Restriction map for pF1KB3039
 (Linear) (Six-base) MAPSORT of: KIBB3039.seq  Check: 3088  from: 1  to: 3372



KIBB3039.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     136     539    3372 
   Size:        136     403    2833

  Fragments arranged by size: 

                2833     403     136
 
AflII C'TTAA_G

Cuts at:      0    2515    3372 
   Size:       2515     857
 
AflIII A'CryG_T

Cuts at:      0     748     773    3372 
   Size:        748      25    2599

  Fragments arranged by size: 

                2599     748      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1288    3372 
   Size:       1288    2084
 
ApoI r'AATT_y

Cuts at:      0    1093    1677    2473    3372 
   Size:       1093     584     796     899

  Fragments arranged by size: 

                1093     899     796     584
 
AvaI C'yCGr_G

Cuts at:      0    2846    2957    3372 
   Size:       2846     111     415

  Fragments arranged by size: 

                2846     415     111
 
BamHI G'GATC_C

Cuts at:      0     207    1238    3372 
   Size:        207    1031    2134

  Fragments arranged by size: 

                2134    1031     207
 
BanI G'GyrC_C

Cuts at:      0      64     146    3372 
   Size:         64      82    3226

  Fragments arranged by size: 

                3226      82      64
 
BanII G_rGCy'C

Cuts at:      0     161    1013    3372 
   Size:        161     852    2359

  Fragments arranged by size: 

                2359     852     161
 
BbeI G_GCGC'C

Cuts at:      0     150    3372 
   Size:        150    3222
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2048    3372 
   Size:       2048    1324
 
BbvCI CC'TCA_GC

Cuts at:      0    1959    3372 
   Size:       1959    1413
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     329    3372 
   Size:        329    3043
 
BclI T'GATC_A

Cuts at:      0      83    1758    2300    2592    3372 
   Size:         83    1675     542     292     780

  Fragments arranged by size: 

                1675     780     542     292      83
 
BfrBI ATG'CAT

Cuts at:      0     719    3372 
   Size:        719    2653
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2842    3372 
   Size:       2842     530
 
BglII A'GATC_T

Cuts at:      0    1881    3372 
   Size:       1881    1491
 
BlpI GC'TnA_GC

Cuts at:      0     542    3372 
   Size:        542    2830
 
Bme1580I G_kGCm'C

Cuts at:      0     497    3372 
   Size:        497    2875
 
BmgBI CAC'GTC

Cuts at:      0     749    2841    3372 
   Size:        749    2092     531

  Fragments arranged by size: 

                2092     749     531
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2995    3372 
   Size:       2995     377
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     607    3372 
   Size:        607    2765
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     731     980     997    2779    3190    3372 
   Size:        578     153     249      17    1782     411     182

  Fragments arranged by size: 

                1782     578     411     249     182     153      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     937    1959    3099    3372 
   Size:        937    1022    1140     273

  Fragments arranged by size: 

                1140    1022     937     273
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    2080    2189    2883    3372 
   Size:        209    1871     109     694     489

  Fragments arranged by size: 

                1871     694     489     209     109
 
BsaAI yAC'GTr

Cuts at:      0    1833    3306    3372 
   Size:       1833    1473      66

  Fragments arranged by size: 

                1833    1473      66
 
BsaBI GATnn'nnATC

Cuts at:      0      82    3016    3372 
   Size:         82    2934     356

  Fragments arranged by size: 

                2934     356      82
 
BsaHI Gr'CG_yC

Cuts at:      0     147    3372 
   Size:        147    3225
 
BsaWI w'CCGG_w

Cuts at:      0     623    1235    3372 
   Size:        623     612    2137

  Fragments arranged by size: 

                2137     623     612
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     398     428    1250    1280    1726    1756    3372 
   Size:        398      30     822      30     446      30    1616

  Fragments arranged by size: 

                1616     822     446     398      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     335    1622    1907    1947    3304    3372 
   Size:        335    1287     285      40    1357      68

  Fragments arranged by size: 

                1357    1287     335     285      68      40
 
BseYI C'CCAG_C

Cuts at:      0    1288    2166    2769    2813    3372 
   Size:       1288     878     603      44     559

  Fragments arranged by size: 

                1288     878     603     559      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     754     822     963    2401    3152    3372 
   Size:        754      68     141    1438     751     220

  Fragments arranged by size: 

                1438     754     751     220     141      68
 
BsiHKAI G_wGCw'C

Cuts at:      0    1178    3372 
   Size:       1178    2194
 
BsmI GAATG_Cn'

Cuts at:      0     661     721    1350    2076    3372 
   Size:        661      60     629     726    1296

  Fragments arranged by size: 

                1296     726     661     629      60
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1593    2755    3372 
   Size:       1593    1162     617

  Fragments arranged by size: 

                1593    1162     617
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     497    1013    1178    3372 
   Size:        161     336     516     165    2194

  Fragments arranged by size: 

                2194     516     336     165     161
 
BspEI T'CCGG_A

Cuts at:      0     623    3372 
   Size:        623    2749
 
BspHI T'CATG_A

Cuts at:      0    2178    3372 
   Size:       2178    1194
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136     539    2281    3372 
   Size:        136     403    1742    1091

  Fragments arranged by size: 

                1742    1091     403     136
 
BsrDI GCAATG_nn'

Cuts at:      0     131     900    2780    3372 
   Size:        131     769    1880     592

  Fragments arranged by size: 

                1880     769     592     131
 
BsrGI T'GTAC_A

Cuts at:      0    3041    3372 
   Size:       3041     331
 
BssHII G'CGCG_C

Cuts at:      0    2521    3372 
   Size:       2521     851
 
BstEII G'GTnAC_C

Cuts at:      0    2187    3372 
   Size:       2187    1185
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     556    3008    3372 
   Size:        556    2452     364

  Fragments arranged by size: 

                2452     556     364
 
BstYI r'GATC_y

Cuts at:      0     207    1238    1881    2433    2889    3372 
   Size:        207    1031     643     552     456     483

  Fragments arranged by size: 

                1031     643     552     483     456     207
 
BtgI C'CryG_G

Cuts at:      0      68    2718    3372 
   Size:         68    2650     654

  Fragments arranged by size: 

                2650     654      68
 
BtsI GCAGTG_nn'

Cuts at:      0     464     810     895    1278    1886    3372 
   Size:        464     346      85     383     608    1486

  Fragments arranged by size: 

                1486     608     464     383     346      85
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1037    1072    3372 
   Size:       1037      35    2300

  Fragments arranged by size: 

                2300    1037      35
 
EaeI y'GGCC_r

Cuts at:      0     569    1123    2505    2699    3014    3372 
   Size:        569     554    1382     194     315     358

  Fragments arranged by size: 

                1382     569     554     358     315     194
 
EarI CTCTTCn'nnn_

Cuts at:      0     259     598    2311    3372 
   Size:        259     339    1713    1061

  Fragments arranged by size: 

                1713    1061     339     259
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     731     980     997    2779    3190    3372 
   Size:        578     153     249      17    1782     411     182

  Fragments arranged by size: 

                1782     578     411     249     182     153      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     609    1253    1637    3372 
   Size:        609     644     384    1735

  Fragments arranged by size: 

                1735     644     609     384
 
EcoO109I rG'GnC_Cy

Cuts at:      0     649    2100    3372 
   Size:        649    1451    1272

  Fragments arranged by size: 

                1451    1272     649
 
HaeII r_GCGC'y

Cuts at:      0     150    3372 
   Size:        150    3222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     314     346     954     986    1195    1227    1250    1282 
   Size:        314      32     608      32     209      32      23      32

Cuts at:   1282    1726    1758    1832    1864    1866    1898    3049    3081 
   Size:        444      32      74      32       2      32    1151      32

Cuts at:   3081    3232    3264    3372 
   Size:        151      32     108

  Fragments arranged by size: 

                1151     608     444     314     209     151     108      74
                  32      32      32      32      32      32      32      32
                  32      23       2
 
HincII GTy'rAC

Cuts at:      0     358    1851    3372 
   Size:        358    1493    1521

  Fragments arranged by size: 

                1521    1493     358
 
HindIII A'AGCT_T

Cuts at:      0     362     934    1112    1438    3372 
   Size:        362     572     178     326    1934

  Fragments arranged by size: 

                1934     572     362     326     178
 
KasI G'GCGC_C

Cuts at:      0     146    3372 
   Size:        146    3226
 
MfeI C'AATT_G

Cuts at:      0    2311    3372 
   Size:       2311    1061
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1967    3372 
   Size:       1967    1405
 
MscI TGG'CCA

Cuts at:      0    2507    2701    3016    3372 
   Size:       2507     194     315     356

  Fragments arranged by size: 

                2507     356     315     194
 
MslI CAynn'nnrTG

Cuts at:      0     349    2054    3372 
   Size:        349    1705    1318

  Fragments arranged by size: 

                1705    1318     349
 
MspA1I CmG'CkG

Cuts at:      0     213     947    1963    2215    2377    3372 
   Size:        213     734    1016     252     162     995

  Fragments arranged by size: 

                1016     995     734     252     213     162
 
NarI GG'CG_CC

Cuts at:      0     147    3372 
   Size:        147    3225
 
NcoI C'CATG_G

Cuts at:      0      68    2718    3372 
   Size:         68    2650     654

  Fragments arranged by size: 

                2650     654      68
 
NsiI A_TGCA'T

Cuts at:      0     721    3372 
   Size:        721    2651
 
NspI r_CATG'y

Cuts at:      0     905    2675    3372 
   Size:        905    1770     697

  Fragments arranged by size: 

                1770     905     697
 
PasI CC'CwG_GG

Cuts at:      0    1820    1863    3156    3372 
   Size:       1820      43    1293     216

  Fragments arranged by size: 

                1820    1293     216      43
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     242     875    3264    3372 
   Size:        242     633    2389     108

  Fragments arranged by size: 

                2389     633     242     108
 
PfoI T'CCnGG_A

Cuts at:      0     996    1587    3372 
   Size:        996     591    1785

  Fragments arranged by size: 

                1785     996     591
 
PpuMI rG'GwC_Cy

Cuts at:      0     649    3372 
   Size:        649    2723
 
PstI C_TGCA'G

Cuts at:      0     890    1273    3372 
   Size:        890     383    2099

  Fragments arranged by size: 

                2099     890     383
 
PvuII CAG'CTG

Cuts at:      0     947    1963    2377    3372 
   Size:        947    1016     414     995

  Fragments arranged by size: 

                1016     995     947     414
 
SexAI A'CCwGG_T

Cuts at:      0    2123    3372 
   Size:       2123    1249
 
SfcI C'TryA_G

Cuts at:      0     886    1269    3162    3372 
   Size:        886     383    1893     210

  Fragments arranged by size: 

                1893     886     383     210
 
SfoI GGC'GCC

Cuts at:      0     148    3372 
   Size:        148    3224
 
SmaI CCC'GGG

Cuts at:      0    2848    3372 
   Size:       2848     524
 
SmlI C'TyrA_G

Cuts at:      0     224    2095    2204    2515    2862    3372 
   Size:        224    1871     109     311     347     510

  Fragments arranged by size: 

                1871     510     347     311     224     109
 
SphI G_CATG'C

Cuts at:      0     905    3372 
   Size:        905    2467
 
SspI AAT'ATT

Cuts at:      0    1697    1770    1982    3372 
   Size:       1697      73     212    1390

  Fragments arranged by size: 

                1697    1390     212      73
 
StyI C'CwwG_G

Cuts at:      0      68     978    1856    2253    2718    3372 
   Size:         68     910     878     397     465     654

  Fragments arranged by size: 

                 910     878     654     465     397      68
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     131     845    3372 
   Size:        131     714    2527

  Fragments arranged by size: 

                2527     714     131
 
TatI w'GTAC_w

Cuts at:      0     525     563     670    3041    3372 
   Size:        525      38     107    2371     331

  Fragments arranged by size: 

                2371     525     331     107      38
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     398     430    1887    1919    3114    3146    3372 
   Size:        398      32    1457      32    1195      32     226

  Fragments arranged by size: 

                1457    1195     398     226      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     519    3372 
   Size:        519    2853
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     706    2456    3372 
   Size:        706    1750     916

  Fragments arranged by size: 

                1750     916     706
 
XmaI C'CCGG_G

Cuts at:      0    2846    3372 
   Size:       2846     526
 

 Enzymes that do cut:

     AarI    AflII   AflIII    AlwNI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrGI   BssHII   BstEII    BstXI    BstYI     BtgI     BtsI
    CspCI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
   HincII  HindIII     KasI     MfeI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NsiI     NspI     PasI    PflMI     PfoI    PpuMI
     PstI    PvuII    SexAI     SfcI     SfoI     SmaI     SmlI     SphI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BcgI     BmtI     BsaI    BsiEI    BsiWI    BsrBI
    BsrFI    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KpnI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     StuI     SwaI
  Tth111I     XhoI     XmnI     ZraI
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