Restriction map for
pF1KA0671
(Circular) (Six-base) MAPSORT of: pF1KA0671.seq Check: 9222 from: 1 to: 4721
pF1KA0671 4721 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1577 1577
Size: 4721
AccI GT'mk_AC
Cuts at: 1759 1759
Size: 4721
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1325 2546 2978 3725 3993 1325
Size: 1221 432 747 268 2053
Fragments arranged by size:
2053 1221 747 432 268
AflII C'TTAA_G
Cuts at: 282 282
Size: 4721
AflIII A'CryG_T
Cuts at: 2073 3177 4154 2073
Size: 1104 977 2640
Fragments arranged by size:
2640 1104 977
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1333 1333
Size: 4721
AlwNI CAG_nnn'CTG
Cuts at: 823 2026 3593 823
Size: 1203 1567 1951
Fragments arranged by size:
1951 1567 1203
ApaLI G'TGCA_C
Cuts at: 1590 3491 1590
Size: 1901 2820
ApoI r'AATT_y
Cuts at: 327 1725 4074 4330 327
Size: 1398 2349 256 718
Fragments arranged by size:
2349 1398 718 256
AseI AT'TA_AT
Cuts at: 20 745 20
Size: 725 3996
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 1741 1741
Size: 4721
BamHI G'GATC_C
Cuts at: 1746 1746
Size: 4721
BanI G'GyrC_C
Cuts at: 1737 2151 2399 2434 1737
Size: 414 248 35 4024
Fragments arranged by size:
4024 414 248 35
BanII G_rGCy'C
Cuts at: 832 1619 1735 2765 832
Size: 787 116 1030 2788
Fragments arranged by size:
2788 1030 787 116
BbeI G_GCGC'C
Cuts at: 2155 2403 2155
Size: 248 4473
BbvCI CC'TCA_GC
Cuts at: 263 263
Size: 4721
BciVI GTATCCnnnnn_n'
Cuts at: 648 1080 2619 3380 4721 648
Size: 432 1539 761 1341 648
Fragments arranged by size:
1539 1341 761 648 432
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 1594 2346 2439 3495 1594
Size: 752 93 1056 2820
Fragments arranged by size:
2820 1056 752 93
BmrI ACTGGGnnnn_n'
Cuts at: 316 1012 1263 1487 1933 2014 2349 4493 316
Size: 696 251 224 446 81 335 2144 544
Fragments arranged by size:
2144 696 544 446 335 251 224 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 1160
Size: 4721
Bpu10I CC'TnA_GC
Cuts at: 263 1321 263
Size: 1058 3663
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1897 2776 3268 3566 772
Size: 1125 879 492 298 1927
Fragments arranged by size:
1927 1125 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4425 4425
Size: 4721
BsaAI yAC'GTr
Cuts at: 1598 2074 1598
Size: 476 4245
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 1427 2152 2400 3102 4598 1427
Size: 725 248 702 1496 1550
Fragments arranged by size:
1550 1496 725 702 248
BsaWI w'CCGG_w
Cuts at: 1910 2431 3109 3383 3530 3941 1910
Size: 521 678 274 147 411 2690
Fragments arranged by size:
2690 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 190
Size: 30 4691
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 251 1443 251
Size: 1192 3529
BseYI C'CCAG_C
Cuts at: 2146 2904 3481 3977 2146
Size: 758 577 496 2890
Fragments arranged by size:
2890 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 962
Size: 4721
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3517 104
Size: 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1594 1735 2513 2703 3495 1594
Size: 141 778 190 792 2820
Fragments arranged by size:
2820 792 778 190 141
Bsp1286I G_dGCh'C
Cuts at: 832 1594 1619 1735 2346 2439 2513 2703 2765
Size: 762 25 116 611 93 74 190 62
Cuts at: 2765 3495 832
Size: 730 2058
Fragments arranged by size:
2058 762 730 611 190 116 93 74
62 25
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 168 3897 168
Size: 3729 992
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1577 1771 1985 2287 2668 1577
Size: 194 214 302 381 3630
Fragments arranged by size:
3630 381 302 214 194
BsrBI CCG'CTC
Cuts at: 50 3013 3067 4551 4716 50
Size: 2963 54 1484 165 55
Fragments arranged by size:
2963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1516 2633 1516
Size: 1117 3604
BsrFI r'CCGG_y
Cuts at: 2719 2900 3109 2719
Size: 181 209 4331
Fragments arranged by size:
4331 209 181
BsrGI T'GTAC_A
Cuts at: 588 588
Size: 4721
BssSI C'ACGA_G
Cuts at: 1213 2992 3350 1213
Size: 1779 358 2584
Fragments arranged by size:
2584 1779 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2098 2098
Size: 4721
BstBI TT'CG_AA
Cuts at: 322 3082 322
Size: 2760 1961
BstYI r'GATC_y
Cuts at: 1 1378 1488 1746 2213 2236 2571 2817 3829
Size: 1377 110 258 467 23 335 246 1012
Cuts at: 3829 3915 3927 4273 1
Size: 86 12 346 449
Fragments arranged by size:
1377 1012 467 449 346 335 258 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 463 107
Size: 356 4365
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 1206 1408 1964 1206
Size: 202 556 3963
Fragments arranged by size:
3963 556 202
DraI TTT'AAA
Cuts at: 1649 1720 1649
Size: 71 4650
DraIII CAC_nnn'GTG
Cuts at: 1100 1100
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3285 2427
Size: 858 3863
EaeI y'GGCC_r
Cuts at: 2306 2480 2871 2898 4243 2306
Size: 174 391 27 1345 2784
Fragments arranged by size:
2784 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 166 432 1076 1354 2744 2954 166
Size: 266 644 278 1390 210 1933
Fragments arranged by size:
1933 1390 644 278 266 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1354 2929 3239 3385 4531 1354
Size: 1575 310 146 1146 1544
Fragments arranged by size:
1575 1544 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1733 1733
Size: 4721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 1325 2546 2978 3725 3993 1160
Size: 165 1221 432 747 268 1888
Fragments arranged by size:
1888 1221 747 432 268 165
EcoO109I rG'GnC_Cy
Cuts at: 1860 1860
Size: 4721
EcoRI G'AATT_C
Cuts at: 327 1725 327
Size: 1398 3323
EcoRV GAT'ATC
Cuts at: 1136 1136
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 726 913 2740 726
Size: 187 1827 2707
Fragments arranged by size:
2707 1827 187
FspI TGC'GCA
Cuts at: 1334 2502 1334
Size: 1168 3553
HaeII r_GCGC'y
Cuts at: 573 2155 2403 3425 4404 573
Size: 1582 248 1022 979 890
Fragments arranged by size:
1582 1022 979 890 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 433 465 1688 1720 2563 2593 2595
Size: 32 211 32 1223 32 843 30 2
Cuts at: 2595 2625 190
Size: 30 2286
Fragments arranged by size:
2286 1223 843 211 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
KasI G'GCGC_C
Cuts at: 2151 2399 2151
Size: 248 4473
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 233 1670 3392 3576 233
Size: 1437 1722 184 1378
Fragments arranged by size:
1722 1437 1378 184
MscI TGG'CCA
Cuts at: 2482 2482
Size: 4721
MslI CAynn'nnrTG
Cuts at: 1154 2710 2837 1154
Size: 1556 127 3038
Fragments arranged by size:
3038 1556 127
MspA1I CmG'CkG
Cuts at: 1832 2146 2506 3519 3764 1832
Size: 314 360 1013 245 2789
Fragments arranged by size:
2789 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 2152 2400 2152
Size: 248 4473
NcoI C'CATG_G
Cuts at: 107 463 107
Size: 356 4365
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NspI r_CATG'y
Cuts at: 460 1390 1506 1774 2274 3181 460
Size: 930 116 268 500 907 2000
Fragments arranged by size:
2000 930 907 500 268 116
PciI A'CATG_T
Cuts at: 3177 3177
Size: 4721
PflMI CCAn_nnn'nTGG
Cuts at: 469 469
Size: 4721
PfoI T'CCnGG_A
Cuts at: 1020 1020
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PsiI TTA'TAA
Cuts at: 1484 1664 1484
Size: 180 4541
PstI C_TGCA'G
Cuts at: 1562 1588 1768 2453 1562
Size: 26 180 685 3830
Fragments arranged by size:
3830 685 180 26
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 2146 2506 2146
Size: 360 4361
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 1735 1735
Size: 4721
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SfcI C'TryA_G
Cuts at: 33 620 875 1558 1584 1764 2449 3442 3633
Size: 587 255 683 26 180 685 993 191
Cuts at: 3633 33
Size: 1121
Fragments arranged by size:
1121 993 685 683 587 255 191 180
26
SfoI GGC'GCC
Cuts at: 2153 2401 2153
Size: 248 4473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1743 1743
Size: 4721
SmlI C'TyrA_G
Cuts at: 282 751 1876 2791 3283 3545 282
Size: 469 1125 915 492 262 1458
Fragments arranged by size:
1458 1125 915 492 469 262
SnaBI TAC'GTA
Cuts at: 1598 1598
Size: 4721
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1390 1774 2274 1390
Size: 384 500 3837
Fragments arranged by size:
3837 500 384
SspI AAT'ATT
Cuts at: 294 294
Size: 4721
StyI C'CwwG_G
Cuts at: 107 463 1855 2209 107
Size: 356 1392 354 2619
Fragments arranged by size:
2619 1392 356 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 2304 2437 2896 3105 154
Size: 2150 133 459 209 1770
Fragments arranged by size:
2150 1770 459 209 133
TatI w'GTAC_w
Cuts at: 588 4236 588
Size: 3648 1073
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4238 4270 4238
Size: 32 4689
Tth111I GACn'n_nGTC
Cuts at: 2518 2518
Size: 4721
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2216 114
Size: 2102 2619
XmaI C'CCGG_G
Cuts at: 1741 1741
Size: 4721
XmnI GAAnn'nnTTC
Cuts at: 136 160 441 1286 136
Size: 24 281 845 3571
Fragments arranged by size:
3571 845 281 24
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI