Restriction map for pF1KA1207
##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

Acc65I      AgeI        AhdI        Aor51HI     AscI        
Asp718I     AspEI       AviII       AvrII       BcgI        
BlnI        BsaBI       BsiWI       BspDI       BspLU11I    
BssHII      BstAPI      ClaI        CpoI        Eam1105I    
Eco47III    EcoRV       FseI        FspI        HpaI        
KpnI        KspI        MamI        MluI        NheI        
NotI        NruI        PacI        PaeR7I      PinAI       
PmeI        PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SgrAI       SnaBI       SpeI        
Sse8387I    SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        2642  GACGTC               GACGT^C
AccIII       5772  TCCGGA               T^CCGGA
AclI         1491  AACGTT               AA^CGTT
AflIII       2111  ACACGT               A^C[GA][CT]GT
Alw44I       4173  GTGCAC               G^TGCAC
ApaLI        4173  GTGCAC               G^TGCAC
BbvCI        1099  GCTGAGG              CCTCAGC(-5/-2)
BglII        5506  AGATCT               A^GATCT
BlpI          526  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      526  GCTCAGC              GC^T[ACGT]AGC
BseAI        5772  TCCGGA               T^CCGGA
BsiEI        2843  CGGCCG               CG[GA][CT]^CG
BsmBI         996  GAGACG               CGTCTC(1/5)
BspEI        5772  TCCGGA               T^CCGGA
Bst1107I      167  GTATAC               GTA^TAC
BstBI        5630  TTCGAA               TT^CGAA
BstZ17I       167  GTATAC               GTA^TAC
CelII         526  GCTCAGC              GC^T[ACGT]AGC
DraI         3274  TTTAAA               TTT^AAA
DraIII       3254  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3122  CCATGG               C^C[GA][CT]GG
EagI         2843  CGGCCG               C^GGCCG
EclXI        2843  CGGCCG               C^GGCCG
Eco52I       2843  CGGCCG               C^GGCCG
EcoRI        2369  GAATTC               G^AATTC
MfeI          221  CAATTG               C^AATTG
MroI         5772  TCCGGA               T^CCGGA
MunI          221  CAATTG               C^AATTG
NcoI         3122  CCATGG               C^CATGG
NdeI         3485  CATATG               CA^TATG
NspV         5630  TTCGAA               TT^CGAA
PshAI        5713  GACTTGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1491  AACGTT               AA^CGTT
PstI         3797  CTGCAG               CTGCA^G
SacI         4690  GAGCTC               GAGCT^C
ScaI         4973  AGTACT               AGT^ACT
SfiI          494  GGCCCAAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         5630  TTCGAA               TT^CGAA
SphI         2312  GCATGC               GCATG^C
SspI         1149  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI          167  GTATAC               GT^[AC][GT]AC
             3406  GTCTAC              
AccII         604  CGCG                 CG^CG
             5878  CGCG                
AlwNI        3219  CAGACGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5203  CAGGGGCTG           
AocI         4379  CCTCAGG              CC^T[ACGT]AGG
             5193  CCTCAGG             
AseI          237  ATTAAT               AT^TAAT
             2280  ATTAAT              
AsnI          237  ATTAAT               AT^TAAT
             2280  ATTAAT              
BamHI          60  GGATCC               G^GATCC
             1185  GGATCC              
BbrPI        3552  CACGTG               CAC^GTG
             5221  CACGTG              
BciVI        2030  GGATAC               GTATCC(6/5)
             2457  GTATCC              
BglI          495  GCCCAAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3289  GCCCTTGGGGC         
BsaAI        3552  CACGTG               [CT]AC^GT[GA]
             5221  CACGTG              
BsaI         5013  GAGACC               GGTCTC(1/5)
             2183  GGTCTC              
BsgI         5146  CTGCAC               GTGCAG(16/14)
             3260  GTGCAG              
BsmI         2409  GAATGC               GAATGC(1/-1)
             4654  GAATGC              
BspHI         884  TCATGA               T^CATGA
             4073  TCATGA              
BsrFI         563  GCCGGC               [GA]^CCGG[CT]
             2841  GCCGGC              
BssSI        2103  CACGAG               CACGAG(-5/-1)
             1180  CTCGTG              
BstEII        277  GGTGACC              G^GT[ACGT]ACC
             1071  GGTGACC             
BstPI         277  GGTGACC              G^GT[ACGT]ACC
             1071  GGTGACC             
BstUI         604  CGCG                 CG^CG
             5878  CGCG                
Bsu36I       4379  CCTCAGG              CC^T[ACGT]AGG
             5193  CCTCAGG             
Cfr10I        563  GCCGGC               [GA]^CCGG[CT]
             2841  GCCGGC              
DrdI          472  GACAATGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5440  GACATCTACGTC        
Eco81I       4379  CCTCAGG              CC^T[ACGT]AGG
             5193  CCTCAGG             
EcoNI        2701  CCTCCAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4210  CCTTATGCAGG         
EcoO65I       277  GGTGACC              G^GT[ACGT]ACC
             1071  GGTGACC             
EcoT22I      2411  ATGCAT               ATGCA^T
             2933  ATGCAT              
HincII        745  GTCAAC               GT[CT]^[GA]AC
             2898  GTTGAC              
HindII        745  GTCAAC               GT[CT]^[GA]AC
             2898  GTTGAC              
HindIII      1657  AAGCTT               A^AGCTT
             2751  AAGCTT              
MvnI          604  CGCG                 CG^CG
             5878  CGCG                
NaeI          563  GCCGGC               GCC^GGC
             2841  GCCGGC              
NgoMIV        563  GCCGGC               G^CCGGC
             2841  GCCGGC              
NsiI         2411  ATGCAT               ATGCA^T
             2933  ATGCAT              
PmaCI        3552  CACGTG               CAC^GTG
             5221  CACGTG              
PmlI         3552  CACGTG               CAC^GTG
             5221  CACGTG              
PpuMI         266  AGGACCT              [GA]G^G[AT]CC[CT]
             4948  GGGTCCC             
PshBI         237  ATTAAT               AT^TAAT
             2280  ATTAAT              
RcaI          884  TCATGA               T^CATGA
             4073  TCATGA              
SmaI         2824  CCCGGG               CCC^GGG
             4886  CCCGGG              
StuI          649  AGGCCT               AGG^CCT
             3892  AGGCCT              
XmaCI        2824  CCCGGG               C^CCGGG
             4886  CCCGGG              
XmaI         2824  CCCGGG               C^CCGGG
             4886  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflII       
AluI        AlwI        ApaI        ApoI        Asp700I     
AspHI       AspI        AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvI        
BclI        BcnI        BfaI        BfrI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrBI       BsrDI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        SspBI       StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
XmnI        
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