Restriction map for pF1KSDA1820
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
ApoI        AscI        AseI        AsnI        Asp700I     
AspI        AviII       AvrII       BbrPI       BciVI       
BclI        BfrI        BlnI        BsaAI       BsaBI       
BsiWI       BsmBI       Bsp1407I    BspDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HpaI        MamI        MfeI        
MluI        MslI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SalI        SapI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccIII        983  TCCGGA               T^CCGGA
AflIII       1688  ACATGT               A^C[GA][CT]GT
AgeI         2504  ACCGGT               A^CCGGT
AocI         3787  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      3422  AGCGCT               AGC^GCT
BamHI        4704  GGATCC               G^GATCC
BfaI           79  CTAG                 C^TAG
BglII        3461  AGATCT               A^GATCT
BseAI         983  TCCGGA               T^CCGGA
BsmI          633  GCATTC               GAATGC(1/-1)
BspEI         983  TCCGGA               T^CCGGA
BspHI        4235  TCATGA               T^CATGA
BspLU11I     1688  ACATGT               A^CATGT
BssSI        1402  CTCGTG               CACGAG(-5/-1)
Bsu36I       3787  CCTGAGG              CC^T[ACGT]AGG
DrdI          973  GACGCAGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3335  CGGCCG               C^GGCCG
EclXI        3335  CGGCCG               C^GGCCG
Eco47III     3422  AGCGCT               AGC^GCT
Eco52I       3335  CGGCCG               C^GGCCG
Eco81I       3787  CCTGAGG              CC^T[ACGT]AGG
HincII       1624  GTCAAC               GT[CT]^[GA]AC
HindII       1624  GTCAAC               GT[CT]^[GA]AC
MaeI           79  CTAG                 C^TAG
MaeII         515  ACGT                 A^CGT
MroI          983  TCCGGA               T^CCGGA
MseI         4222  TTAA                 T^TAA
PflMI        4174  CCAACTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2504  ACCGGT               A^CCGGT
RcaI         4235  TCATGA               T^CATGA
Tru9I        4222  TTAA                 T^TAA
Tsp509I        85  AATT                 ^AATT
Van91I       4174  CCAACTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I        616  GGTACC               G^GTACC
             2125  GGTACC              
Alw44I       3007  GTGCAC               G^TGCAC
             4869  GTGCAC              
ApaLI        3007  GTGCAC               G^TGCAC
             4869  GTGCAC              
Asp718I       616  GGTACC               G^GTACC
             2125  GGTACC              
BcgI         4861  CGACCGTTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
                3  GCAGTCTTTTCG        
BsaI         3886  GAGACC               GGTCTC(1/5)
             3564  GGTCTC              
BsaWI         983  TCCGGA               [AT]^CCGG[AT]
             2504  ACCGGT              
HindIII      1871  AAGCTT               A^AGCTT
             2988  AAGCTT              
KpnI          616  GGTACC               GGTAC^C
             2125  GGTACC              
KspI          212  CCGCGG               CCGC^GG
             1783  CCGCGG              
NcoI           30  CCATGG               C^CATGG
             4365  CCATGG              
PvuII        1351  CAGCTG               CAG^CTG
             2014  CAGCTG              
SacI         2626  GAGCTC               GAGCT^C
             2879  GAGCTC              
SacII         212  CCGCGG               CCGC^GG
             1783  CCGCGG              
SbfI          270  CCTGCAGG             CCTGCA^GG
              403  CCTGCAGG            
ScaI          998  AGTACT               AGT^ACT
             2609  AGTACT              
SexAI        3621  ACCTGGT              A^CC[AT]GGT
             3713  ACCTGGT             
SmaI          774  CCCGGG               CCC^GGG
             2419  CCCGGG              
SphI         1524  GCATGC               GCATG^C
             2657  GCATGC              
Sse8387I      270  CCTGCAGG             CCTGCA^GG
              403  CCTGCAGG            
XmaCI         774  CCCGGG               C^CCGGG
             2419  CCCGGG              
XmaI          774  CCCGGG               C^CCGGG
             2419  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApaI        AspEI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        XhoII       
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