Restriction map for pF1KSDA1734
 (Linear) (Six-base) MAPSORT of: KIAA1734.seq  Check: 1466  from: 1  to: 3876



KIAA1734.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AatII G_ACGT'C

Cuts at:      0     506     728    2057    3876 
   Size:        506     222    1329    1819

  Fragments arranged by size: 

                1819    1329     506     222
 
AccI GT'mk_AC

Cuts at:      0     638    1808    3876 
   Size:        638    1170    2068

  Fragments arranged by size: 

                2068    1170     638
 
Acc65I G'GTAC_C

Cuts at:      0     377    3876 
   Size:        377    3499
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685    2847    3876 
   Size:        685    2162    1029

  Fragments arranged by size: 

                2162    1029     685
 
AfeI AGC'GCT

Cuts at:      0    1054    3876 
   Size:       1054    2822
 
AhdI GACnn_n'nnGTC

Cuts at:      0     195    3876 
   Size:        195    3681
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     589    3876 
   Size:        589    3287
 
AlwNI CAG_nnn'CTG

Cuts at:      0     174     834    1801    2496    3293    3471    3621    3876 
   Size:        174     660     967     695     797     178     150     255

  Fragments arranged by size: 

                 967     797     695     660     255     178     174     150
            
 
ApaI G_GGCC'C

Cuts at:      0    2974    3876 
   Size:       2974     902
 
ApaLI G'TGCA_C

Cuts at:      0     220    1726    3861    3876 
   Size:        220    1506    2135      15

  Fragments arranged by size: 

                2135    1506     220      15
 
AvaI C'yCGr_G

Cuts at:      0     294     446     747    1220    1604    2543    2674    3358 
   Size:        294     152     301     473     384     939     131     684

Cuts at:   3358    3876 
   Size:        518

  Fragments arranged by size: 

                 939     684     518     473     384     301     294     152
                 131
 
AvrII C'CTAG_G

Cuts at:      0     996    3876 
   Size:        996    2880
 
BamHI G'GATC_C

Cuts at:      0       6    3876 
   Size:          6    3870
 
BanI G'GyrC_C

Cuts at:      0     102     108     377     441     535     679     709    3133 
   Size:        102       6     269      64      94     144      30    2424

Cuts at:   3133    3483    3610    3681    3876 
   Size:        350     127      71     195

  Fragments arranged by size: 

                2424     350     269     195     144     127     102      94
                  71      64      30       6
 
BanII G_rGCy'C

Cuts at:      0     295    1141    1358    2007    2273    2506    2974    3512 
   Size:        295     846     217     649     266     233     468     538

Cuts at:   3512    3595    3876 
   Size:         83     281

  Fragments arranged by size: 

                 846     649     538     468     295     281     266     233
                 217      83
 
BbeI G_GCGC'C

Cuts at:      0     106     112     683     713    3876 
   Size:        106       6     571      30    3163

  Fragments arranged by size: 

                3163     571     106      30       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1059    1244    2602    2775    3876 
   Size:       1059     185    1358     173    1101

  Fragments arranged by size: 

                1358    1101    1059     185     173
 
BbvCI CC'TCA_GC

Cuts at:      0    1659    1870    3627    3876 
   Size:       1659     211    1757     249

  Fragments arranged by size: 

                1757    1659     249     211
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     480     514    1297    1331    3876 
   Size:        480      34     783      34    2545

  Fragments arranged by size: 

                2545     783     480      34      34
 
BclI T'GATC_A

Cuts at:      0    2324    3876 
   Size:       2324    1552
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67    2383    2679    3131    3876 
   Size:         67    2316     296     452     745

  Fragments arranged by size: 

                2316     745     452     296      67
 
BglII A'GATC_T

Cuts at:      0     812    3876 
   Size:        812    3064
 
BlpI GC'TnA_GC

Cuts at:      0     856    1034    3876 
   Size:        856     178    2842

  Fragments arranged by size: 

                2842     856     178
 
Bme1580I G_kGCm'C

Cuts at:      0     224     446     952    1730    2648    2974    3047    3136 
   Size:        224     222     506     778     918     326      73      89

Cuts at:   3136    3488    3615    3865    3876 
   Size:        352     127     250      11

  Fragments arranged by size: 

                 918     778     506     352     326     250     224     222
                 127      89      73      11
 
BmgBI CAC'GTC

Cuts at:      0     738    1668    1890    3876 
   Size:        738     930     222    1986

  Fragments arranged by size: 

                1986     930     738     222
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1206    3683    3876 
   Size:       1206    2477     193

  Fragments arranged by size: 

                2477    1206     193
 
BmtI G_CTAG'C

Cuts at:      0    3635    3876 
   Size:       3635     241
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2802    3876 
   Size:       2802    1074
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23      41     804    1778    2308    2482    2815    2828 
   Size:         23      18     763     974     530     174     333      13

Cuts at:   2828    3169    3475    3876 
   Size:        341     306     401

  Fragments arranged by size: 

                 974     763     530     401     341     333     306     174
                  23      18      13
 
Bpu10I CC'TnA_GC

Cuts at:      0    1659    1870    3627    3876 
   Size:       1659     211    1757     249

  Fragments arranged by size: 

                1757    1659     249     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2455    2575    3876 
   Size:       2455     120    1301

  Fragments arranged by size: 

                2455    1301     120
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     403     757    3655    3876 
   Size:        403     354    2898     221

  Fragments arranged by size: 

                2898     403     354     221
 
BsaAI yAC'GTr

Cuts at:      0     363    3876 
   Size:        363    3513
 
BsaHI Gr'CG_yC

Cuts at:      0     103     109     389     503     680     710     725    1303 
   Size:        103       6     280     114     177      30      15     578

Cuts at:   1303    2054    2753    3876 
   Size:        751     699    1123

  Fragments arranged by size: 

                1123     751     699     578     280     177     114     103
                  30      15       6
 
BsaWI w'CCGG_w

Cuts at:      0    3722    3876 
   Size:       3722     154
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     112     142     199     229    1690    1720    1751    1781 
   Size:        112      30      57      30    1461      30      31      30

Cuts at:   1781    2062    2092    2539    2569    3876 
   Size:        281      30     447      30    1307

  Fragments arranged by size: 

                1461    1307     447     281     112      57      31      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     292     343    1312    1366    1476    1997    2158 
   Size:        289       3      51     969      54     110     521     161

Cuts at:   2158    2281    2638    2641    3876 
   Size:        123     357       3    1235

  Fragments arranged by size: 

                1235     969     521     357     289     161     123     110
                  54      51       3       3
 
BseYI C'CCAG_C

Cuts at:      0      81     627    1071    2111    2219    2707    3036    3338 
   Size:         81     546     444    1040     108     488     329     302

Cuts at:   3338    3513    3876 
   Size:        175     363

  Fragments arranged by size: 

                1040     546     488     444     363     329     302     175
                 108      81
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916    1367    1402    3346    3876 
   Size:        916     451      35    1944     530

  Fragments arranged by size: 

                1944     916     530     451      35
 
BsiEI CG_ry'CG

Cuts at:      0    1842    3876 
   Size:       1842    2034
 
BsiHKAI G_wGCw'C

Cuts at:      0     224     295     304     691     797     857    1730    2255 
   Size:        224      71       9     387     106      60     873     525

Cuts at:   2255    3194    3865    3876 
   Size:        939     671      11

  Fragments arranged by size: 

                 939     873     671     525     387     224     106      71
                  60      11       9
 
BsmI GAATG_Cn'

Cuts at:      0    1699    3876 
   Size:       1699    2177
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1018    1294    2218    3876 
   Size:       1018     276     924    1658

  Fragments arranged by size: 

                1658    1018     924     276
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     295     304     446     691     797     857     952 
   Size:        224      71       9     142     245     106      60      95

Cuts at:    952    1141    1358    1730    2007    2255    2273    2506    2648 
   Size:        189     217     372     277     248      18     233     142

Cuts at:   2648    2974    3047    3136    3194    3488    3512    3595    3615 
   Size:        326      73      89      58     294      24      83      20

Cuts at:   3615    3865    3876 
   Size:        250      11

  Fragments arranged by size: 

                 372     326     294     277     250     248     245     233
                 224     217     189     142     142     106      95      89
                  83      73      71      60      58      24      20      18
                  11       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     581     888    1167    1855    3876 
   Size:        581     307     279     688    2021

  Fragments arranged by size: 

                2021     688     581     307     279
 
BsrBI CCG'CTC

Cuts at:      0      37    1517    1706    3876 
   Size:         37    1480     189    2170

  Fragments arranged by size: 

                2170    1480     189      37
 
BsrDI GCAATG_nn'

Cuts at:      0     608    1931    3876 
   Size:        608    1323    1945

  Fragments arranged by size: 

                1945    1323     608
 
BsrFI r'CCGG_y

Cuts at:      0     105     160    2295    3244    3876 
   Size:        105      55    2135     949     632

  Fragments arranged by size: 

                2135     949     632     105      55
 
BsrGI T'GTAC_A

Cuts at:      0    2120    3876 
   Size:       2120    1756
 
BssSI C'ACGA_G

Cuts at:      0     325    1360    1527    3876 
   Size:        325    1035     167    2349

  Fragments arranged by size: 

                2349    1035     325     167
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     174     542    1182    3876 
   Size:        174     368     640    2694

  Fragments arranged by size: 

                2694     640     368     174
 
BstEII G'GTnAC_C

Cuts at:      0    1479    1644    3876 
   Size:       1479     165    2232

  Fragments arranged by size: 

                2232    1479     165
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1326    2737    3076    3876 
   Size:       1326    1411     339     800

  Fragments arranged by size: 

                1411    1326     800     339
 
BstYI r'GATC_y

Cuts at:      0       6     812    2456    3876 
   Size:          6     806    1644    1420

  Fragments arranged by size: 

                1644    1420     806       6
 
Bsu36I CC'TnA_GG

Cuts at:      0    1295    2306    2909    3554    3876 
   Size:       1295    1011     603     645     322

  Fragments arranged by size: 

                1295    1011     645     603     322
 
BtgI C'CryG_G

Cuts at:      0     210     246     285     354    1232    1688    2369    2381 
   Size:        210      36      39      69     878     456     681      12

Cuts at:   2381    3876 
   Size:       1495

  Fragments arranged by size: 

                1495     878     681     456     210      69      39      36
                  12
 
BtsI GCAGTG_nn'

Cuts at:      0    1640    2648    2740    2902    3081    3469    3876 
   Size:       1640    1008      92     162     179     388     407

  Fragments arranged by size: 

                1640    1008     407     388     179     162      92
 
DraIII CAC_nnn'GTG

Cuts at:      0    1214    3691    3876 
   Size:       1214    2477     185

  Fragments arranged by size: 

                2477    1214     185
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     520    1804    3171    3876 
   Size:        520    1284    1367     705

  Fragments arranged by size: 

                1367    1284     705     520
 
EaeI y'GGCC_r

Cuts at:      0    1145    1839    2366    2384    2612    3078    3540    3876 
   Size:       1145     694     527      18     228     466     462     336

  Fragments arranged by size: 

                1145     694     527     466     462     336     228      18
            
 
EagI C'GGCC_G

Cuts at:      0    1839    3876 
   Size:       1839    2037
 
EarI CTCTTCn'nnn_

Cuts at:      0     760     884    1723    1901    2327    2956    3736    3778 
   Size:        760     124     839     178     426     629     780      42

Cuts at:   3778    3876 
   Size:         98

  Fragments arranged by size: 

                 839     780     760     629     426     178     124      98
                  42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    3876 
   Size:         19    3857
 
EcoICRI GAG'CTC

Cuts at:      0     293    3876 
   Size:        293    3583
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23      41     685     804    1778    2308    2482    2815 
   Size:         23      18     644     119     974     530     174     333

Cuts at:   2815    2828    2847    3169    3475    3876 
   Size:         13      19     322     306     401

  Fragments arranged by size: 

                 974     644     530     401     333     322     306     174
                 119      23      19      18      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3184    3841    3876 
   Size:       3184     657      35

  Fragments arranged by size: 

                3184     657      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48     570    2970    2971    3876 
   Size:         48     522    2400       1     905

  Fragments arranged by size: 

                2400     905     522      48       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2877    3876 
   Size:       2877     999
 
HaeII r_GCGC'y

Cuts at:      0     106     112     174     683     713    1056    1164    2771 
   Size:        106       6      62     509      30     343     108    1607

Cuts at:   2771    2890    3313    3876 
   Size:        119     423     563

  Fragments arranged by size: 

                1607     563     509     423     343     119     108     106
                  62      30       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     181     213     247     279     440     472 
   Size:        112      32      37      32      34      32     161      32

Cuts at:    472     924     956    2011    2043    2309    2341    2668    2700 
   Size:        452      32    1055      32     266      32     327      32

Cuts at:   2700    2938    2970    3876 
   Size:        238      32     906

  Fragments arranged by size: 

                1055     906     452     327     266     238     161     112
                  37      34      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     507     561    3876 
   Size:        507      54    3315

  Fragments arranged by size: 

                3315     507      54
 
KasI G'GCGC_C

Cuts at:      0     102     108     679     709    3876 
   Size:        102       6     571      30    3167

  Fragments arranged by size: 

                3167     571     102      30       6
 
KpnI G_GTAC'C

Cuts at:      0     381    3876 
   Size:        381    3495
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     698    1733    2542    3876 
   Size:        698    1035     809    1334

  Fragments arranged by size: 

                1334    1035     809     698
 
MscI TGG'CCA

Cuts at:      0    1147    2368    2614    3876 
   Size:       1147    1221     246    1262

  Fragments arranged by size: 

                1262    1221    1147     246
 
MslI CAynn'nnrTG

Cuts at:      0    2482    3876 
   Size:       2482    1394
 
MspA1I CmG'CkG

Cuts at:      0     287     356    1038    1377    1523    2180    2219    2347 
   Size:        287      69     682     339     146     657      39     128

Cuts at:   2347    2493    3342    3517    3876 
   Size:        146     849     175     359

  Fragments arranged by size: 

                 849     682     657     359     339     287     175     146
                 146     128      69      39
 
NaeI GCC'GGC

Cuts at:      0     107     162    3246    3876 
   Size:        107      55    3084     630

  Fragments arranged by size: 

                3084     630     107      55
 
NarI GG'CG_CC

Cuts at:      0     103     109     680     710    3876 
   Size:        103       6     571      30    3166

  Fragments arranged by size: 

                3166     571     103      30       6
 
NcoI C'CATG_G

Cuts at:      0    1232    2369    2381    3876 
   Size:       1232    1137      12    1495

  Fragments arranged by size: 

                1495    1232    1137      12
 
NgoMIV G'CCGG_C

Cuts at:      0     105     160    3244    3876 
   Size:        105      55    3084     632

  Fragments arranged by size: 

                3084     632     105      55
 
NheI G'CTAG_C

Cuts at:      0    3631    3876 
   Size:       3631     245
 
NspI r_CATG'y

Cuts at:      0     403     468    3855    3876 
   Size:        403      65    3387      21

  Fragments arranged by size: 

                3387     403      65      21
 
PasI CC'CwG_GG

Cuts at:      0    1128    3588    3876 
   Size:       1128    2460     288

  Fragments arranged by size: 

                2460    1128     288
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1233    3621    3876 
   Size:       1233    2388     255

  Fragments arranged by size: 

                2388    1233     255
 
PfoI T'CCnGG_A

Cuts at:      0    2432    2459    3876 
   Size:       2432      27    1417

  Fragments arranged by size: 

                2432    1417      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     570    3876 
   Size:        570    3306
 
PshAI GACnn'nnGTC

Cuts at:      0     749    3876 
   Size:        749    3127
 
PspOMI G'GGCC_C

Cuts at:      0    2970    3876 
   Size:       2970     906
 
PstI C_TGCA'G

Cuts at:      0     578    3272    3876 
   Size:        578    2694     604

  Fragments arranged by size: 

                2694     604     578
 
PvuII CAG'CTG

Cuts at:      0    1377    2219    2347    2493    3342    3517    3876 
   Size:       1377     842     128     146     849     175     359

  Fragments arranged by size: 

                1377     849     842     359     175     146     128
 
SacI G_AGCT'C

Cuts at:      0     295    3876 
   Size:        295    3581
 
SacII CC_GC'GG

Cuts at:      0     288     357    3876 
   Size:        288      69    3519

  Fragments arranged by size: 

                3519     288      69
 
SbfI CC_TGCA'GG

Cuts at:      0    3272    3876 
   Size:       3272     604
 
SexAI A'CCwGG_T

Cuts at:      0     191    3876 
   Size:        191    3685
 
SfcI C'TryA_G

Cuts at:      0     574    2578    3268    3518    3876 
   Size:        574    2004     690     250     358

  Fragments arranged by size: 

                2004     690     574     358     250
 
SfoI GGC'GCC

Cuts at:      0     104     110     681     711    3876 
   Size:        104       6     571      30    3165

  Fragments arranged by size: 

                3165     571     104      30       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0     105    3876 
   Size:        105    3771
 
SmaI CCC'GGG

Cuts at:      0    1222    3876 
   Size:       1222    2654
 
SmlI C'TyrA_G

Cuts at:      0    1604    2470    2590    3876 
   Size:       1604     866     120    1286

  Fragments arranged by size: 

                1604    1286     866     120
 
SphI G_CATG'C

Cuts at:      0    3855    3876 
   Size:       3855      21
 
StuI AGG'CCT

Cuts at:      0    3266    3583    3876 
   Size:       3266     317     293

  Fragments arranged by size: 

                3266     317     293
 
StyI C'CwwG_G

Cuts at:      0     996    1076    1232    2369    2381    2771    3204    3488 
   Size:        996      80     156    1137      12     390     433     284

Cuts at:   3488    3785    3876 
   Size:        297      91

  Fragments arranged by size: 

                1137     996     433     390     297     284     156      91
                  80      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1000    1225    2436    3275    3702    3876 
   Size:       1000     225    1211     839     427     174

  Fragments arranged by size: 

                1211    1000     839     427     225     174
 
TatI w'GTAC_w

Cuts at:      0    2120    3876 
   Size:       2120    1756
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     454     486    1751    1783    2062    2094    3102    3134 
   Size:        454      32    1265      32     279      32    1008      32

Cuts at:   3134    3876 
   Size:        742

  Fragments arranged by size: 

                1265    1008     742     454     279      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     958    1972    3876 
   Size:        958    1014    1904

  Fragments arranged by size: 

                1904    1014     958
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     796    2283    2457    3339    3876 
   Size:        796    1487     174     882     537

  Fragments arranged by size: 

                1487     882     796     537     174
 
XhoI C'TCGA_G

Cuts at:      0    1604    3876 
   Size:       1604    2272
 
XmaI C'CCGG_G

Cuts at:      0    1220    3876 
   Size:       1220    2656
 
XmnI GAAnn'nnTTC

Cuts at:      0    1861    2860    3876 
   Size:       1861     999    1016

  Fragments arranged by size: 

                1861    1016     999
 
ZraI GAC'GTC

Cuts at:      0     504     726    2055    3876 
   Size:        504     222    1329    1821

  Fragments arranged by size: 

                1821    1329     504     222
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI     AhdI     AlfI    AlwNI
     ApaI    ApaLI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NspI     PasI    PflMI     PfoI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI    SacII     SbfI    SexAI     SfcI
     SfoI    SgrAI     SmaI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII   AflIII     AgeI     AleI     AloI     ApoI
     AscI     AseI    AsiSI     BaeI    BciVI    BfrBI    BsaBI    BsiWI
    BspEI    BspHI   BssHII    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     MfeI     MluI     NdeI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII
     SalI    SanDI     SapI     ScaI     SfiI     SgfI    SnaBI     SpeI
     SrfI     SspI     SwaI     XbaI
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