(Circular) (Six-base) MAPSORT of: pF1KB3020.seq Check: 4970 from: 1 to: 5036 pF1KB3020 5036 bp With 182 enzymes: SgfI * September 18, 2009 17:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1158 1398 1158 Size: 240 4796 AccI GT'mk_AC Cuts at: 2074 2074 Size: 5036 Acc65I G'GTAC_C Cuts at: 600 2052 600 Size: 1452 3584 AclI AA'CG_TT Cuts at: 4876 4876 Size: 5036 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1145 1585 1711 2042 2861 3293 4040 4308 1145 Size: 440 126 331 819 432 747 268 1873 Fragments arranged by size: 1873 819 747 440 432 331 268 126 AflIII A'CryG_T Cuts at: 1925 2388 3492 4469 1925 Size: 463 1104 977 2492 Fragments arranged by size: 2492 1104 977 463 AgeI A'CCGG_T Cuts at: 3424 3424 Size: 5036 AhdI GACnn_n'nnGTC Cuts at: 1299 1299 Size: 5036 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 199 505 199 Size: 306 4730 AlwNI CAG_nnn'CTG Cuts at: 597 1604 2341 3908 597 Size: 1007 737 1567 1725 Fragments arranged by size: 1725 1567 1007 737 ApaI G_GGCC'C Cuts at: 1586 1873 1891 1586 Size: 287 18 4731 Fragments arranged by size: 4731 287 18 ApaLI G'TGCA_C Cuts at: 1411 3806 1411 Size: 2395 2641 ApoI r'AATT_y Cuts at: 2040 4389 4645 2040 Size: 2349 256 2431 Fragments arranged by size: 2431 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5036 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5036 AvaI C'yCGr_G Cuts at: 746 1466 1864 1936 2056 746 Size: 720 398 72 120 3726 Fragments arranged by size: 3726 720 398 120 72 BamHI G'GATC_C Cuts at: 1638 2061 1638 Size: 423 4613 BanI G'GyrC_C Cuts at: 600 976 1509 2052 2466 2714 2749 600 Size: 376 533 543 414 248 35 2887 Fragments arranged by size: 2887 543 533 414 376 248 35 BanII G_rGCy'C Cuts at: 1586 1654 1863 1873 1891 2050 3080 1586 Size: 68 209 10 18 159 1030 3542 Fragments arranged by size: 3542 1030 209 159 68 18 10 BbeI G_GCGC'C Cuts at: 2470 2718 2470 Size: 248 4788 BbsI GAAGACnn'nnnn_ Cuts at: 1133 1508 1699 1133 Size: 375 191 4470 Fragments arranged by size: 4470 375 191 BbvCI CC'TCA_GC Cuts at: 1916 1916 Size: 5036 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 693 727 693 Size: 34 5002 BciVI GTATCCnnnnn_n' Cuts at: 2934 3695 5036 2934 Size: 761 1341 2934 Fragments arranged by size: 2934 1341 761 BclI T'GATC_A Cuts at: 1383 2556 1383 Size: 1173 3863 BglI GCCn_nnn'nGGC Cuts at: 143 283 2016 143 Size: 140 1733 3163 Fragments arranged by size: 3163 1733 140 BglII A'GATC_T Cuts at: 557 1181 1193 2551 4588 557 Size: 624 12 1358 2037 1005 Fragments arranged by size: 2037 1358 1005 624 12 BlpI GC'TnA_GC Cuts at: 2148 2148 Size: 5036 Bme1580I G_kGCm'C Cuts at: 882 1415 1514 1586 1873 1891 2661 2754 3810 Size: 533 99 72 287 18 770 93 1056 Cuts at: 3810 882 Size: 2108 Fragments arranged by size: 2108 1056 770 533 287 99 93 72 18 BmgBI CAC'GTC Cuts at: 234 234 Size: 5036 BmrI ACTGGGnnnn_n' Cuts at: 318 857 2248 2329 2664 4808 318 Size: 539 1391 81 335 2144 546 Fragments arranged by size: 2144 1391 546 539 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3403 3403 Size: 5036 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 439 1048 1359 439 Size: 609 311 4116 Fragments arranged by size: 4116 609 311 Bpu10I CC'TnA_GC Cuts at: 1916 1916 Size: 5036 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2212 3091 3583 3881 2212 Size: 879 492 298 3367 Fragments arranged by size: 3367 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1463 4740 1463 Size: 3277 1759 BsaAI yAC'GTr Cuts at: 2389 2389 Size: 5036 BsaBI GATnn'nnATC Cuts at: 6 1208 2550 4587 6 Size: 1202 1342 2037 455 Fragments arranged by size: 2037 1342 1202 455 BsaHI Gr'CG_yC Cuts at: 1696 2467 2715 3417 4913 1696 Size: 771 248 702 1496 1819 Fragments arranged by size: 1819 1496 771 702 248 BsaWI w'CCGG_w Cuts at: 2225 2746 3424 3698 3845 4256 2225 Size: 521 678 274 147 411 3005 Fragments arranged by size: 3005 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1765 1795 1765 Size: 30 5006 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 748 751 1079 1164 1904 1924 748 Size: 3 328 85 740 20 3860 Fragments arranged by size: 3860 740 328 85 20 3 BseYI C'CCAG_C Cuts at: 264 1043 1068 1098 1585 2461 3219 3796 4292 Size: 779 25 30 487 876 758 577 496 Cuts at: 4292 264 Size: 1008 Fragments arranged by size: 1008 876 779 758 577 496 487 30 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 172 779 172 Size: 607 4429 BsiEI CG_ry'CG Cuts at: 104 524 2578 2624 3832 104 Size: 420 2054 46 1208 1308 Fragments arranged by size: 2054 1308 1208 420 46 BsiHKAI G_wGCw'C Cuts at: 239 1415 1654 2050 2828 3018 3810 239 Size: 1176 239 396 778 190 792 1465 Fragments arranged by size: 1465 1176 792 778 396 239 190 Bsp1286I G_dGCh'C Cuts at: 239 882 1415 1514 1586 1654 1863 1873 1891 Size: 643 533 99 72 68 209 10 18 Cuts at: 1891 2050 2661 2754 2828 3018 3080 3810 239 Size: 159 611 93 74 190 62 730 1465 Fragments arranged by size: 1465 730 643 611 533 209 190 159 99 93 74 72 68 62 18 10 BspEI T'CCGG_A Cuts at: 4256 4256 Size: 5036 BspHI T'CATG_A Cuts at: 515 1497 4212 515 Size: 982 2715 1339 Fragments arranged by size: 2715 1339 982 BspMI ACCTGCnnnn'nnnn_ Cuts at: 938 1158 1398 1453 2086 2300 2602 2983 938 Size: 220 240 55 633 214 302 381 2991 Fragments arranged by size: 2991 633 381 302 240 220 214 55 BsrBI CCG'CTC Cuts at: 50 114 1425 1814 3328 3382 4866 5031 50 Size: 64 1311 389 1514 54 1484 165 55 Fragments arranged by size: 1514 1484 1311 389 165 64 55 54 BsrDI GCAATG_nn' Cuts at: 711 764 1455 2948 711 Size: 53 691 1493 2799 Fragments arranged by size: 2799 1493 691 53 BsrFI r'CCGG_y Cuts at: 715 1022 1220 3034 3215 3424 715 Size: 307 198 1814 181 209 2327 Fragments arranged by size: 2327 1814 307 209 198 181 BssSI C'ACGA_G Cuts at: 1036 3307 3665 1036 Size: 2271 358 2407 Fragments arranged by size: 2407 2271 358 BstAPI GCAn_nnn'nTGC Cuts at: 766 877 1595 2413 766 Size: 111 718 818 3389 Fragments arranged by size: 3389 818 718 111 BstBI TT'CG_AA Cuts at: 3397 3397 Size: 5036 BstEII G'GTnAC_C Cuts at: 1666 1666 Size: 5036 BstXI CCAn_nnnn'nTGG Cuts at: 933 1360 1525 933 Size: 427 165 4444 Fragments arranged by size: 4444 427 165 BstYI r'GATC_y Cuts at: 1 557 593 1181 1193 1638 1989 2061 2528 Size: 556 36 588 12 445 351 72 467 Cuts at: 2528 2551 2886 3132 4144 4230 4242 4588 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 588 556 467 449 445 351 346 335 246 86 72 36 23 12 12 Bsu36I CC'TnA_GG Cuts at: 964 1994 964 Size: 1030 4006 BtgI C'CryG_G Cuts at: 107 815 1331 2012 107 Size: 708 516 681 3131 Fragments arranged by size: 3131 708 681 516 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 696 2986 3167 3330 696 Size: 2290 181 163 2402 Fragments arranged by size: 2402 2290 181 163 BtsI GCAGTG_nn' Cuts at: 2279 2279 Size: 5036 DraI TTT'AAA Cuts at: 2035 2035 Size: 5036 DrdI GACnn_nn'nnGTC Cuts at: 1741 2742 3600 1741 Size: 1001 858 3177 Fragments arranged by size: 3177 1001 858 EaeI y'GGCC_r Cuts at: 284 521 2621 2795 3186 3213 4558 284 Size: 237 2100 174 391 27 1345 762 Fragments arranged by size: 2100 1345 762 391 237 174 27 EagI C'GGCC_G Cuts at: 521 2621 521 Size: 2100 2936 EarI CTCTTCn'nnn_ Cuts at: 3059 3269 3059 Size: 210 4826 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 353 3244 3554 3700 4846 353 Size: 2891 310 146 1146 543 Fragments arranged by size: 2891 1146 543 310 146 EcoICRI GAG'CTC Cuts at: 1652 2048 1652 Size: 396 4640 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 439 1048 1145 1359 1585 1711 2042 2861 3293 Size: 609 97 214 226 126 331 819 432 Cuts at: 3293 4040 4308 439 Size: 747 268 1167 Fragments arranged by size: 1167 819 747 609 432 331 268 226 214 126 97 EcoNI CCTnn'n_nnAGG Cuts at: 967 967 Size: 5036 EcoO109I rG'GnC_Cy Cuts at: 644 950 960 1582 1869 1870 1881 1888 2017 Size: 306 10 622 287 1 11 7 129 Cuts at: 2017 2175 644 Size: 158 3505 Fragments arranged by size: 3505 622 306 287 158 129 11 10 7 1 EcoRI G'AATT_C Cuts at: 2040 2040 Size: 5036 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1842 3055 1842 Size: 1213 3823 FspI TGC'GCA Cuts at: 2817 2817 Size: 5036 HaeII r_GCGC'y Cuts at: 225 323 2470 2718 3740 4719 225 Size: 98 2147 248 1022 979 542 Fragments arranged by size: 2147 1022 979 542 248 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1285 1317 2878 2908 2910 2940 1285 Size: 32 1561 30 2 30 3381 Fragments arranged by size: 3381 1561 32 30 30 2 HincII GTy'rAC Cuts at: 2001 2075 2001 Size: 74 4962 KasI G'GCGC_C Cuts at: 2466 2714 2466 Size: 248 4788 KpnI G_GTAC'C Cuts at: 604 2056 604 Size: 1452 3584 MluI A'CGCG_T Cuts at: 4469 4469 Size: 5036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 608 3707 3891 608 Size: 3099 184 1753 Fragments arranged by size: 3099 1753 184 MscI TGG'CCA Cuts at: 2797 2797 Size: 5036 MslI CAynn'nnrTG Cuts at: 181 487 3025 3152 181 Size: 306 2538 127 2065 Fragments arranged by size: 2538 2065 306 127 MspA1I CmG'CkG Cuts at: 1068 2147 2461 2821 3834 4079 1068 Size: 1079 314 360 1013 245 2025 Fragments arranged by size: 2025 1079 1013 360 314 245 NaeI GCC'GGC Cuts at: 717 3217 717 Size: 2500 2536 NarI GG'CG_CC Cuts at: 2467 2715 2467 Size: 248 4788 NcoI C'CATG_G Cuts at: 107 815 2012 107 Size: 708 1197 3131 Fragments arranged by size: 3131 1197 708 NgoMIV G'CCGG_C Cuts at: 715 3215 715 Size: 2500 2536 NspI r_CATG'y Cuts at: 726 1302 1855 1929 2089 2589 3496 726 Size: 576 553 74 160 500 907 2266 Fragments arranged by size: 2266 907 576 553 500 160 74 PasI CC'CwG_GG Cuts at: 640 955 1875 640 Size: 315 920 3801 Fragments arranged by size: 3801 920 315 PciI A'CATG_T Cuts at: 1925 3492 1925 Size: 1567 3469 PmeI GTTT'AAAC Cuts at: 2035 2035 Size: 5036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 298 330 4175 4207 298 Size: 32 3845 32 1127 Fragments arranged by size: 3845 1127 32 32 PpuMI rG'GwC_Cy Cuts at: 644 950 1881 644 Size: 306 931 3799 Fragments arranged by size: 3799 931 306 PshAI GACnn'nnGTC Cuts at: 353 353 Size: 5036 PspOMI G'GGCC_C Cuts at: 1582 1869 1887 1582 Size: 287 18 4731 Fragments arranged by size: 4731 287 18 PspXI vC'TCGA_Gb Cuts at: 1936 1936 Size: 5036 PstI C_TGCA'G Cuts at: 295 1600 2083 2768 295 Size: 1305 483 685 2563 Fragments arranged by size: 2563 1305 685 483 PvuI CG_AT'CG Cuts at: 104 104 Size: 5036 PvuII CAG'CTG Cuts at: 1068 2461 2821 1068 Size: 1393 360 3283 Fragments arranged by size: 3283 1393 360 RsrII CG'GwC_CG Cuts at: 3231 3231 Size: 5036 SacI G_AGCT'C Cuts at: 1654 2050 1654 Size: 396 4640 SalI G'TCGA_C Cuts at: 2073 2073 Size: 5036 SanDI GG'GwC_CC Cuts at: 950 950 Size: 5036 SapI GCTCTTCn'nnn_ Cuts at: 3059 3269 3059 Size: 210 4826 SbfI CC_TGCA'GG Cuts at: 2083 2083 Size: 5036 ScaI AGT'ACT Cuts at: 301 1534 4553 301 Size: 1233 3019 784 Fragments arranged by size: 3019 1233 784 SexAI A'CCwGG_T Cuts at: 158 158 Size: 5036 SfcI C'TryA_G Cuts at: 33 291 1596 2079 2764 3757 3948 33 Size: 258 1305 483 685 993 191 1121 Fragments arranged by size: 1305 1121 993 685 483 258 191 SfoI GGC'GCC Cuts at: 2468 2716 2468 Size: 248 4788 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5036 SmaI CCC'GGG Cuts at: 2058 2058 Size: 5036 SmlI C'TyrA_G Cuts at: 1936 2191 3106 3598 3860 1936 Size: 255 915 492 262 3112 Fragments arranged by size: 3112 915 492 262 255 SpeI A'CTAG_T Cuts at: 67 67 Size: 5036 SphI G_CATG'C Cuts at: 726 2089 2589 726 Size: 1363 500 3173 Fragments arranged by size: 3173 1363 500 StuI AGG'CCT Cuts at: 1380 1380 Size: 5036 StyI C'CwwG_G Cuts at: 107 440 689 815 916 1896 2012 2170 2524 Size: 333 249 126 101 980 116 158 354 Cuts at: 2524 107 Size: 2619 Fragments arranged by size: 2619 980 354 333 249 158 126 116 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1090 2619 2752 3211 3420 1090 Size: 1529 133 459 209 2706 Fragments arranged by size: 2706 1529 459 209 133 TatI w'GTAC_w Cuts at: 299 1532 4551 299 Size: 1233 3019 784 Fragments arranged by size: 3019 1233 784 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1216 1248 4553 4585 1216 Size: 32 3305 32 1667 Fragments arranged by size: 3305 1667 32 32 Tth111I GACn'n_nGTC Cuts at: 2833 2833 Size: 5036 XbaI T'CTAG_A Cuts at: 2067 2067 Size: 5036 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1065 1167 1755 2531 1065 Size: 102 588 776 3570 Fragments arranged by size: 3570 776 588 102 XhoI C'TCGA_G Cuts at: 1936 1936 Size: 5036 XmaI C'CCGG_G Cuts at: 2056 2056 Size: 5036 XmnI GAAnn'nnTTC Cuts at: 1369 1369 Size: 5036 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AleI AloI AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI ZraI