(Linear) (Six-base) MAPSORT of: KIBB7820.seq Check: 5424 from: 1 to: 1797 KIBB7820.orf With 182 enzymes: SgfI * October 7, 2009 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 926 1797 Size: 926 871 AccI GT'mk_AC Cuts at: 0 212 1797 Size: 212 1585 Acc65I G'GTAC_C Cuts at: 0 1087 1245 1797 Size: 1087 158 552 Fragments arranged by size: 1087 552 158 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 661 694 1576 1797 Size: 358 303 33 882 221 Fragments arranged by size: 882 358 303 221 33 AflII C'TTAA_G Cuts at: 0 1639 1797 Size: 1639 158 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 593 1797 Size: 593 1204 AlwNI CAG_nnn'CTG Cuts at: 0 261 748 1392 1797 Size: 261 487 644 405 Fragments arranged by size: 644 487 405 261 ApoI r'AATT_y Cuts at: 0 1256 1600 1691 1797 Size: 1256 344 91 106 Fragments arranged by size: 1256 344 106 91 AvaI C'yCGr_G Cuts at: 0 54 415 1797 Size: 54 361 1382 Fragments arranged by size: 1382 361 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1079 1112 1797 Size: 1079 33 685 Fragments arranged by size: 1079 685 33 BamHI G'GATC_C Cuts at: 0 386 1109 1797 Size: 386 723 688 Fragments arranged by size: 723 688 386 BanI G'GyrC_C Cuts at: 0 43 1087 1245 1797 Size: 43 1044 158 552 Fragments arranged by size: 1044 552 158 43 BanII G_rGCy'C Cuts at: 0 104 1472 1797 Size: 104 1368 325 Fragments arranged by size: 1368 325 104 BbeI G_GCGC'C Cuts at: 0 47 1797 Size: 47 1750 BbvCI CC'TCA_GC Cuts at: 0 662 1797 Size: 662 1135 BclI T'GATC_A Cuts at: 0 125 1797 Size: 125 1672 BglI GCCn_nnn'nGGC Cuts at: 0 872 1045 1797 Size: 872 173 752 Fragments arranged by size: 872 752 173 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1142 1797 Size: 1142 655 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 1062 1486 1797 Size: 2 1060 424 311 Fragments arranged by size: 1060 424 311 2 Bpu10I CC'TnA_GC Cuts at: 0 548 662 1797 Size: 548 114 1135 Fragments arranged by size: 1135 548 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 1797 Size: 280 1517 BsaHI Gr'CG_yC Cuts at: 0 44 50 1797 Size: 44 6 1747 Fragments arranged by size: 1747 44 6 BsaWI w'CCGG_w Cuts at: 0 383 1797 Size: 383 1414 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1357 1387 1797 Size: 1357 30 410 Fragments arranged by size: 1357 410 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1715 1797 Size: 1715 82 BseYI C'CCAG_C Cuts at: 0 399 1797 Size: 399 1398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 806 1797 Size: 806 991 BsiHKAI G_wGCw'C Cuts at: 0 888 1472 1797 Size: 888 584 325 Fragments arranged by size: 888 584 325 Bsp1286I G_dGCh'C Cuts at: 0 104 888 1472 1797 Size: 104 784 584 325 Fragments arranged by size: 784 584 325 104 BspEI T'CCGG_A Cuts at: 0 383 1797 Size: 383 1414 BspHI T'CATG_A Cuts at: 0 1728 1797 Size: 1728 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 369 926 1797 Size: 369 557 871 Fragments arranged by size: 871 557 369 BsrBI CCG'CTC Cuts at: 0 1107 1797 Size: 1107 690 BsrDI GCAATG_nn' Cuts at: 0 1013 1309 1797 Size: 1013 296 488 Fragments arranged by size: 1013 488 296 BsrFI r'CCGG_y Cuts at: 0 46 1797 Size: 46 1751 BsrGI T'GTAC_A Cuts at: 0 326 1797 Size: 326 1471 BstAPI GCAn_nnn'nTGC Cuts at: 0 997 1311 1797 Size: 997 314 486 Fragments arranged by size: 997 486 314 BstBI TT'CG_AA Cuts at: 0 455 1797 Size: 455 1342 BstXI CCAn_nnnn'nTGG Cuts at: 0 933 1797 Size: 933 864 BstYI r'GATC_y Cuts at: 0 386 936 1109 1797 Size: 386 550 173 688 Fragments arranged by size: 688 550 386 173 Bsu36I CC'TnA_GG Cuts at: 0 1669 1797 Size: 1669 128 BtgI C'CryG_G Cuts at: 0 359 393 1001 1361 1797 Size: 359 34 608 360 436 Fragments arranged by size: 608 436 360 359 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 670 682 1797 Size: 670 12 1115 Fragments arranged by size: 1115 670 12 BtsI GCAGTG_nn' Cuts at: 0 901 1197 1797 Size: 901 296 600 Fragments arranged by size: 901 600 296 DraIII CAC_nnn'GTG Cuts at: 0 306 324 1797 Size: 306 18 1473 Fragments arranged by size: 1473 306 18 EaeI y'GGCC_r Cuts at: 0 252 864 1797 Size: 252 612 933 Fragments arranged by size: 933 612 252 EarI CTCTTCn'nnn_ Cuts at: 0 334 487 713 1139 1179 1235 1797 Size: 334 153 226 426 40 56 562 Fragments arranged by size: 562 426 334 226 153 56 40 EcoICRI GAG'CTC Cuts at: 0 1470 1797 Size: 1470 327 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 358 661 694 1062 1486 1576 1797 Size: 2 356 303 33 368 424 90 221 Fragments arranged by size: 424 368 356 303 221 90 33 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 1558 1797 Size: 1558 239 EcoO109I rG'GnC_Cy Cuts at: 0 542 1797 Size: 542 1255 EcoRI G'AATT_C Cuts at: 0 1691 1797 Size: 1691 106 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1293 1797 Size: 1293 504 HaeII r_GCGC'y Cuts at: 0 47 1554 1617 1797 Size: 47 1507 63 180 Fragments arranged by size: 1507 180 63 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 795 827 942 945 974 977 1008 1040 Size: 795 32 115 3 29 3 31 32 Cuts at: 1040 1053 1085 1206 1238 1339 1371 1454 1486 Size: 13 32 121 32 101 32 83 32 Cuts at: 1486 1797 Size: 311 Fragments arranged by size: 795 311 121 115 101 83 32 32 32 32 32 32 31 29 13 3 3 HincII GTy'rAC Cuts at: 0 213 522 1376 1797 Size: 213 309 854 421 Fragments arranged by size: 854 421 309 213 HindIII A'AGCT_T Cuts at: 0 688 1797 Size: 688 1109 HpaI GTT'AAC Cuts at: 0 522 1797 Size: 522 1275 KasI G'GCGC_C Cuts at: 0 43 1797 Size: 43 1754 KpnI G_GTAC'C Cuts at: 0 1091 1249 1797 Size: 1091 158 548 Fragments arranged by size: 1091 548 158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 658 913 1797 Size: 658 255 884 Fragments arranged by size: 884 658 255 MscI TGG'CCA Cuts at: 0 866 1797 Size: 866 931 MslI CAynn'nnrTG Cuts at: 0 1006 1733 1797 Size: 1006 727 64 Fragments arranged by size: 1006 727 64 MspA1I CmG'CkG Cuts at: 0 41 395 428 649 1293 1318 1797 Size: 41 354 33 221 644 25 479 Fragments arranged by size: 644 479 354 221 41 33 25 NaeI GCC'GGC Cuts at: 0 48 1797 Size: 48 1749 NarI GG'CG_CC Cuts at: 0 44 1797 Size: 44 1753 NcoI C'CATG_G Cuts at: 0 1001 1797 Size: 1001 796 NgoMIV G'CCGG_C Cuts at: 0 46 1797 Size: 46 1751 NspI r_CATG'y Cuts at: 0 219 1797 Size: 219 1578 PflMI CCAn_nnn'nTGG Cuts at: 0 877 1797 Size: 877 920 PfoI T'CCnGG_A Cuts at: 0 1424 1797 Size: 1424 373 PsiI TTA'TAA Cuts at: 0 1099 1797 Size: 1099 698 PstI C_TGCA'G Cuts at: 0 654 908 1056 1797 Size: 654 254 148 741 Fragments arranged by size: 741 654 254 148 PvuII CAG'CTG Cuts at: 0 649 1293 1797 Size: 649 644 504 Fragments arranged by size: 649 644 504 SacI G_AGCT'C Cuts at: 0 1472 1797 Size: 1472 325 SacII CC_GC'GG Cuts at: 0 396 1797 Size: 396 1401 SalI G'TCGA_C Cuts at: 0 211 1797 Size: 211 1586 SexAI A'CCwGG_T Cuts at: 0 302 1797 Size: 302 1495 SfcI C'TryA_G Cuts at: 0 650 904 1052 1473 1797 Size: 650 254 148 421 324 Fragments arranged by size: 650 421 324 254 148 SfoI GGC'GCC Cuts at: 0 45 1797 Size: 45 1752 SgrAI Cr'CCGG_yG Cuts at: 0 46 1797 Size: 46 1751 SmaI CCC'GGG Cuts at: 0 56 417 1797 Size: 56 361 1380 Fragments arranged by size: 1380 361 56 SmlI C'TyrA_G Cuts at: 0 295 1639 1797 Size: 295 1344 158 Fragments arranged by size: 1344 295 158 SpeI A'CTAG_T Cuts at: 0 1654 1797 Size: 1654 143 StuI AGG'CCT Cuts at: 0 608 1797 Size: 608 1189 StyI C'CwwG_G Cuts at: 0 1001 1277 1595 1797 Size: 1001 276 318 202 Fragments arranged by size: 1001 318 276 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 751 1797 Size: 751 1046 TatI w'GTAC_w Cuts at: 0 83 326 367 1797 Size: 83 243 41 1430 Fragments arranged by size: 1430 243 83 41 XbaI T'CTAG_A Cuts at: 0 1140 1797 Size: 1140 657 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 874 1797 Size: 874 923 XmaI C'CCGG_G Cuts at: 0 54 415 1797 Size: 54 361 1382 Fragments arranged by size: 1382 361 54 XmnI GAAnn'nnTTC Cuts at: 0 451 1797 Size: 451 1346 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AlfI AlwNI ApoI AvaI BaeI BamHI BanI BanII BbeI BbvCI BclI BglI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PfoI PsiI PstI PvuII SacI SacII SalI SexAI SfcI SfoI SgrAI SmaI SmlI SpeI StuI StyI TaqII TatI XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII BbsI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BsaI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BssHII BssSI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoRV FseI FspI FspAI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SanDI SapI SbfI ScaI SfiI SgfI SnaBI SphI SrfI SspI SwaI TstI Tth111I XhoI ZraI