Restriction map for pF1KA0288
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AocI        
Aor51HI     AscI        AseI        AsnI        AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsiWI       BsmI        BspDI       
BspHI       BsrDI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      Eco47III    Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HpaI        KasI        MfeI        MluI        
MseI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    RcaI        RsrII       SfiI        SfoI        
SfuI        SnaBI       SpeI        SphI        SspI        
SwaI        Tru9I       XbaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       2861  GGTACC               G^GTACC
AccI          663  GTCGAC               GT^[AC][GT]AC
AccIII        892  TCCGGA               T^CCGGA
AcsI          529  GAATTT               [GA]^AATT[CT]
AflIII       2576  ACATGT               A^C[GA][CT]GT
AhdI         1339  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         2647  GGGCCC               GGGCC^C
ApoI          529  GAATTT               [GA]^AATT[CT]
Asp700I      2604  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2861  GGTACC               G^GTACC
AspEI        1339  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1392  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI         2344  GTCTTC               GAAGAC(2/6)
BbvCI        1812  GCTGAGG              CCTCAGC(-5/-2)
BfaI         2215  CTAG                 C^TAG
BpuAI        2344  GTCTTC               GAAGAC(2/6)
BseAI         892  TCCGGA               T^CCGGA
Bsp1407I     2573  TGTACA               T^GTACA
BspEI         892  TCCGGA               T^CCGGA
BspLU11I     2576  ACATGT               A^CATGT
BsrGI        2573  TGTACA               T^GTACA
BstEII       3165  GGTCACC              G^GT[ACGT]ACC
BstPI        3165  GGTCACC              G^GT[ACGT]ACC
DrdI         2560  GACCCCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1339  GACCGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3165  GGTCACC              G^GT[ACGT]ACC
HindIII      2467  AAGCTT               A^AGCTT
KpnI         2861  GGTACC               GGTAC^C
KspI         2103  CCGCGG               CCGC^GG
MaeI         2215  CTAG                 C^TAG
MroI          892  TCCGGA               T^CCGGA
NdeI         2280  CATATG               CA^TATG
PaeR7I       2914  CTCGAG               C^TCGAG
PflFI        1392  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          42  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2536  GGGACCC              [GA]G^G[AT]CC[CT]
PvuI         2750  CGATCG               CGAT^CG
SacII        2103  CCGCGG               CCGC^GG
SalI          663  GTCGAC               G^TCGAC
SapI         1764  GCTCTTC              GCTCTTC(1/4)
SbfI         2064  CCTGCAGG             CCTGCA^GG
SexAI        1436  ACCTGGT              A^CC[AT]GGT
SgrAI        2682  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         1883  CCCGGG               CCC^GGG
Sse8387I     2064  CCTGCAGG             CCTGCA^GG
SspBI        2573  TGTACA               T^GTACA
Tth111I      1392  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         42  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1055  CCAACATCACGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2914  CTCGAG               C^TCGAG
XmaCI        1883  CCCGGG               C^CCGGG
XmaI         1883  CCCGGG               C^CCGGG
XmnI         2604  GAACTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BamHI        1802  GGATCC               G^GATCC
             2042  GGATCC              
BsaBI         261  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2499  GATCCTCATC          
BsaI           38  GAGACC               GGTCTC(1/5)
             3213  GAGACC              
BsaWI         563  ACCGGA               [AT]^CCGG[AT]
              892  TCCGGA              
BstXI          15  CCATCCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
               86  CCAGCACGGTGG        
DraII        2536  GGGACCC              [GA]G^G[ACGT]CC[CT]
             2646  AGGGCCC             
EagI         1016  CGGCCG               C^GGCCG
             2895  CGGCCG              
EclXI        1016  CGGCCG               C^GGCCG
             2895  CGGCCG              
Eco52I       1016  CGGCCG               C^GGCCG
             2895  CGGCCG              
EcoO109I     2536  GGGACCC              [GA]G^G[ACGT]CC[CT]
             2646  AGGGCCC             
HincII        663  GTCGAC               GT[CT]^[GA]AC
             2668  GTCAAC              
HindII        663  GTCGAC               GT[CT]^[GA]AC
             2668  GTCAAC              
MaeII        2519  ACGT                 A^CGT
             2666  ACGT                
MamI          261  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2499  GATCCTCATC          
PleI          865  GACTC                GAGTC(4/5)
             1124  GACTC               
ScaI         2717  AGTACT               AGT^ACT
             3077  AGTACT              
Tsp509I       530  AATT                 ^AATT
              752  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com