Restriction map for pF1KB0038
 (Linear) (Six-base) MAPSORT of: KIBB0038.seq  Check: 9302  from: 1  to: 1167



KIBB0038.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     430    1167 
   Size:        430     737
 
Acc65I G'GTAC_C

Cuts at:      0     541    1167 
   Size:        541     626
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70    1167 
   Size:         70    1097
 
AleI CACnn'nnGTG

Cuts at:      0    1093    1167 
   Size:       1093      74
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     881    1167 
   Size:        881     286
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      13      45    1167 
   Size:         13      32    1122

  Fragments arranged by size: 

                1122      32      13
 
AlwNI CAG_nnn'CTG

Cuts at:      0     339     422    1167 
   Size:        339      83     745

  Fragments arranged by size: 

                 745     339      83
 
ApaI G_GGCC'C

Cuts at:      0     360     978    1167 
   Size:        360     618     189

  Fragments arranged by size: 

                 618     360     189
 
AvaI C'yCGr_G

Cuts at:      0     353     359     440     914    1167 
   Size:        353       6      81     474     253

  Fragments arranged by size: 

                 474     353     253      81       6
 
BamHI G'GATC_C

Cuts at:      0     308    1007    1167 
   Size:        308     699     160

  Fragments arranged by size: 

                 699     308     160
 
BanI G'GyrC_C

Cuts at:      0     541     892     910    1167 
   Size:        541     351      18     257

  Fragments arranged by size: 

                 541     351     257      18
 
BanII G_rGCy'C

Cuts at:      0     197     360     620     978    1057    1167 
   Size:        197     163     260     358      79     110

  Fragments arranged by size: 

                 358     260     197     163     110      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     762    1167 
   Size:        762     405
 
BbvCI CC'TCA_GC

Cuts at:      0     806    1167 
   Size:        806     361
 
BlpI GC'TnA_GC

Cuts at:      0     801    1167 
   Size:        801     366
 
Bme1580I G_kGCm'C

Cuts at:      0     360     897     978    1167 
   Size:        360     537      81     189

  Fragments arranged by size: 

                 537     360     189      81
 
BmgBI CAC'GTC

Cuts at:      0    1095    1167 
   Size:       1095      72
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     691    1167 
   Size:        691     476
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     895    1071    1093    1167 
   Size:        895     176      22      74

  Fragments arranged by size: 

                 895     176      74      22
 
Bpu10I CC'TnA_GC

Cuts at:      0     806     930    1167 
   Size:        806     124     237

  Fragments arranged by size: 

                 806     237     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    1167 
   Size:        661     506
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     449    1167 
   Size:        449     718
 
BsaWI w'CCGG_w

Cuts at:      0     129     305    1167 
   Size:        129     176     862

  Fragments arranged by size: 

                 862     176     129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      43     328     358    1030    1060    1167 
   Size:         13      30     285      30     672      30     107

  Fragments arranged by size: 

                 672     285     107      30      30      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     229    1167 
   Size:        229     938
 
BseYI C'CCAG_C

Cuts at:      0     565     652     940    1167 
   Size:        565      87     288     227

  Fragments arranged by size: 

                 565     288     227      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796    1167 
   Size:        796     371
 
BsiEI CG_ry'CG

Cuts at:      0      38    1167 
   Size:         38    1129
 
BsiHKAI G_wGCw'C

Cuts at:      0     197     620    1167 
   Size:        197     423     547

  Fragments arranged by size: 

                 547     423     197
 
Bsp1286I G_dGCh'C

Cuts at:      0     197     360     620     897     978    1057    1167 
   Size:        197     163     260     277      81      79     110

  Fragments arranged by size: 

                 277     260     197     163     110      81      79
 
BspEI T'CCGG_A

Cuts at:      0     305    1167 
   Size:        305     862
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430    1167 
   Size:        430     737
 
BsrBI CCG'CTC

Cuts at:      0      24     326     477     513     537    1167 
   Size:         24     302     151      36      24     630

  Fragments arranged by size: 

                 630     302     151      36      24      24
 
BsrFI r'CCGG_y

Cuts at:      0     658     727    1167 
   Size:        658      69     440

  Fragments arranged by size: 

                 658     440      69
 
BssSI C'ACGA_G

Cuts at:      0      85    1167 
   Size:         85    1082
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     422    1167 
   Size:        422     745
 
BstBI TT'CG_AA

Cuts at:      0     282     372    1167 
   Size:        282      90     795

  Fragments arranged by size: 

                 795     282      90
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     552     948    1026    1167 
   Size:        552     396      78     141

  Fragments arranged by size: 

                 552     396     141      78
 
BstYI r'GATC_y

Cuts at:      0     308     900    1007    1167 
   Size:        308     592     107     160

  Fragments arranged by size: 

                 592     308     160     107
 
Bsu36I CC'TnA_GG

Cuts at:      0     642    1167 
   Size:        642     525
 
BtgI C'CryG_G

Cuts at:      0     812    1167 
   Size:        812     355
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     984    1167 
   Size:        984     183
 
BtsI GCAGTG_nn'

Cuts at:      0     784    1167 
   Size:        784     383
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     195     230    1167 
   Size:        195      35     937

  Fragments arranged by size: 

                 937     195      35
 
DraI TTT'AAA

Cuts at:      0    1155    1167 
   Size:       1155      12
 
DraIII CAC_nnn'GTG

Cuts at:      0     738    1167 
   Size:        738     429
 
EaeI y'GGCC_r

Cuts at:      0      35     321     729     842    1016    1167 
   Size:         35     286     408     113     174     151

  Fragments arranged by size: 

                 408     286     174     151     113      35
 
EagI C'GGCC_G

Cuts at:      0      35    1167 
   Size:         35    1132
 
EarI CTCTTCn'nnn_

Cuts at:      0    1037    1167 
   Size:       1037     130
 
EcoICRI GAG'CTC

Cuts at:      0     195     618    1167 
   Size:        195     423     549

  Fragments arranged by size: 

                 549     423     195
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     895    1071    1093    1167 
   Size:         70     825     176      22      74

  Fragments arranged by size: 

                 825     176      74      70      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     633    1075    1167 
   Size:        633     442      92

  Fragments arranged by size: 

                 633     442      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0     336     366     435     598     638     934     954     974 
   Size:        336      30      69     163      40     296      20      20

Cuts at:    974     989    1032    1167 
   Size:         15      43     135

  Fragments arranged by size: 

                 336     296     163     135      69      43      40      30
                  20      20      15
 
HaeII r_GCGC'y

Cuts at:      0     659    1167 
   Size:        659     508
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     328     360    1030    1062    1084    1116    1167 
   Size:        328      32     670      32      22      32      51

  Fragments arranged by size: 

                 670     328      51      32      32      32      22
 
KpnI G_GTAC'C

Cuts at:      0     545    1167 
   Size:        545     622
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     945    1167 
   Size:        945     222
 
MscI TGG'CCA

Cuts at:      0    1018    1167 
   Size:       1018     149
 
MslI CAynn'nnrTG

Cuts at:      0    1093    1167 
   Size:       1093      74
 
MspA1I CmG'CkG

Cuts at:      0      13     747     805    1167 
   Size:         13     734      58     362

  Fragments arranged by size: 

                 734     362      58      13
 
NaeI GCC'GGC

Cuts at:      0     660     729    1167 
   Size:        660      69     438

  Fragments arranged by size: 

                 660     438      69
 
NgoMIV G'CCGG_C

Cuts at:      0     658     727    1167 
   Size:        658      69     440

  Fragments arranged by size: 

                 658     440      69
 
PasI CC'CwG_GG

Cuts at:      0     978    1167 
   Size:        978     189
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     339    1167 
   Size:        339     828
 
PfoI T'CCnGG_A

Cuts at:      0     143     620     872    1048    1167 
   Size:        143     477     252     176     119

  Fragments arranged by size: 

                 477     252     176     143     119
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45    1167 
   Size:         13      32    1122

  Fragments arranged by size: 

                1122      32      13
 
PpuMI rG'GwC_Cy

Cuts at:      0     336    1032    1167 
   Size:        336     696     135

  Fragments arranged by size: 

                 696     336     135
 
PspOMI G'GGCC_C

Cuts at:      0     356     974    1167 
   Size:        356     618     193

  Fragments arranged by size: 

                 618     356     193
 
PvuII CAG'CTG

Cuts at:      0     747     805    1167 
   Size:        747      58     362

  Fragments arranged by size: 

                 747     362      58
 
SacI G_AGCT'C

Cuts at:      0     197     620    1167 
   Size:        197     423     547

  Fragments arranged by size: 

                 547     423     197
 
SanDI GG'GwC_CC

Cuts at:      0    1032    1167 
   Size:       1032     135
 
SexAI A'CCwGG_T

Cuts at:      0     770    1167 
   Size:        770     397
 
SmaI CCC'GGG

Cuts at:      0     355     361     916    1167 
   Size:        355       6     555     251

  Fragments arranged by size: 

                 555     355     251       6
 
SmlI C'TyrA_G

Cuts at:      0     676    1167 
   Size:        676     491
 
StyI C'CwwG_G

Cuts at:      0     254    1143    1167 
   Size:        254     889      24

  Fragments arranged by size: 

                 889     254      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     467    1167 
   Size:        467     700
 
TatI w'GTAC_w

Cuts at:      0     113    1167 
   Size:        113    1054
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     819    1011    1167 
   Size:        819     192     156

  Fragments arranged by size: 

                 819     192     156
 
XmaI C'CCGG_G

Cuts at:      0     353     359     914    1167 
   Size:        353       6     555     253

  Fragments arranged by size: 

                 555     353     253       6
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI     AlfI     AloI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BlpI Bme1580I
    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EaeI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI   NgoMIV     PasI    PflMI
     PfoI     PpiI    PpuMI   PspOMI    PvuII     SacI    SanDI    SexAI
     SmaI     SmlI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BmrI     BmtI
    BsaAI    BsaBI    BsaHI    BsiWI     BsmI    BsmBI    BspHI    BsrDI
    BsrGI   BssHII   BstEII  BstZ17I     ClaI     DrdI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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