################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AccI AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbrPI BclI BfrI BlnI BsaAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrGI BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KspI MamI MfeI MluI MunI NaeI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 667 GACGTC GACGT^C Acc65I 2971 GGTACC G^GTACC AccII 587 CGCG CG^CG AcsI 379 GAATTT [GA]^AATT[CT] AflIII 1130 ACGTGT A^C[GA][CT]GT AocI 1534 CCTGAGG CC^T[ACGT]AGG ApoI 379 GAATTT [GA]^AATT[CT] Asp700I 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2971 GGTACC G^GTACC BbeI 2927 GGCGCC GGCGC^C BcgI 515 GCAGGTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 123 GCTGAGC GC^T[ACGT]AGC Bpu1102I 123 GCTGAGC GC^T[ACGT]AGC BsaI 1165 GAGACC GGTCTC(1/5) BspHI 701 TCATGA T^CATGA BsrDI 2250 CATTGC GCAATG(2/0) BsrFI 2945 ACCGGC [GA]^CCGG[CT] BssHII 586 GCGCGC G^CGCGC BstBI 1676 TTCGAA TT^CGAA BstUI 587 CGCG CG^CG Bsu36I 1534 CCTGAGG CC^T[ACGT]AGG CelII 123 GCTGAGC GC^T[ACGT]AGC Cfr10I 2945 ACCGGC [GA]^CCGG[CT] Eco81I 1534 CCTGAGG CC^T[ACGT]AGG HindIII 1231 AAGCTT A^AGCTT KasI 2927 GGCGCC G^GCGCC KpnI 2971 GGTACC GGTAC^C MvnI 587 CGCG CG^CG NarI 2927 GGCGCC GG^CGCC NdeI 1328 CATATG CA^TATG NspV 1676 TTCGAA TT^CGAA PshAI 1603 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 701 TCATGA T^CATGA SacI 126 GAGCTC GAGCT^C SapI 2145 GCTCTTC GCTCTTC(1/4) ScaI 324 AGTACT AGT^ACT SfoI 2927 GGCGCC GGC^GCC SfuI 1676 TTCGAA TT^CGAA SmaI 30 CCCGGG CCC^GGG SspI 946 AATATT AAT^ATT XcmI 2172 CCAGCGAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 30 CCCGGG C^CCGGG XmaI 30 CCCGGG C^CCGGG XmnI 288 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 929 TCCGGA T^CCGGA 2609 TCCGGA Alw44I 991 GTGCAC G^TGCAC 1351 GTGCAC ApaI 629 GGGCCC GGGCC^C 1845 GGGCCC ApaLI 991 GTGCAC G^TGCAC 1351 GTGCAC AvaI 30 CCCGGG C^[CT]CG[GA]G 3062 CTCGGG BalI 1457 TGGCCA TGG^CCA 1940 TGGCCA BanI 2927 GGCGCC G^G[CT][GA]CC 2971 GGTACC BciVI 2391 GGATAC GTATCC(6/5) 680 GTATCC BglI 547 GCCCTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2662 GCCAAGACGGC BseAI 929 TCCGGA T^CCGGA 2609 TCCGGA BsoBI 30 CCCGGG C^[CT]CG[GA]G 3062 CTCGGG BspEI 929 TCCGGA T^CCGGA 2609 TCCGGA BstAPI 1353 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2481 GCATGAGCTGC DraIII 994 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 1345 CACCAGGTG DrdI 667 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2596 GACAACAGCGTC EcoT14I 787 CCAAGG C^C[AT][AT]GG 2936 CCAAGG MluNI 1457 TGGCCA TGG^CCA 1940 TGGCCA MroI 929 TCCGGA T^CCGGA 2609 TCCGGA MscI 1457 TGGCCA TGG^CCA 1940 TGGCCA MslI 739 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 984 CATCGTGGTG SexAI 725 ACCTGGT A^CC[AT]GGT 1346 ACCAGGT SphI 968 GCATGC GCATG^C 1490 GCATGC StyI 787 CCAAGG C^C[AT][AT]GG 2936 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII