Restriction map for pF1KSDA2008
 (Linear) (Six-base) MAPSORT of: KIAA2008.seq  Check: 5028  from: 1  to: 2376



KIAA2008.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1885    2116    2175    2376 
   Size:       1885     231      59     201

  Fragments arranged by size: 

                1885     231     201      59
 
AatII G_ACGT'C

Cuts at:      0     118    2376 
   Size:        118    2258
 
AccI GT'mk_AC

Cuts at:      0    1790    1850    2376 
   Size:       1790      60     526

  Fragments arranged by size: 

                1790     526      60
 
Acc65I G'GTAC_C

Cuts at:      0    1035    2376 
   Size:       1035    1341
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     937    1030    1653    2268    2376 
   Size:        937      93     623     615     108

  Fragments arranged by size: 

                 937     623     615     108      93
 
AflIII A'CryG_T

Cuts at:      0     896    1778    2376 
   Size:        896     882     598

  Fragments arranged by size: 

                 896     882     598
 
AgeI A'CCGG_T

Cuts at:      0    1154    2376 
   Size:       1154    1222
 
AleI CACnn'nnGTG

Cuts at:      0     178    2376 
   Size:        178    2198
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     304    1672    2376 
   Size:        304    1368     704

  Fragments arranged by size: 

                1368     704     304
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414     813     825     858    1566    1569    1945    2376 
   Size:        414     399      12      33     708       3     376     431

  Fragments arranged by size: 

                 708     431     414     399     376      33      12       3
            
 
ApaI G_GGCC'C

Cuts at:      0     193     929    1618    2376 
   Size:        193     736     689     758

  Fragments arranged by size: 

                 758     736     689     193
 
AvaI C'yCGr_G

Cuts at:      0     193     908    1507    2008    2376 
   Size:        193     715     599     501     368

  Fragments arranged by size: 

                 715     599     501     368     193
 
AvrII C'CTAG_G

Cuts at:      0     930    2376 
   Size:        930    1446
 
BamHI G'GATC_C

Cuts at:      0     359    1898    2376 
   Size:        359    1539     478

  Fragments arranged by size: 

                1539     478     359
 
BanI G'GyrC_C

Cuts at:      0     143     474     574     663    1035    1157    1477    1512 
   Size:        143     331     100      89     372     122     320      35

Cuts at:   1512    2187    2376 
   Size:        675     189

  Fragments arranged by size: 

                 675     372     331     320     189     143     122     100
                  89      35
 
BanII G_rGCy'C

Cuts at:      0     193     929    1337    1618    1938    1978    2019    2138 
   Size:        193     736     408     281     320      40      41     119

Cuts at:   2138    2376 
   Size:        238

  Fragments arranged by size: 

                 736     408     320     281     238     193     119      41
                  40
 
BbeI G_GCGC'C

Cuts at:      0     147    2376 
   Size:        147    2229
 
BclI T'GATC_A

Cuts at:      0       2    2376 
   Size:          2    2374
 
BglI GCCn_nnn'nGGC

Cuts at:      0     661     793    1828    2068    2077    2376 
   Size:        661     132    1035     240       9     299

  Fragments arranged by size: 

                1035     661     299     240     132       9
 
Bme1580I G_kGCm'C

Cuts at:      0     193     531     666     929    1618    2192    2376 
   Size:        193     338     135     263     689     574     184

  Fragments arranged by size: 

                 689     574     338     263     193     184     135
 
BmgBI CAC'GTC

Cuts at:      0     897    2376 
   Size:        897    1479
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     473     783    1254    2376 
   Size:        473     310     471    1122

  Fragments arranged by size: 

                1122     473     471     310
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1752    2376 
   Size:       1752     624
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     292     657    1408    1648    1707    1912    1922 
   Size:        253      39     365     751     240      59     205      10

Cuts at:   1922    2376 
   Size:        454

  Fragments arranged by size: 

                 751     454     365     253     240     205      59      39
                  10
 
Bpu10I CC'TnA_GC

Cuts at:      0     504    2376 
   Size:        504    1872
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2161    2376 
   Size:       2161     215
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      51    2376 
   Size:         51    2325
 
BsaAI yAC'GTr

Cuts at:      0    1779    2376 
   Size:       1779     597
 
BsaHI Gr'CG_yC

Cuts at:      0     115     144     206    2168    2376 
   Size:        115      29      62    1962     208

  Fragments arranged by size: 

                1962     208     115      62      29
 
BsaWI w'CCGG_w

Cuts at:      0    1154    1235    2376 
   Size:       1154      81    1141

  Fragments arranged by size: 

                1154    1141      81
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     898    1225    1345    1822    2376 
   Size:        898     327     120     477     554

  Fragments arranged by size: 

                 898     554     477     327     120
 
BseYI C'CCAG_C

Cuts at:      0     806     825    1739    2240    2376 
   Size:        806      19     914     501     136

  Fragments arranged by size: 

                 914     806     501     136      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     295     418     950     964    1210    2082    2376 
   Size:        274      21     123     532      14     246     872     294

  Fragments arranged by size: 

                 872     532     294     274     246     123      21      14
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     586    1337    1938    2376 
   Size:        586     751     601     438

  Fragments arranged by size: 

                 751     601     586     438
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     122     387    1336    2376 
   Size:        122     265     949    1040

  Fragments arranged by size: 

                1040     949     265     122
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     531     586     666     929    1337    1618    1938 
   Size:        193     338      55      80     263     408     281     320

Cuts at:   1938    1978    2019    2138    2192    2376 
   Size:         40      41     119      54     184

  Fragments arranged by size: 

                 408     338     320     281     263     193     184     119
                  80      55      54      41      40
 
BspHI T'CATG_A

Cuts at:      0     758    1277    2376 
   Size:        758     519    1099

  Fragments arranged by size: 

                1099     758     519
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     236     317    1203    1885    2116    2175    2270    2376 
   Size:        236      81     886     682     231      59      95     106

  Fragments arranged by size: 

                 886     682     236     231     106      95      81      59
            
 
BsrBI CCG'CTC

Cuts at:      0     357    1062    1896    2376 
   Size:        357     705     834     480

  Fragments arranged by size: 

                 834     705     480     357
 
BsrFI r'CCGG_y

Cuts at:      0     298     343     455    1154    2293    2315    2376 
   Size:        298      45     112     699    1139      22      61

  Fragments arranged by size: 

                1139     699     298     112      61      45      22
 
BssSI C'ACGA_G

Cuts at:      0    1207    1696    2376 
   Size:       1207     489     680

  Fragments arranged by size: 

                1207     680     489
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     581    1569    2064    2376 
   Size:        581     988     495     312

  Fragments arranged by size: 

                 988     581     495     312
 
BstEII G'GTnAC_C

Cuts at:      0      48    2376 
   Size:         48    2328
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1383    2376 
   Size:       1383     993
 
BstYI r'GATC_y

Cuts at:      0     359     410     854    1469    1898    2376 
   Size:        359      51     444     615     429     478

  Fragments arranged by size: 

                 615     478     444     429     359      51
 
BtgI C'CryG_G

Cuts at:      0     201     984    1104    1388    1473    1533    2118    2376 
   Size:        201     783     120     284      85      60     585     258

  Fragments arranged by size: 

                 783     585     284     258     201     120      85      60
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     375    1620    2376 
   Size:        375    1245     756

  Fragments arranged by size: 

                1245     756     375
 
BtsI GCAGTG_nn'

Cuts at:      0     288     952    2376 
   Size:        288     664    1424

  Fragments arranged by size: 

                1424     664     288
 
DraIII CAC_nnn'GTG

Cuts at:      0     481     581    2376 
   Size:        481     100    1795

  Fragments arranged by size: 

                1795     481     100
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     885    2376 
   Size:        885    1491
 
EaeI y'GGCC_r

Cuts at:      0     263    1385    2078    2247    2376 
   Size:        263    1122     693     169     129

  Fragments arranged by size: 

                1122     693     263     169     129
 
EarI CTCTTCn'nnn_

Cuts at:      0    1591    2287    2376 
   Size:       1591     696      89

  Fragments arranged by size: 

                1591     696      89
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     967    2376 
   Size:        967    1409
 
EcoICRI GAG'CTC

Cuts at:      0    1335    1936    2376 
   Size:       1335     601     440

  Fragments arranged by size: 

                1335     601     440
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     292     657     937    1030    1408    1648    1653 
   Size:        253      39     365     280      93     378     240       5

Cuts at:   1653    1707    1912    1922    2268    2376 
   Size:         54     205      10     346     108

  Fragments arranged by size: 

                 378     365     346     280     253     240     205     108
                  93      54      39      10       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     604     880    1954    2266    2376 
   Size:        604     276    1074     312     110

  Fragments arranged by size: 

                1074     604     312     276     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     189     190     912     926    1614    1615    2042    2093 
   Size:        189       1     722      14     688       1     427      51

Cuts at:   2093    2376 
   Size:        283

  Fragments arranged by size: 

                 722     688     427     283     189      51      14       1
                   1
 
FseI GG_CCGG'CC

Cuts at:      0     347    2376 
   Size:        347    2029
 
FspI TGC'GCA

Cuts at:      0     214    1573    2376 
   Size:        214    1359     803

  Fragments arranged by size: 

                1359     803     214
 
HaeII r_GCGC'y

Cuts at:      0     147     799     813    2376 
   Size:        147     652      14    1563

  Fragments arranged by size: 

                1563     652     147      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32      64     102     134    2376 
   Size:         32      32      38      32    2242

  Fragments arranged by size: 

                2242      38      32      32      32
 
HincII GTy'rAC

Cuts at:      0      12    1791    2376 
   Size:         12    1779     585

  Fragments arranged by size: 

                1779     585      12
 
KasI G'GCGC_C

Cuts at:      0     143    2376 
   Size:        143    2233
 
KpnI G_GTAC'C

Cuts at:      0    1039    2376 
   Size:       1039    1337
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     410    2189    2324    2376 
   Size:        410    1779     135      52

  Fragments arranged by size: 

                1779     410     135      52
 
MscI TGG'CCA

Cuts at:      0     265    2249    2376 
   Size:        265    1984     127

  Fragments arranged by size: 

                1984     265     127
 
MslI CAynn'nnrTG

Cuts at:      0     178     394    1282    1387    2376 
   Size:        178     216     888     105     989

  Fragments arranged by size: 

                 989     888     216     178     105
 
MspA1I CmG'CkG

Cuts at:      0     203     533     630     737    1500    1566    2288    2376 
   Size:        203     330      97     107     763      66     722      88

  Fragments arranged by size: 

                 763     722     330     203     107      97      88      66
            
 
NaeI GCC'GGC

Cuts at:      0     300     345     457    2295    2317    2376 
   Size:        300      45     112    1838      22      59

  Fragments arranged by size: 

                1838     300     112      59      45      22
 
NarI GG'CG_CC

Cuts at:      0     144    2376 
   Size:        144    2232
 
NcoI C'CATG_G

Cuts at:      0     984    1473    2376 
   Size:        984     489     903

  Fragments arranged by size: 

                 984     903     489
 
NgoMIV G'CCGG_C

Cuts at:      0     298     343     455    2293    2315    2376 
   Size:        298      45     112    1838      22      61

  Fragments arranged by size: 

                1838     298     112      61      45      22
 
PasI CC'CwG_GG

Cuts at:      0     337    2376 
   Size:        337    2039
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     720     813    2376 
   Size:        720      93    1563

  Fragments arranged by size: 

                1563     720      93
 
PmlI CAC'GTG

Cuts at:      0    1779    2376 
   Size:       1779     597
 
PpuMI rG'GwC_Cy

Cuts at:      0     912    2376 
   Size:        912    1464
 
PspOMI G'GGCC_C

Cuts at:      0     189     925    1614    2376 
   Size:        189     736     689     762

  Fragments arranged by size: 

                 762     736     689     189
 
PspXI vC'TCGA_Gb

Cuts at:      0    2008    2376 
   Size:       2008     368
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2209    2218    2241    2250    2376 
   Size:       2209       9      23       9     126

  Fragments arranged by size: 

                2209     126      23       9       9
 
PstI C_TGCA'G

Cuts at:      0    1022    1115    1661    1933    2186    2267    2376 
   Size:       1022      93     546     272     253      81     109

  Fragments arranged by size: 

                1022     546     272     253     109      93      81
 
PvuII CAG'CTG

Cuts at:      0    1566    2288    2376 
   Size:       1566     722      88

  Fragments arranged by size: 

                1566     722      88
 
SacI G_AGCT'C

Cuts at:      0    1337    1938    2376 
   Size:       1337     601     438

  Fragments arranged by size: 

                1337     601     438
 
SacII CC_GC'GG

Cuts at:      0     204    2376 
   Size:        204    2172
 
SalI G'TCGA_C

Cuts at:      0    1789    2376 
   Size:       1789     587
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2287    2376 
   Size:       2287      89
 
SfcI C'TryA_G

Cuts at:      0    1018    1111    1657    1929    2182    2263    2376 
   Size:       1018      93     546     272     253      81     113

  Fragments arranged by size: 

                1018     546     272     253     113      93      81
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2077    2376 
   Size:       2077     299
 
SfoI GGC'GCC

Cuts at:      0     145    2376 
   Size:        145    2231
 
SmlI C'TyrA_G

Cuts at:      0    2008    2176    2376 
   Size:       2008     168     200

  Fragments arranged by size: 

                2008     200     168
 
StyI C'CwwG_G

Cuts at:      0     930     984    1473    2376 
   Size:        930      54     489     903

  Fragments arranged by size: 

                 930     903     489      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     492     681     850    2017    2376 
   Size:        492     189     169    1167     359

  Fragments arranged by size: 

                1167     492     359     189     169
 
TatI w'GTAC_w

Cuts at:      0    1484    1871    2376 
   Size:       1484     387     505

  Fragments arranged by size: 

                1484     505     387
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     959     991    2376 
   Size:        959      32    1385

  Fragments arranged by size: 

                1385     959      32
 
XhoI C'TCGA_G

Cuts at:      0    2008    2376 
   Size:       2008     368
 
ZraI GAC'GTC

Cuts at:      0     116    2376 
   Size:        116    2260
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BclI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     PasI    PflMI     PmlI    PpuMI   PspOMI    PspXI     PsrI     PstI
    PvuII     SacI    SacII     SalI     SapI     SfcI     SfiI     SfoI
     SmlI     StyI    TaqII     TatI     TstI     XhoI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AhdI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI     BaeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
    BglII     BlpI     BmtI    BsaBI    BsaXI    BsiEI    BsiWI     BsmI
    BspEI    BsrDI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     EagI    EcoRI    EcoRV     FalI    FspAI  HindIII
     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI
     PvuI    RsrII    SanDI     SbfI     ScaI    SexAI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XmaI     XmnI
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