(Circular) (Six-base) MAPSORT of: pF1KA1454.seq Check: 7712 from: 1 to: 7310 pF1KA1454 7310 bp With 182 enzymes: SgfI * June 20, 2008 10:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3103 3103 Size: 7310 AccI GT'mk_AC Cuts at: 331 4348 331 Size: 4017 3293 Acc65I G'GTAC_C Cuts at: 4326 4326 Size: 7310 AclI AA'CG_TT Cuts at: 7150 7150 Size: 7310 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 547 797 911 1322 1460 1493 1795 2495 2730 Size: 250 114 411 138 33 302 700 235 Cuts at: 2730 2803 3613 5135 5567 5920 6582 547 Size: 73 810 1522 432 353 662 1275 Fragments arranged by size: 1522 1275 810 700 662 432 411 353 302 250 235 138 114 73 33 AfeI AGC'GCT Cuts at: 124 124 Size: 7310 AflIII A'CryG_T Cuts at: 4662 6462 6743 4662 Size: 1800 281 5229 Fragments arranged by size: 5229 1800 281 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 7310 AleI CACnn'nnGTG Cuts at: 1109 3028 1109 Size: 1919 5391 AlwNI CAG_nnn'CTG Cuts at: 4615 6053 4615 Size: 1438 5872 ApaLI G'TGCA_C Cuts at: 6148 6148 Size: 7310 ApoI r'AATT_y Cuts at: 708 1170 1294 1350 1676 2339 2784 3047 4314 Size: 462 124 56 326 663 445 263 1267 Cuts at: 4314 6663 6919 708 Size: 2349 256 1099 Fragments arranged by size: 2349 1267 1099 663 462 445 326 263 256 124 56 AseI AT'TA_AT Cuts at: 20 20 Size: 7310 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7310 AvaI C'yCGr_G Cuts at: 2255 4330 2255 Size: 2075 5235 AvrII C'CTAG_G Cuts at: 2910 2910 Size: 7310 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3160 3193 3160 Size: 33 7277 BamHI G'GATC_C Cuts at: 3603 4335 3603 Size: 732 6578 BanI G'GyrC_C Cuts at: 377 1613 3685 4326 4740 4988 5023 377 Size: 1236 2072 641 414 248 35 2664 Fragments arranged by size: 2664 2072 1236 641 414 248 35 BanII G_rGCy'C Cuts at: 405 2142 4324 5354 405 Size: 1737 2182 1030 2361 Fragments arranged by size: 2361 2182 1737 1030 BbeI G_GCGC'C Cuts at: 1617 4744 4992 1617 Size: 3127 248 3935 Fragments arranged by size: 3935 3127 248 BbsI GAAGACnn'nnnn_ Cuts at: 586 2094 586 Size: 1508 5802 BbvCI CC'TCA_GC Cuts at: 4253 4253 Size: 7310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1567 1601 1567 Size: 34 7276 BciVI GTATCCnnnnn_n' Cuts at: 5208 6264 7310 5208 Size: 1056 1046 5208 Fragments arranged by size: 5208 1056 1046 BclI T'GATC_A Cuts at: 4043 4830 4043 Size: 787 6523 BfrBI ATG'CAT Cuts at: 1686 1686 Size: 7310 BglII A'GATC_T Cuts at: 2835 4825 6862 2835 Size: 1990 2037 3283 Fragments arranged by size: 3283 2037 1990 BlpI GC'TnA_GC Cuts at: 2411 2416 4422 2411 Size: 5 2006 5299 Fragments arranged by size: 5299 2006 5 Bme1580I G_kGCm'C Cuts at: 4935 5028 6152 4935 Size: 93 1124 6093 Fragments arranged by size: 6093 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 420 825 1131 3472 4522 4603 4938 7082 420 Size: 405 306 2341 1050 81 335 2144 648 Fragments arranged by size: 2341 2144 1050 648 405 335 306 81 BmtI G_CTAG'C Cuts at: 1390 1390 Size: 7310 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5677 5677 Size: 7310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 359 1649 2651 3668 4129 4151 359 Size: 1290 1002 1017 461 22 3518 Fragments arranged by size: 3518 1290 1017 1002 461 22 Bpu10I CC'TnA_GC Cuts at: 1774 1967 2753 4253 1774 Size: 193 786 1500 4831 Fragments arranged by size: 4831 1500 786 193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2332 4252 4486 5365 6079 6377 2332 Size: 1920 234 879 714 298 3265 Fragments arranged by size: 3265 1920 879 714 298 234 BsaI GGTCTCn'nnnn_ Cuts at: 895 7014 895 Size: 6119 1191 BsaAI yAC'GTr Cuts at: 2110 3420 4663 2110 Size: 1310 1243 4757 Fragments arranged by size: 4757 1310 1243 BsaBI GATnn'nnATC Cuts at: 6 3488 4824 6861 6 Size: 3482 1336 2037 455 Fragments arranged by size: 3482 2037 1336 455 BsaHI Gr'CG_yC Cuts at: 642 1614 4741 4989 5691 7187 642 Size: 972 3127 248 702 1496 765 Fragments arranged by size: 3127 1496 972 765 702 248 BsaWI w'CCGG_w Cuts at: 1841 4499 5020 5698 6109 6256 6530 1841 Size: 2658 521 678 411 147 274 2621 Fragments arranged by size: 2658 2621 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 178 731 178 Size: 553 6757 BseYI C'CCAG_C Cuts at: 3018 3700 3867 4735 5493 6158 6566 3018 Size: 682 167 868 758 665 408 3762 Fragments arranged by size: 3762 868 758 682 665 408 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 874 1945 2143 3988 874 Size: 1071 198 1845 4196 Fragments arranged by size: 4196 1845 1071 198 BsiEI CG_ry'CG Cuts at: 104 3978 4852 4898 6128 104 Size: 3874 874 46 1230 1286 Fragments arranged by size: 3874 1286 1230 874 46 BsiHKAI G_wGCw'C Cuts at: 405 2142 4324 5102 5292 6152 405 Size: 1737 2182 778 190 860 1563 Fragments arranged by size: 2182 1737 1563 860 778 190 BsmI GAATG_Cn' Cuts at: 3841 3841 Size: 7310 Bsp1286I G_dGCh'C Cuts at: 405 2142 4324 4935 5028 5102 5292 5354 6152 Size: 1737 2182 611 93 74 190 62 798 Cuts at: 6152 405 Size: 1563 Fragments arranged by size: 2182 1737 1563 798 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 1841 1841 Size: 7310 BspHI T'CATG_A Cuts at: 135 2019 3906 5742 135 Size: 1884 1887 1836 1703 Fragments arranged by size: 1887 1884 1836 1703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1662 3103 4360 4574 4876 5257 1662 Size: 1441 1257 214 302 381 3715 Fragments arranged by size: 3715 1441 1257 381 302 214 BsrBI CCG'CTC Cuts at: 50 2154 2697 5602 5656 7140 7305 50 Size: 2104 543 2905 54 1484 165 55 Fragments arranged by size: 2905 2104 1484 543 165 55 54 BsrDI GCAATG_nn' Cuts at: 1421 2502 3075 3545 5222 1421 Size: 1081 573 470 1677 3509 Fragments arranged by size: 3509 1677 1081 573 470 BsrFI r'CCGG_y Cuts at: 5308 5489 5308 Size: 181 7129 BsrGI T'GTAC_A Cuts at: 3544 3544 Size: 7310 BssSI C'ACGA_G Cuts at: 1601 5581 6289 1601 Size: 3980 708 2622 Fragments arranged by size: 3980 2622 708 BstAPI GCAn_nnn'nTGC Cuts at: 4687 4687 Size: 7310 BstBI TT'CG_AA Cuts at: 1200 2367 5671 1200 Size: 1167 3304 2839 Fragments arranged by size: 3304 2839 1167 BstXI CCAn_nnnn'nTGG Cuts at: 3747 3747 Size: 7310 BstYI r'GATC_y Cuts at: 1 2835 3603 4335 4802 4825 5160 5406 5712 Size: 2834 768 732 467 23 335 246 306 Cuts at: 5712 5724 5810 6862 1 Size: 12 86 1052 449 Fragments arranged by size: 2834 1052 768 732 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 1355 1565 3681 4036 107 Size: 1248 210 2116 355 3381 Fragments arranged by size: 3381 2116 1248 355 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5260 5441 5604 5260 Size: 181 163 6966 Fragments arranged by size: 6966 181 163 BtsI GCAGTG_nn' Cuts at: 314 950 1542 2427 2488 3126 3704 3873 4553 Size: 636 592 885 61 638 578 169 680 Cuts at: 4553 314 Size: 3071 Fragments arranged by size: 3071 885 680 638 636 592 578 169 61 DraI TTT'AAA Cuts at: 4309 4309 Size: 7310 DraIII CAC_nnn'GTG Cuts at: 3379 3379 Size: 7310 DrdI GACnn_nn'nnGTC Cuts at: 5016 6360 5016 Size: 1344 5966 EaeI y'GGCC_r Cuts at: 3500 3749 3854 3975 4136 4895 5069 5460 5487 Size: 249 105 121 161 759 174 391 27 Cuts at: 5487 6832 3500 Size: 1345 3978 Fragments arranged by size: 3978 1345 759 391 249 174 161 121 105 27 EagI C'GGCC_G Cuts at: 3975 4895 3975 Size: 920 6390 EarI CTCTTCn'nnn_ Cuts at: 1376 1787 2069 2179 2318 2514 2687 3632 3682 Size: 411 282 110 139 196 173 945 50 Cuts at: 3682 3794 5333 5543 1376 Size: 112 1539 210 3143 Fragments arranged by size: 3143 1539 945 411 282 210 196 173 139 112 110 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 5518 6260 6406 7120 5518 Size: 742 146 714 5708 Fragments arranged by size: 5708 742 714 146 EcoICRI GAG'CTC Cuts at: 403 2140 4322 403 Size: 1737 2182 3391 Fragments arranged by size: 3391 2182 1737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 359 547 797 911 1322 1460 1493 1649 1795 Size: 188 250 114 411 138 33 156 146 Cuts at: 1795 2495 2651 2730 2803 3613 3668 4129 4151 Size: 700 156 79 73 810 55 461 22 Cuts at: 4151 5135 5567 5920 6582 359 Size: 984 432 353 662 1087 Fragments arranged by size: 1087 984 810 700 662 461 432 411 353 250 188 156 156 146 138 114 79 73 55 33 22 EcoNI CCTnn'n_nnAGG Cuts at: 3907 3907 Size: 7310 EcoO109I rG'GnC_Cy Cuts at: 3405 4449 3405 Size: 1044 6266 EcoRI G'AATT_C Cuts at: 1170 1676 3047 4314 1170 Size: 506 1371 1267 4166 Fragments arranged by size: 4166 1371 1267 506 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2833 3165 4246 5329 2833 Size: 332 1081 1083 4814 Fragments arranged by size: 4814 1083 1081 332 FspI TGC'GCA Cuts at: 5091 5091 Size: 7310 HaeII r_GCGC'y Cuts at: 126 1617 4744 4992 6222 6993 126 Size: 1491 3127 248 1230 771 443 Fragments arranged by size: 3127 1491 1230 771 443 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 573 605 642 674 2014 2046 2854 2886 3288 Size: 32 37 32 1340 32 808 32 402 Cuts at: 3288 3320 3609 3641 3817 3849 4155 4187 5152 Size: 32 289 32 176 32 306 32 965 Cuts at: 5152 5182 5184 5214 573 Size: 30 2 30 2669 Fragments arranged by size: 2669 1340 965 808 402 306 289 176 37 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1454 3179 3942 4349 1454 Size: 1725 763 407 4415 Fragments arranged by size: 4415 1725 763 407 HindIII A'AGCT_T Cuts at: 702 1807 2675 2815 702 Size: 1105 868 140 5197 Fragments arranged by size: 5197 1105 868 140 HpaI GTT'AAC Cuts at: 1454 1454 Size: 7310 KasI G'GCGC_C Cuts at: 1613 4740 4988 1613 Size: 3127 248 3935 Fragments arranged by size: 3935 3127 248 KpnI G_GTAC'C Cuts at: 4330 4330 Size: 7310 MfeI C'AATT_G Cuts at: 1721 1937 1721 Size: 216 7094 MluI A'CGCG_T Cuts at: 6743 6743 Size: 7310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1035 6069 6253 1035 Size: 5034 184 2092 Fragments arranged by size: 5034 2092 184 MscI TGG'CCA Cuts at: 3502 3751 3856 4138 5071 3502 Size: 249 105 282 933 5741 Fragments arranged by size: 5741 933 282 249 105 MslI CAynn'nnrTG Cuts at: 612 1109 1685 3028 3745 5299 5426 612 Size: 497 576 1343 717 1554 127 2496 Fragments arranged by size: 2496 1554 1343 717 576 497 127 MspA1I CmG'CkG Cuts at: 2302 3622 4421 4735 5095 5879 6124 2302 Size: 1320 799 314 360 784 245 3488 Fragments arranged by size: 3488 1320 799 784 360 314 245 NaeI GCC'GGC Cuts at: 5491 5491 Size: 7310 NarI GG'CG_CC Cuts at: 1614 4741 4989 1614 Size: 3127 248 3935 Fragments arranged by size: 3935 3127 248 NcoI C'CATG_G Cuts at: 107 1355 1565 3681 107 Size: 1248 210 2116 3736 Fragments arranged by size: 3736 2116 1248 210 NdeI CA'TA_TG Cuts at: 3659 3659 Size: 7310 NgoMIV G'CCGG_C Cuts at: 5489 5489 Size: 7310 NheI G'CTAG_C Cuts at: 1386 1386 Size: 7310 NsiI A_TGCA'T Cuts at: 1688 1688 Size: 7310 NspI r_CATG'y Cuts at: 1686 2434 3520 3538 4363 4863 6466 1686 Size: 748 1086 18 825 500 1603 2530 Fragments arranged by size: 2530 1603 1086 825 748 500 18 PciI A'CATG_T Cuts at: 6462 6462 Size: 7310 PflMI CCAn_nnn'nTGG Cuts at: 1571 1672 1571 Size: 101 7209 PfoI T'CCnGG_A Cuts at: 636 636 Size: 7310 PmeI GTTT'AAAC Cuts at: 4309 4309 Size: 7310 PmlI CAC'GTG Cuts at: 2110 2110 Size: 7310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5756 5788 5756 Size: 32 7278 PpuMI rG'GwC_Cy Cuts at: 3405 3405 Size: 7310 PsiI TTA'TAA Cuts at: 1990 2807 1990 Size: 817 6493 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1056 1088 1056 Size: 32 7278 PstI C_TGCA'G Cuts at: 309 3121 3515 3880 3998 4357 5042 309 Size: 2812 394 365 118 359 685 2577 Fragments arranged by size: 2812 2577 685 394 365 359 118 PvuI CG_AT'CG Cuts at: 104 104 Size: 7310 PvuII CAG'CTG Cuts at: 2302 3622 4735 5095 2302 Size: 1320 1113 360 4517 Fragments arranged by size: 4517 1320 1113 360 RsrII CG'GwC_CG Cuts at: 5505 5505 Size: 7310 SacI G_AGCT'C Cuts at: 405 2142 4324 405 Size: 1737 2182 3391 Fragments arranged by size: 3391 2182 1737 SalI G'TCGA_C Cuts at: 4347 4347 Size: 7310 SapI GCTCTTCn'nnn_ Cuts at: 1376 2069 2687 3632 3794 5333 5543 1376 Size: 693 618 945 162 1539 210 3143 Fragments arranged by size: 3143 1539 945 693 618 210 162 SbfI CC_TGCA'GG Cuts at: 4357 4357 Size: 7310 ScaI AGT'ACT Cuts at: 303 4052 6827 303 Size: 3749 2775 786 Fragments arranged by size: 3749 2775 786 SexAI A'CCwGG_T Cuts at: 3956 3956 Size: 7310 SfcI C'TryA_G Cuts at: 33 305 1076 2068 3117 3511 3876 3994 4353 Size: 272 771 992 1049 394 365 118 359 Cuts at: 4353 5038 6006 6197 33 Size: 685 968 191 1146 Fragments arranged by size: 1146 1049 992 968 771 685 394 365 359 272 191 118 SfoI GGC'GCC Cuts at: 1615 4742 4990 1615 Size: 3127 248 3935 Fragments arranged by size: 3935 3127 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7310 SmaI CCC'GGG Cuts at: 2257 4332 2257 Size: 2075 5235 SmlI C'TyrA_G Cuts at: 2347 4231 4465 5380 6094 6356 2347 Size: 1884 234 915 714 262 3301 Fragments arranged by size: 3301 1884 915 714 262 234 SpeI A'CTAG_T Cuts at: 67 67 Size: 7310 SphI G_CATG'C Cuts at: 3520 4363 4863 3520 Size: 843 500 5967 Fragments arranged by size: 5967 843 500 StuI AGG'CCT Cuts at: 862 921 4261 862 Size: 59 3340 3911 Fragments arranged by size: 3911 3340 59 StyI C'CwwG_G Cuts at: 107 1355 1565 2910 3681 3716 4212 4444 4798 Size: 1248 210 1345 771 35 496 232 354 Cuts at: 4798 107 Size: 2619 Fragments arranged by size: 2619 1345 1248 771 496 354 232 210 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3962 4893 5026 5485 5694 3962 Size: 931 133 459 209 5578 Fragments arranged by size: 5578 931 459 209 133 TatI w'GTAC_w Cuts at: 301 1150 2633 2659 2850 3544 4050 6825 301 Size: 849 1483 26 191 694 506 2775 786 Fragments arranged by size: 2775 1483 849 786 694 506 191 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 632 664 3696 3728 6827 6859 632 Size: 32 3032 32 3099 32 1083 Fragments arranged by size: 3099 3032 1083 32 32 32 Tth111I GACn'n_nGTC Cuts at: 5107 5107 Size: 7310 XbaI T'CTAG_A Cuts at: 1398 2142 4341 1398 Size: 744 2199 4367 Fragments arranged by size: 4367 2199 744 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3864 4805 3864 Size: 941 6369 XmaI C'CCGG_G Cuts at: 2255 4330 2255 Size: 2075 5235 XmnI GAAnn'nnTTC Cuts at: 1295 2363 1295 Size: 1068 6242 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AflII AgeI AlfI AloI ApaI AscI BglI BmgBI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI NotI NruI PacI PasI PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI