Restriction map for pF1KA0335
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AspEI       
AviII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspMI       BsrBI       BsrFI       BssHII      
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       FauI        
FbaI        FseI        FspI        HaeII       HapII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KspI        MamI        MflI        
MluI        MluNI       MroI        MscI        MspI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    StuI        SwaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I       1774  GGTACC               G^GTACC
AlwI         1903  GGATC                GGATC(4/5)
Asp718I      1774  GGTACC               G^GTACC
AspI         2667  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        2067  GAGCTC               G[GA]GC[CT]^C
BpmI         2308  CTCCAG               CTGGAG(16/14)
BsmFI        1218  GGGAC                GGGAC(10/14)
BspHI        1241  TCATGA               T^CATGA
BstBI        2487  TTCGAA               TT^CGAA
BstXI         648  CCAGGATGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII        1904  GATC                 ^GATC
DraIII        599  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          429  CCATGG               C^C[GA][CT]GG
EcoRV        1599  GATATC               GAT^ATC
HaeIII       3145  GGCC                 GG^CC
HgaI         4060  GACGC                GACGC(5/10)
KpnI         1774  GGTACC               GGTAC^C
MboI         1904  GATC                 ^GATC
MfeI         2353  CAATTG               C^AATTG
MunI         2353  CAATTG               C^AATTG
NcoI          429  CCATGG               C^CATGG
NdeII        1904  GATC                 ^GATC
NspV         2487  TTCGAA               TT^CGAA
PflFI        2667  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1260  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          923  CTGCAG               CTGCA^G
PvuII         357  CAGCTG               CAG^CTG
RcaI         1241  TCATGA               T^CATGA
SacI         2067  GAGCTC               GAGCT^C
Sau3AI       1904  GATC                 ^GATC
ScaI         3376  AGTACT               AGT^ACT
SfcI          923  CTGCAG               C^T[GA][CT]AG
SfuI         2487  TTCGAA               TT^CGAA
SpeI         2052  ACTAGT               A^CTAGT
Tth111I      2667  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI         2972  CCGC                 CCGC(-3/-1)
             3981  GCGG                
AseI         1858  ATTAAT               AT^TAAT
             2542  ATTAAT              
AsnI         1858  ATTAAT               AT^TAAT
             2542  ATTAAT              
Asp700I       972  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2490  GAAAGATTTC          
AspHI        2067  GAGCTC               G[AT]GC[AT]^C
             3216  GAGCAC              
AvaI         1762  CCCGAG               C^[CT]CG[GA]G
             4427  CTCGGG              
AvaII        1219  GGACC                G^G[AT]CC
             1261  GGACC               
AvrII        3949  CCTAGG               C^CTAGG
             4021  CCTAGG              
BciVI        4383  GGATAC               GTATCC(6/5)
             2894  GTATCC              
BlnI         3949  CCTAGG               C^CTAGG
             4021  CCTAGG              
BsiHKAI      2067  GAGCTC               G[AT]GC[AT]^C
             3216  GAGCAC              
BsmAI         374  GAGAC                GTCTC(1/5)
             4407  GAGAC               
BsoBI        1762  CCCGAG               C^[CT]CG[GA]G
             4427  CTCGGG              
Bsp1407I      286  TGTACA               T^GTACA
             2198  TGTACA              
BsrGI         286  TGTACA               T^GTACA
             2198  TGTACA              
BssSI        2458  CACGAG               CACGAG(-5/-1)
             2999  CTCGTG              
Cac8I        2423  GCTTGC               GC[ACGT]^[ACGT]GC
             3603  GCCAGC              
DraII        1260  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3144  AGGCCCT             
EarI          319  GAAGAG               CTCTTC(1/4)
             1213  GAAGAG              
EcoO109I     1260  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3144  AGGCCCT             
EcoT22I      1823  ATGCAT               ATGCA^T
             1843  ATGCAT              
Ksp632I       319  GAAGAG               CTCTTC(1/4)
             1213  GAAGAG              
MspA1I        357  CAGCTG               C[AC]G^C[GT]G
             2972  CCGCTG              
NsiI         1823  ATGCAT               ATGCA^T
             1843  ATGCAT              
PflMI        1458  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2995  CCAGCTCGTGG         
PshBI        1858  ATTAAT               AT^TAAT
             2542  ATTAAT              
SspBI         286  TGTACA               T^GTACA
             2198  TGTACA              
Van91I       1458  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2995  CCAGCTCGTGG         
XbaI         3565  TCTAGA               T^CTAGA
             3814  TCTAGA              
XmnI          972  GAAGACGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2490  GAAAGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AcsI        AfaI        AflII       AflIII      AluI        
AlwNI       ApoI        BanI        BbsI        BbvI        
BfaI        BfrI        BmyI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        Bsp1286I    BspLU11I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        DraI        EcoRII      EcoT14I     
Fnu4HI      FokI        HincII      HindII      HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboII       MnlI        MseI        MslI        MvaI        
MwoI        NlaIII      NlaIV       NspI        PleI        
RsaI        Sau96I      ScrFI       SfaNI       SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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