Restriction map for pF1KA1207
 (Linear) (Six-base) MAPSORT of: KIAA1207.seq  Check: 26  from: 1  to: 6183



KIAA1207.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:35  ..

AatII G_ACGT'C

Cuts at:      0    2646    6183 
   Size:       2646    3537
 
AccI GT'mk_AC

Cuts at:      0     168    3407    6183 
   Size:        168    3239    2776

  Fragments arranged by size: 

                3239    2776     168
 
AclI AA'CG_TT

Cuts at:      0    1492    6183 
   Size:       1492    4691
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    1264    1280    2095    2315    2473    3230    5189 
   Size:        283     981      16     815     220     158     757    1959

Cuts at:   5189    5263    6183 
   Size:         74     920

  Fragments arranged by size: 

                1959     981     920     815     757     283     220     158
                  74      16
 
AflII C'TTAA_G

Cuts at:      0    2523    3936    4036    6183 
   Size:       2523    1413     100    2147

  Fragments arranged by size: 

                2523    2147    1413     100
 
AflIII A'CryG_T

Cuts at:      0    2111    6183 
   Size:       2111    4072
 
AleI CACnn'nnGTG

Cuts at:      0     671    2236    3358    6183 
   Size:        671    1565    1122    2825

  Fragments arranged by size: 

                2825    1565    1122     671
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     310    6183 
   Size:        310    5873
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3224    5208    6183 
   Size:       3224    1984     975

  Fragments arranged by size: 

                3224    1984     975
 
ApaI G_GGCC'C

Cuts at:      0     413     490     497    1905    5166    6183 
   Size:        413      77       7    1408    3261    1017

  Fragments arranged by size: 

                3261    1408    1017     413      77       7
 
ApaLI G'TGCA_C

Cuts at:      0    4173    6183 
   Size:       4173    2010
 
ApoI r'AATT_y

Cuts at:      0      46     835    2042    2369    2689    3178    3673    4366 
   Size:         46     789    1207     327     320     489     495     693

Cuts at:   4366    4468    4856    5125    5526    5626    6183 
   Size:        102     388     269     401     100     557

  Fragments arranged by size: 

                1207     789     693     557     495     489     401     388
                 327     320     269     102     100      46
 
AseI AT'TA_AT

Cuts at:      0     238    2281    6183 
   Size:        238    2043    3902

  Fragments arranged by size: 

                3902    2043     238
 
AvaI C'yCGr_G

Cuts at:      0    2824    4886    5158    6183 
   Size:       2824    2062     272    1025

  Fragments arranged by size: 

                2824    2062    1025     272
 
BamHI G'GATC_C

Cuts at:      0      60    1185    6183 
   Size:         60    1125    4998

  Fragments arranged by size: 

                4998    1125      60
 
BanI G'GyrC_C

Cuts at:      0    2487    3286    3749    3858    5872    6062    6183 
   Size:       2487     799     463     109    2014     190     121

  Fragments arranged by size: 

                2487    2014     799     463     190     121     109
 
BanII G_rGCy'C

Cuts at:      0     413     490     497    1667    1905    4567    4694    5166 
   Size:        413      77       7    1170     238    2662     127     472

Cuts at:   5166    6183 
   Size:       1017

  Fragments arranged by size: 

                2662    1170    1017     472     413     238     127      77
                   7
 
BbeI G_GCGC'C

Cuts at:      0    3862    5876    6066    6183 
   Size:       3862    2014     190     117

  Fragments arranged by size: 

                3862    2014     190     117
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     467    1645    2105    4048    4304    4769    6183 
   Size:        467    1178     460    1943     256     465    1414

  Fragments arranged by size: 

                1943    1414    1178     467     465     460     256
 
BbvCI CC'TCA_GC

Cuts at:      0    1100    6183 
   Size:       1100    5083
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2024    2468    6183 
   Size:       2024     444    3715

  Fragments arranged by size: 

                3715    2024     444
 
BclI T'GATC_A

Cuts at:      0    3003    4025    4121    5375    5648    6183 
   Size:       3003    1022      96    1254     273     535

  Fragments arranged by size: 

                3003    1254    1022     535     273      96
 
BfrBI ATG'CAT

Cuts at:      0    2413    2935    6183 
   Size:       2413     522    3248

  Fragments arranged by size: 

                3248    2413     522
 
BglI GCCn_nnn'nGGC

Cuts at:      0     501    3295    6183 
   Size:        501    2794    2888

  Fragments arranged by size: 

                2888    2794     501
 
BglII A'GATC_T

Cuts at:      0    5506    6183 
   Size:       5506     677
 
BlpI GC'TnA_GC

Cuts at:      0     527    6183 
   Size:        527    5656
 
Bme1580I G_kGCm'C

Cuts at:      0     413     490     497    1905    3291    4177    5166    6183 
   Size:        413      77       7    1408    1386     886     989    1017

  Fragments arranged by size: 

                1408    1386    1017     989     886     413      77       7
            
 
BmgBI CAC'GTC

Cuts at:      0     669    6183 
   Size:        669    5514
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     350    1341    1445    1947    3748    3974    4753    4792 
   Size:        350     991     104     502    1801     226     779      39

Cuts at:   4792    6183 
   Size:       1391

  Fragments arranged by size: 

                1801    1391     991     779     502     350     226     104
                  39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2894    3246    3352    4678    5006    5628    6183 
   Size:       2894     352     106    1326     328     622     555

  Fragments arranged by size: 

                2894    1326     622     555     352     328     106
 
Bpu10I CC'TnA_GC

Cuts at:      0     403    1100    5216    6183 
   Size:        403     697    4116     967

  Fragments arranged by size: 

                4116     967     697     403
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180    1654    1681    2665    4417    4823    6183 
   Size:        180    1474      27     984    1752     406    1360

  Fragments arranged by size: 

                1752    1474    1360     984     406     180      27
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2189    5007    6183 
   Size:       2189    2818    1176

  Fragments arranged by size: 

                2818    2189    1176
 
BsaAI yAC'GTr

Cuts at:      0    3554    5223    6183 
   Size:       3554    1669     960

  Fragments arranged by size: 

                3554    1669     960
 
BsaHI Gr'CG_yC

Cuts at:      0    2643    3859    5873    6063    6183 
   Size:       2643    1216    2014     190     120

  Fragments arranged by size: 

                2643    2014    1216     190     120
 
BsaWI w'CCGG_w

Cuts at:      0     973    1064    5772    6183 
   Size:        973      91    4708     411

  Fragments arranged by size: 

                4708     973     411      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2527    2557    2892    2922    2982    3012    3995    4025 
   Size:       2527      30     335      30      60      30     983      30

Cuts at:   4025    4087    4117    6183 
   Size:         62      30    2066

  Fragments arranged by size: 

                2527    2066     983     335      62      60      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     446     977    3243    4016    4984    6121    6183 
   Size:        446     531    2266     773     968    1137      62

  Fragments arranged by size: 

                2266    1137     968     773     531     446      62
 
BseYI C'CCAG_C

Cuts at:      0     412    2711    2890    2975    3149    4597    5887    6183 
   Size:        412    2299     179      85     174    1448    1290     296

  Fragments arranged by size: 

                2299    1448    1290     412     296     179     174      85
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3281    5131    6183 
   Size:       3281    1850    1052

  Fragments arranged by size: 

                3281    1850    1052
 
BsiEI CG_ry'CG

Cuts at:      0    2846    6183 
   Size:       2846    3337
 
BsiHKAI G_wGCw'C

Cuts at:      0    4177    4694    5222    6131    6183 
   Size:       4177     517     528     909      52

  Fragments arranged by size: 

                4177     909     528     517      52
 
BsmI GAATG_Cn'

Cuts at:      0    2415    4660    6183 
   Size:       2415    2245    1523

  Fragments arranged by size: 

                2415    2245    1523
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     990    6183 
   Size:        990    5193
 
Bsp1286I G_dGCh'C

Cuts at:      0     413     490     497    1667    1905    3291    4177    4567 
   Size:        413      77       7    1170     238    1386     886     390

Cuts at:   4567    4694    5166    5222    6131    6183 
   Size:        127     472      56     909      52

  Fragments arranged by size: 

                1386    1170     909     886     472     413     390     238
                 127      77      56      52       7
 
BspEI T'CCGG_A

Cuts at:      0    5772    6183 
   Size:       5772     411
 
BspHI T'CATG_A

Cuts at:      0     884    4073    6183 
   Size:        884    3189    2110

  Fragments arranged by size: 

                3189    2110     884
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1691    2764    5375    5747    6183 
   Size:       1691    1073    2611     372     436

  Fragments arranged by size: 

                2611    1691    1073     436     372
 
BsrBI CCG'CTC

Cuts at:      0     407    2959    5175    6183 
   Size:        407    2552    2216    1008

  Fragments arranged by size: 

                2552    2216    1008     407
 
BsrDI GCAATG_nn'

Cuts at:      0    1055    1167    5475    6183 
   Size:       1055     112    4308     708

  Fragments arranged by size: 

                4308    1055     708     112
 
BsrFI r'CCGG_y

Cuts at:      0     563    2841    6183 
   Size:        563    2278    3342

  Fragments arranged by size: 

                3342    2278     563
 
BsrGI T'GTAC_A

Cuts at:      0    1225    3869    4103    6183 
   Size:       1225    2644     234    2080

  Fragments arranged by size: 

                2644    2080    1225     234
 
BssSI C'ACGA_G

Cuts at:      0    1180    2103    6183 
   Size:       1180     923    4080

  Fragments arranged by size: 

                4080    1180     923
 
BstBI TT'CG_AA

Cuts at:      0    5631    6183 
   Size:       5631     552
 
BstEII G'GTnAC_C

Cuts at:      0     277    1071    6183 
   Size:        277     794    5112

  Fragments arranged by size: 

                5112     794     277
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     357    2711    5310    6183 
   Size:        357    2354    2599     873

  Fragments arranged by size: 

                2599    2354     873     357
 
BstYI r'GATC_y

Cuts at:      0      60     289     599    1185    1607    2162    3480    4536 
   Size:         60     229     310     586     422     555    1318    1056

Cuts at:   4536    4574    5506    6183 
   Size:         38     932     677

  Fragments arranged by size: 

                1318    1056     932     677     586     555     422     310
                 229      60      38
 
BstZ17I GTA'TAC

Cuts at:      0     169    6183 
   Size:        169    6014
 
Bsu36I CC'TnA_GG

Cuts at:      0    4380    5194    6183 
   Size:       4380     814     989

  Fragments arranged by size: 

                4380     989     814
 
BtgI C'CryG_G

Cuts at:      0    3122    6183 
   Size:       3122    3061
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1939    6183 
   Size:       1939    4244
 
BtsI GCAGTG_nn'

Cuts at:      0    3908    4953    6183 
   Size:       3908    1045    1230

  Fragments arranged by size: 

                3908    1230    1045
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1614    1649    6183 
   Size:       1614      35    4534

  Fragments arranged by size: 

                4534    1614      35
 
DraI TTT'AAA

Cuts at:      0    3276    6183 
   Size:       3276    2907
 
DraIII CAC_nnn'GTG

Cuts at:      0    3259    6183 
   Size:       3259    2924
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     478    5446    6183 
   Size:        478    4968     737

  Fragments arranged by size: 

                4968     737     478
 
EaeI y'GGCC_r

Cuts at:      0     675     873    2843    4167    5848    6122    6183 
   Size:        675     198    1970    1324    1681     274      61

  Fragments arranged by size: 

                1970    1681    1324     675     274     198      61
 
EagI C'GGCC_G

Cuts at:      0    2843    6183 
   Size:       2843    3340
 
EarI CTCTTCn'nnn_

Cuts at:      0    2291    2344    3319    4075    5165    5189    6121    6183 
   Size:       2291      53     975     756    1090      24     932      62

  Fragments arranged by size: 

                2291    1090     975     932     756      62      53      24
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     365     582    1728    3202    4167    6183 
   Size:        365     217    1146    1474     965    2016

  Fragments arranged by size: 

                2016    1474    1146     965     365     217
 
EcoICRI GAG'CTC

Cuts at:      0    4692    6183 
   Size:       4692    1491
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    1264    1280    2095    2315    2473    2894    3230 
   Size:        283     981      16     815     220     158     421     336

Cuts at:   3230    3246    3352    4678    5006    5189    5263    5628    6183 
   Size:         16     106    1326     328     183      74     365     555

  Fragments arranged by size: 

                1326     981     815     555     421     365     336     328
                 283     220     183     158     106      74      16      16
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2705    4214    6183 
   Size:       2705    1509    1969

  Fragments arranged by size: 

                2705    1969    1509
 
EcoO109I rG'GnC_Cy

Cuts at:      0     267     486     487    4476    4949    5162    5163    5323 
   Size:        267     219       1    3989     473     213       1     160

Cuts at:   5323    6183 
   Size:        860

  Fragments arranged by size: 

                3989     860     473     267     219     213     160       1
                   1
 
EcoRI G'AATT_C

Cuts at:      0    2369    6183 
   Size:       2369    3814
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     547    3307    3489    4270    4584    5194    6006    6183 
   Size:        547    2760     182     781     314     610     812     177

  Fragments arranged by size: 

                2760     812     781     610     547     314     182     177
            
 
HaeII r_GCGC'y

Cuts at:      0    3862    4188    5876    6066    6183 
   Size:       3862     326    1688     190     117

  Fragments arranged by size: 

                3862    1688     326     190     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201     987    1019    1114    1146    1408    1440 
   Size:        169      32     786      32      95      32     262      32

Cuts at:   1440    1592    1624    2227    2259    2398    2430    2495    2527 
   Size:        152      32     603      32     139      32      65      32

Cuts at:   2527    2724    2756    2892    2923    2924    2955    3395    3427 
   Size:        197      32     136      31       1      31     440      32

Cuts at:   3427    4566    4598    5815    5847    6183 
   Size:       1139      32    1217      32     336

  Fragments arranged by size: 

                1217    1139     786     603     440     336     262     197
                 169     152     139     136      95      65      32      32
                  32      32      32      32      32      32      32      32
                  32      32      31      31       1
 
HincII GTy'rAC

Cuts at:      0     747    2900    6183 
   Size:        747    2153    3283

  Fragments arranged by size: 

                3283    2153     747
 
HindIII A'AGCT_T

Cuts at:      0    1657    2751    6183 
   Size:       1657    1094    3432

  Fragments arranged by size: 

                3432    1657    1094
 
KasI G'GCGC_C

Cuts at:      0    3858    5872    6062    6183 
   Size:       3858    2014     190     121

  Fragments arranged by size: 

                3858    2014     190     121
 
MfeI C'AATT_G

Cuts at:      0     221    6183 
   Size:        221    5962
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     500     831    1341    1513    2204    3854    4502 
   Size:        449      51     331     510     172     691    1650     648

Cuts at:   4502    4530    5741    6183 
   Size:         28    1211     442

  Fragments arranged by size: 

                1650    1211     691     648     510     449     442     331
                 172      51      28
 
MscI TGG'CCA

Cuts at:      0     677     875    5850    6183 
   Size:        677     198    4975     333

  Fragments arranged by size: 

                4975     677     333     198
 
MslI CAynn'nnrTG

Cuts at:      0     395     671    2236    2332    3358    4078    4653    6183 
   Size:        395     276    1565      96    1026     720     575    1530

  Fragments arranged by size: 

                1565    1530    1026     720     575     395     276      96
            
 
MspA1I CmG'CkG

Cuts at:      0    2058    2289    3265    5025    6183 
   Size:       2058     231     976    1760    1158

  Fragments arranged by size: 

                2058    1760    1158     976     231
 
NaeI GCC'GGC

Cuts at:      0     565    2843    6183 
   Size:        565    2278    3340

  Fragments arranged by size: 

                3340    2278     565
 
NarI GG'CG_CC

Cuts at:      0    3859    5873    6063    6183 
   Size:       3859    2014     190     120

  Fragments arranged by size: 

                3859    2014     190     120
 
NcoI C'CATG_G

Cuts at:      0    3122    6183 
   Size:       3122    3061
 
NdeI CA'TA_TG

Cuts at:      0    3486    6183 
   Size:       3486    2697
 
NgoMIV G'CCGG_C

Cuts at:      0     563    2841    6183 
   Size:        563    2278    3342

  Fragments arranged by size: 

                3342    2278     563
 
NsiI A_TGCA'T

Cuts at:      0    2415    2937    6183 
   Size:       2415     522    3246

  Fragments arranged by size: 

                3246    2415     522
 
NspI r_CATG'y

Cuts at:      0     960    2316    4110    6183 
   Size:        960    1356    1794    2073

  Fragments arranged by size: 

                2073    1794    1356     960
 
PasI CC'CwG_GG

Cuts at:      0     490    3882    5268    6183 
   Size:        490    3392    1386     915

  Fragments arranged by size: 

                3392    1386     915     490
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1495    3020    4804    6183 
   Size:       1495    1525    1784    1379

  Fragments arranged by size: 

                1784    1525    1495    1379
 
PmlI CAC'GTG

Cuts at:      0    3554    5223    6183 
   Size:       3554    1669     960

  Fragments arranged by size: 

                3554    1669     960
 
PpuMI rG'GwC_Cy

Cuts at:      0     267    4949    6183 
   Size:        267    4682    1234

  Fragments arranged by size: 

                4682    1234     267
 
PshAI GACnn'nnGTC

Cuts at:      0    5717    6183 
   Size:       5717     466
 
PsiI TTA'TAA

Cuts at:      0     797    6183 
   Size:        797    5386
 
PspOMI G'GGCC_C

Cuts at:      0     409     486     493    1901    5162    6183 
   Size:        409      77       7    1408    3261    1021

  Fragments arranged by size: 

                3261    1408    1021     409      77       7
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1469    1501    6183 
   Size:       1469      32    4682

  Fragments arranged by size: 

                4682    1469      32
 
PstI C_TGCA'G

Cuts at:      0    3801    6183 
   Size:       3801    2382
 
PvuII CAG'CTG

Cuts at:      0    2058    2289    3265    5025    6183 
   Size:       2058     231     976    1760    1158

  Fragments arranged by size: 

                2058    1760    1158     976     231
 
SacI G_AGCT'C

Cuts at:      0    4694    6183 
   Size:       4694    1489
 
SanDI GG'GwC_CC

Cuts at:      0    4949    6183 
   Size:       4949    1234
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2291    3319    5165    5189    6121    6183 
   Size:       2291    1028    1846      24     932      62

  Fragments arranged by size: 

                2291    1846    1028     932      62      24
 
ScaI AGT'ACT

Cuts at:      0    4975    6183 
   Size:       4975    1208
 
SfcI C'TryA_G

Cuts at:      0     254    1596    3613    3797    5112    6183 
   Size:        254    1342    2017     184    1315    1071

  Fragments arranged by size: 

                2017    1342    1315    1071     254     184
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     501    6183 
   Size:        501    5682
 
SfoI GGC'GCC

Cuts at:      0    3860    5874    6064    6183 
   Size:       3860    2014     190     119

  Fragments arranged by size: 

                3860    2014     190     119
 
SmaI CCC'GGG

Cuts at:      0    2826    4888    6183 
   Size:       2826    2062    1295

  Fragments arranged by size: 

                2826    2062    1295
 
SmlI C'TyrA_G

Cuts at:      0     159    1633    1660    2523    2680    3936    4036    4432 
   Size:        159    1474      27     863     157    1256     100     396

Cuts at:   4432    4838    6183 
   Size:        406    1345

  Fragments arranged by size: 

                1474    1345    1256     863     406     396     159     157
                 100      27
 
SphI G_CATG'C

Cuts at:      0    2316    6183 
   Size:       2316    3867
 
SspI AAT'ATT

Cuts at:      0    1151    6183 
   Size:       1151    5032
 
StuI AGG'CCT

Cuts at:      0     651    3894    6183 
   Size:        651    3243    2289

  Fragments arranged by size: 

                3243    2289     651
 
StyI C'CwwG_G

Cuts at:      0     192     497    1897    3122    3291    4091    5390    6183 
   Size:        192     305    1400    1225     169     800    1299     793

  Fragments arranged by size: 

                1400    1299    1225     800     793     305     192     169
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1059    2904    3639    3750    3767    4062    4781    5277 
   Size:       1059    1845     735     111      17     295     719     496

Cuts at:   5277    5655    6183 
   Size:        378     528

  Fragments arranged by size: 

                1845    1059     735     719     528     496     378     295
                 111      17
 
TatI w'GTAC_w

Cuts at:      0    1225    1535    1718    1853    2390    3869    4103    4973 
   Size:       1225     310     183     135     537    1479     234     870

Cuts at:   4973    6183 
   Size:       1210

  Fragments arranged by size: 

                1479    1225    1210     870     537     310     234     183
                 135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     578     610    2713    2745    2982    3014    3995    4027 
   Size:        578      32    2103      32     237      32     981      32

Cuts at:   4027    5051    5083    5715    5747    6183 
   Size:       1024      32     632      32     436

  Fragments arranged by size: 

                2103    1024     981     632     578     436     237      32
                  32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     516    3403    4231    4642    6183 
   Size:        516    2887     828     411    1541

  Fragments arranged by size: 

                2887    1541     828     516     411
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     354    2918    3450    3633    3987    4144    4185    4311 
   Size:        354    2564     532     183     354     157      41     126

Cuts at:   4311    4708    6183 
   Size:        397    1475

  Fragments arranged by size: 

                2564    1475     532     397     354     354     183     157
                 126      41
 
XmaI C'CCGG_G

Cuts at:      0    2824    4886    6183 
   Size:       2824    2062    1297

  Fragments arranged by size: 

                2824    2062    1297
 
XmnI GAAnn'nnTTC

Cuts at:      0    2693    3952    4081    6183 
   Size:       2693    1259     129    2102

  Fragments arranged by size: 

                2693    2102    1259     129
 
ZraI GAC'GTC

Cuts at:      0    2644    6183 
   Size:       2644    3539
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI    AflII   AflIII     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII  HindIII
     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NsiI     NspI     PasI    PflMI     PmlI
    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI    PvuII     SacI
    SanDI     SapI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI     AgeI     AhdI     AloI     AscI    AsiSI
    AvrII     BaeI     BcgI     BmtI     BplI    BsaBI    BsiWI   BssHII
   BstAPI     ClaI    EcoRV     FseI     FspI    FspAI     HpaI     KpnI
     MluI     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI
     PpiI    PspXI     PvuI    RsrII    SacII     SalI     SbfI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI     XbaI     XhoI
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