Restriction map for pF1KSDA0842
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AscI        
AseI        AsnI        Asp718I     AviII       AvrII       
BbeI        BfrI        BglII       BlnI        BsaBI       
BsiWI       Bsp1407I    BspDI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        DraI        EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MluI        MseI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        SalI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SspBI       SspI        
SwaI        Tru9I       XbaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        2434  GACGTC               GACGT^C
AccII        3028  CGCG                 CG^CG
AflIII       1796  ACGTGT               A^C[GA][CT]GT
AocI         1489  CCTGAGG              CC^T[ACGT]AGG
ApaI         2274  GGGCCC               GGGCC^C
BbvCI        1693  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1554  GGATAC               GTATCC(6/5)
BclI         2105  TGATCA               T^GATCA
BsmBI        2769  CGTCTC               CGTCTC(1/5)
BsmI          378  GAATGC               GAATGC(1/-1)
BspHI        1787  TCATGA               T^CATGA
BstEII        942  GGTCACC              G^GT[ACGT]ACC
BstPI         942  GGTCACC              G^GT[ACGT]ACC
BstUI        3028  CGCG                 CG^CG
Bsu36I       1489  CCTGAGG              CC^T[ACGT]AGG
DraIII       1272  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         3030  CGGCCG               C^GGCCG
EclXI        3030  CGGCCG               C^GGCCG
Eco52I       3030  CGGCCG               C^GGCCG
Eco81I       1489  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       942  GGTCACC              G^GT[ACGT]ACC
FbaI         2105  TGATCA               T^GATCA
KspI         3027  CCGCGG               CCGC^GG
MvnI         3028  CGCG                 CG^CG
NaeI          825  GCCGGC               GCC^GGC
NgoMIV        825  GCCGGC               G^CCGGC
PaeR7I        928  CTCGAG               C^TCGAG
RcaI         1787  TCATGA               T^CATGA
SacI          925  GAGCTC               GAGCT^C
SacII        3027  CCGCGG               CCGC^GG
SbfI         1450  CCTGCAGG             CCTGCA^GG
SfiI         2574  GGCCGAGATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1324  CCCGGG               CCC^GGG
SphI         2327  GCATGC               GCATG^C
Sse8387I     1450  CCTGCAGG             CCTGCA^GG
StuI          776  AGGCCT               AGG^CCT
XhoI          928  CTCGAG               C^TCGAG
XmaCI        1324  CCCGGG               C^CCGGG
XmaI         1324  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         1762  GTAGAC               GT^[AC][GT]AC
             1903  GTCTAC              
AcsI         2179  AAATTT               [GA]^AATT[CT]
             2968  AAATTC              
AcyI         1536  GACGCC               G[GA]^CG[CT]C
             2434  GACGTC              
AhdI          568  GACAGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1975  GACTATGTGTC         
ApoI         2179  AAATTT               [GA]^AATT[CT]
             2968  AAATTC              
Asp700I       807  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2964  GAAGAAATTC          
AspEI         568  GACAGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1975  GACTATGTGTC         
BamHI          26  GGATCC               G^GATCC
             2405  GGATCC              
BanI         2308  GGCACC               G^G[CT][GA]CC
             2347  GGCACC              
BbrPI         253  CACGTG               CAC^GTG
             1795  CACGTG              
BbsI          535  GAAGAC               GAAGAC(2/6)
             2376  GAAGAC              
BglI         1058  GCCGCAACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2575  GCCGAGATGGC         
BlpI         1288  GCTGAGC              GC^T[ACGT]AGC
             2394  GCTCAGC             
Bpu1102I     1288  GCTGAGC              GC^T[ACGT]AGC
             2394  GCTCAGC             
BpuAI         535  GAAGAC               GAAGAC(2/6)
             2376  GAAGAC              
BsaHI        1536  GACGCC               G[GA]^CG[CT]C
             2434  GACGTC              
BsiEI        2538  CGACCG               CG[GA][CT]^CG
             3030  CGGCCG              
BsrBI         744  CCGCTC               CCGCTC(-3/-3)
             2730  CCGCTC              
BssSI        1560  CACGAG               CACGAG(-5/-1)
              199  CTCGTG              
CelII        1288  GCTGAGC              GC^T[ACGT]AGC
             2394  GCTCAGC             
CpoI         1312  CGGACCG              CG^G[AT]CCG
             2423  CGGACCG             
DrdI          420  GACCTTGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1894  GACTGCTATGTC        
Eam1105I      568  GACAGTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1975  GACTATGTGTC         
Hin1I        1536  GACGCC               G[GA]^CG[CT]C
             2434  GACGTC              
MslI          855  CACCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1200  CACCTCCATG          
NcoI         1205  CCATGG               C^CATGG
             2156  CCATGG              
PmaCI         253  CACGTG               CAC^GTG
             1795  CACGTG              
PmlI          253  CACGTG               CAC^GTG
             1795  CACGTG              
RsrII        1312  CGGACCG              CG^G[AT]CCG
             2423  CGGACCG             
SapI         1813  GAAGAGC              GCTCTTC(1/4)
              411  GCTCTTC             
ScaI          365  AGTACT               AGT^ACT
             2792  AGTACT              
SexAI        1273  ACCTGGT              A^CC[AT]GGT
             1940  ACCTGGT             
TfiI         1699  GATTC                G^A[AT]TC
             2194  GAATC               
XmnI          807  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2964  GAAGAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccIII      AciI        AfaI        AluI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaLI       AspHI       
AspI        AvaI        AvaII       BalI        BanII       
BbvI        BcgI        BcnI        BfaI        BmyI        
BpmI        BsaAI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspEI       BspMI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com