################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaI BseRI BsiEI BsiWI BsoBI Bsp1407I BspDI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MluI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 3118 CGCG CG^CG AccIII 2702 TCCGGA T^CCGGA AflIII 478 ACATGT A^C[GA][CT]GT Alw44I 1732 GTGCAC G^TGCAC ApaLI 1732 GTGCAC G^TGCAC BanI 2881 GGCACC G^G[CT][GA]CC BbvI 1123 GCAGC GCAGC(8/12) BclI 2657 TGATCA T^GATCA BglII 1761 AGATCT A^GATCT BsaBI 52 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2702 TCCGGA [AT]^CCGG[AT] BseAI 2702 TCCGGA T^CCGGA BsgI 2162 GTGCAG GTGCAG(16/14) BsmBI 1432 GAGACG CGTCTC(1/5) BspEI 2702 TCCGGA T^CCGGA BspLU11I 478 ACATGT A^CATGT BspMI 1487 GCAGGT ACCTGC(4/8) BstEII 2328 GGTAACC G^GT[ACGT]ACC BstPI 2328 GGTAACC G^GT[ACGT]ACC BstUI 3118 CGCG CG^CG BstXI 1528 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 2807 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 820 GACCATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 729 CCATGG C^C[GA][CT]GG EcoNI 547 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2328 GGTAACC G^GT[ACGT]ACC EcoRV 1939 GATATC GAT^ATC FauI 4663 CCCGC CCCGC(4/6) FbaI 2657 TGATCA T^GATCA Fnu4HI 1123 GCAGC GC^[ACGT]GC HapII 2703 CCGG C^CGG HincII 4676 GTCAAC GT[CT]^[GA]AC HindII 4676 GTCAAC GT[CT]^[GA]AC HindIII 1358 AAGCTT A^AGCTT HpaII 2703 CCGG C^CGG ItaI 1123 GCAGC GC^[ACGT]GC MamI 52 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2702 TCCGGA T^CCGGA MspA1I 1467 CAGCTG C[AC]G^C[GT]G MspI 2703 CCGG C^CGG MvnI 3118 CGCG CG^CG NcoI 729 CCATGG C^CATGG PstI 2428 CTGCAG CTGCA^G PvuII 1467 CAGCTG CAG^CTG SacI 421 GAGCTC GAGCT^C SapI 2764 GCTCTTC GCTCTTC(1/4) SnaBI 2371 TACGTA TAC^GTA TseI 1123 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 539 GTCTAC GT^[AC][GT]AC 3376 GTCTAC AseI 1779 ATTAAT AT^TAAT 4522 ATTAAT AsnI 1779 ATTAAT AT^TAAT 4522 ATTAAT AspHI 421 GAGCTC G[AT]GC[AT]^C 1732 GTGCAC BalI 4169 TGGCCA TGG^CCA 4318 TGGCCA BamHI 2537 GGATCC G^GATCC 3720 GGATCC BanII 421 GAGCTC G[GA]GC[CT]^C 4036 GGGCTC BpmI 3631 CTCCAG CTGGAG(16/14) 4203 CTCCAG BsaAI 686 CACGTA [CT]AC^GT[GA] 2371 TACGTA BsiHKAI 421 GAGCTC G[AT]GC[AT]^C 1732 GTGCAC BspHI 1943 TCATGA T^CATGA 2660 TCATGA BstAPI 3084 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3466 GCAGAACATGC EaeI 4169 TGGCCA [CT]^GGCC[GA] 4318 TGGCCA EcoT22I 3242 ATGCAT ATGCA^T 4411 ATGCAT MfeI 4430 CAATTG C^AATTG 4608 CAATTG MluNI 4169 TGGCCA TGG^CCA 4318 TGGCCA MscI 4169 TGGCCA TGG^CCA 4318 TGGCCA MunI 4430 CAATTG C^AATTG 4608 CAATTG NdeI 2777 CATATG CA^TATG 4408 CATATG NsiI 3242 ATGCAT ATGCA^T 4411 ATGCAT NspI 478 ACATGT [GA]CATG^[CT] 3471 ACATGC PshBI 1779 ATTAAT AT^TAAT 4522 ATTAAT RcaI 1943 TCATGA T^CATGA 2660 TCATGA ScaI 889 AGTACT AGT^ACT 2432 AGTACT SmlI 3477 CTCAAG C^T[CT][GA]AG 4370 CTCAAG SspI 2725 AATATT AAT^ATT 3847 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbsI BciVI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI EarI EcoRI EcoRII EcoT14I FokI HaeIII HinfI HphI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI