Restriction map for pF1KSDA0945
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AvrII       BciVI       BclI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HgaI        HincII      HindII      HindIII     
HpaI        Ksp632I     KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NotI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1770  GGTACC               G^GTACC
AccII        2075  CGCG                 CG^CG
AcsI         1456  GAATTT               [GA]^AATT[CT]
AflII        2323  CTTAAG               C^TTAAG
AflIII       1716  ACGTGT               A^C[GA][CT]GT
ApoI         1456  GAATTT               [GA]^AATT[CT]
Asp718I      1770  GGTACC               G^GTACC
AspI         1885  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1643  TGCGCA               TGC^GCA
BamHI         447  GGATCC               G^GATCC
BbrPI        1715  CACGTG               CAC^GTG
BbvCI        1048  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1256  CGAGCTCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2323  CTTAAG               C^TTAAG
BglII        1940  AGATCT               A^GATCT
BsaAI        1715  CACGTG               [CT]AC^GT[GA]
BsaI          590  GAGACC               GGTCTC(1/5)
BsrFI         241  GCCGGT               [GA]^CCGG[CT]
BstAPI       1017  GCAGGAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        136  GGTCACC              G^GT[ACGT]ACC
BstPI         136  GGTCACC              G^GT[ACGT]ACC
BstUI        2075  CGCG                 CG^CG
Cfr10I        241  GCCGGT               [GA]^CCGG[CT]
EcoNI         897  CCTGAGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       136  GGTCACC              G^GT[ACGT]ACC
FspI         1643  TGCGCA               TGC^GCA
KpnI         1770  GGTACC               GGTAC^C
MaeII        1716  ACGT                 A^CGT
MseI         2324  TTAA                 T^TAA
MvnI         2075  CGCG                 CG^CG
NheI          613  GCTAGC               G^CTAGC
NruI         2074  TCGCGA               TCG^CGA
PflFI        1885  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         773  CCAAGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1715  CACGTG               CAC^GTG
PmlI         1715  CACGTG               CAC^GTG
ScaI         2141  AGTACT               AGT^ACT
SmlI         2323  CTTAAG               C^T[CT][GA]AG
TaqI          438  TCGA                 T^CGA
Tru9I        2324  TTAA                 T^TAA
Tth111I      1885  GACACCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       438  TCGA                 T^CGA
Van91I        773  CCAAGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1922  CCACACCTTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AocI         1198  CCTGAGG              CC^T[ACGT]AGG
             1268  CCTCAGG             
BbsI           76  GAAGAC               GAAGAC(2/6)
              319  GAAGAC              
BpuAI          76  GAAGAC               GAAGAC(2/6)
              319  GAAGAC              
BsrBI         652  GAGCGG               CCGCTC(-3/-3)
             1855  GAGCGG              
Bsu36I       1198  CCTGAGG              CC^T[ACGT]AGG
             1268  CCTCAGG             
Eco81I       1198  CCTGAGG              CC^T[ACGT]AGG
             1268  CCTCAGG             
MslI         1329  CACTGGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2293  CATGTCTATG          
NcoI          844  CCATGG               C^CATGG
             2159  CCATGG              
NspI          119  GCATGC               [GA]CATG^[CT]
             2039  GCATGC              
SacI         1114  GAGCTC               GAGCT^C
             1257  GAGCTC              
SfiI           43  GGCCTCACTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              737  GGCCAAAGTGGCC       
SphI          119  GCATGC               GCATG^C
             2039  GCATGC              
Tsp509I       160  AATT                 ^AATT
             1457  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MspA1I      MspI        MvaI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        StuI        StyI        TseI        Tsp45I      
TspRI       XhoII       
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