Restriction map for pF1KSDB0018
 (Linear) (Six-base) MAPSORT of: KIBB0018.seq  Check: 1367  from: 1  to: 2640



KIBB0018.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     159     258    2640 
   Size:        159      99    2382

  Fragments arranged by size: 

                2382     159      99
 
AccI GT'mk_AC

Cuts at:      0    1904    2640 
   Size:       1904     736
 
Acc65I G'GTAC_C

Cuts at:      0     316    2640 
   Size:        316    2324
 
AclI AA'CG_TT

Cuts at:      0     209    2640 
   Size:        209    2431
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874     968    2341    2483    2640 
   Size:        874      94    1373     142     157

  Fragments arranged by size: 

                1373     874     157     142      94
 
AflIII A'CryG_T

Cuts at:      0     759    2640 
   Size:        759    1881
 
AleI CACnn'nnGTG

Cuts at:      0     502    2640 
   Size:        502    2138
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90     174    1468    1689    2640 
   Size:         90      84    1294     221     951

  Fragments arranged by size: 

                1294     951     221      90      84
 
ApaI G_GGCC'C

Cuts at:      0    2108    2640 
   Size:       2108     532
 
AvaI C'yCGr_G

Cuts at:      0     278    2483    2640 
   Size:        278    2205     157

  Fragments arranged by size: 

                2205     278     157
 
AvrII C'CTAG_G

Cuts at:      0      39    2640 
   Size:         39    2601
 
BamHI G'GATC_C

Cuts at:      0     734    2640 
   Size:        734    1906
 
BanI G'GyrC_C

Cuts at:      0     246     316     492     590    1071    1085    2137    2640 
   Size:        246      70     176      98     481      14    1052     503

  Fragments arranged by size: 

                1052     503     481     246     176      98      70      14
            
 
BanII G_rGCy'C

Cuts at:      0     949    2108    2640 
   Size:        949    1159     532

  Fragments arranged by size: 

                1159     949     532
 
BbeI G_GCGC'C

Cuts at:      0     496     594    2640 
   Size:        496      98    2046

  Fragments arranged by size: 

                2046     496      98
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     113     976    2397    2640 
   Size:        113     863    1421     243

  Fragments arranged by size: 

                1421     863     243     113
 
BbvCI CC'TCA_GC

Cuts at:      0     242     997    2640 
   Size:        242     755    1643

  Fragments arranged by size: 

                1643     755     242
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      70    2640 
   Size:         70    2570
 
BglI GCCn_nnn'nGGC

Cuts at:      0     238     588    1330    2020    2640 
   Size:        238     350     742     690     620

  Fragments arranged by size: 

                 742     690     620     350     238
 
Bme1580I G_kGCm'C

Cuts at:      0     658    1873    2108    2140    2640 
   Size:        658    1215     235      32     500

  Fragments arranged by size: 

                1215     658     500     235      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     652     793    1146    2640 
   Size:        652     141     353    1494

  Fragments arranged by size: 

                1494     652     353     141
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     475     532    1044    2558    2640 
   Size:        112     363      57     512    1514      82

  Fragments arranged by size: 

                1514     512     363     112      82      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     242     997    1934    1976    2042    2640 
   Size:        242     755     937      42      66     598

  Fragments arranged by size: 

                 937     755     598     242      66      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     294    1103    1172    1592    2322    2345    2640 
   Size:        294     809      69     420     730      23     295

  Fragments arranged by size: 

                 809     730     420     295     294      69      23
 
BsaAI yAC'GTr

Cuts at:      0     653     760    2640 
   Size:        653     107    1880

  Fragments arranged by size: 

                1880     653     107
 
BsaBI GATnn'nnATC

Cuts at:      0     376    1510    2640 
   Size:        376    1134    1130

  Fragments arranged by size: 

                1134    1130     376
 
BsaHI Gr'CG_yC

Cuts at:      0     493     591    1292    2640 
   Size:        493      98     701    1348

  Fragments arranged by size: 

                1348     701     493      98
 
BsaWI w'CCGG_w

Cuts at:      0     731     740    2285    2640 
   Size:        731       9    1545     355

  Fragments arranged by size: 

                1545     731     355       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1301    1331    1450    1480    1741    1771    1845    1875 
   Size:       1301      30     119      30     261      30      74      30

Cuts at:   1875    2486    2516    2640 
   Size:        611      30     124

  Fragments arranged by size: 

                1301     611     261     124     119      74      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     529     532     541     544    1225    1831    2524    2527 
   Size:        529       3       9       3     681     606     693       3

Cuts at:   2527    2530    2545    2638    2640 
   Size:          3      15      93       2

  Fragments arranged by size: 

                 693     681     606     529      93      15       9       3
                   3       3       3       2
 
BseYI C'CCAG_C

Cuts at:      0       5     308     459     773     914    1129    1144    1280 
   Size:          5     303     151     314     141     215      15     136

Cuts at:   1280    1389    1636    1921    2457    2640 
   Size:        109     247     285     536     183

  Fragments arranged by size: 

                 536     314     303     285     247     215     183     151
                 141     136     109      15       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     526     633     649     682     804     895    2640 
   Size:         75     451     107      16      33     122      91    1745

  Fragments arranged by size: 

                1745     451     122     107      91      75      33      16
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     394    2640 
   Size:        394    2246
 
Bsp1286I G_dGCh'C

Cuts at:      0     658     949    1873    2108    2140    2640 
   Size:        658     291     924     235      32     500

  Fragments arranged by size: 

                 924     658     500     291     235      32
 
BspEI T'CCGG_A

Cuts at:      0     731     740    2285    2640 
   Size:        731       9    1545     355

  Fragments arranged by size: 

                1545     731     355       9
 
BspHI T'CATG_A

Cuts at:      0    1263    2640 
   Size:       1263    1377
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     159     258    2640 
   Size:        159      99    2382

  Fragments arranged by size: 

                2382     159      99
 
BsrBI CCG'CTC

Cuts at:      0     938    2548    2640 
   Size:        938    1610      92

  Fragments arranged by size: 

                1610     938      92
 
BsrDI GCAATG_nn'

Cuts at:      0     303    2640 
   Size:        303    2337
 
BssSI C'ACGA_G

Cuts at:      0     502    2640 
   Size:        502    2138
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1068    2394    2640 
   Size:       1068    1326     246

  Fragments arranged by size: 

                1326    1068     246
 
BstYI r'GATC_y

Cuts at:      0     734    1505    2640 
   Size:        734     771    1135

  Fragments arranged by size: 

                1135     771     734
 
Bsu36I CC'TnA_GG

Cuts at:      0    1081    1408    2162    2621    2640 
   Size:       1081     327     754     459      19

  Fragments arranged by size: 

                1081     754     459     327      19
 
BtgI C'CryG_G

Cuts at:      0     671    2640 
   Size:        671    1969
 
BtsI GCAGTG_nn'

Cuts at:      0     645    1902    1933    2640 
   Size:        645    1257      31     707

  Fragments arranged by size: 

                1257     707     645      31
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1699    2640 
   Size:       1699     941
 
EarI CTCTTCn'nnn_

Cuts at:      0     987    1808    2640 
   Size:        987     821     832

  Fragments arranged by size: 

                 987     832     821
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     475     532     874     968    1044    2341    2483 
   Size:        112     363      57     342      94      76    1297     142

Cuts at:   2483    2558    2640 
   Size:         75      82

  Fragments arranged by size: 

                1297     363     342     142     112      94      82      76
                  75      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      14     241    1490    1813    2165    2640 
   Size:         14     227    1249     323     352     475

  Fragments arranged by size: 

                1249     475     352     323     227      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0      35    1001    1055    1728    1945    1982    2154    2211 
   Size:         35     966      54     673     217      37     172      57

Cuts at:   2211    2301    2580    2640 
   Size:         90     279      60

  Fragments arranged by size: 

                 966     673     279     217     172      90      60      57
                  54      37      35
 
EcoRV GAT'ATC

Cuts at:      0    1349    2640 
   Size:       1349    1291
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2629    2640 
   Size:       2629      11
 
FspI TGC'GCA

Cuts at:      0     421    2640 
   Size:        421    2219
 
HaeII r_GCGC'y

Cuts at:      0     480     496     583     594    2640 
   Size:        480      16      87      11    2046

  Fragments arranged by size: 

                2046     480      87      16      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357     889     921    1450    1482    1589    1621 
   Size:        325      32     532      32     529      32     107      32

Cuts at:   1621    1845    1877    2224    2256    2614    2640 
   Size:        224      32     347      32     358      26

  Fragments arranged by size: 

                 532     529     358     347     325     224     107      32
                  32      32      32      32      32      26
 
KasI G'GCGC_C

Cuts at:      0     492     590    2640 
   Size:        492      98    2050

  Fragments arranged by size: 

                2050     492      98
 
KpnI G_GTAC'C

Cuts at:      0     320    2640 
   Size:        320    2320
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    1504    2640 
   Size:        737     767    1136

  Fragments arranged by size: 

                1136     767     737
 
MslI CAynn'nnrTG

Cuts at:      0     502    2143    2640 
   Size:        502    1641     497

  Fragments arranged by size: 

                1641     502     497
 
MspA1I CmG'CkG

Cuts at:      0     510     818    1129    1280    1689    2050    2640 
   Size:        510     308     311     151     409     361     590

  Fragments arranged by size: 

                 590     510     409     361     311     308     151
 
NarI GG'CG_CC

Cuts at:      0     493     591    2640 
   Size:        493      98    2049

  Fragments arranged by size: 

                2049     493      98
 
NcoI C'CATG_G

Cuts at:      0     671    2640 
   Size:        671    1969
 
NruI TCG'CGA

Cuts at:      0     128    2640 
   Size:        128    2512
 
NspI r_CATG'y

Cuts at:      0    2261    2640 
   Size:       2261     379
 
PasI CC'CwG_GG

Cuts at:      0    1235    2640 
   Size:       1235    1405
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     753     795    1256    1689    1704    1971    2640 
   Size:        753      42     461     433      15     267     669

  Fragments arranged by size: 

                 753     669     461     433     267      42      15
 
PfoI T'CCnGG_A

Cuts at:      0    1421    2640 
   Size:       1421    1219
 
PmlI CAC'GTG

Cuts at:      0     653    2640 
   Size:        653    1987
 
PpuMI rG'GwC_Cy

Cuts at:      0      35    1728    1945    1982    2154    2301    2640 
   Size:         35    1693     217      37     172     147     339

  Fragments arranged by size: 

                1693     339     217     172     147      37      35
 
PspOMI G'GGCC_C

Cuts at:      0    2104    2640 
   Size:       2104     536
 
PstI C_TGCA'G

Cuts at:      0     170    2640 
   Size:        170    2470
 
PvuII CAG'CTG

Cuts at:      0     510    1129    1280    1689    2050    2640 
   Size:        510     619     151     409     361     590

  Fragments arranged by size: 

                 619     590     510     409     361     151
 
SanDI GG'GwC_CC

Cuts at:      0    1982    2301    2640 
   Size:       1982     319     339

  Fragments arranged by size: 

                1982     339     319
 
SbfI CC_TGCA'GG

Cuts at:      0     170    2640 
   Size:        170    2470
 
SfcI C'TryA_G

Cuts at:      0     166    1738    1803    2640 
   Size:        166    1572      65     837

  Fragments arranged by size: 

                1572     837     166      65
 
SfoI GGC'GCC

Cuts at:      0     494     592    2640 
   Size:        494      98    2048

  Fragments arranged by size: 

                2048     494      98
 
SmaI CCC'GGG

Cuts at:      0     280    2485    2640 
   Size:        280    2205     155

  Fragments arranged by size: 

                2205     280     155
 
StuI AGG'CCT

Cuts at:      0    1496    2361    2640 
   Size:       1496     865     279

  Fragments arranged by size: 

                1496     865     279
 
StyI C'CwwG_G

Cuts at:      0      39     671    1433    1491    1772    2132    2228    2252 
   Size:         39     632     762      58     281     360      96      24

Cuts at:   2252    2432    2640 
   Size:        180     208

  Fragments arranged by size: 

                 762     632     360     281     208     180      96      58
                  39      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     694    1004    1158    2155    2640 
   Size:        694     310     154     997     485

  Fragments arranged by size: 

                 997     694     485     310     154
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     684     716    1301    1333    2640 
   Size:        684      32     585      32    1307

  Fragments arranged by size: 

                1307     684     585      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     337    2640 
   Size:        337    2303
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1968    2640 
   Size:       1968     672
 
XmaI C'CCGG_G

Cuts at:      0     278    2483    2640 
   Size:        278    2205     157

  Fragments arranged by size: 

                2205     278     157
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI
     ApaI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BglI Bme1580I     BmrI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BssSI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FspI    HaeII    Hin4I     KasI
     KpnI     MmeI     MslI   MspA1I     NarI     NcoI     NruI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
    SanDI     SbfI     SfcI     SfoI     SmaI     StuI     StyI    TaqII
     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI     BaeI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI    EcoRI     FseI    FspAI   HincII  HindIII     HpaI
     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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