Restriction map for pF1KB3232
 (Linear) (Six-base) MAPSORT of: KIBB3232.seq  Check: 5366  from: 1  to: 2139



KIBB3232.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1007    1407    2139 
   Size:       1007     400     732

  Fragments arranged by size: 

                1007     732     400
 
AccI GT'mk_AC

Cuts at:      0     121    2139 
   Size:        121    2018
 
Acc65I G'GTAC_C

Cuts at:      0      66    1418    2139 
   Size:         66    1352     721

  Fragments arranged by size: 

                1352     721      66
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1747    2139 
   Size:       1747     392
 
AflII C'TTAA_G

Cuts at:      0     859    2139 
   Size:        859    1280
 
AleI CACnn'nnGTG

Cuts at:      0    1489    2139 
   Size:       1489     650
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     517     688    1342    2139 
   Size:        517     171     654     797

  Fragments arranged by size: 

                 797     654     517     171
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219     357     548    1503    1584    2139 
   Size:        219     138     191     955      81     555

  Fragments arranged by size: 

                 955     555     219     191     138      81
 
ApoI r'AATT_y

Cuts at:      0    2133    2139 
   Size:       2133       6
 
AvaI C'yCGr_G

Cuts at:      0     323     749     781     928    1357    1432    1727    2139 
   Size:        323     426      32     147     429      75     295     412

  Fragments arranged by size: 

                 429     426     412     323     295     147      75      32
            
 
BanI G'GyrC_C

Cuts at:      0      43      66     753     777    1150    1230    1418    2139 
   Size:         43      23     687      24     373      80     188     721

  Fragments arranged by size: 

                 721     687     373     188      80      43      24      23
            
 
BanII G_rGCy'C

Cuts at:      0     788    1592    1892    2139 
   Size:        788     804     300     247

  Fragments arranged by size: 

                 804     788     300     247
 
BbvCI CC'TCA_GC

Cuts at:      0     490    2139 
   Size:        490    1649
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     892     926    2139 
   Size:        892      34    1213

  Fragments arranged by size: 

                1213     892      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     475    1903    2139 
   Size:        475    1428     236

  Fragments arranged by size: 

                1428     475     236
 
BglII A'GATC_T

Cuts at:      0     680    1361    2139 
   Size:        680     681     778

  Fragments arranged by size: 

                 778     681     680
 
BlpI GC'TnA_GC

Cuts at:      0     358    2139 
   Size:        358    1781
 
Bme1580I G_kGCm'C

Cuts at:      0      62     758     768     782    1153    1235    1341    2028 
   Size:         62     696      10      14     371      82     106     687

Cuts at:   2028    2139 
   Size:        111

  Fragments arranged by size: 

                 696     687     371     111     106      82      62      14
                  10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1377    1788    1812    2139 
   Size:       1377     411      24     327

  Fragments arranged by size: 

                1377     411     327      24
 
BmtI G_CTAG'C

Cuts at:      0      31     661    1939    2139 
   Size:         31     630    1278     200

  Fragments arranged by size: 

                1278     630     200      31
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     741     912    2006    2139 
   Size:        741     171    1094     133

  Fragments arranged by size: 

                1094     741     171     133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     718     736     802    1192    1282    1361    1465 
   Size:        415     303      18      66     390      90      79     104

Cuts at:   1465    1540    1606    1982    2040    2139 
   Size:         75      66     376      58      99

  Fragments arranged by size: 

                 415     390     376     303     104      99      90      79
                  75      66      66      58      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     490    2139 
   Size:        490    1649
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    2139 
   Size:        772    1367
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     900    1006    1099    1597    2139 
   Size:        900     106      93     498     542

  Fragments arranged by size: 

                 900     542     498     106      93
 
BsaWI w'CCGG_w

Cuts at:      0    1200    2139 
   Size:       1200     939
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     502     532    2098    2128    2139 
   Size:        502      30    1566      30      11

  Fragments arranged by size: 

                1566     502      30      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     388     901    1543    1674    2139 
   Size:        388     513     642     131     465

  Fragments arranged by size: 

                 642     513     465     388     131
 
BseYI C'CCAG_C

Cuts at:      0     264     350     867    1133    1381    1930    2139 
   Size:        264      86     517     266     248     549     209

  Fragments arranged by size: 

                 549     517     266     264     248     209      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     493     838    1618    2139 
   Size:        349     144     345     780     521

  Fragments arranged by size: 

                 780     521     349     345     144
 
BsiHKAI G_wGCw'C

Cuts at:      0    1551    1592    2139 
   Size:       1551      41     547

  Fragments arranged by size: 

                1551     547      41
 
Bsp1286I G_dGCh'C

Cuts at:      0      62     758     768     782     788    1153    1235    1341 
   Size:         62     696      10      14       6     365      82     106

Cuts at:   1341    1551    1592    1892    2028    2139 
   Size:        210      41     300     136     111

  Fragments arranged by size: 

                 696     365     300     210     136     111     106      82
                  62      41      14      10       6
 
BspEI T'CCGG_A

Cuts at:      0    1200    2139 
   Size:       1200     939
 
BspHI T'CATG_A

Cuts at:      0     581    2139 
   Size:        581    1558
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     650     680    1007    1088    1407    2139 
   Size:        650      30     327      81     319     732

  Fragments arranged by size: 

                 732     650     327     319      81      30
 
BsrBI CCG'CTC

Cuts at:      0     131     816    2139 
   Size:        131     685    1323

  Fragments arranged by size: 

                1323     685     131
 
BsrDI GCAATG_nn'

Cuts at:      0     171    1329    1892    2139 
   Size:        171    1158     563     247

  Fragments arranged by size: 

                1158     563     247     171
 
BssSI C'ACGA_G

Cuts at:      0      10    2139 
   Size:         10    2129
 
BstEII G'GTnAC_C

Cuts at:      0    1635    2139 
   Size:       1635     504
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1713    2139 
   Size:       1713     426
 
BstYI r'GATC_y

Cuts at:      0     107     680    1361    2092    2139 
   Size:        107     573     681     731      47

  Fragments arranged by size: 

                 731     681     573     107      47
 
BstZ17I GTA'TAC

Cuts at:      0     122    2139 
   Size:        122    2017
 
Bsu36I CC'TnA_GG

Cuts at:      0     529    1872    2139 
   Size:        529    1343     267

  Fragments arranged by size: 

                1343     529     267
 
BtgI C'CryG_G

Cuts at:      0      62     961    1098    1145    1769    2139 
   Size:         62     899     137      47     624     370

  Fragments arranged by size: 

                 899     624     370     137      62      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     450    2139 
   Size:        450    1689
 
BtsI GCAGTG_nn'

Cuts at:      0      47    2139 
   Size:         47    2092
 
DraIII CAC_nnn'GTG

Cuts at:      0    1187    2139 
   Size:       1187     952
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1065    2139 
   Size:       1065    1074
 
EaeI y'GGCC_r

Cuts at:      0     275     419     635    1343    2139 
   Size:        275     144     216     708     796

  Fragments arranged by size: 

                 796     708     275     216     144
 
EarI CTCTTCn'nnn_

Cuts at:      0    1463    2139 
   Size:       1463     676
 
EcoICRI GAG'CTC

Cuts at:      0    1590    2139 
   Size:       1590     549
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     718     736     802    1192    1282    1361    1465 
   Size:        415     303      18      66     390      90      79     104

Cuts at:   1465    1540    1606    1747    1982    2040    2139 
   Size:         75      66     141     235      58      99

  Fragments arranged by size: 

                 415     390     303     235     141     104      99      90
                  79      75      66      66      58      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1084    1762    2139 
   Size:       1084     678     377

  Fragments arranged by size: 

                1084     678     377
 
EcoO109I rG'GnC_Cy

Cuts at:      0     476    1439    2139 
   Size:        476     963     700

  Fragments arranged by size: 

                 963     700     476
 
HaeII r_GCGC'y

Cuts at:      0    1215    2139 
   Size:       1215     924
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1162    1194    2139 
   Size:       1162      32     945

  Fragments arranged by size: 

                1162     945      32
 
HindIII A'AGCT_T

Cuts at:      0    1281    2139 
   Size:       1281     858
 
KpnI G_GTAC'C

Cuts at:      0      70    1422    2139 
   Size:         70    1352     717

  Fragments arranged by size: 

                1352     717      70
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     710    2139 
   Size:        545     165    1429

  Fragments arranged by size: 

                1429     545     165
 
MscI TGG'CCA

Cuts at:      0     277     421     637    2139 
   Size:        277     144     216    1502

  Fragments arranged by size: 

                1502     277     216     144
 
MslI CAynn'nnrTG

Cuts at:      0    1489    2139 
   Size:       1489     650
 
MspA1I CmG'CkG

Cuts at:      0     354     384     548     744     822    1115    1133    1381 
   Size:        354      30     164     196      78     293      18     248

Cuts at:   1381    1930    2139 
   Size:        549     209

  Fragments arranged by size: 

                 549     354     293     248     209     196     164      78
                  30      18
 
NheI G'CTAG_C

Cuts at:      0      27     657    1935    2139 
   Size:         27     630    1278     204

  Fragments arranged by size: 

                1278     630     204      27
 
NspI r_CATG'y

Cuts at:      0      79     852    2139 
   Size:         79     773    1287

  Fragments arranged by size: 

                1287     773      79
 
PasI CC'CwG_GG

Cuts at:      0     473    2139 
   Size:        473    1666
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1503    2139 
   Size:       1503     636
 
PfoI T'CCnGG_A

Cuts at:      0     611    1238    2139 
   Size:        611     627     901

  Fragments arranged by size: 

                 901     627     611
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     425     457    2139 
   Size:        425      32    1682

  Fragments arranged by size: 

                1682     425      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1439    2139 
   Size:       1439     700
 
PshAI GACnn'nnGTC

Cuts at:      0     184    2139 
   Size:        184    1955
 
PstI C_TGCA'G

Cuts at:      0     230    1085    2139 
   Size:        230     855    1054

  Fragments arranged by size: 

                1054     855     230
 
PvuII CAG'CTG

Cuts at:      0     354     384     744    1381    1930    2139 
   Size:        354      30     360     637     549     209

  Fragments arranged by size: 

                 637     549     360     354     209      30
 
RsrII CG'GwC_CG

Cuts at:      0    1221    2139 
   Size:       1221     918
 
SacI G_AGCT'C

Cuts at:      0    1592    2139 
   Size:       1592     547
 
SanDI GG'GwC_CC

Cuts at:      0    1439    2139 
   Size:       1439     700
 
SbfI CC_TGCA'GG

Cuts at:      0    1085    2139 
   Size:       1085    1054
 
ScaI AGT'ACT

Cuts at:      0    1777    2139 
   Size:       1777     362
 
SexAI A'CCwGG_T

Cuts at:      0    1502    1669    2139 
   Size:       1502     167     470

  Fragments arranged by size: 

                1502     470     167
 
SfcI C'TryA_G

Cuts at:      0     226     427    1081    2139 
   Size:        226     201     654    1058

  Fragments arranged by size: 

                1058     654     226     201
 
SmaI CCC'GGG

Cuts at:      0     751     783    2139 
   Size:        751      32    1356

  Fragments arranged by size: 

                1356     751      32
 
SmlI C'TyrA_G

Cuts at:      0     787     859    2139 
   Size:        787      72    1280

  Fragments arranged by size: 

                1280     787      72
 
SrfI GCCC'GGGC

Cuts at:      0     783    2139 
   Size:        783    1356
 
StyI C'CwwG_G

Cuts at:      0    1947    2139 
   Size:       1947     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      24    1659    1831    1966    2139 
   Size:         24    1635     172     135     173

  Fragments arranged by size: 

                1635     173     172     135      24
 
TatI w'GTAC_w

Cuts at:      0    1775    2139 
   Size:       1775     364
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     502     534    1268    1300    2139 
   Size:        502      32     734      32     839

  Fragments arranged by size: 

                 839     734     502      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1123    2139 
   Size:       1123    1016
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     765    1306    1971    2139 
   Size:         60     705     541     665     168

  Fragments arranged by size: 

                 705     665     541     168      60
 
XmaI C'CCGG_G

Cuts at:      0     749     781    2139 
   Size:        749      32    1358

  Fragments arranged by size: 

                1358     749      32
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII     AleI     AlfI    AlwNI
     ApoI     AvaI     BanI    BanII    BbvCI     BcgI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BssSI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NheI     NspI
     PasI    PflMI     PfoI     PpiI    PpuMI    PshAI     PstI    PvuII
    RsrII     SacI    SanDI     SbfI     ScaI    SexAI     SfcI     SmaI
     SmlI     SrfI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI   AflIII     AgeI     AhdI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BciVI     BclI    BfrBI    BmgBI    BsaAI    BsaBI    BsaHI
    BsiEI    BsiWI     BsmI    BsmBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI     ClaI    CspCI     DraI     EagI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PsiI   PspOMI    PspXI
     PsrI     PvuI    SacII     SalI     SapI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     XbaI
     XhoI     XmnI     ZraI
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