(Linear) (Six-base) MAPSORT of: KIAA1454.seq Check: 8425 from: 1 to: 4197 KIAA1454.orf With 182 enzymes: SgfI * July 18, 2007 13:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2995 4197 Size: 2995 1202 AccI GT'mk_AC Cuts at: 0 223 4197 Size: 223 3974 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 689 803 1214 1352 1385 1687 2387 Size: 439 250 114 411 138 33 302 700 Cuts at: 2387 2622 2695 3505 4197 Size: 235 73 810 692 Fragments arranged by size: 810 700 692 439 411 302 250 235 138 114 73 33 AfeI AGC'GCT Cuts at: 0 16 4197 Size: 16 4181 AhdI GACnn_n'nnGTC Cuts at: 0 4061 4197 Size: 4061 136 AleI CACnn'nnGTG Cuts at: 0 1001 2920 4197 Size: 1001 1919 1277 Fragments arranged by size: 1919 1277 1001 ApoI r'AATT_y Cuts at: 0 600 1062 1186 1242 1568 2231 2676 2939 Size: 600 462 124 56 326 663 445 263 Cuts at: 2939 4197 Size: 1258 Fragments arranged by size: 1258 663 600 462 445 326 263 124 56 AvaI C'yCGr_G Cuts at: 0 2147 4197 Size: 2147 2050 AvrII C'CTAG_G Cuts at: 0 2802 4197 Size: 2802 1395 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3052 3085 4197 Size: 3052 33 1112 Fragments arranged by size: 3052 1112 33 BamHI G'GATC_C Cuts at: 0 3495 4197 Size: 3495 702 BanI G'GyrC_C Cuts at: 0 269 1505 3577 4197 Size: 269 1236 2072 620 Fragments arranged by size: 2072 1236 620 269 BanII G_rGCy'C Cuts at: 0 297 2034 4197 Size: 297 1737 2163 Fragments arranged by size: 2163 1737 297 BbeI G_GCGC'C Cuts at: 0 1509 4197 Size: 1509 2688 BbsI GAAGACnn'nnnn_ Cuts at: 0 478 1986 4197 Size: 478 1508 2211 Fragments arranged by size: 2211 1508 478 BbvCI CC'TCA_GC Cuts at: 0 4145 4197 Size: 4145 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1459 1493 4197 Size: 1459 34 2704 Fragments arranged by size: 2704 1459 34 BclI T'GATC_A Cuts at: 0 3935 4197 Size: 3935 262 BfrBI ATG'CAT Cuts at: 0 1578 4197 Size: 1578 2619 BglII A'GATC_T Cuts at: 0 2727 4197 Size: 2727 1470 BlpI GC'TnA_GC Cuts at: 0 2303 2308 4197 Size: 2303 5 1889 Fragments arranged by size: 2303 1889 5 BmrI ACTGGGnnnn_n' Cuts at: 0 312 717 1023 3364 4197 Size: 312 405 306 2341 833 Fragments arranged by size: 2341 833 405 312 306 BmtI G_CTAG'C Cuts at: 0 1282 4197 Size: 1282 2915 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 1541 2543 3560 4021 4043 4197 Size: 251 1290 1002 1017 461 22 154 Fragments arranged by size: 1290 1017 1002 461 251 154 22 Bpu10I CC'TnA_GC Cuts at: 0 1666 1859 2645 4145 4197 Size: 1666 193 786 1500 52 Fragments arranged by size: 1666 1500 786 193 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2224 4144 4197 Size: 2224 1920 53 Fragments arranged by size: 2224 1920 53 BsaI GGTCTCn'nnnn_ Cuts at: 0 787 4197 Size: 787 3410 BsaAI yAC'GTr Cuts at: 0 2002 3312 4197 Size: 2002 1310 885 Fragments arranged by size: 2002 1310 885 BsaBI GATnn'nnATC Cuts at: 0 3380 4197 Size: 3380 817 BsaHI Gr'CG_yC Cuts at: 0 534 1506 4197 Size: 534 972 2691 Fragments arranged by size: 2691 972 534 BsaWI w'CCGG_w Cuts at: 0 1733 4197 Size: 1733 2464 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 70 623 4197 Size: 70 553 3574 Fragments arranged by size: 3574 553 70 BseYI C'CCAG_C Cuts at: 0 2910 3592 3759 4197 Size: 2910 682 167 438 Fragments arranged by size: 2910 682 438 167 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 1837 2035 3880 4197 Size: 766 1071 198 1845 317 Fragments arranged by size: 1845 1071 766 317 198 BsiEI CG_ry'CG Cuts at: 0 3870 4197 Size: 3870 327 BsiHKAI G_wGCw'C Cuts at: 0 297 2034 4197 Size: 297 1737 2163 Fragments arranged by size: 2163 1737 297 BsmI GAATG_Cn' Cuts at: 0 3733 4197 Size: 3733 464 Bsp1286I G_dGCh'C Cuts at: 0 297 2034 4197 Size: 297 1737 2163 Fragments arranged by size: 2163 1737 297 BspEI T'CCGG_A Cuts at: 0 1733 4197 Size: 1733 2464 BspHI T'CATG_A Cuts at: 0 27 1911 3798 4197 Size: 27 1884 1887 399 Fragments arranged by size: 1887 1884 399 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1554 2995 4197 Size: 1554 1441 1202 Fragments arranged by size: 1554 1441 1202 BsrBI CCG'CTC Cuts at: 0 2046 2589 4197 Size: 2046 543 1608 Fragments arranged by size: 2046 1608 543 BsrDI GCAATG_nn' Cuts at: 0 1313 2394 2967 3437 4197 Size: 1313 1081 573 470 760 Fragments arranged by size: 1313 1081 760 573 470 BsrGI T'GTAC_A Cuts at: 0 3436 4197 Size: 3436 761 BssSI C'ACGA_G Cuts at: 0 1493 4197 Size: 1493 2704 BstBI TT'CG_AA Cuts at: 0 1092 2259 4197 Size: 1092 1167 1938 Fragments arranged by size: 1938 1167 1092 BstXI CCAn_nnnn'nTGG Cuts at: 0 3639 4197 Size: 3639 558 BstYI r'GATC_y Cuts at: 0 2727 3495 4197 Size: 2727 768 702 Fragments arranged by size: 2727 768 702 BtgI C'CryG_G Cuts at: 0 1247 1457 3573 3928 4197 Size: 1247 210 2116 355 269 Fragments arranged by size: 2116 1247 355 269 210 BtsI GCAGTG_nn' Cuts at: 0 206 842 1434 2319 2380 3018 3596 3765 Size: 206 636 592 885 61 638 578 169 Cuts at: 3765 4197 Size: 432 Fragments arranged by size: 885 638 636 592 578 432 206 169 61 DraIII CAC_nnn'GTG Cuts at: 0 3271 4197 Size: 3271 926 EaeI y'GGCC_r Cuts at: 0 3392 3641 3746 3867 4028 4197 Size: 3392 249 105 121 161 169 Fragments arranged by size: 3392 249 169 161 121 105 EagI C'GGCC_G Cuts at: 0 3867 4197 Size: 3867 330 EarI CTCTTCn'nnn_ Cuts at: 0 1268 1679 1961 2071 2210 2406 2579 3524 Size: 1268 411 282 110 139 196 173 945 Cuts at: 3524 3574 3686 4197 Size: 50 112 511 Fragments arranged by size: 1268 945 511 411 282 196 173 139 112 110 50 EcoICRI GAG'CTC Cuts at: 0 295 2032 4197 Size: 295 1737 2165 Fragments arranged by size: 2165 1737 295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 439 689 803 1214 1352 1385 1541 Size: 251 188 250 114 411 138 33 156 Cuts at: 1541 1687 2387 2543 2622 2695 3505 3560 4021 Size: 146 700 156 79 73 810 55 461 Cuts at: 4021 4043 4197 Size: 22 154 Fragments arranged by size: 810 700 461 411 251 250 188 156 156 154 146 138 114 79 73 55 33 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 3799 4197 Size: 3799 398 EcoO109I rG'GnC_Cy Cuts at: 0 3297 4197 Size: 3297 900 EcoRI G'AATT_C Cuts at: 0 1062 1568 2939 4197 Size: 1062 506 1371 1258 Fragments arranged by size: 1371 1258 1062 506 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2725 3057 4138 4197 Size: 2725 332 1081 59 Fragments arranged by size: 2725 1081 332 59 HaeII r_GCGC'y Cuts at: 0 18 1509 4197 Size: 18 1491 2688 Fragments arranged by size: 2688 1491 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 465 497 534 566 1906 1938 2746 2778 Size: 465 32 37 32 1340 32 808 32 Cuts at: 2778 3180 3212 3501 3533 3709 3741 4047 4079 Size: 402 32 289 32 176 32 306 32 Cuts at: 4079 4197 Size: 118 Fragments arranged by size: 1340 808 465 402 306 289 176 118 37 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1346 3071 3834 4197 Size: 1346 1725 763 363 Fragments arranged by size: 1725 1346 763 363 HindIII A'AGCT_T Cuts at: 0 594 1699 2567 2707 4197 Size: 594 1105 868 140 1490 Fragments arranged by size: 1490 1105 868 594 140 HpaI GTT'AAC Cuts at: 0 1346 4197 Size: 1346 2851 KasI G'GCGC_C Cuts at: 0 1505 4197 Size: 1505 2692 MfeI C'AATT_G Cuts at: 0 1613 1829 4197 Size: 1613 216 2368 Fragments arranged by size: 2368 1613 216 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 927 4197 Size: 927 3270 MscI TGG'CCA Cuts at: 0 3394 3643 3748 4030 4197 Size: 3394 249 105 282 167 Fragments arranged by size: 3394 282 249 167 105 MslI CAynn'nnrTG Cuts at: 0 504 1001 1577 2920 3637 4197 Size: 504 497 576 1343 717 560 Fragments arranged by size: 1343 717 576 560 504 497 MspA1I CmG'CkG Cuts at: 0 2194 3514 4197 Size: 2194 1320 683 Fragments arranged by size: 2194 1320 683 NarI GG'CG_CC Cuts at: 0 1506 4197 Size: 1506 2691 NcoI C'CATG_G Cuts at: 0 1247 1457 3573 4197 Size: 1247 210 2116 624 Fragments arranged by size: 2116 1247 624 210 NdeI CA'TA_TG Cuts at: 0 3551 4197 Size: 3551 646 NheI G'CTAG_C Cuts at: 0 1278 4197 Size: 1278 2919 NsiI A_TGCA'T Cuts at: 0 1580 4197 Size: 1580 2617 NspI r_CATG'y Cuts at: 0 1578 2326 3412 3430 4197 Size: 1578 748 1086 18 767 Fragments arranged by size: 1578 1086 767 748 18 PflMI CCAn_nnn'nTGG Cuts at: 0 1463 1564 4197 Size: 1463 101 2633 Fragments arranged by size: 2633 1463 101 PfoI T'CCnGG_A Cuts at: 0 528 4197 Size: 528 3669 PmlI CAC'GTG Cuts at: 0 2002 4197 Size: 2002 2195 PpuMI rG'GwC_Cy Cuts at: 0 3297 4197 Size: 3297 900 PsiI TTA'TAA Cuts at: 0 1882 2699 4197 Size: 1882 817 1498 Fragments arranged by size: 1882 1498 817 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 948 980 4197 Size: 948 32 3217 Fragments arranged by size: 3217 948 32 PstI C_TGCA'G Cuts at: 0 201 3013 3407 3772 3890 4197 Size: 201 2812 394 365 118 307 Fragments arranged by size: 2812 394 365 307 201 118 PvuII CAG'CTG Cuts at: 0 2194 3514 4197 Size: 2194 1320 683 Fragments arranged by size: 2194 1320 683 SacI G_AGCT'C Cuts at: 0 297 2034 4197 Size: 297 1737 2163 Fragments arranged by size: 2163 1737 297 SapI GCTCTTCn'nnn_ Cuts at: 0 1268 1961 2579 3524 3686 4197 Size: 1268 693 618 945 162 511 Fragments arranged by size: 1268 945 693 618 511 162 ScaI AGT'ACT Cuts at: 0 195 3944 4197 Size: 195 3749 253 Fragments arranged by size: 3749 253 195 SexAI A'CCwGG_T Cuts at: 0 3848 4197 Size: 3848 349 SfcI C'TryA_G Cuts at: 0 197 968 1960 3009 3403 3768 3886 4197 Size: 197 771 992 1049 394 365 118 311 Fragments arranged by size: 1049 992 771 394 365 311 197 118 SfoI GGC'GCC Cuts at: 0 1507 4197 Size: 1507 2690 SmaI CCC'GGG Cuts at: 0 2149 4197 Size: 2149 2048 SmlI C'TyrA_G Cuts at: 0 2239 4123 4197 Size: 2239 1884 74 Fragments arranged by size: 2239 1884 74 SphI G_CATG'C Cuts at: 0 3412 4197 Size: 3412 785 StuI AGG'CCT Cuts at: 0 754 813 4153 4197 Size: 754 59 3340 44 Fragments arranged by size: 3340 754 59 44 StyI C'CwwG_G Cuts at: 0 1247 1457 2802 3573 3608 4104 4197 Size: 1247 210 1345 771 35 496 93 Fragments arranged by size: 1345 1247 771 496 210 93 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 3854 4197 Size: 3854 343 TatI w'GTAC_w Cuts at: 0 193 1042 2525 2551 2742 3436 3942 4197 Size: 193 849 1483 26 191 694 506 255 Fragments arranged by size: 1483 849 694 506 255 193 191 26 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 524 556 3588 3620 4197 Size: 524 32 3032 32 577 Fragments arranged by size: 3032 577 524 32 32 XbaI T'CTAG_A Cuts at: 0 1290 2034 4197 Size: 1290 744 2163 Fragments arranged by size: 2163 1290 744 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3756 4197 Size: 3756 441 XmaI C'CCGG_G Cuts at: 0 2147 4197 Size: 2147 2050 XmnI GAAnn'nnTTC Cuts at: 0 1187 2255 4197 Size: 1187 1068 1942 Fragments arranged by size: 1942 1187 1068 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AleI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NsiI NspI PflMI PfoI PmlI PpuMI PsiI PsrI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI BciVI BglI Bme1580I BmgBI BplI BsaXI BsiWI BsmBI BsrFI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EciI EcoRV FseI FspI FspAI KpnI MluI NaeI NgoMIV NotI NruI PacI PasI PciI PmeI PpiI PshAI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI ZraI