(Circular) (Six-base) MAPSORT of: pF1KA1209.seq Check: 3610 from: 1 to: 7445 pF1KA1209 7445 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AccI GT'mk_AC Cuts at: 4483 4483 Size: 7445 Acc65I G'GTAC_C Cuts at: 4461 4461 Size: 7445 AclI AA'CG_TT Cuts at: 7285 7285 Size: 7445 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 298 951 1413 1772 5270 5702 6055 6717 298 Size: 653 462 359 3498 432 353 662 1026 Fragments arranged by size: 3498 1026 662 653 462 432 359 353 AfeI AGC'GCT Cuts at: 3801 3801 Size: 7445 AflIII A'CryG_T Cuts at: 1352 4797 6597 6878 1352 Size: 3445 1800 281 1919 Fragments arranged by size: 3445 1919 1800 281 AhdI GACnn_n'nnGTC Cuts at: 3600 3600 Size: 7445 AleI CACnn'nnGTG Cuts at: 2989 2989 Size: 7445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 161 499 1408 4261 161 Size: 338 909 2853 3345 Fragments arranged by size: 3345 2853 909 338 AlwNI CAG_nnn'CTG Cuts at: 3706 3903 3906 4750 6188 3706 Size: 197 3 844 1438 4963 Fragments arranged by size: 4963 1438 844 197 3 ApaI G_GGCC'C Cuts at: 3371 3371 Size: 7445 ApaLI G'TGCA_C Cuts at: 6283 6283 Size: 7445 ApoI r'AATT_y Cuts at: 644 961 3443 4044 4408 4449 6798 7054 644 Size: 317 2482 601 364 41 2349 256 1035 Fragments arranged by size: 2482 2349 1035 601 364 317 256 41 AseI AT'TA_AT Cuts at: 20 20 Size: 7445 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7445 AvaI C'yCGr_G Cuts at: 4465 4465 Size: 7445 BamHI G'GATC_C Cuts at: 4470 4470 Size: 7445 BanI G'GyrC_C Cuts at: 191 576 2440 3379 4461 4875 5123 5158 191 Size: 385 1864 939 1082 414 248 35 2478 Fragments arranged by size: 2478 1864 1082 939 414 385 248 35 BanII G_rGCy'C Cuts at: 293 610 1869 2419 2525 3371 4459 5489 293 Size: 317 1259 550 106 846 1088 1030 2249 Fragments arranged by size: 2249 1259 1088 1030 846 550 317 106 BbeI G_GCGC'C Cuts at: 4879 5127 4879 Size: 248 7197 BbsI GAAGACnn'nnnn_ Cuts at: 286 1428 1729 1778 2840 3047 286 Size: 1142 301 49 1062 207 4684 Fragments arranged by size: 4684 1142 1062 301 207 49 BciVI GTATCCnnnnn_n' Cuts at: 5343 6399 7445 5343 Size: 1056 1046 5343 Fragments arranged by size: 5343 1056 1046 BclI T'GATC_A Cuts at: 1841 3848 4965 1841 Size: 2007 1117 4321 Fragments arranged by size: 4321 2007 1117 BglI GCCn_nnn'nGGC Cuts at: 404 2895 404 Size: 2491 4954 BglII A'GATC_T Cuts at: 1992 3999 4960 6997 1992 Size: 2007 961 2037 2440 Fragments arranged by size: 2440 2037 2007 961 BlpI GC'TnA_GC Cuts at: 1423 2306 2923 4557 1423 Size: 883 617 1634 4311 Fragments arranged by size: 4311 1634 883 617 Bme1580I G_kGCm'C Cuts at: 194 579 3371 3985 5070 5163 6287 194 Size: 385 2792 614 1085 93 1124 1352 Fragments arranged by size: 2792 1352 1124 1085 614 385 93 BmgBI CAC'GTC Cuts at: 2934 2934 Size: 7445 BmrI ACTGGGnnnn_n' Cuts at: 885 2726 3396 3822 4657 4738 5073 7217 885 Size: 1841 670 426 835 81 335 2144 1113 Fragments arranged by size: 2144 1841 1113 835 670 426 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2869 2993 5812 2869 Size: 124 2819 4502 Fragments arranged by size: 4502 2819 124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 466 1552 2064 2617 2638 3301 3307 466 Size: 1086 512 553 21 663 6 4604 Fragments arranged by size: 4604 1086 663 553 512 21 6 Bpu10I CC'TnA_GC Cuts at: 1559 2918 1559 Size: 1359 6086 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 254 1027 1282 3455 3618 4621 5500 6214 6512 Size: 773 255 2173 163 1003 879 714 298 Cuts at: 6512 254 Size: 1187 Fragments arranged by size: 2173 1187 1003 879 773 714 298 255 163 BsaI GGTCTCn'nnnn_ Cuts at: 2068 2840 7149 2068 Size: 772 4309 2364 Fragments arranged by size: 4309 2364 772 BsaAI yAC'GTr Cuts at: 4798 4798 Size: 7445 BsaBI GATnn'nnATC Cuts at: 6 1958 4959 6996 6 Size: 1952 3001 2037 455 Fragments arranged by size: 3001 2037 1952 455 BsaHI Gr'CG_yC Cuts at: 4876 5124 5826 7322 4876 Size: 248 702 1496 4999 Fragments arranged by size: 4999 1496 702 248 BsaWI w'CCGG_w Cuts at: 1187 4634 5155 5833 6244 6391 6665 1187 Size: 3447 521 678 411 147 274 1967 Fragments arranged by size: 3447 1967 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2124 2154 2124 Size: 30 7415 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 607 1977 2221 2512 2971 607 Size: 1370 244 291 459 5081 Fragments arranged by size: 5081 1370 459 291 244 BseYI C'CCAG_C Cuts at: 1484 1778 1915 2119 2541 3458 4870 5628 6293 Size: 294 137 204 422 917 1412 758 665 Cuts at: 6293 6701 1484 Size: 408 2228 Fragments arranged by size: 2228 1412 917 758 665 422 408 294 204 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1495 2574 2884 3730 1495 Size: 1079 310 846 5210 Fragments arranged by size: 5210 1079 846 310 BsiEI CG_ry'CG Cuts at: 104 590 4987 5033 6263 104 Size: 486 4397 46 1230 1286 Fragments arranged by size: 4397 1286 1230 486 46 BsiHKAI G_wGCw'C Cuts at: 293 1181 1869 2371 2584 2914 4459 5237 5427 Size: 888 688 502 213 330 1545 778 190 Cuts at: 5427 6287 293 Size: 860 1451 Fragments arranged by size: 1545 1451 888 860 778 688 502 330 213 190 BsmI GAATG_Cn' Cuts at: 3540 4248 3540 Size: 708 6737 BsmBI CGTCTCn'nnnn_ Cuts at: 1805 2857 1805 Size: 1052 6393 Bsp1286I G_dGCh'C Cuts at: 194 293 579 610 1181 1869 2371 2419 2525 Size: 99 286 31 571 688 502 48 106 Cuts at: 2525 2584 2914 3371 3985 4459 5070 5163 5237 Size: 59 330 457 614 474 611 93 74 Cuts at: 5237 5427 5489 6287 194 Size: 190 62 798 1352 Fragments arranged by size: 1352 798 688 614 611 571 502 474 457 330 286 190 106 99 93 74 62 59 48 31 BspHI T'CATG_A Cuts at: 5877 5877 Size: 7445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 4495 4709 5011 5392 4495 Size: 214 302 381 6548 Fragments arranged by size: 6548 381 302 214 BsrBI CCG'CTC Cuts at: 50 1299 1422 1858 5737 5791 7275 7440 50 Size: 1249 123 436 3879 54 1484 165 55 Fragments arranged by size: 3879 1484 1249 436 165 123 55 54 BsrDI GCAATG_nn' Cuts at: 4243 5357 4243 Size: 1114 6331 BsrFI r'CCGG_y Cuts at: 3080 3297 4281 5443 5624 3080 Size: 217 984 1162 181 4901 Fragments arranged by size: 4901 1162 984 217 181 BsrGI T'GTAC_A Cuts at: 669 669 Size: 7445 BssSI C'ACGA_G Cuts at: 603 1174 5716 6424 603 Size: 571 4542 708 1624 Fragments arranged by size: 4542 1624 708 571 BstAPI GCAn_nnn'nTGC Cuts at: 198 2135 3903 4822 198 Size: 1937 1768 919 2821 Fragments arranged by size: 2821 1937 1768 919 BstBI TT'CG_AA Cuts at: 5806 5806 Size: 7445 BstXI CCAn_nnnn'nTGG Cuts at: 444 3523 444 Size: 3079 4366 BstYI r'GATC_y Cuts at: 1 1771 1992 2529 3999 4470 4937 4960 5295 Size: 1770 221 537 1470 471 467 23 335 Cuts at: 5295 5541 5847 5859 5945 6997 1 Size: 246 306 12 86 1052 449 Fragments arranged by size: 1770 1470 1052 537 471 467 449 335 306 246 221 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1622 2626 3487 1622 Size: 1004 861 5580 Fragments arranged by size: 5580 1004 861 BtgI C'CryG_G Cuts at: 107 357 503 917 3516 107 Size: 250 146 414 2599 4036 Fragments arranged by size: 4036 2599 414 250 146 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5395 5576 5739 5395 Size: 181 163 7101 Fragments arranged by size: 7101 181 163 BtsI GCAGTG_nn' Cuts at: 559 2121 4688 559 Size: 1562 2567 3316 Fragments arranged by size: 3316 2567 1562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 899 934 4184 4219 899 Size: 35 3250 35 4125 Fragments arranged by size: 4125 3250 35 35 DraI TTT'AAA Cuts at: 680 3266 4444 680 Size: 2586 1178 3681 Fragments arranged by size: 3681 2586 1178 DrdI GACnn_nn'nnGTC Cuts at: 310 5151 6495 310 Size: 4841 1344 1260 Fragments arranged by size: 4841 1344 1260 EaeI y'GGCC_r Cuts at: 599 839 956 2403 3131 3299 5030 5204 5595 Size: 240 117 1447 728 168 1731 174 391 Cuts at: 5595 5622 6967 599 Size: 27 1345 1077 Fragments arranged by size: 1731 1447 1345 1077 728 391 240 174 168 117 27 EagI C'GGCC_G Cuts at: 5030 5030 Size: 7445 EarI CTCTTCn'nnn_ Cuts at: 127 348 866 1312 1318 1336 2989 3344 3683 Size: 221 518 446 6 18 1653 355 339 Cuts at: 3683 5468 5678 127 Size: 1785 210 1894 Fragments arranged by size: 1894 1785 1653 518 446 355 339 221 210 18 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 144 5653 6395 6541 7255 144 Size: 5509 742 146 714 334 Fragments arranged by size: 5509 742 714 334 146 EcoICRI GAG'CTC Cuts at: 291 1867 4457 291 Size: 1576 2590 3279 Fragments arranged by size: 3279 2590 1576 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 298 466 951 1413 1552 1772 2064 2617 2638 Size: 168 485 462 139 220 292 553 21 Cuts at: 2638 3301 3307 5270 5702 6055 6717 298 Size: 663 6 1963 432 353 662 1026 Fragments arranged by size: 1963 1026 663 662 553 485 462 432 353 292 220 168 139 21 6 EcoNI CCTnn'n_nnAGG Cuts at: 1084 1625 2147 3698 1084 Size: 541 522 1551 4831 Fragments arranged by size: 4831 1551 541 522 EcoO109I rG'GnC_Cy Cuts at: 1010 3367 3824 4584 1010 Size: 2357 457 760 3871 Fragments arranged by size: 3871 2357 760 457 EcoRI G'AATT_C Cuts at: 4449 4449 Size: 7445 EcoRV GAT'ATC Cuts at: 780 3610 780 Size: 2830 4615 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1594 5464 1594 Size: 3870 3575 FspI TGC'GCA Cuts at: 5226 5226 Size: 7445 HaeII r_GCGC'y Cuts at: 2943 3803 4879 5127 6357 7128 2943 Size: 860 1076 248 1230 771 3260 Fragments arranged by size: 3260 1230 1076 860 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1756 1788 1803 1835 1984 2016 2521 2553 2761 Size: 32 15 32 149 32 505 32 208 Cuts at: 2761 2793 3586 3618 5287 5317 5319 5349 1756 Size: 32 793 32 1669 30 2 30 3852 Fragments arranged by size: 3852 1669 793 505 208 149 32 32 32 32 32 32 30 30 15 2 HincII GTy'rAC Cuts at: 2240 4287 4484 2240 Size: 2047 197 5201 Fragments arranged by size: 5201 2047 197 HpaI GTT'AAC Cuts at: 4287 4287 Size: 7445 KasI G'GCGC_C Cuts at: 4875 5123 4875 Size: 248 7197 KpnI G_GTAC'C Cuts at: 4465 4465 Size: 7445 MfeI C'AATT_G Cuts at: 3649 3649 Size: 7445 MluI A'CGCG_T Cuts at: 6878 6878 Size: 7445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3766 4133 4139 6204 6388 3766 Size: 367 6 2065 184 4823 Fragments arranged by size: 4823 2065 367 184 6 MscI TGG'CCA Cuts at: 841 958 2405 3133 5206 841 Size: 117 1447 728 2073 3080 Fragments arranged by size: 3080 2073 1447 728 117 MslI CAynn'nnrTG Cuts at: 1154 1396 2264 2989 3521 5434 5561 1154 Size: 242 868 725 532 1913 127 3038 Fragments arranged by size: 3038 1913 868 725 532 242 127 MspA1I CmG'CkG Cuts at: 220 1035 1782 2045 3903 4235 4556 4870 5230 Size: 815 747 263 1858 332 321 314 360 Cuts at: 5230 6014 6259 220 Size: 784 245 1406 Fragments arranged by size: 1858 1406 815 784 747 360 332 321 314 263 245 NaeI GCC'GGC Cuts at: 3299 5626 3299 Size: 2327 5118 NarI GG'CG_CC Cuts at: 4876 5124 4876 Size: 248 7197 NcoI C'CATG_G Cuts at: 107 357 3516 107 Size: 250 3159 4036 Fragments arranged by size: 4036 3159 250 NdeI CA'TA_TG Cuts at: 2051 2051 Size: 7445 NgoMIV G'CCGG_C Cuts at: 3297 5624 3297 Size: 2327 5118 NspI r_CATG'y Cuts at: 2138 4498 4998 6601 2138 Size: 2360 500 1603 2982 Fragments arranged by size: 2982 2360 1603 500 PasI CC'CwG_GG Cuts at: 735 735 Size: 7445 PciI A'CATG_T Cuts at: 6597 6597 Size: 7445 PmeI GTTT'AAAC Cuts at: 4444 4444 Size: 7445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5891 5923 5891 Size: 32 7413 PpuMI rG'GwC_Cy Cuts at: 1010 3824 1010 Size: 2814 4631 PspOMI G'GGCC_C Cuts at: 3367 3367 Size: 7445 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 215 247 215 Size: 32 7413 PstI C_TGCA'G Cuts at: 488 1463 2180 4234 4492 5177 488 Size: 975 717 2054 258 685 2756 Fragments arranged by size: 2756 2054 975 717 685 258 PvuI CG_AT'CG Cuts at: 104 590 104 Size: 486 6959 PvuII CAG'CTG Cuts at: 220 1782 2045 3903 4870 5230 220 Size: 1562 263 1858 967 360 2435 Fragments arranged by size: 2435 1858 1562 967 360 263 RsrII CG'GwC_CG Cuts at: 1185 5640 1185 Size: 4455 2990 SacI G_AGCT'C Cuts at: 293 1869 4459 293 Size: 1576 2590 3279 Fragments arranged by size: 3279 2590 1576 SalI G'TCGA_C Cuts at: 4482 4482 Size: 7445 SapI GCTCTTCn'nnn_ Cuts at: 1312 2989 5468 5678 1312 Size: 1677 2479 210 3079 Fragments arranged by size: 3079 2479 1677 210 SbfI CC_TGCA'GG Cuts at: 4492 4492 Size: 7445 ScaI AGT'ACT Cuts at: 3639 6962 3639 Size: 3323 4122 SfcI C'TryA_G Cuts at: 33 484 1121 1459 2176 3549 4230 4488 5173 Size: 451 637 338 717 1373 681 258 685 Cuts at: 5173 6141 6332 33 Size: 968 191 1146 Fragments arranged by size: 1373 1146 968 717 685 681 637 451 338 258 191 SfoI GGC'GCC Cuts at: 4877 5125 4877 Size: 248 7197 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7445 SmaI CCC'GGG Cuts at: 4467 4467 Size: 7445 SmlI C'TyrA_G Cuts at: 269 1042 1261 3470 3597 4600 5515 6229 6491 Size: 773 219 2209 127 1003 915 714 262 Cuts at: 6491 269 Size: 1223 Fragments arranged by size: 2209 1223 1003 915 773 714 262 219 127 SpeI A'CTAG_T Cuts at: 67 2155 67 Size: 2088 5357 SphI G_CATG'C Cuts at: 4498 4998 4498 Size: 500 6945 SspI AAT'ATT Cuts at: 4386 4386 Size: 7445 StyI C'CwwG_G Cuts at: 107 357 910 3516 3779 4579 4933 107 Size: 250 553 2606 263 800 354 2619 Fragments arranged by size: 2619 2606 800 553 354 263 250 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 320 558 594 757 1900 5028 5161 5620 5829 Size: 238 36 163 1143 3128 133 459 209 Cuts at: 5829 320 Size: 1936 Fragments arranged by size: 3128 1936 1143 459 238 209 163 133 36 TatI w'GTAC_w Cuts at: 669 3230 3637 6960 669 Size: 2561 407 3323 1154 Fragments arranged by size: 3323 2561 1154 407 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 147 179 891 923 1730 1762 2124 2156 6962 Size: 32 712 32 807 32 362 32 4806 Cuts at: 6962 6994 147 Size: 32 598 Fragments arranged by size: 4806 807 712 598 362 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2260 5242 2260 Size: 2982 4463 XbaI T'CTAG_A Cuts at: 1759 4476 1759 Size: 2717 4728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3514 3520 4940 3514 Size: 6 1420 6019 Fragments arranged by size: 6019 1420 6 XmaI C'CCGG_G Cuts at: 4465 4465 Size: 7445 XmnI GAAnn'nnTTC Cuts at: 4409 4409 Size: 7445 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PmeI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AgeI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BspEI BssHII BstEII BstZ17I ClaI DraIII FseI FspAI HindIII NheI NotI NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI