################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BsaAI BsaHI BseAI BsiWI BsoBI BspDI BspEI BspHI BspLU11I BspMI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraIII EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FseI FspI Hin1I HindIII KasI KpnI KspI MluI MroI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflIII 1244 ACACGT A^C[GA][CT]GT AhdI 3487 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3691 AGCGCT AGC^GCT ApaI 3259 GGGCCC GGGCC^C Asp700I 4297 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3487 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2149 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BcnI 2820 CCGGG CC^[GC]GG BsaBI 1846 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1079 TCCGGT [AT]^CCGG[AT] BsiEI 479 CGATCG CG[GA][CT]^CG Bsp1407I 561 TGTACA T^GTACA BsrDI 4136 CATTGC GCAATG(2/0) BsrGI 561 TGTACA T^GTACA CpoI 1076 CGGTCCG CG^G[AT]CCG DrdI 196 GACCGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 3487 GACTTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3691 AGCGCT AGC^GCT HpaI 4177 GTTAAC GTT^AAC MamI 1846 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3541 CAATTG C^AATTG MunI 3541 CAATTG C^AATTG NaeI 3189 GCCGGC GCC^GGC NciI 2820 CCGGG CC^[GC]GG NdeI 1942 CATATG CA^TATG NgoMIV 3189 GCCGGC G^CCGGC NspI 2026 ACATGC [GA]CATG^[CT] PflFI 2149 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 479 CGATCG CGAT^CG RsrII 1076 CGGTCCG CG^G[AT]CCG ScaI 3529 AGTACT AGT^ACT SpeI 2047 ACTAGT A^CTAGT SspBI 561 TGTACA T^GTACA SspI 4276 AATATT AAT^ATT Tth111I 2149 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1651 TCTAGA T^CTAGA XmnI 4297 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BclI 1733 TGATCA T^GATCA 3740 TGATCA BglI 290 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2781 GCCAGACAGGC BglII 1884 AGATCT A^GATCT 3891 AGATCT BsaI 1966 GAGACC GGTCTC(1/5) 2738 GAGACC BsmBI 1691 CGTCTC CGTCTC(1/5) 2743 CGTCTC BsmI 3432 GCATTC GAATGC(1/-1) 4140 GCATTC BssSI 495 CACGAG CACGAG(-5/-1) 1066 CACGAG BstXI 329 CCATAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3408 CCATGGTAGTGG DraI 570 TTTAAA TTT^AAA 3156 TTTAAA EcoRV 670 GATATC GAT^ATC 3500 GATATC FbaI 1733 TGATCA T^GATCA 3740 TGATCA HaeII 2831 GGCGCT [GA]GCGC^[CT] 3691 AGCGCT HincII 2130 GTTGAC GT[CT]^[GA]AC 4177 GTTAAC HindII 2130 GTTGAC GT[CT]^[GA]AC 4177 GTTAAC NcoI 249 CCATGG C^CATGG 3408 CCATGG PpuMI 901 GGGTCCT [GA]G^G[AT]CC[CT] 3715 AGGTCCC SacI 181 GAGCTC GAGCT^C 1757 GAGCTC SapI 2886 GAAGAGC GCTCTTC(1/4) 1197 GCTCTTC TaqI 1208 TCGA T^CGA 1746 TCGA TthHB8I 1208 TCGA T^CGA 1746 TCGA XcmI 3399 CCAGGTCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3405 CCACCATGGTAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII