4018    4975    6486 
   Size:       3678     340     957    1511

  Fragments arranged by size: 

                3678    1511     957     340
 
NarI GG'CG_CC

Cuts at:      0    4388    6486 
   Size:       4388    2098
 
NcoI C'CATG_G

Cuts at:      0     882    2852    2882    4304    5759    6486 
   Size:        882    1970      30    1422    1455     727

  Fragments arranged by size: 

                1970    1455    1422     882     727      30
 
NdeI CA'TA_TG

Cuts at:      0    4645    6486 
   Size:       4645    1841
 
NgoMIV G'CCGG_C

Cuts at:      0    3676    4016    4973    6486 
   Size:       3676     340     957    1513

  Fragments arranged by size: 

                3676    1513     957     340
 
NheI G'CTAG_C

Cuts at:      0    1935    3960    6486 
   Size:       1935    2025    2526

  Fragments arranged by size: 

                2526    2025    1935
 
NspI r_CATG'y

Cuts at:      0    1585    2350    2766    3274    3373    4168    5103    6144 
   Size:       1585     765     416     508      99     795     935    1041

Cuts at:   6144    6444    6486 
   Size:        300      42

  Fragments arranged by size: 

                1585    1041     935     795     765     508     416     300
                  99      42
 
PasI CC'CwG_GG

Cuts at:      0     611    1093    1141    1329    1677    4924    5335    6486 
   Size:        611     482      48     188     348    3247     411    1151

  Fragments arranged by size: 

                3247    1151     611     482     411     348     188      48
            
 
PciI A'CATG_T

Cuts at:      0    2762    6486 
   Size:       2762    3724
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     290     771    1335    3051    4374    4533    4818    4884 
   Size:        290     481     564    1716    1323     159     285      66

Cuts at:   4884    5520    6486 
   Size:        636     966

  Fragments arranged by size: 

                1716    1323     966     636     564     481     290     285
                 159      66
 
PfoI T'CCnGG_A

Cuts at:      0    3197    3950    6486 
   Size:       3197     753    2536

  Fragments arranged by size: 

                3197    2536     753
 
PmlI CAC'GTG

Cuts at:      0    4653    6486 
   Size:       4653    1833
 
PpuMI rG'GwC_Cy

Cuts at:      0    1500    2040    3400    5525    6486 
   Size:       1500     540    1360    2125     961

  Fragments arranged by size: 

                2125    1500    1360     961     540
 
PspXI vC'TCGA_Gb

Cuts at:      0    3022    6486 
   Size:       3022    3464
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    6427    6459    6486 
   Size:       6427      32      27

  Fragments arranged by size: 

                6427      32      27
 
PstI C_TGCA'G

Cuts at:      0     326    3416    4467    5000    6486 
   Size:        326    3090    1051     533    1486

  Fragments arranged by size: 

                3090    1486    1051     533     326
 
PvuII CAG'CTG

Cuts at:      0    1084    2254    2355    2533    2865    3438    3856    3892 
   Size:       1084    1170     101     178     332     573     418      36

Cuts at:   3892    5404    6486 
   Size:       1512    1082

  Fragments arranged by size: 

                1512    1170    1084    1082     573     418     332     178
                 101      36
 
SacI G_AGCT'C

Cuts at:      0    1721    1964    2297    2825    3014    6486 
   Size:       1721     243     333     528     189    3472

  Fragments arranged by size: 

                3472    1721     528     333     243     189
 
SacII CC_GC'GG

Cuts at:      0    6177    6486 
   Size:       6177     309
 
SalI G'TCGA_C

Cuts at:      0      63    6486 
   Size:         63    6423
 
SanDI GG'GwC_CC

Cuts at:      0    5525    6486 
   Size:       5525     961
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2083    6486 
   Size:       2083    4403
 
ScaI AGT'ACT

Cuts at:      0    3663    6486 
   Size:       3663    2823
 
SexAI A'CCwGG_T

Cuts at:      0    4239    6486 
   Size:       4239    2247
 
SfcI C'TryA_G

Cuts at:      0     322    2675    3388    3412    3828    4463    4996    6486 
   Size:        322    2353     713      24     416     635     533    1490

  Fragments arranged by size: 

                2353    1490     713     635     533     416     322      24
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1897    6166    6486 
   Size:       1897    4269     320

  Fragments arranged by size: 

                4269    1897     320
 
SfoI GGC'GCC

Cuts at:      0    4389    6486 
   Size:       4389    2097
 
SmaI CCC'GGG

Cuts at:      0    5219    6331    6486 
   Size:       5219    1112     155

  Fragments arranged by size: 

                5219    1112     155
 
SmlI C'TyrA_G

Cuts at:      0     553     654    2824    3022    3709    6486 
   Size:        553     101    2170     198     687    2777

  Fragments arranged by size: 

                2777    2170     687     553     198     101
 
SnaBI TAC'GTA

Cuts at:      0    1393    6486 
   Size:       1393    5093
 
SpeI A'CTAG_T

Cuts at:      0    5290    6486 
   Size:       5290    1196
 
SphI G_CATG'C

Cuts at:      0    3373    6144    6486 
   Size:       3373    2771     342

  Fragments arranged by size: 

                3373    2771     342
 
SspI AAT'ATT

Cuts at:      0    1308    6486 
   Size:       1308    5178
 
StuI AGG'CCT

Cuts at:      0    2134    6486 
   Size:       2134    4352
 
StyI C'CwwG_G

Cuts at:      0     882    1466    2622    2852    2882    3425    3518    4040 
   Size:        882     584    1156     230      30     543      93     522

Cuts at:   4040    4128    4304    4413    4442    4883    5111    5609    5759 
   Size:         88     176     109      29     441     228     498     150

Cuts at:   5759    6486 
   Size:        727

  Fragments arranged by size: 

                1156     882     727     584     543     522     498     441
                 230     228     176     150     109      93      88      30
                  29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      18     199    1022    1549    1669    2599    2845    3490 
   Size:         18     181     823     527     120     930     246     645

Cuts at:   3490    3634    5986    6034    6109    6162    6486 
   Size:        144    2352      48      75      53     324

  Fragments arranged by size: 

                2352     930     823     645     527     324     246     181
                 144     120      75      53      48      18
 
TatI w'GTAC_w

Cuts at:      0    3661    6069    6443    6486 
   Size:       3661    2408     374      43

  Fragments arranged by size: 

                3661    2408     374      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1568    1600    1893    1925    2597    2629    3581    3613 
   Size:       1568      32     293      32     672      32     952      32

Cuts at:   3613    3913    3945    4813    4845    4879    4904    4911    4936 
   Size:        300      32     868      32      34      25       7      25

Cuts at:   4936    5065    5097    5765    5797    6486 
   Size:        129      32     668      32     689

  Fragments arranged by size: 

                1568     952     868     689     672     668     300     293
                 129      34      32      32      32      32      32      32
                  32      32      25      25       7
 
Tth111I GACn'n_nGTC

Cuts at:      0    1345    5869    6486 
   Size:       1345    4524     617

  Fragments arranged by size: 

                4524    1345     617
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1938    4116    5766    6342    6486 
   Size:       1938    2178    1650     576     144

  Fragments arranged by size: 

                2178    1938    1650     576     144
 
XhoI C'TCGA_G

Cuts at:      0    2824    3022    6486 
   Size:       2824     198    3464

  Fragments arranged by size: 

                3464    2824     198
 
XmaI C'CCGG_G

Cuts at:      0    5217    6329    6486 
   Size:       5217    1112     157

  Fragments arranged by size: 

                5217    1112     157
 
XmnI GAAnn'nnTTC

Cuts at:      0    2953    6486 
   Size:       2953    3533
 
ZraI GAC'GTC

Cuts at:      0    5868    6486 
   Size:       5868     618
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI    PspXI     PsrI
     PstI    PvuII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AgeI     AloI     ApaI     AscI     AseI    AsiSI     BbsI
    BfrBI    BsaWI    BspEI   BssHII  BstZ17I   Bsu36I    CspCI     DraI
     EagI     FseI    FspAI     HpaI     MfeI     MluI     NotI     NruI
     NsiI     PacI     PmeI     PpiI    PshAI     PsiI   PspOMI     PvuI
    RsrII     SbfI     SgfI    SgrAI     SrfI     SwaI     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com