Restriction map for pF1KB3232
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BciVI       BclI        BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrFI       BsrGI       BssHII      BstAPI      BstBI       
Cfr10I      ClaI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KspI        MaeII       MamI        MfeI        MluI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        SacII       
SalI        SapI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccI          120  GTATAC               GT^[AC][GT]AC
AccIII       1200  TCCGGA               T^CCGGA
AcsI         2133  AAATTC               [GA]^AATT[CT]
AflII         859  CTTAAG               C^TTAAG
ApoI         2133  AAATTC               [GA]^AATT[CT]
AspI         1120  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI         489  CCTCAGC              CCTCAGC(-5/-2)
BcgI          903  CGACAAGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          859  CTTAAG               C^TTAAG
BlpI          357  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      357  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1200  TCCGGA               [AT]^CCGG[AT]
BseAI        1200  TCCGGA               T^CCGGA
BspEI        1200  TCCGGA               T^CCGGA
BspHI         581  TCATGA               T^CATGA
BssSI          10  CTCGTG               CACGAG(-5/-1)
Bst1107I      120  GTATAC               GTA^TAC
BstEII       1635  GGTCACC              G^GT[ACGT]ACC
BstPI        1635  GGTCACC              G^GT[ACGT]ACC
BstXI        1706  CCACAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       120  GTATAC               GTA^TAC
CelII         357  GCTGAGC              GC^T[ACGT]AGC
CpoI         1220  CGGTCCG              CG^G[AT]CCG
DraIII       1182  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1059  GACGGTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1468  GAAGAG               CTCTTC(1/4)
EcoO65I      1635  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1947  CCTTGG               C^C[AT][AT]GG
HaeII        1211  AGCGCC               [GA]GCGC^[CT]
HindIII      1281  AAGCTT               A^AGCTT
Ksp632I      1468  GAAGAG               CTCTTC(1/4)
MboII        1468  GAAGA                GAAGA(8/7)
MroI         1200  TCCGGA               T^CCGGA
MseI          860  TTAA                 T^TAA
MslI         1485  CACCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI        1120  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1497  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1066  GAGTC                GAGTC(4/5)
PpuMI        1438  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI         180  GACGGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          581  TCATGA               T^CATGA
RsrII        1220  CGGTCCG              CG^G[AT]CCG
SacI         1588  GAGCTC               GAGCT^C
SbfI         1080  CCTGCAGG             CCTGCA^GG
ScaI         1775  AGTACT               AGT^ACT
Sse8387I     1080  CCTGCAGG             CCTGCA^GG
StyI         1947  CCTTGG               C^C[AT][AT]GG
TfiI         2070  GAATC                G^A[AT]TC
Tru9I         860  TTAA                 T^TAA
Tsp509I      2134  AATT                 ^AATT
Tth111I      1120  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1497  CCAGAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Acc65I         66  GGTACC               G^GTACC
             1418  GGTACC              
AccII         142  CGCG                 CG^CG
              985  CGCG                
AocI          528  CCTGAGG              CC^T[ACGT]AGG
             1871  CCTTAGG             
Asp718I        66  GGTACC               G^GTACC
             1418  GGTACC              
AspHI        1547  GTGCTC               G[AT]GC[AT]^C
             1588  GAGCTC              
BglI          469  GCCCCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1897  GCCATCCTGGC         
BglII         680  AGATCT               A^GATCT
             1361  AGATCT              
BsiHKAI      1547  GTGCTC               G[AT]GC[AT]^C
             1588  GAGCTC              
BsrBI         129  CCGCTC               CCGCTC(-3/-3)
              814  CCGCTC              
BstUI         142  CGCG                 CG^CG
              985  CGCG                
Bsu36I        528  CCTGAGG              CC^T[ACGT]AGG
             1871  CCTTAGG             
CfoI          986  GCGC                 GCG^C
             1212  GCGC                
DraII         475  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1438  GGGACCC             
Eco81I        528  CCTGAGG              CC^T[ACGT]AGG
             1871  CCTTAGG             
EcoNI        1080  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1758  CCTCCTTCAGG         
EcoO109I      475  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1438  GGGACCC             
HhaI          986  GCGC                 GCG^C
             1212  GCGC                
HinP1I        986  GCGC                 G^CGC
             1212  GCGC                
HinfI        1066  GAGTC                G^A[ACGT]TC
             2070  GAATC               
KpnI           66  GGTACC               GGTAC^C
             1418  GGTACC              
MvnI          142  CGCG                 CG^CG
              985  CGCG                
NspI           75  GCATGT               [GA]CATG^[CT]
              848  ACATGC              
PstI          226  CTGCAG               CTGCA^G
             1081  CTGCAG              
SexAI        1502  ACCTGGT              A^CC[AT]GGT
             1669  ACCTGGT             
SmaI          749  CCCGGG               CCC^GGG
              781  CCCGGG              
SmlI          787  CTCAAG               C^T[CT][GA]AG
              859  CTTAAG              
XmaCI         749  CCCGGG               C^CCGGG
              781  CCCGGG              
XmaI          749  CCCGGG               C^CCGGG
              781  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NheI        NlaIII      NlaIV       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XcmI        XhoII       
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