Restriction map for pF1KSDA1950
 (Linear) (Six-base) MAPSORT of: KIAA1950.seq  Check: 5781  from: 1  to: 1494



KIAA1950.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1275    1494 
   Size:       1275     219
 
AccI GT'mk_AC

Cuts at:      0      12    1494 
   Size:         12    1482
 
Acc65I G'GTAC_C

Cuts at:      0     941    1494 
   Size:        941     553
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     561     723    1494 
   Size:        561     162     771

  Fragments arranged by size: 

                 771     561     162
 
AleI CACnn'nnGTG

Cuts at:      0    1087    1494 
   Size:       1087     407
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90     725    1251    1494 
   Size:         90     635     526     243

  Fragments arranged by size: 

                 635     526     243      90
 
ApaI G_GGCC'C

Cuts at:      0      41     656     732    1198    1494 
   Size:         41     615      76     466     296

  Fragments arranged by size: 

                 615     466     296      76      41
 
ApoI r'AATT_y

Cuts at:      0     637    1494 
   Size:        637     857
 
AvaI C'yCGr_G

Cuts at:      0      41     211     220     700    1047    1494 
   Size:         41     170       9     480     347     447

  Fragments arranged by size: 

                 480     447     347     170      41       9
 
BanI G'GyrC_C

Cuts at:      0     941    1081    1494 
   Size:        941     140     413

  Fragments arranged by size: 

                 941     413     140
 
BanII G_rGCy'C

Cuts at:      0      41     656     732     788    1079    1100    1198    1494 
   Size:         41     615      76      56     291      21      98     296

  Fragments arranged by size: 

                 615     296     291      98      76      56      41      21
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     171     205    1494 
   Size:        171      34    1289

  Fragments arranged by size: 

                1289     171      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1174    1494 
   Size:       1174     320
 
BlpI GC'TnA_GC

Cuts at:      0     847    1153    1494 
   Size:        847     306     341

  Fragments arranged by size: 

                 847     341     306
 
Bme1580I G_kGCm'C

Cuts at:      0      41     599     656     732     747    1198    1440    1494 
   Size:         41     558      57      76      15     451     242      54

  Fragments arranged by size: 

                 558     451     242      76      57      54      41      15
            
 
BmgBI CAC'GTC

Cuts at:      0     983    1494 
   Size:        983     511
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1086    1494 
   Size:       1086     408
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     585    1494 
   Size:        442     143     909

  Fragments arranged by size: 

                 909     442     143
 
Bpu10I CC'TnA_GC

Cuts at:      0     202     307    1494 
   Size:        202     105    1187

  Fragments arranged by size: 

                1187     202     105
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      69    1494 
   Size:         69    1425
 
BsaAI yAC'GTr

Cuts at:      0     776    1494 
   Size:        776     718
 
BsaHI Gr'CG_yC

Cuts at:      0     608    1304    1494 
   Size:        608     696     190

  Fragments arranged by size: 

                 696     608     190
 
BsaWI w'CCGG_w

Cuts at:      0     257    1494 
   Size:        257    1237
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1396    1426    1494 
   Size:       1396      30      68

  Fragments arranged by size: 

                1396      68      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     328     417    1285    1404    1437    1494 
   Size:        328      89     868     119      33      57

  Fragments arranged by size: 

                 868     328     119      89      57      33
 
BseYI C'CCAG_C

Cuts at:      0     436     525     655     725     988    1316    1363    1398 
   Size:        436      89     130      70     263     328      47      35

Cuts at:   1398    1494 
   Size:         96

  Fragments arranged by size: 

                 436     328     263     130      96      89      70      47
                  35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     322     339     369    1303    1494 
   Size:        150     172      17      30     934     191

  Fragments arranged by size: 

                 934     191     172     150      30      17
 
BsiEI CG_ry'CG

Cuts at:      0    1314    1494 
   Size:       1314     180
 
BsiHKAI G_wGCw'C

Cuts at:      0     788    1494 
   Size:        788     706
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     599     656     732     747     788    1079    1100 
   Size:         41     558      57      76      15      41     291      21

Cuts at:   1100    1198    1440    1494 
   Size:         98     242      54

  Fragments arranged by size: 

                 558     291     242      98      76      57      54      41
                  41      21      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1275    1494 
   Size:       1275     219
 
BsrBI CCG'CTC

Cuts at:      0     120    1494 
   Size:        120    1374
 
BsrFI r'CCGG_y

Cuts at:      0     318    1494 
   Size:        318    1176
 
BssSI C'ACGA_G

Cuts at:      0     781    1494 
   Size:        781     713
 
Bsu36I CC'TnA_GG

Cuts at:      0     898    1134    1420    1494 
   Size:        898     236     286      74

  Fragments arranged by size: 

                 898     286     236      74
 
BtgI C'CryG_G

Cuts at:      0    1298    1494 
   Size:       1298     196
 
BtsI GCAGTG_nn'

Cuts at:      0      13    1494 
   Size:         13    1481
 
DraIII CAC_nnn'GTG

Cuts at:      0     603    1494 
   Size:        603     891
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      61    1494 
   Size:         61    1433
 
EaeI y'GGCC_r

Cuts at:      0     131    1232    1343    1494 
   Size:        131    1101     111     151

  Fragments arranged by size: 

                1101     151     131     111
 
EarI CTCTTCn'nnn_

Cuts at:      0     130     166    1481    1494 
   Size:        130      36    1315      13

  Fragments arranged by size: 

                1315     130      36      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      90    1494 
   Size:         90    1404
 
EcoICRI GAG'CTC

Cuts at:      0     786    1494 
   Size:        786     708
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     561     585     723    1494 
   Size:        442     119      24     138     771

  Fragments arranged by size: 

                 771     442     138     119      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133     310     679     835     856    1282    1419    1494 
   Size:        133     177     369     156      21     426     137      75

  Fragments arranged by size: 

                 426     369     177     156     137     133      75      21
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38     652     705     729     861    1194    1195    1248 
   Size:         38     614      53      24     132     333       1      53

Cuts at:   1248    1275    1356    1494 
   Size:         27      81     138

  Fragments arranged by size: 

                 614     333     138     132      81      53      53      38
                  27      24       1
 
EcoRI G'AATT_C

Cuts at:      0     637    1494 
   Size:        637     857
 
FspI TGC'GCA

Cuts at:      0    1390    1494 
   Size:       1390     104
 
HaeII r_GCGC'y

Cuts at:      0     662    1043    1494 
   Size:        662     381     451

  Fragments arranged by size: 

                 662     451     381
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1396    1428    1494 
   Size:       1396      32      66

  Fragments arranged by size: 

                1396      66      32
 
KpnI G_GTAC'C

Cuts at:      0     945    1494 
   Size:        945     549
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1073    1494 
   Size:       1073     421
 
MscI TGG'CCA

Cuts at:      0    1234    1494 
   Size:       1234     260
 
MslI CAynn'nnrTG

Cuts at:      0    1087    1494 
   Size:       1087     407
 
MspA1I CmG'CkG

Cuts at:      0      90     345     353     422     818     827    1182    1257 
   Size:         90     255       8      69     396       9     355      75

Cuts at:   1257    1316    1494 
   Size:         59     178

  Fragments arranged by size: 

                 396     355     255     178      90      75      69      59
                   9       8
 
NspI r_CATG'y

Cuts at:      0     916    1056    1494 
   Size:        916     140     438

  Fragments arranged by size: 

                 916     438     140
 
PasI CC'CwG_GG

Cuts at:      0     747    1475    1494 
   Size:        747     728      19

  Fragments arranged by size: 

                 747     728      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     798    1494 
   Size:        798     696
 
PmlI CAC'GTG

Cuts at:      0     776    1494 
   Size:        776     718
 
PpuMI rG'GwC_Cy

Cuts at:      0    1275    1356    1494 
   Size:       1275      81     138

  Fragments arranged by size: 

                1275     138      81
 
PshAI GACnn'nnGTC

Cuts at:      0     884    1494 
   Size:        884     610
 
PspOMI G'GGCC_C

Cuts at:      0      37     652     728    1194    1494 
   Size:         37     615      76     466     300

  Fragments arranged by size: 

                 615     466     300      76      37
 
PstI C_TGCA'G

Cuts at:      0       8      86     254     616     716     911    1256    1494 
   Size:          8      78     168     362     100     195     345     238

  Fragments arranged by size: 

                 362     345     238     195     168     100      78       8
            
 
PvuII CAG'CTG

Cuts at:      0      90     345     353    1494 
   Size:         90     255       8    1141

  Fragments arranged by size: 

                1141     255      90       8
 
SacI G_AGCT'C

Cuts at:      0     788    1494 
   Size:        788     706
 
SapI GCTCTTCn'nnn_

Cuts at:      0     130     166    1494 
   Size:        130      36    1328

  Fragments arranged by size: 

                1328     130      36
 
SbfI CC_TGCA'GG

Cuts at:      0     716    1494 
   Size:        716     778
 
SexAI A'CCwGG_T

Cuts at:      0    1277    1494 
   Size:       1277     217
 
SfcI C'TryA_G

Cuts at:      0       4      82     250     292     612     712     907    1252 
   Size:          4      78     168      42     320     100     195     345

Cuts at:   1252    1494 
   Size:        242

  Fragments arranged by size: 

                 345     320     242     195     168     100      78      42
                   4
 
SmaI CCC'GGG

Cuts at:      0      43    1494 
   Size:         43    1451
 
StuI AGG'CCT

Cuts at:      0     139     364     562    1494 
   Size:        139     225     198     932

  Fragments arranged by size: 

                 932     225     198     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1377    1494 
   Size:       1377     117
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     502     534    1419    1451    1494 
   Size:        502      32     885      32      43

  Fragments arranged by size: 

                 885     502      43      32      32
 
XmaI C'CCGG_G

Cuts at:      0      41    1494 
   Size:         41    1453
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AleI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BcgI     BglI     BlpI Bme1580I    BmgBI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI
    BsrFI    BssSI   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     NspI
     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI     SapI     SbfI    SexAI     SfcI     SmaI     StuI    TaqII
     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BmrI
     BmtI    BpuEI    BsaBI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     ClaI    CspCI     DraI     EagI    EcoRV     FalI
     FseI    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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