(Circular) (Six-base) MAPSORT of: pF1KA2004.seq Check: 159 from: 1 to: 7880 pF1KA2004 7880 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 648 3485 4918 648 Size: 2837 1433 3610 Fragments arranged by size: 3610 2837 1433 Acc65I G'GTAC_C Cuts at: 4896 4896 Size: 7880 AclI AA'CG_TT Cuts at: 7720 7720 Size: 7880 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 761 1420 5705 6137 6490 7152 761 Size: 659 4285 432 353 662 1489 Fragments arranged by size: 4285 1489 662 659 432 353 AflIII A'CryG_T Cuts at: 586 5232 7032 7313 586 Size: 4646 1800 281 1153 Fragments arranged by size: 4646 1800 1153 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 969 3375 4556 969 Size: 2406 1181 4293 Fragments arranged by size: 4293 2406 1181 AlwNI CAG_nnn'CTG Cuts at: 5185 6623 5185 Size: 1438 6442 ApaLI G'TGCA_C Cuts at: 1840 6718 1840 Size: 4878 3002 ApoI r'AATT_y Cuts at: 209 610 694 748 789 1120 1243 1907 2119 Size: 401 84 54 41 331 123 664 212 Cuts at: 2119 2128 2377 2926 2971 3150 3184 3734 3889 Size: 9 249 549 45 179 34 550 155 Cuts at: 3889 4266 4491 4884 7233 7489 209 Size: 377 225 393 2349 256 600 Fragments arranged by size: 2349 664 600 550 549 401 393 377 331 256 249 225 212 179 155 123 84 54 45 41 34 9 AseI AT'TA_AT Cuts at: 20 1888 4631 20 Size: 1868 2743 3269 Fragments arranged by size: 3269 2743 1868 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7880 AvaI C'yCGr_G Cuts at: 4900 4900 Size: 7880 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 575 608 575 Size: 33 7847 BamHI G'GATC_C Cuts at: 2645 3828 4905 2645 Size: 1183 1077 5620 Fragments arranged by size: 5620 1183 1077 BanI G'GyrC_C Cuts at: 2989 4896 5310 5558 5593 2989 Size: 1907 414 248 35 5276 Fragments arranged by size: 5276 1907 414 248 35 BanII G_rGCy'C Cuts at: 533 4148 4894 5924 533 Size: 3615 746 1030 2489 Fragments arranged by size: 3615 2489 1030 746 BbeI G_GCGC'C Cuts at: 5314 5562 5314 Size: 248 7632 BbsI GAAGACnn'nnnn_ Cuts at: 932 1706 2156 2984 4624 932 Size: 774 450 828 1640 4188 Fragments arranged by size: 4188 1640 828 774 450 BciVI GTATCCnnnnn_n' Cuts at: 3340 3662 4309 5778 6834 7880 3340 Size: 322 647 1469 1056 1046 3340 Fragments arranged by size: 3340 1469 1056 1046 647 322 BclI T'GATC_A Cuts at: 2765 5400 2765 Size: 2635 5245 BfrBI ATG'CAT Cuts at: 3352 4521 3352 Size: 1169 6711 BglII A'GATC_T Cuts at: 1869 5395 7432 1869 Size: 3526 2037 2317 Fragments arranged by size: 3526 2317 2037 BlpI GC'TnA_GC Cuts at: 4992 4992 Size: 7880 Bme1580I G_kGCm'C Cuts at: 1844 2992 5505 5598 6722 1844 Size: 1148 2513 93 1124 3002 Fragments arranged by size: 3002 2513 1148 1124 93 BmgBI CAC'GTC Cuts at: 228 228 Size: 7880 BmrI ACTGGGnnnn_n' Cuts at: 374 3429 3484 5092 5173 5508 7652 374 Size: 3055 55 1608 81 335 2144 602 Fragments arranged by size: 3055 2144 1608 602 335 81 55 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1789 6247 1789 Size: 4458 3422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 3724 4296 3724 Size: 572 7308 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3570 4463 5056 5935 6649 6947 3570 Size: 893 593 879 714 298 4503 Fragments arranged by size: 4503 893 879 714 593 298 BsaI GGTCTCn'nnnn_ Cuts at: 7584 7584 Size: 7880 BsaAI yAC'GTr Cuts at: 796 2481 5233 796 Size: 1685 2752 3443 Fragments arranged by size: 3443 2752 1685 BsaBI GATnn'nnATC Cuts at: 6 164 5394 7431 6 Size: 158 5230 2037 455 Fragments arranged by size: 5230 2037 455 158 BsaHI Gr'CG_yC Cuts at: 5311 5559 6261 7757 5311 Size: 248 702 1496 5434 Fragments arranged by size: 5434 1496 702 248 BsaWI w'CCGG_w Cuts at: 2810 5069 5590 6268 6679 6826 7100 2810 Size: 2259 521 678 411 147 274 3590 Fragments arranged by size: 3590 2259 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2420 2450 2420 Size: 30 7850 BseYI C'CCAG_C Cuts at: 1573 5305 6063 6728 7136 1573 Size: 3732 758 665 408 2317 Fragments arranged by size: 3732 2317 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 2291 Size: 7880 BsiEI CG_ry'CG Cuts at: 104 5422 5468 6698 104 Size: 5318 46 1230 1286 Fragments arranged by size: 5318 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 533 1844 4894 5672 5862 6722 533 Size: 1311 3050 778 190 860 1691 Fragments arranged by size: 3050 1691 1311 860 778 190 BsmI GAATG_Cn' Cuts at: 956 1057 3352 3592 956 Size: 101 2295 240 5244 Fragments arranged by size: 5244 2295 240 101 BsmBI CGTCTCn'nnnn_ Cuts at: 1534 1534 Size: 7880 Bsp1286I G_dGCh'C Cuts at: 533 1844 2992 4148 4894 5505 5598 5672 5862 Size: 1311 1148 1156 746 611 93 74 190 Cuts at: 5862 5924 6722 533 Size: 62 798 1691 Fragments arranged by size: 1691 1311 1156 1148 798 746 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 2810 2810 Size: 7880 BspHI T'CATG_A Cuts at: 2051 2768 6312 2051 Size: 717 3544 3619 Fragments arranged by size: 3619 3544 717 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1586 4930 5144 5446 5827 1586 Size: 3344 214 302 381 3639 Fragments arranged by size: 3639 3344 381 302 214 BsrBI CCG'CTC Cuts at: 50 6172 6226 7710 7875 50 Size: 6122 54 1484 165 55 Fragments arranged by size: 6122 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 494 762 1596 2261 2468 3414 5792 494 Size: 268 834 665 207 946 2378 2582 Fragments arranged by size: 2582 2378 946 834 665 268 207 BsrFI r'CCGG_y Cuts at: 5878 6059 5878 Size: 181 7699 BssSI C'ACGA_G Cuts at: 6151 6859 6151 Size: 708 7172 BstAPI GCAn_nnn'nTGC Cuts at: 3198 3580 5257 3198 Size: 382 1677 5821 Fragments arranged by size: 5821 1677 382 BstBI TT'CG_AA Cuts at: 6241 6241 Size: 7880 BstEII G'GTnAC_C Cuts at: 2436 2436 Size: 7880 BstXI CCAn_nnnn'nTGG Cuts at: 1643 1643 Size: 7880 BstYI r'GATC_y Cuts at: 1 1767 1803 1869 2645 3828 4905 5372 5395 Size: 1766 36 66 776 1183 1077 467 23 Cuts at: 5395 5730 5976 6282 6294 6380 7432 1 Size: 335 246 306 12 86 1052 449 Fragments arranged by size: 1766 1183 1077 1052 776 467 449 335 306 246 86 66 36 23 12 BtgI C'CryG_G Cuts at: 107 837 107 Size: 730 7150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3242 5830 6011 6174 3242 Size: 2588 181 163 4948 Fragments arranged by size: 4948 2588 181 163 BtsI GCAGTG_nn' Cuts at: 5123 5123 Size: 7880 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 956 991 956 Size: 35 7845 DraI TTT'AAA Cuts at: 1194 1206 4124 4879 1194 Size: 12 2918 755 4195 Fragments arranged by size: 4195 2918 755 12 DraIII CAC_nnn'GTG Cuts at: 2920 2920 Size: 7880 DrdI GACnn_nn'nnGTC Cuts at: 934 5586 6930 934 Size: 4652 1344 1884 Fragments arranged by size: 4652 1884 1344 EaeI y'GGCC_r Cuts at: 4277 4426 5465 5639 6030 6057 7402 4277 Size: 149 1039 174 391 27 1345 4755 Fragments arranged by size: 4755 1345 1039 391 174 149 27 EagI C'GGCC_G Cuts at: 5465 5465 Size: 7880 EarI CTCTTCn'nnn_ Cuts at: 725 1937 2202 2879 3965 5903 6113 725 Size: 1212 265 677 1086 1938 210 2492 Fragments arranged by size: 2492 1938 1212 1086 677 265 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 6088 6830 6976 7690 6088 Size: 742 146 714 6278 Fragments arranged by size: 6278 742 714 146 EcoICRI GAG'CTC Cuts at: 531 4892 531 Size: 4361 3519 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 761 1420 3724 4296 5705 6137 6490 7152 761 Size: 659 2304 572 1409 432 353 662 1489 Fragments arranged by size: 2304 1489 1409 662 659 572 432 353 EcoNI CCTnn'n_nnAGG Cuts at: 659 659 Size: 7880 EcoO109I rG'GnC_Cy Cuts at: 5019 5019 Size: 7880 EcoRI G'AATT_C Cuts at: 610 694 789 2128 4884 610 Size: 84 95 1339 2756 3606 Fragments arranged by size: 3606 2756 1339 95 84 EcoRV GAT'ATC Cuts at: 2049 2049 Size: 7880 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 283 1891 2228 3417 3736 4366 5899 283 Size: 1608 337 1189 319 630 1533 2264 Fragments arranged by size: 2264 1608 1533 1189 630 337 319 FspI TGC'GCA Cuts at: 5661 5661 Size: 7880 HaeII r_GCGC'y Cuts at: 5314 5562 6792 7563 5314 Size: 248 1230 771 5631 Fragments arranged by size: 5631 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1293 1325 1426 1458 1536 1568 3038 3070 3776 Size: 32 101 32 78 32 1470 32 706 Cuts at: 3776 3808 4801 4833 5722 5752 5754 5784 1293 Size: 32 993 32 889 30 2 30 3389 Fragments arranged by size: 3389 1470 993 889 706 101 78 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 4786 4919 4786 Size: 133 7747 HindIII A'AGCT_T Cuts at: 1466 1466 Size: 7880 KasI G'GCGC_C Cuts at: 5310 5558 5310 Size: 248 7632 KpnI G_GTAC'C Cuts at: 4900 4900 Size: 7880 MfeI C'AATT_G Cuts at: 4538 4716 4538 Size: 178 7702 MluI A'CGCG_T Cuts at: 7313 7313 Size: 7880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 617 963 3113 4326 6639 6823 617 Size: 346 2150 1213 2313 184 1674 Fragments arranged by size: 2313 2150 1674 1213 346 184 MscI TGG'CCA Cuts at: 4279 4428 5641 4279 Size: 149 1213 6518 Fragments arranged by size: 6518 1213 149 MslI CAynn'nnrTG Cuts at: 1901 3227 3239 3287 5869 5996 1901 Size: 1326 12 48 2582 127 3785 Fragments arranged by size: 3785 2582 1326 127 48 12 MspA1I CmG'CkG Cuts at: 1577 4991 5305 5665 6449 6694 1577 Size: 3414 314 360 784 245 2763 Fragments arranged by size: 3414 2763 784 360 314 245 NaeI GCC'GGC Cuts at: 6061 6061 Size: 7880 NarI GG'CG_CC Cuts at: 5311 5559 5311 Size: 248 7632 NcoI C'CATG_G Cuts at: 107 837 107 Size: 730 7150 NdeI CA'TA_TG Cuts at: 2886 4517 2886 Size: 1631 6249 NgoMIV G'CCGG_C Cuts at: 6059 6059 Size: 7880 NsiI A_TGCA'T Cuts at: 3354 4523 3354 Size: 1169 6711 NspI r_CATG'y Cuts at: 590 3583 4933 5433 7036 590 Size: 2993 1350 500 1603 1434 Fragments arranged by size: 2993 1603 1434 1350 500 PciI A'CATG_T Cuts at: 586 7032 586 Size: 6446 1434 PflMI CCAn_nnn'nTGG Cuts at: 2011 2329 3369 2011 Size: 318 1040 6522 Fragments arranged by size: 6522 1040 318 PmeI GTTT'AAAC Cuts at: 4879 4879 Size: 7880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3139 3171 6326 6358 3139 Size: 32 3155 32 4661 Fragments arranged by size: 4661 3155 32 32 PstI C_TGCA'G Cuts at: 2540 4927 5612 2540 Size: 2387 685 4808 Fragments arranged by size: 4808 2387 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 7880 PvuII CAG'CTG Cuts at: 1577 5305 5665 1577 Size: 3728 360 3792 Fragments arranged by size: 3792 3728 360 RsrII CG'GwC_CG Cuts at: 6075 6075 Size: 7880 SacI G_AGCT'C Cuts at: 533 4894 533 Size: 4361 3519 SalI G'TCGA_C Cuts at: 4917 4917 Size: 7880 SapI GCTCTTCn'nnn_ Cuts at: 2879 5903 6113 2879 Size: 3024 210 4646 Fragments arranged by size: 4646 3024 210 SbfI CC_TGCA'GG Cuts at: 4927 4927 Size: 7880 ScaI AGT'ACT Cuts at: 999 2542 7397 999 Size: 1543 4855 1482 Fragments arranged by size: 4855 1543 1482 SfcI C'TryA_G Cuts at: 33 649 2536 2558 4923 5608 6576 6767 33 Size: 616 1887 22 2365 685 968 191 1146 Fragments arranged by size: 2365 1887 1146 968 685 616 191 22 SfoI GGC'GCC Cuts at: 5312 5560 5312 Size: 248 7632 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7880 SmaI CCC'GGG Cuts at: 4902 4902 Size: 7880 SmlI C'TyrA_G Cuts at: 3585 4478 5035 5950 6664 6926 3585 Size: 893 557 915 714 262 4539 Fragments arranged by size: 4539 915 893 714 557 262 SnaBI TAC'GTA Cuts at: 2481 2481 Size: 7880 SpeI A'CTAG_T Cuts at: 67 67 Size: 7880 SphI G_CATG'C Cuts at: 4933 5433 4933 Size: 500 7380 SspI AAT'ATT Cuts at: 2835 3957 2835 Size: 1122 6758 StyI C'CwwG_G Cuts at: 107 837 875 2342 2843 5014 5368 107 Size: 730 38 1467 501 2171 354 2619 Fragments arranged by size: 2619 2171 1467 730 501 354 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1385 1859 5463 5596 6055 6264 1385 Size: 474 3604 133 459 209 3001 Fragments arranged by size: 3604 3001 474 459 209 133 TatI w'GTAC_w Cuts at: 493 997 1343 2487 2540 2570 7395 493 Size: 504 346 1144 53 30 4825 978 Fragments arranged by size: 4825 1144 978 504 346 53 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3027 3059 7397 7429 3027 Size: 32 4338 32 3478 Fragments arranged by size: 4338 3478 32 32 Tth111I GACn'n_nGTC Cuts at: 5677 5677 Size: 7880 XbaI T'CTAG_A Cuts at: 4911 4911 Size: 7880 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5375 5375 Size: 7880 XmaI C'CCGG_G Cuts at: 4900 4900 Size: 7880 XmnI GAAnn'nnTTC Cuts at: 2377 2836 2953 3154 2377 Size: 459 117 201 7103 Fragments arranged by size: 7103 459 201 117 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AleI AloI ApaI AscI AvrII BbvCI BcgI BglI BmtI Bpu10I BseRI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI FseI FspAI HpaI NheI NotI NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI