Restriction map for pF1KA0288
 (Linear) (Six-base) MAPSORT of: KIAA0288.seq  Check: 7937  from: 1  to: 3252



KIAA0288.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1659    2011    3252 
   Size:       1659     352    1241

  Fragments arranged by size: 

                1659    1241     352
 
AccI GT'mk_AC

Cuts at:      0     664    3252 
   Size:        664    2588
 
Acc65I G'GTAC_C

Cuts at:      0    2861    3252 
   Size:       2861     391
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1752    2008    2389    2715    3252 
   Size:       1752     256     381     326     537

  Fragments arranged by size: 

                1752     537     381     326     256
 
AflIII A'CryG_T

Cuts at:      0    2576    3252 
   Size:       2576     676
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1344    3252 
   Size:       1344    1908
 
AleI CACnn'nnGTG

Cuts at:      0     508    3252 
   Size:        508    2744
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     151     183    3252 
   Size:        151      32    3069

  Fragments arranged by size: 

                3069     151      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     378     426     453     736    1428    1437    1467 
   Size:        210     168      48      27     283     692       9      30

Cuts at:   1467    1503    1602    1761    2196    3252 
   Size:         36      99     159     435    1056

  Fragments arranged by size: 

                1056     692     435     283     210     168     159      99
                  48      36      30      27       9
 
ApaI G_GGCC'C

Cuts at:      0    2651    3252 
   Size:       2651     601
 
ApaLI G'TGCA_C

Cuts at:      0    1998    2140    2293    2521    3252 
   Size:       1998     142     153     228     731

  Fragments arranged by size: 

                1998     731     228     153     142
 
ApoI r'AATT_y

Cuts at:      0     529    3252 
   Size:        529    2723
 
AvaI C'yCGr_G

Cuts at:      0    1883    2026    2914    3252 
   Size:       1883     143     888     338

  Fragments arranged by size: 

                1883     888     338     143
 
BamHI G'GATC_C

Cuts at:      0    1802    2042    3252 
   Size:       1802     240    1210

  Fragments arranged by size: 

                1802    1210     240
 
BanI G'GyrC_C

Cuts at:      0     407    1168    2861    3252 
   Size:        407     761    1693     391

  Fragments arranged by size: 

                1693     761     407     391
 
BanII G_rGCy'C

Cuts at:      0       7     224    1586    1616    1685    1715    1937    2628 
   Size:          7     217    1362      30      69      30     222     691

Cuts at:   2628    2651    3230    3252 
   Size:         23     579      22

  Fragments arranged by size: 

                1362     691     579     222     217      69      30      30
                  23      22       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2337    3252 
   Size:       2337     915
 
BbvCI CC'TCA_GC

Cuts at:      0    1813    3252 
   Size:       1813    1439
 
BglI GCCn_nnn'nGGC

Cuts at:      0     133    1545    2084    2317    2891    3252 
   Size:        133    1412     539     233     574     361

  Fragments arranged by size: 

                1412     574     539     361     233     133
 
Bme1580I G_kGCm'C

Cuts at:      0     143    1099    2002    2144    2297    2525    2644    2651 
   Size:        143     956     903     142     153     228     119       7

Cuts at:   2651    3252 
   Size:        601

  Fragments arranged by size: 

                 956     903     601     228     153     143     142     119
                   7
 
BmgBI CAC'GTC

Cuts at:      0    2520    3252 
   Size:       2520     732
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1389    1393    1742    2523    3252 
   Size:       1389       4     349     781     729

  Fragments arranged by size: 

                1389     781     729     349       4
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     315    2313    3252 
   Size:        315    1998     939

  Fragments arranged by size: 

                1998     939     315
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     448    1126    1279    1489    1810    2143    3252 
   Size:        421      27     678     153     210     321     333    1109

  Fragments arranged by size: 

                1109     678     421     333     321     210     153      27
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1189    1813    3252 
   Size:       1189     624    1439

  Fragments arranged by size: 

                1439    1189     624
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     448    3252 
   Size:        217     231    2804

  Fragments arranged by size: 

                2804     231     217
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      32    3207    3252 
   Size:         32    3175      45

  Fragments arranged by size: 

                3175      45      32
 
BsaBI GATnn'nnATC

Cuts at:      0     265    2503    3252 
   Size:        265    2238     749

  Fragments arranged by size: 

                2238     749     265
 
BsaHI Gr'CG_yC

Cuts at:      0     701    1115    1828    2948    3252 
   Size:        701     414     713    1120     304

  Fragments arranged by size: 

                1120     713     701     414     304
 
BsaWI w'CCGG_w

Cuts at:      0     563     892    3252 
   Size:        563     329    2360

  Fragments arranged by size: 

                2360     563     329
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     901     931    1295    1325    2431    2461    2827    2857 
   Size:        901      30     364      30    1106      30     366      30

Cuts at:   2857    3252 
   Size:        395

  Fragments arranged by size: 

                1106     901     395     366     364      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1582    1591    1594    2140    2428    3238    3252 
   Size:       1582       9       3     546     288     810      14

  Fragments arranged by size: 

                1582     810     546     288      14       9       3
 
BseYI C'CCAG_C

Cuts at:      0      85     898     961    1065    2540    2563    2976    3252 
   Size:         85     813      63     104    1475      23     413     276

  Fragments arranged by size: 

                1475     813     413     276     104      85      63      23
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894    1355    1687    1948    3252 
   Size:        894     461     332     261    1304

  Fragments arranged by size: 

                1304     894     461     332     261
 
BsiEI CG_ry'CG

Cuts at:      0    1019    2753    2898    3252 
   Size:       1019    1734     145     354

  Fragments arranged by size: 

                1734    1019     354     145
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     224     332    1195    1305    1586    1595    2002 
   Size:          7     217     108     863     110     281       9     407

Cuts at:   2002    2033    2144    2297    2422    2525    3252 
   Size:         31     111     153     125     103     727

  Fragments arranged by size: 

                 863     727     407     281     217     153     125     111
                 110     108     103      31       9       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     970    1564    2065    2702    3154    3252 
   Size:        970     594     501     637     452      98

  Fragments arranged by size: 

                 970     637     594     501     452      98
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     143     224     332    1099    1195    1305    1586 
   Size:          7     136      81     108     767      96     110     281

Cuts at:   1586    1595    1616    1685    1715    1937    2002    2033    2144 
   Size:          9      21      69      30     222      65      31     111

Cuts at:   2144    2297    2422    2525    2628    2644    2651    3230    3252 
   Size:        153     125     103     103      16       7     579      22

  Fragments arranged by size: 

                 767     579     281     222     153     136     125     111
                 110     108     103     103      96      81      69      65
                  31      30      22      21      16       9       7       7
            
 
BspEI T'CCGG_A

Cuts at:      0     892    3252 
   Size:        892    2360
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     158    1659    2011    3252 
   Size:        158    1501     352    1241

  Fragments arranged by size: 

                1501    1241     352     158
 
BsrBI CCG'CTC

Cuts at:      0     656    1021    1209    3252 
   Size:        656     365     188    2043

  Fragments arranged by size: 

                2043     656     365     188
 
BsrFI r'CCGG_y

Cuts at:      0     189    1081    1269    1625    1660    1843    2186    2683 
   Size:        189     892     188     356      35     183     343     497

Cuts at:   2683    3252 
   Size:        569

  Fragments arranged by size: 

                 892     569     497     356     343     189     188     183
                  35
 
BsrGI T'GTAC_A

Cuts at:      0    2573    3252 
   Size:       2573     679
 
BssSI C'ACGA_G

Cuts at:      0     289     310     325    1004    1966    3252 
   Size:        289      21      15     679     962    1286

  Fragments arranged by size: 

                1286     962     679     289      21      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     210     378     453    1503    1761    3252 
   Size:        210     168      75    1050     258    1491

  Fragments arranged by size: 

                1491    1050     258     210     168      75
 
BstEII G'GTnAC_C

Cuts at:      0    3165    3252 
   Size:       3165      87
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      22      93    3252 
   Size:         22      71    3159

  Fragments arranged by size: 

                3159      71      22
 
BstYI r'GATC_y

Cuts at:      0     248     260    1802    2042    2498    3065    3252 
   Size:        248      12    1542     240     456     567     187

  Fragments arranged by size: 

                1542     567     456     248     240     187      12
 
BtgI C'CryG_G

Cuts at:      0     104     143     494    1717    1832    2103    2426    2450 
   Size:        104      39     351    1223     115     271     323      24

Cuts at:   2450    2456    2526    2702    2804    3047    3173    3188    3230 
   Size:          6      70     176     102     243     126      15      42

Cuts at:   3230    3252 
   Size:         22

  Fragments arranged by size: 

                1223     351     323     271     243     176     126     115
                 104     102      70      42      39      24      22      15
                   6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     252    1025    1709    3252 
   Size:        252     773     684    1543

  Fragments arranged by size: 

                1543     773     684     252
 
BtsI GCAGTG_nn'

Cuts at:      0     143     172     576     830    1303    3252 
   Size:        143      29     404     254     473    1949

  Fragments arranged by size: 

                1949     473     404     254     143      29
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2808    2843    3252 
   Size:       2808      35     409

  Fragments arranged by size: 

                2808     409      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2566    3252 
   Size:       2566     686
 
EaeI y'GGCC_r

Cuts at:      0      33     101     356     497     779    1016    1866    2354 
   Size:         33      68     255     141     282     237     850     488

Cuts at:   2354    2453    2895    3252 
   Size:         99     442     357

  Fragments arranged by size: 

                 850     488     442     357     282     255     237     141
                  99      68      33
 
EagI C'GGCC_G

Cuts at:      0    1016    2895    3252 
   Size:       1016    1879     357

  Fragments arranged by size: 

                1879    1016     357
 
EarI CTCTTCn'nnn_

Cuts at:      0    1241    1697    1771    3230    3252 
   Size:       1241     456      74    1459      22

  Fragments arranged by size: 

                1459    1241     456      74      22
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     988    1404    2380    2835    3252 
   Size:        988     416     976     455     417

  Fragments arranged by size: 

                 988     976     455     417     416
 
EcoICRI GAG'CTC

Cuts at:      0       5     222    1584    3252 
   Size:          5     217    1362    1668

  Fragments arranged by size: 

                1668    1362     217       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     448    1126    1279    1489    1752    1810    2008 
   Size:        421      27     678     153     210     263      58     198

Cuts at:   2008    2143    2389    2715    3252 
   Size:        135     246     326     537

  Fragments arranged by size: 

                 678     537     421     326     263     246     210     198
                 153     135      58      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2537    2647    3252 
   Size:       2537     110     605

  Fragments arranged by size: 

                2537     605     110
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2394    3252 
   Size:       2394     858
 
HaeII r_GCGC'y

Cuts at:      0     112     232     556     884    1743    3087    3252 
   Size:        112     120     324     328     859    1344     165

  Fragments arranged by size: 

                1344     859     328     324     165     120     112
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1330    1362    3117    3149    3252 
   Size:       1330      32    1755      32     103

  Fragments arranged by size: 

                1755    1330     103      32      32
 
HincII GTy'rAC

Cuts at:      0     665    2670    3252 
   Size:        665    2005     582

  Fragments arranged by size: 

                2005     665     582
 
HindIII A'AGCT_T

Cuts at:      0    2467    3252 
   Size:       2467     785
 
KpnI G_GTAC'C

Cuts at:      0    2865    3252 
   Size:       2865     387
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     833    3252 
   Size:        833    2419
 
MscI TGG'CCA

Cuts at:      0     103     358     499    2356    3252 
   Size:        103     255     141    1857     896

  Fragments arranged by size: 

                1857     896     255     141     103
 
MslI CAynn'nnrTG

Cuts at:      0      20     508    1852    1981    2425    3172    3252 
   Size:         20     488    1344     129     444     747      80

  Fragments arranged by size: 

                1344     747     488     444     129      80      20
 
MspA1I CmG'CkG

Cuts at:      0     210     393    1407    1800    1812    2105    2621    2877 
   Size:        210     183    1014     393      12     293     516     256

Cuts at:   2877    3112    3252 
   Size:        235     140

  Fragments arranged by size: 

                1014     516     393     293     256     235     210     183
                 140      12
 
NaeI GCC'GGC

Cuts at:      0     191    1271    1662    1845    3252 
   Size:        191    1080     391     183    1407

  Fragments arranged by size: 

                1407    1080     391     191     183
 
NcoI C'CATG_G

Cuts at:      0     143    1832    2426    2526    2702    3047    3173    3230 
   Size:        143    1689     594     100     176     345     126      57

Cuts at:   3230    3252 
   Size:         22

  Fragments arranged by size: 

                1689     594     345     176     143     126     100      57
                  22
 
NdeI CA'TA_TG

Cuts at:      0    2281    3252 
   Size:       2281     971
 
NgoMIV G'CCGG_C

Cuts at:      0     189    1269    1660    1843    3252 
   Size:        189    1080     391     183    1409

  Fragments arranged by size: 

                1409    1080     391     189     183
 
NspI r_CATG'y

Cuts at:      0      84    2580    2963    3252 
   Size:         84    2496     383     289

  Fragments arranged by size: 

                2496     383     289      84
 
PciI A'CATG_T

Cuts at:      0    2576    3252 
   Size:       2576     676
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      48    3252 
   Size:         48    3204
 
PfoI T'CCnGG_A

Cuts at:      0     919    3252 
   Size:        919    2333
 
PpuMI rG'GwC_Cy

Cuts at:      0    2537    3252 
   Size:       2537     715
 
PspOMI G'GGCC_C

Cuts at:      0    2647    3252 
   Size:       2647     605
 
PspXI vC'TCGA_Gb

Cuts at:      0    2914    3252 
   Size:       2914     338
 
PstI C_TGCA'G

Cuts at:      0     215     458    1244    1433    1508    2069    2477    3095 
   Size:        215     243     786     189      75     561     408     618

Cuts at:   3095    3252 
   Size:        157

  Fragments arranged by size: 

                 786     618     561     408     243     215     189     157
                  75
 
PvuI CG_AT'CG

Cuts at:      0    2753    3252 
   Size:       2753     499
 
PvuII CAG'CTG

Cuts at:      0     210    1812    2877    3252 
   Size:        210    1602    1065     375

  Fragments arranged by size: 

                1602    1065     375     210
 
SacI G_AGCT'C

Cuts at:      0       7     224    1586    3252 
   Size:          7     217    1362    1666

  Fragments arranged by size: 

                1666    1362     217       7
 
SacII CC_GC'GG

Cuts at:      0    2106    3252 
   Size:       2106    1146
 
SalI G'TCGA_C

Cuts at:      0     663    3252 
   Size:        663    2589
 
SanDI GG'GwC_CC

Cuts at:      0    2537    3252 
   Size:       2537     715
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1771    3252 
   Size:       1771    1481
 
SbfI CC_TGCA'GG

Cuts at:      0    2069    3252 
   Size:       2069    1183
 
ScaI AGT'ACT

Cuts at:      0    2719    3079    3252 
   Size:       2719     360     173

  Fragments arranged by size: 

                2719     360     173
 
SexAI A'CCwGG_T

Cuts at:      0    1436    3252 
   Size:       1436    1816
 
SfcI C'TryA_G

Cuts at:      0     211     454    1240    1429    1504    2065    2131    2473 
   Size:        211     243     786     189      75     561      66     342

Cuts at:   2473    2553    3091    3252 
   Size:         80     538     161

  Fragments arranged by size: 

                 786     561     538     342     243     211     189     161
                  80      75      66
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2683    3252 
   Size:       2683     569
 
SmaI CCC'GGG

Cuts at:      0    1885    3252 
   Size:       1885    1367
 
SmlI C'TyrA_G

Cuts at:      0     232     463    2914    3252 
   Size:        232     231    2451     338

  Fragments arranged by size: 

                2451     338     232     231
 
StuI AGG'CCT

Cuts at:      0    1295    1875    1964    3252 
   Size:       1295     580      89    1288

  Fragments arranged by size: 

                1295    1288     580      89
 
StyI C'CwwG_G

Cuts at:      0     143    1202    1325    1832    2426    2526    2702    3047 
   Size:        143    1059     123     507     594     100     176     345

Cuts at:   3047    3173    3230    3252 
   Size:        126      57      22

  Fragments arranged by size: 

                1059     594     507     345     176     143     126     123
                 100      57      22
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     921    1186    3252 
   Size:        921     265    2066

  Fragments arranged by size: 

                2066     921     265
 
TatI w'GTAC_w

Cuts at:      0    2573    2717    3077    3252 
   Size:       2573     144     360     175

  Fragments arranged by size: 

                2573     360     175     144
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     568     600    1295    1306    1327    1338    3252 
   Size:        568      32     695      11      21      11    1914

  Fragments arranged by size: 

                1914     695     568      32      21      11      11
 
Tth111I GACn'n_nGTC

Cuts at:      0    1395    3252 
   Size:       1395    1857
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1062    3252 
   Size:       1062    2190
 
XhoI C'TCGA_G

Cuts at:      0    2914    3252 
   Size:       2914     338
 
XmaI C'CCGG_G

Cuts at:      0    1883    3252 
   Size:       1883    1369
 
XmnI GAAnn'nnTTC

Cuts at:      0    2608    3252 
   Size:       2608     644
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI     PfoI    PpuMI   PspOMI
    PspXI     PstI     PvuI    PvuII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI    SgrAI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AlfI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI     BmtI    BsaAI    BsiWI     BsmI    BspHI    BsrDI
   BssHII    BstBI  BstZ17I   Bsu36I     ClaI     DraI   DraIII    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     MfeI
     MluI     NarI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI    RsrII     SfiI
     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     ZraI
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