(Circular) (Six-base) MAPSORT of: pF1KB7820.seq Check: 2279 from: 1 to: 4910 pF1KB7820 4910 bp With 182 enzymes: SgfI * October 7, 2009 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1034 1034 Size: 4910 AccI GT'mk_AC Cuts at: 320 1948 320 Size: 1628 3282 Acc65I G'GTAC_C Cuts at: 1195 1353 1926 1195 Size: 158 573 4179 Fragments arranged by size: 4179 573 158 AclI AA'CG_TT Cuts at: 4750 4750 Size: 4910 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 466 769 802 1684 2735 3167 3914 4182 466 Size: 303 33 882 1051 432 747 268 1194 Fragments arranged by size: 1194 1051 882 747 432 303 268 33 AflII C'TTAA_G Cuts at: 1747 1747 Size: 4910 AflIII A'CryG_T Cuts at: 2262 3366 4343 2262 Size: 1104 977 2829 Fragments arranged by size: 2829 1104 977 AgeI A'CCGG_T Cuts at: 3298 3298 Size: 4910 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 701 701 Size: 4910 AlwNI CAG_nnn'CTG Cuts at: 369 856 1500 2215 3782 369 Size: 487 644 715 1567 1497 Fragments arranged by size: 1567 1497 715 644 487 ApaLI G'TGCA_C Cuts at: 3680 3680 Size: 4910 ApoI r'AATT_y Cuts at: 1364 1708 1799 1914 4263 4519 1364 Size: 344 91 115 2349 256 1755 Fragments arranged by size: 2349 1755 344 256 115 91 AseI AT'TA_AT Cuts at: 20 20 Size: 4910 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4910 AvaI C'yCGr_G Cuts at: 162 523 1930 162 Size: 361 1407 3142 Fragments arranged by size: 3142 1407 361 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1187 1220 1187 Size: 33 4877 BamHI G'GATC_C Cuts at: 494 1217 1935 494 Size: 723 718 3469 Fragments arranged by size: 3469 723 718 BanI G'GyrC_C Cuts at: 151 1195 1353 1926 2340 2588 2623 151 Size: 1044 158 573 414 248 35 2438 Fragments arranged by size: 2438 1044 573 414 248 158 35 BanII G_rGCy'C Cuts at: 212 1580 1924 2954 212 Size: 1368 344 1030 2168 Fragments arranged by size: 2168 1368 1030 344 BbeI G_GCGC'C Cuts at: 155 2344 2592 155 Size: 2189 248 2473 Fragments arranged by size: 2473 2189 248 BbvCI CC'TCA_GC Cuts at: 770 770 Size: 4910 BciVI GTATCCnnnnn_n' Cuts at: 2808 3569 4910 2808 Size: 761 1341 2808 Fragments arranged by size: 2808 1341 761 BclI T'GATC_A Cuts at: 233 2430 233 Size: 2197 2713 BglI GCCn_nnn'nGGC Cuts at: 980 1153 980 Size: 173 4737 BglII A'GATC_T Cuts at: 2425 4462 2425 Size: 2037 2873 BlpI GC'TnA_GC Cuts at: 2022 2022 Size: 4910 Bme1580I G_kGCm'C Cuts at: 2535 2628 3684 2535 Size: 93 1056 3761 Fragments arranged by size: 3761 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 2122 2203 2538 4682 2122 Size: 81 335 2144 2350 Fragments arranged by size: 2350 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1250 3277 1250 Size: 2027 2883 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 110 1170 1594 110 Size: 1060 424 3426 Fragments arranged by size: 3426 1060 424 Bpu10I CC'TnA_GC Cuts at: 656 770 656 Size: 114 4796 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 388 2086 2965 3457 3755 388 Size: 1698 879 492 298 1543 Fragments arranged by size: 1698 1543 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4614 4614 Size: 4910 BsaAI yAC'GTr Cuts at: 2263 2263 Size: 4910 BsaBI GATnn'nnATC Cuts at: 6 2424 4461 6 Size: 2418 2037 455 Fragments arranged by size: 2418 2037 455 BsaHI Gr'CG_yC Cuts at: 152 158 2341 2589 3291 4787 152 Size: 6 2183 248 702 1496 275 Fragments arranged by size: 2183 1496 702 275 248 6 BsaWI w'CCGG_w Cuts at: 491 2099 2620 3298 3572 3719 4130 491 Size: 1608 521 678 274 147 411 1271 Fragments arranged by size: 1608 1271 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1465 1495 1465 Size: 30 4880 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1823 1823 Size: 4910 BseYI C'CCAG_C Cuts at: 507 2335 3093 3670 4166 507 Size: 1828 758 577 496 1251 Fragments arranged by size: 1828 1251 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 914 914 Size: 4910 BsiEI CG_ry'CG Cuts at: 104 2452 2498 3706 104 Size: 2348 46 1208 1308 Fragments arranged by size: 2348 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 996 1580 1924 2702 2892 3684 996 Size: 584 344 778 190 792 2222 Fragments arranged by size: 2222 792 778 584 344 190 Bsp1286I G_dGCh'C Cuts at: 212 996 1580 1924 2535 2628 2702 2892 2954 Size: 784 584 344 611 93 74 190 62 Cuts at: 2954 3684 212 Size: 730 1438 Fragments arranged by size: 1438 784 730 611 584 344 190 93 74 62 BspEI T'CCGG_A Cuts at: 491 4130 491 Size: 3639 1271 BspHI T'CATG_A Cuts at: 1836 4086 1836 Size: 2250 2660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 477 1034 1960 2174 2476 2857 477 Size: 557 926 214 302 381 2530 Fragments arranged by size: 2530 926 557 381 302 214 BsrBI CCG'CTC Cuts at: 50 1215 3202 3256 4740 4905 50 Size: 1165 1987 54 1484 165 55 Fragments arranged by size: 1987 1484 1165 165 55 54 BsrDI GCAATG_nn' Cuts at: 1121 1417 2822 1121 Size: 296 1405 3209 Fragments arranged by size: 3209 1405 296 BsrFI r'CCGG_y Cuts at: 154 2908 3089 3298 154 Size: 2754 181 209 1766 Fragments arranged by size: 2754 1766 209 181 BsrGI T'GTAC_A Cuts at: 434 434 Size: 4910 BssSI C'ACGA_G Cuts at: 3181 3539 3181 Size: 358 4552 BstAPI GCAn_nnn'nTGC Cuts at: 1105 1419 2287 1105 Size: 314 868 3728 Fragments arranged by size: 3728 868 314 BstBI TT'CG_AA Cuts at: 563 3271 563 Size: 2708 2202 BstXI CCAn_nnnn'nTGG Cuts at: 1041 1041 Size: 4910 BstYI r'GATC_y Cuts at: 1 494 1044 1217 1935 2402 2425 2760 3006 Size: 493 550 173 718 467 23 335 246 Cuts at: 3006 4018 4104 4116 4462 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 718 550 493 467 449 346 335 246 173 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1777 1777 Size: 4910 BtgI C'CryG_G Cuts at: 107 467 501 1109 1469 107 Size: 360 34 608 360 3548 Fragments arranged by size: 3548 608 360 360 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 778 790 2860 3041 3204 778 Size: 12 2070 181 163 2484 Fragments arranged by size: 2484 2070 181 163 12 BtsI GCAGTG_nn' Cuts at: 1009 1305 2153 1009 Size: 296 848 3766 Fragments arranged by size: 3766 848 296 DraI TTT'AAA Cuts at: 1909 1909 Size: 4910 DraIII CAC_nnn'GTG Cuts at: 414 432 414 Size: 18 4892 DrdI GACnn_nn'nnGTC Cuts at: 2616 3474 2616 Size: 858 4052 EaeI y'GGCC_r Cuts at: 360 972 2495 2669 3060 3087 4432 360 Size: 612 1523 174 391 27 1345 838 Fragments arranged by size: 1523 1345 838 612 391 174 27 EagI C'GGCC_G Cuts at: 2495 2495 Size: 4910 EarI CTCTTCn'nnn_ Cuts at: 442 595 821 1247 1287 1343 2933 3143 442 Size: 153 226 426 40 56 1590 210 2209 Fragments arranged by size: 2209 1590 426 226 210 153 56 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3118 3428 3574 4720 3118 Size: 310 146 1146 3308 Fragments arranged by size: 3308 1146 310 146 EcoICRI GAG'CTC Cuts at: 1578 1922 1578 Size: 344 4566 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 110 466 769 802 1170 1594 1684 2735 3167 Size: 356 303 33 368 424 90 1051 432 Cuts at: 3167 3914 4182 110 Size: 747 268 838 Fragments arranged by size: 1051 838 747 432 424 368 356 303 268 90 33 EcoNI CCTnn'n_nnAGG Cuts at: 1666 1666 Size: 4910 EcoO109I rG'GnC_Cy Cuts at: 650 2049 650 Size: 1399 3511 EcoRI G'AATT_C Cuts at: 1799 1914 1799 Size: 115 4795 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1401 2929 1401 Size: 1528 3382 FspI TGC'GCA Cuts at: 2691 2691 Size: 4910 HaeII r_GCGC'y Cuts at: 155 1662 1725 2344 2592 3614 4593 155 Size: 1507 63 619 248 1022 979 472 Fragments arranged by size: 1507 1022 979 619 472 248 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 903 935 1050 1053 1082 1085 1116 1148 1161 Size: 32 115 3 29 3 31 32 13 Cuts at: 1161 1193 1314 1346 1447 1479 1562 1594 2752 Size: 32 121 32 101 32 83 32 1158 Cuts at: 2752 2782 2784 2814 903 Size: 30 2 30 2999 Fragments arranged by size: 2999 1158 121 115 101 83 32 32 32 32 32 32 31 30 30 29 13 3 3 2 HincII GTy'rAC Cuts at: 321 630 1484 1949 321 Size: 309 854 465 3282 Fragments arranged by size: 3282 854 465 309 HindIII A'AGCT_T Cuts at: 796 796 Size: 4910 HpaI GTT'AAC Cuts at: 630 630 Size: 4910 KasI G'GCGC_C Cuts at: 151 2340 2588 151 Size: 2189 248 2473 Fragments arranged by size: 2473 2189 248 KpnI G_GTAC'C Cuts at: 1199 1357 1930 1199 Size: 158 573 4179 Fragments arranged by size: 4179 573 158 MluI A'CGCG_T Cuts at: 4343 4343 Size: 4910 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 766 1021 3581 3765 766 Size: 255 2560 184 1911 Fragments arranged by size: 2560 1911 255 184 MscI TGG'CCA Cuts at: 974 2671 974 Size: 1697 3213 MslI CAynn'nnrTG Cuts at: 1114 1841 2899 3026 1114 Size: 727 1058 127 2998 Fragments arranged by size: 2998 1058 727 127 MspA1I CmG'CkG Cuts at: 149 503 536 757 1401 1426 2021 2335 2695 Size: 354 33 221 644 25 595 314 360 Cuts at: 2695 3708 3953 149 Size: 1013 245 1106 Fragments arranged by size: 1106 1013 644 595 360 354 314 245 221 33 25 NaeI GCC'GGC Cuts at: 156 3091 156 Size: 2935 1975 NarI GG'CG_CC Cuts at: 152 2341 2589 152 Size: 2189 248 2473 Fragments arranged by size: 2473 2189 248 NcoI C'CATG_G Cuts at: 107 1109 107 Size: 1002 3908 NgoMIV G'CCGG_C Cuts at: 154 3089 154 Size: 2935 1975 NspI r_CATG'y Cuts at: 327 1963 2463 3370 327 Size: 1636 500 907 1867 Fragments arranged by size: 1867 1636 907 500 PciI A'CATG_T Cuts at: 3366 3366 Size: 4910 PflMI CCAn_nnn'nTGG Cuts at: 985 985 Size: 4910 PfoI T'CCnGG_A Cuts at: 1532 1532 Size: 4910 PmeI GTTT'AAAC Cuts at: 1909 1909 Size: 4910 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 4049 Size: 32 4878 PsiI TTA'TAA Cuts at: 1207 1207 Size: 4910 PstI C_TGCA'G Cuts at: 762 1016 1164 1957 2642 762 Size: 254 148 793 685 3030 Fragments arranged by size: 3030 793 685 254 148 PvuI CG_AT'CG Cuts at: 104 104 Size: 4910 PvuII CAG'CTG Cuts at: 757 1401 2335 2695 757 Size: 644 934 360 2972 Fragments arranged by size: 2972 934 644 360 RsrII CG'GwC_CG Cuts at: 3105 3105 Size: 4910 SacI G_AGCT'C Cuts at: 1580 1924 1580 Size: 344 4566 SacII CC_GC'GG Cuts at: 504 504 Size: 4910 SalI G'TCGA_C Cuts at: 319 1947 319 Size: 1628 3282 SapI GCTCTTCn'nnn_ Cuts at: 2933 3143 2933 Size: 210 4700 SbfI CC_TGCA'GG Cuts at: 1957 1957 Size: 4910 ScaI AGT'ACT Cuts at: 4427 4427 Size: 4910 SexAI A'CCwGG_T Cuts at: 410 410 Size: 4910 SfcI C'TryA_G Cuts at: 33 758 1012 1160 1581 1953 2638 3631 3822 Size: 725 254 148 421 372 685 993 191 Cuts at: 3822 33 Size: 1121 Fragments arranged by size: 1121 993 725 685 421 372 254 191 148 SfoI GGC'GCC Cuts at: 153 2342 2590 153 Size: 2189 248 2473 Fragments arranged by size: 2473 2189 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4910 SgrAI Cr'CCGG_yG Cuts at: 154 154 Size: 4910 SmaI CCC'GGG Cuts at: 164 525 1932 164 Size: 361 1407 3142 Fragments arranged by size: 3142 1407 361 SmlI C'TyrA_G Cuts at: 403 1747 2065 2980 3472 3734 403 Size: 1344 318 915 492 262 1579 Fragments arranged by size: 1579 1344 915 492 318 262 SpeI A'CTAG_T Cuts at: 67 1762 67 Size: 1695 3215 SphI G_CATG'C Cuts at: 1963 2463 1963 Size: 500 4410 StuI AGG'CCT Cuts at: 716 716 Size: 4910 StyI C'CwwG_G Cuts at: 107 1109 1385 1703 2044 2398 107 Size: 1002 276 318 341 354 2619 Fragments arranged by size: 2619 1002 354 341 318 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 859 2493 2626 3085 3294 859 Size: 1634 133 459 209 2475 Fragments arranged by size: 2475 1634 459 209 133 TatI w'GTAC_w Cuts at: 191 434 475 4425 191 Size: 243 41 3950 676 Fragments arranged by size: 3950 676 243 41 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4427 4459 4427 Size: 32 4878 Tth111I GACn'n_nGTC Cuts at: 2707 2707 Size: 4910 XbaI T'CTAG_A Cuts at: 1248 1941 1248 Size: 693 4217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 982 2405 982 Size: 1423 3487 XmaI C'CCGG_G Cuts at: 162 523 1930 162 Size: 361 1407 3142 Fragments arranged by size: 3142 1407 361 XmnI GAAnn'nnTTC Cuts at: 559 559 Size: 4910 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AhdI AleI AloI ApaI AscI AvrII BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SanDI SfiI SnaBI SrfI SspI SwaI XhoI ZraI