Restriction map for pF1KSDA1073
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AflIII      AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BanI        BbeI        BbrPI       
BbvCI       BclI        BglII       BlnI        BsaAI       
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BsmFI       BsmI        Bsp1407I    BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CpoI        DraI        
DrdI        EarI        Eco47III    EcoNI       EcoO65I     
FauI        FbaI        FseI        FspI        HgaI        
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MfeI        MluI        MroI        
MunI        MvnI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       Sse8387I    SspBI       StuI        
SwaI        XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1230  GACGTC               GACGT^C
AcyI         1230  GACGTC               G[GA]^CG[CT]C
AflII        1096  CTTAAG               C^TTAAG
AhdI           67  GACTCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         122  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1771  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI          67  GACTCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           12  CTCGAG               C^[CT]CG[GA]G
BamHI         357  GGATCC               G^GATCC
BcgI         1420  GCAGACAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          962  CCCGG                CC^[GC]GG
BfrI         1096  CTTAAG               C^TTAAG
BglI          985  GCCAACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1273  GCTCAGC              GC^T[ACGT]AGC
BpmI          672  CTGGAG               CTGGAG(16/14)
Bpu1102I     1273  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1021  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1230  GACGTC               G[GA]^CG[CT]C
BsgI         1895  CTGCAC               GTGCAG(16/14)
BsiEI          52  CGGCCG               CG[GA][CT]^CG
BsmBI           4  GAGACG               CGTCTC(1/5)
BsoBI          12  CTCGAG               C^[CT]CG[GA]G
BspDI        1373  ATCGAT               AT^CGAT
BspMI         566  ACCTGC               ACCTGC(4/8)
BsrDI        1246  CATTGC               GCAATG(2/0)
BsrFI          39  GCCGGC               [GA]^CCGG[CT]
CelII        1273  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         39  GCCGGC               [GA]^CCGG[CT]
ClaI         1373  ATCGAT               AT^CGAT
DraII         185  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EagI           52  CGGCCG               C^GGCCG
Eam1105I       67  GACTCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          52  CGGCCG               C^GGCCG
Eco52I         52  CGGCCG               C^GGCCG
EcoO109I      185  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1054  GATATC               GAT^ATC
EcoT22I       545  ATGCAT               ATGCA^T
HaeII         295  GGCGCT               [GA]GCGC^[CT]
Hin1I        1230  GACGTC               G[GA]^CG[CT]C
HindIII      1177  AAGCTT               A^AGCTT
HphI          433  GGTGA                GGTGA(8/7)
MaeII        1231  ACGT                 A^CGT
MamI         1021  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI           39  GCCGGC               GCC^GGC
NciI          962  CCCGG                CC^[GC]GG
NgoMIV         39  GCCGGC               G^CCGGC
NsiI          545  ATGCAT               ATGCA^T
NspI         1706  GCATGC               [GA]CATG^[CT]
PaeR7I         12  CTCGAG               C^TCGAG
PpuMI         185  GGGTCCT              [GA]G^G[AT]CC[CT]
SfcI         1840  CTACAG               C^T[GA][CT]AG
SpeI         1041  ACTAGT               A^CTAGT
SphI         1706  GCATGC               GCATG^C
XhoI           12  CTCGAG               C^TCGAG
XmnI         1771  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AfaI          301  GTAC                 GT^AC
             1110  GTAC                
AocI          189  CCTGAGG              CC^T[ACGT]AGG
              499  CCTGAGG             
AspHI           9  GAGCTC               G[AT]GC[AT]^C
             1720  GAGCTC              
AspI         1453  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1608  GACTGTGTC           
AvaII         186  GGTCC                G^G[AT]CC
             1527  GGACC               
BalI          260  TGGCCA               TGG^CCA
             1392  TGGCCA              
BbsI         1227  GAAGAC               GAAGAC(2/6)
             1645  GAAGAC              
BbvI           17  GCTGC                GCAGC(8/12)
             1541  GCTGC               
BciVI        1309  GGATAC               GTATCC(6/5)
              797  GTATCC              
BpuAI        1227  GAAGAC               GAAGAC(2/6)
             1645  GAAGAC              
BsiHKAI         9  GAGCTC               G[AT]GC[AT]^C
             1720  GAGCTC              
BstYI         357  GGATCC               [GA]^GATC[CT]
              517  AGATCC              
Bsu36I        189  CCTGAGG              CC^T[ACGT]AGG
              499  CCTGAGG             
CfoI          296  GCGC                 GCG^C
             1710  GCGC                
Cfr13I        186  GGTCC                G^G[ACGT]CC
             1527  GGACC               
DraIII        843  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1898  CACAGTGTG           
Eco81I        189  CCTGAGG              CC^T[ACGT]AGG
              499  CCTGAGG             
HapII          40  CCGG                 C^CGG
              963  CCGG                
HhaI          296  GCGC                 GCG^C
             1710  GCGC                
HinP1I        296  GCGC                 G^CGC
             1710  GCGC                
HpaII          40  CCGG                 C^CGG
              963  CCGG                
MflI          357  GGATCC               [GA]^GATC[CT]
              517  AGATCC              
MluNI         260  TGGCCA               TGG^CCA
             1392  TGGCCA              
MscI          260  TGGCCA               TGG^CCA
             1392  TGGCCA              
MspA1I       1539  CAGCTG               C[AC]G^C[GT]G
             1639  CAGCTG              
MspI           40  CCGG                 C^CGG
              963  CCGG                
NcoI          860  CCATGG               C^CATGG
             1395  CCATGG              
PflFI        1453  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1608  GACTGTGTC           
PflMI         286  CCATTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              855  CCAGCCCATGG         
PvuII        1539  CAGCTG               CAG^CTG
             1639  CAGCTG              
RsaI          301  GTAC                 GT^AC
             1110  GTAC                
SacI            9  GAGCTC               GAGCT^C
             1720  GAGCTC              
Sau96I        186  GGTCC                G^G[ACGT]CC
             1527  GGACC               
SmlI           12  CTCGAG               C^T[CT][GA]AG
             1096  CTTAAG              
TseI           17  GCTGC                G^C[AT]GC
             1541  GCTGC               
Tth111I      1453  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1608  GACTGTGTC           
Van91I        286  CCATTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              855  CCAGCCCATGG         
XhoII         357  GGATCC               [GA]^GATC[CT]
              517  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
BanII       BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       DdeI        DpnII       
DsaI        EaeI        EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        Sau3AI      ScrFI       
SfaNI       SspI        StyI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        
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