Restriction map for pF1KSDB0018
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AcsI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
BalI        BcgI        BclI        BfrI        BglII       
BlpI        Bpu1102I    BsiEI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KspI        
MfeI        MluI        MluNI       MscI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       PvuI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tsp509I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        316  GGTACC               G^GTACC
AccI         1903  GTCTAC               GT^[AC][GT]AC
AccII         127  CGCG                 CG^CG
AclI          208  AACGTT               AA^CGTT
AflIII        759  ACGTGT               A^C[GA][CT]GT
ApaI         2104  GGGCCC               GGGCC^C
Asp718I       316  GGTACC               G^GTACC
AspI          334  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         419  TGCGCA               TGC^GCA
AvrII          39  CCTAGG               C^CTAGG
BamHI         734  GGATCC               G^GATCC
BbrPI         651  CACGTG               CAC^GTG
BciVI          59  GTATCC               GTATCC(6/5)
BlnI           39  CCTAGG               C^CTAGG
BsmBI         400  GAGACG               CGTCTC(1/5)
BspHI        1263  TCATGA               T^CATGA
BsrDI         296  GCAATG               GCAATG(2/0)
BssSI         502  CTCGTG               CACGAG(-5/-1)
BstUI         127  CGCG                 CG^CG
DrdI         1693  GACATCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          671  CCATGG               C^C[GA][CT]GG
EcoRV        1347  GATATC               GAT^ATC
FspI          419  TGCGCA               TGC^GCA
KpnI          316  GGTACC               GGTAC^C
MvnI          127  CGCG                 CG^CG
NcoI          671  CCATGG               C^CATGG
NruI          126  TCGCGA               TCG^CGA
NspI         2257  GCATGT               [GA]CATG^[CT]
PflFI         334  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         651  CACGTG               CAC^GTG
PmlI          651  CACGTG               CAC^GTG
Psp1406I      208  AACGTT               AA^CGTT
PstI          166  CTGCAG               CTGCA^G
RcaI         1263  TCATGA               T^CATGA
SbfI          165  CCTGCAGG             CCTGCA^GG
Sse8387I      165  CCTGCAGG             CCTGCA^GG
Tth111I       334  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1961  CCATCCAAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AvaI          278  CCCGGG               C^[CT]CG[GA]G
             2483  CCCGGG              
BanII         945  GAGCCC               G[GA]GC[CT]^C
             2104  GGGCCC              
BbeI          492  GGCGCC               GGCGC^C
              590  GGCGCC              
BbvCI         241  GCTGAGG              CCTCAGC(-5/-2)
              996  GCTGAGG             
BfaI           40  CTAG                 C^TAG
             1843  CTAG                
BsaAI         651  CACGTG               [CT]AC^GT[GA]
              758  TACGTG              
BsaBI         372  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1506  GATCCCCATC          
BsoBI         278  CCCGGG               C^[CT]CG[GA]G
             2483  CCCGGG              
BspMI         249  ACCTGC               ACCTGC(4/8)
              168  GCAGGT              
BsrBI         936  CCGCTC               CCGCTC(-3/-3)
             2546  CCGCTC              
BstXI        1061  CCAGAGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2387  CCAGGCCACTGG        
BstYI         734  GGATCC               [GA]^GATC[CT]
             1505  AGATCC              
EarI          981  CTCTTC               CTCTTC(1/4)
             1813  GAAGAG              
HphI          706  GGTGA                GGTGA(8/7)
             2506  GGTGA               
KasI          492  GGCGCC               G^GCGCC
              590  GGCGCC              
Ksp632I       981  CTCTTC               CTCTTC(1/4)
             1813  GAAGAG              
MaeI           40  CTAG                 C^TAG
             1843  CTAG                
MamI          372  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1506  GATCCCCATC          
MflI          734  GGATCC               [GA]^GATC[CT]
             1505  AGATCC              
MseI         1997  TTAA                 T^TAA
             2600  TTAA                
MslI          498  CACACTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2139  CACCCACATG          
NarI          492  GGCGCC               GG^CGCC
              590  GGCGCC              
SfaNI         377  GCATC                GCATC(5/9)
             2479  GCATC               
SfoI          492  GGCGCC               GGC^GCC
              590  GGCGCC              
SmaI          278  CCCGGG               CCC^GGG
             2483  CCCGGG              
StuI         1494  AGGCCT               AGG^CCT
             2359  AGGCCT              
Tru9I        1997  TTAA                 T^TAA
             2600  TTAA                
XhoII         734  GGATCC               [GA]^GATC[CT]
             1505  AGATCC              
XmaCI         278  CCCGGG               C^CCGGG
             2483  CCCGGG              
XmaI          278  CCCGGG               C^CCGGG
             2483  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccIII      AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        AvaII       BanI        
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspEI       
BsrI        BssKI       BstF5I      BstNI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       Eco81I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MroI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
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