################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AclI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BbrPI BbsI BcgI BciVI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiWI BsmBI BsmI BspDI BspLU11I BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI MaeII MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SapI SbfI ScaI SfiI SnaBI SphI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccI 211 GTCGAC GT^[AC][GT]AC AccII 394 CGCG CG^CG AccIII 383 TCCGGA T^CCGGA AflII 1639 CTTAAG C^TTAAG AocI 1668 CCTGAGG CC^T[ACGT]AGG Asp700I 447 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 864 TGGCCA TGG^CCA BbeI 43 GGCGCC GGCGC^C BbvCI 661 GCTGAGG CCTCAGC(-5/-2) BclI 125 TGATCA T^GATCA BfrI 1639 CTTAAG C^TTAAG BsaWI 383 TCCGGA [AT]^CCGG[AT] BseAI 383 TCCGGA T^CCGGA BseRI 1724 CTCCTC GAGGAG(10/8) BsgI 821 CTGCAC GTGCAG(16/14) Bsp1407I 326 TGTACA T^GTACA BspEI 383 TCCGGA T^CCGGA BspHI 1728 TCATGA T^CATGA BsrBI 1105 GAGCGG CCGCTC(-3/-3) BsrFI 46 GCCGGC [GA]^CCGG[CT] BsrGI 326 TGTACA T^GTACA BstBI 454 TTCGAA TT^CGAA BstUI 394 CGCG CG^CG BstXI 926 CCAATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1668 CCTGAGG CC^T[ACGT]AGG Cfr10I 46 GCCGGC [GA]^CCGG[CT] DraII 541 AGGCCCC [GA]G^G[ACGT]CC[CT] Eco81I 1668 CCTGAGG CC^T[ACGT]AGG EcoNI 1554 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 541 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 1691 GAATTC G^AATTC FbaI 125 TGATCA T^GATCA HgaI 50 GCGTC GACGC(5/10) HindIII 688 AAGCTT A^AGCTT HpaI 520 GTTAAC GTT^AAC KasI 43 GGCGCC G^GCGCC KspI 393 CCGCGG CCGC^GG MluNI 864 TGGCCA TGG^CCA MroI 383 TCCGGA T^CCGGA MscI 864 TGGCCA TGG^CCA MvnI 394 CGCG CG^CG NaeI 46 GCCGGC GCC^GGC NarI 43 GGCGCC GG^CGCC NcoI 1001 CCATGG C^CATGG NgoMIV 46 GCCGGC G^CCGGC NspI 215 ACATGC [GA]CATG^[CT] NspV 454 TTCGAA TT^CGAA PflMI 871 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1728 TCATGA T^CATGA SacI 1468 GAGCTC GAGCT^C SacII 393 CCGCGG CCGC^GG SalI 211 GTCGAC G^TCGAC SexAI 302 ACCTGGT A^CC[AT]GGT SfoI 43 GGCGCC GGC^GCC SfuI 454 TTCGAA TT^CGAA SgrAI 45 CGCCGGCG C[GA]^CCGG[CT]G SpeI 1654 ACTAGT A^CTAGT SspBI 326 TGTACA T^GTACA StuI 606 AGGCCT AGG^CCT Van91I 871 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1140 TCTAGA T^CTAGA XcmI 867 CCAGCCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 447 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 1087 GGTACC G^GTACC 1245 GGTACC AcyI 43 GGCGCC G[GA]^CG[CT]C 49 GGCGTC Asp718I 1087 GGTACC G^GTACC 1245 GGTACC AspHI 884 GTGCTC G[AT]GC[AT]^C 1468 GAGCTC AvaI 54 CCCGGG C^[CT]CG[GA]G 415 CCCGGG BamHI 386 GGATCC G^GATCC 1109 GGATCC BanII 100 GAGCCC G[GA]GC[CT]^C 1468 GAGCTC BglI 866 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1039 GCCAACATGGC BsaHI 43 GGCGCC G[GA]^CG[CT]C 49 GGCGTC BsiHKAI 884 GTGCTC G[AT]GC[AT]^C 1468 GAGCTC BsoBI 54 CCCGGG C^[CT]CG[GA]G 415 CCCGGG BspMI 917 ACCTGC ACCTGC(4/8) 378 GCAGGT BsrDI 1310 CATTGC GCAATG(2/0) 1006 GCAATG BstAPI 991 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1305 GCAGGCATTGC DraIII 301 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 319 CACACTGTG EaeI 252 CGGCCA [CT]^GGCC[GA] 864 TGGCCA Hin1I 43 GGCGCC G[GA]^CG[CT]C 49 GGCGTC KpnI 1087 GGTACC GGTAC^C 1245 GGTACC MslI 1002 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1729 CATGATAGTG PvuII 647 CAGCTG CAG^CTG 1291 CAGCTG SmaI 54 CCCGGG CCC^GGG 415 CCCGGG SmlI 295 CTCAAG C^T[CT][GA]AG 1639 CTTAAG Tsp45I 1272 GTCAC ^GT[GC]AC 1658 GTGAC XmaCI 54 CCCGGG C^CCGGG 415 CCCGGG XmaI 54 CCCGGG C^CCGGG 415 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanI BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII