Restriction map for
pF1KA1209
(Linear) (Six-base) MAPSORT of: KIAA1209.seq Check: 7498 from: 1 to: 4332
KIAA1209.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 843 1305 1664 4332
Size: 190 653 462 359 2668
Fragments arranged by size:
2668 653 462 359 190
AfeI AGC'GCT
Cuts at: 0 3693 4332
Size: 3693 639
AflIII A'CryG_T
Cuts at: 0 1244 4332
Size: 1244 3088
AhdI GACnn_n'nnGTC
Cuts at: 0 3492 4332
Size: 3492 840
AleI CACnn'nnGTG
Cuts at: 0 2881 4332
Size: 2881 1451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 391 1300 4153 4332
Size: 53 338 909 2853 179
Fragments arranged by size:
2853 909 338 179 53
AlwNI CAG_nnn'CTG
Cuts at: 0 3598 3795 3798 4332
Size: 3598 197 3 534
Fragments arranged by size:
3598 534 197 3
ApaI G_GGCC'C
Cuts at: 0 3263 4332
Size: 3263 1069
ApoI r'AATT_y
Cuts at: 0 536 853 3335 3936 4300 4332
Size: 536 317 2482 601 364 32
Fragments arranged by size:
2482 601 536 364 317 32
BanI G'GyrC_C
Cuts at: 0 83 468 2332 3271 4332
Size: 83 385 1864 939 1061
Fragments arranged by size:
1864 1061 939 385 83
BanII G_rGCy'C
Cuts at: 0 185 502 1761 2311 2417 3263 4332
Size: 185 317 1259 550 106 846 1069
Fragments arranged by size:
1259 1069 846 550 317 185 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 1320 1621 1670 2732 2939 4332
Size: 178 1142 301 49 1062 207 1393
Fragments arranged by size:
1393 1142 1062 301 207 178 49
BclI T'GATC_A
Cuts at: 0 1733 3740 4332
Size: 1733 2007 592
Fragments arranged by size:
2007 1733 592
BglI GCCn_nnn'nGGC
Cuts at: 0 296 2787 4332
Size: 296 2491 1545
Fragments arranged by size:
2491 1545 296
BglII A'GATC_T
Cuts at: 0 1884 3891 4332
Size: 1884 2007 441
Fragments arranged by size:
2007 1884 441
BlpI GC'TnA_GC
Cuts at: 0 1315 2198 2815 4332
Size: 1315 883 617 1517
Fragments arranged by size:
1517 1315 883 617
Bme1580I G_kGCm'C
Cuts at: 0 86 471 3263 3877 4332
Size: 86 385 2792 614 455
Fragments arranged by size:
2792 614 455 385 86
BmgBI CAC'GTC
Cuts at: 0 2826 4332
Size: 2826 1506
BmrI ACTGGGnnnn_n'
Cuts at: 0 777 2618 3288 3714 4332
Size: 777 1841 670 426 618
Fragments arranged by size:
1841 777 670 618 426
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2761 2885 4332
Size: 2761 124 1447
Fragments arranged by size:
2761 1447 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1444 1956 2509 2530 3193 3199 4332
Size: 358 1086 512 553 21 663 6 1133
Fragments arranged by size:
1133 1086 663 553 512 358 21 6
Bpu10I CC'TnA_GC
Cuts at: 0 1451 2810 4332
Size: 1451 1359 1522
Fragments arranged by size:
1522 1451 1359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 919 1174 3347 3510 4332
Size: 146 773 255 2173 163 822
Fragments arranged by size:
2173 822 773 255 163 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 1960 2732 4332
Size: 1960 772 1600
Fragments arranged by size:
1960 1600 772
BsaBI GATnn'nnATC
Cuts at: 0 1850 4332
Size: 1850 2482
BsaWI w'CCGG_w
Cuts at: 0 1079 4332
Size: 1079 3253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2016 2046 4332
Size: 2016 30 2286
Fragments arranged by size:
2286 2016 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 1869 2113 2404 2863 4332
Size: 499 1370 244 291 459 1469
Fragments arranged by size:
1469 1370 499 459 291 244
BseYI C'CCAG_C
Cuts at: 0 1376 1670 1807 2011 2433 3350 4332
Size: 1376 294 137 204 422 917 982
Fragments arranged by size:
1376 982 917 422 294 204 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 2466 2776 3622 4332
Size: 1387 1079 310 846 710
Fragments arranged by size:
1387 1079 846 710 310
BsiEI CG_ry'CG
Cuts at: 0 482 4332
Size: 482 3850
BsiHKAI G_wGCw'C
Cuts at: 0 185 1073 1761 2263 2476 2806 4332
Size: 185 888 688 502 213 330 1526
Fragments arranged by size:
1526 888 688 502 330 213 185
BsmI GAATG_Cn'
Cuts at: 0 3432 4140 4332
Size: 3432 708 192
Fragments arranged by size:
3432 708 192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1697 2749 4332
Size: 1697 1052 1583
Fragments arranged by size:
1697 1583 1052
Bsp1286I G_dGCh'C
Cuts at: 0 86 185 471 502 1073 1761 2263 2311
Size: 86 99 286 31 571 688 502 48
Cuts at: 2311 2417 2476 2806 3263 3877 4332
Size: 106 59 330 457 614 455
Fragments arranged by size:
688 614 571 502 457 455 330 286
106 99 86 59 48 31
BsrBI CCG'CTC
Cuts at: 0 1191 1314 1750 4332
Size: 1191 123 436 2582
Fragments arranged by size:
2582 1191 436 123
BsrDI GCAATG_nn'
Cuts at: 0 4135 4332
Size: 4135 197
BsrFI r'CCGG_y
Cuts at: 0 2972 3189 4173 4332
Size: 2972 217 984 159
Fragments arranged by size:
2972 984 217 159
BsrGI T'GTAC_A
Cuts at: 0 561 4332
Size: 561 3771
BssSI C'ACGA_G
Cuts at: 0 495 1066 4332
Size: 495 571 3266
Fragments arranged by size:
3266 571 495
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 2027 3795 4332
Size: 90 1937 1768 537
Fragments arranged by size:
1937 1768 537 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 3415 4332
Size: 336 3079 917
Fragments arranged by size:
3079 917 336
BstYI r'GATC_y
Cuts at: 0 1663 1884 2421 3891 4332
Size: 1663 221 537 1470 441
Fragments arranged by size:
1663 1470 537 441 221
Bsu36I CC'TnA_GG
Cuts at: 0 1514 2518 3379 4332
Size: 1514 1004 861 953
Fragments arranged by size:
1514 1004 953 861
BtgI C'CryG_G
Cuts at: 0 249 395 809 3408 4332
Size: 249 146 414 2599 924
Fragments arranged by size:
2599 924 414 249 146
BtsI GCAGTG_nn'
Cuts at: 0 451 2013 4332
Size: 451 1562 2319
Fragments arranged by size:
2319 1562 451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 791 826 4076 4111 4332
Size: 791 35 3250 35 221
Fragments arranged by size:
3250 791 221 35 35
DraI TTT'AAA
Cuts at: 0 572 3158 4332
Size: 572 2586 1174
Fragments arranged by size:
2586 1174 572
DrdI GACnn_nn'nnGTC
Cuts at: 0 202 4332
Size: 202 4130
EaeI y'GGCC_r
Cuts at: 0 491 731 848 2295 3023 3191 4332
Size: 491 240 117 1447 728 168 1141
Fragments arranged by size:
1447 1141 728 491 240 168 117
EarI CTCTTCn'nnn_
Cuts at: 0 19 240 758 1204 1210 1228 2881 3236
Size: 19 221 518 446 6 18 1653 355
Cuts at: 3236 3575 4332
Size: 339 757
Fragments arranged by size:
1653 757 518 446 355 339 221 19
18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 4332
Size: 36 4296
EcoICRI GAG'CTC
Cuts at: 0 183 1759 4332
Size: 183 1576 2573
Fragments arranged by size:
2573 1576 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 358 843 1305 1444 1664 1956 2509
Size: 190 168 485 462 139 220 292 553
Cuts at: 2509 2530 3193 3199 4332
Size: 21 663 6 1133
Fragments arranged by size:
1133 663 553 485 462 292 220 190
168 139 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 976 1517 2039 3590 4332
Size: 976 541 522 1551 742
Fragments arranged by size:
1551 976 742 541 522
EcoO109I rG'GnC_Cy
Cuts at: 0 902 3259 3716 4332
Size: 902 2357 457 616
Fragments arranged by size:
2357 902 616 457
EcoRV GAT'ATC
Cuts at: 0 672 3502 4332
Size: 672 2830 830
Fragments arranged by size:
2830 830 672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1486 4332
Size: 1486 2846
HaeII r_GCGC'y
Cuts at: 0 2835 3695 4332
Size: 2835 860 637
Fragments arranged by size:
2835 860 637
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1648 1680 1695 1727 1876 1908 2413 2445
Size: 1648 32 15 32 149 32 505 32
Cuts at: 2445 2653 2685 3478 3510 4332
Size: 208 32 793 32 822
Fragments arranged by size:
1648 822 793 505 208 149 32 32
32 32 32 32 15
HincII GTy'rAC
Cuts at: 0 2132 4179 4332
Size: 2132 2047 153
Fragments arranged by size:
2132 2047 153
HpaI GTT'AAC
Cuts at: 0 4179 4332
Size: 4179 153
MfeI C'AATT_G
Cuts at: 0 3541 4332
Size: 3541 791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3658 4025 4031 4332
Size: 3658 367 6 301
Fragments arranged by size:
3658 367 301 6
MscI TGG'CCA
Cuts at: 0 733 850 2297 3025 4332
Size: 733 117 1447 728 1307
Fragments arranged by size:
1447 1307 733 728 117
MslI CAynn'nnrTG
Cuts at: 0 1046 1288 2156 2881 3413 4332
Size: 1046 242 868 725 532 919
Fragments arranged by size:
1046 919 868 725 532 242
MspA1I CmG'CkG
Cuts at: 0 112 927 1674 1937 3795 4127 4332
Size: 112 815 747 263 1858 332 205
Fragments arranged by size:
1858 815 747 332 263 205 112
NaeI GCC'GGC
Cuts at: 0 3191 4332
Size: 3191 1141
NcoI C'CATG_G
Cuts at: 0 249 3408 4332
Size: 249 3159 924
Fragments arranged by size:
3159 924 249
NdeI CA'TA_TG
Cuts at: 0 1943 4332
Size: 1943 2389
NgoMIV G'CCGG_C
Cuts at: 0 3189 4332
Size: 3189 1143
NspI r_CATG'y
Cuts at: 0 2030 4332
Size: 2030 2302
PasI CC'CwG_GG
Cuts at: 0 627 4332
Size: 627 3705
PpuMI rG'GwC_Cy
Cuts at: 0 902 3716 4332
Size: 902 2814 616
Fragments arranged by size:
2814 902 616
PspOMI G'GGCC_C
Cuts at: 0 3259 4332
Size: 3259 1073
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 107 139 4332
Size: 107 32 4193
Fragments arranged by size:
4193 107 32
PstI C_TGCA'G
Cuts at: 0 380 1355 2072 4126 4332
Size: 380 975 717 2054 206
Fragments arranged by size:
2054 975 717 380 206
PvuI CG_AT'CG
Cuts at: 0 482 4332
Size: 482 3850
PvuII CAG'CTG
Cuts at: 0 112 1674 1937 3795 4332
Size: 112 1562 263 1858 537
Fragments arranged by size:
1858 1562 537 263 112
RsrII CG'GwC_CG
Cuts at: 0 1077 4332
Size: 1077 3255
SacI G_AGCT'C
Cuts at: 0 185 1761 4332
Size: 185 1576 2571
Fragments arranged by size:
2571 1576 185
SapI GCTCTTCn'nnn_
Cuts at: 0 1204 2881 4332
Size: 1204 1677 1451
Fragments arranged by size:
1677 1451 1204
ScaI AGT'ACT
Cuts at: 0 3531 4332
Size: 3531 801
SfcI C'TryA_G
Cuts at: 0 376 1013 1351 2068 3441 4122 4332
Size: 376 637 338 717 1373 681 210
Fragments arranged by size:
1373 717 681 637 376 338 210
SmlI C'TyrA_G
Cuts at: 0 161 934 1153 3362 3489 4332
Size: 161 773 219 2209 127 843
Fragments arranged by size:
2209 843 773 219 161 127
SpeI A'CTAG_T
Cuts at: 0 2047 4332
Size: 2047 2285
SspI AAT'ATT
Cuts at: 0 4278 4332
Size: 4278 54
StyI C'CwwG_G
Cuts at: 0 249 802 3408 3671 4332
Size: 249 553 2606 263 661
Fragments arranged by size:
2606 661 553 263 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 212 450 486 649 1792 4332
Size: 212 238 36 163 1143 2540
Fragments arranged by size:
2540 1143 238 212 163 36
TatI w'GTAC_w
Cuts at: 0 561 3122 3529 4332
Size: 561 2561 407 803
Fragments arranged by size:
2561 803 561 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 783 815 1622 1654 2016 2048
Size: 39 32 712 32 807 32 362 32
Cuts at: 2048 4332
Size: 2284
Fragments arranged by size:
2284 807 712 362 39 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2152 4332
Size: 2152 2180
XbaI T'CTAG_A
Cuts at: 0 1651 4332
Size: 1651 2681
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3406 3412 4332
Size: 3406 6 920
Fragments arranged by size:
3406 920 6
XmnI GAAnn'nnTTC
Cuts at: 0 4301 4332
Size: 4301 31
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI BanI BanII BbsI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HpaI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PasI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SapI ScaI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BmtI BsaAI BsaHI
BsiWI BspEI BspHI BspMI BssHII BstBI BstEII BstZ17I
BtgZI ClaI DraIII EagI EcoRI FseI FspI FspAI
HindIII KasI KpnI MluI NarI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI XhoI XmaI ZraI