Restriction map for pF1KSDA0742
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AcyI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsiEI       BsiWI       BsmBI       
BspDI       BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     CelII       
ClaI        CpoI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoT22I     
FseI        FspI        HaeII       Hin1I       HpaI        
KasI        KpnI        KspI        MamI        MluI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII         109  CGCG                 CG^CG
AccIII       2654  TCCGGA               T^CCGGA
AclI         1825  AACGTT               AA^CGTT
AflIII        748  ACATGT               A^C[GA][CT]GT
AlwNI        2384  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1240  CCTAAGG              CC^T[ACGT]AGG
ApaI         3288  GGGCCC               GGGCC^C
AseI         3315  ATTAAT               AT^TAAT
AsnI         3315  ATTAAT               AT^TAAT
Asp700I      3082  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         3214  CCCGAG               C^[CT]CG[GA]G
BamHI         376  GGATCC               G^GATCC
BglII        3282  AGATCT               A^GATCT
BsaI         1877  GAGACC               GGTCTC(1/5)
BseAI        2654  TCCGGA               T^CCGGA
BsoBI        3214  CCCGAG               C^[CT]CG[GA]G
BspEI        2654  TCCGGA               T^CCGGA
BspHI        3621  TCATGA               T^CATGA
BspLU11I      748  ACATGT               A^CATGT
BstEII       1808  GGTTACC              G^GT[ACGT]ACC
BstPI        1808  GGTTACC              G^GT[ACGT]ACC
BstUI         109  CGCG                 CG^CG
BstXI        2801  CCAGCATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1240  CCTAAGG              CC^T[ACGT]AGG
CfoI          108  GCGC                 GCG^C
DraIII       2017  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1240  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1236  CCTTCCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1808  GGTTACC              G^GT[ACGT]ACC
EcoRV        3842  GATATC               GAT^ATC
EcoT14I       984  CCTTGG               C^C[AT][AT]GG
HgaI          110  GCGTC                GACGC(5/10)
HhaI          108  GCGC                 GCG^C
HinP1I        108  GCGC                 G^CGC
HindIII       512  AAGCTT               A^AGCTT
MfeI         1796  CAATTG               C^AATTG
MroI         2654  TCCGGA               T^CCGGA
MunI         1796  CAATTG               C^AATTG
MvnI          109  CGCG                 CG^CG
NaeI         2419  GCCGGC               GCC^GGC
NgoMIV       2419  GCCGGC               G^CCGGC
PpuMI         935  AGGACCC              [GA]G^G[AT]CC[CT]
PshBI        3315  ATTAAT               AT^TAAT
Psp1406I     1825  AACGTT               AA^CGTT
RcaI         3621  TCATGA               T^CATGA
SacI         3737  GAGCTC               GAGCT^C
SapI         3580  GAAGAGC              GCTCTTC(1/4)
ScaI          952  AGTACT               AGT^ACT
StyI          984  CCTTGG               C^C[AT][AT]GG
XmnI         3082  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          238  GTCTAC               GT^[AC][GT]AC
             3010  GTAGAC              
AflII        3133  CTTAAG               C^TTAAG
             3439  CTTAAG              
BanII        3288  GGGCCC               G[GA]GC[CT]^C
             3737  GAGCTC              
BclI         1191  TGATCA               T^GATCA
             3624  TGATCA              
BcnI          134  CCGGG                CC^[GC]GG
             3729  CCCGG               
BfrI         3133  CTTAAG               C^TTAAG
             3439  CTTAAG              
BsaWI        2063  ACCGGA               [AT]^CCGG[AT]
             2654  TCCGGA              
BsmFI          98  GGGAC                GGGAC(10/14)
              136  GGGAC               
BsmI         1515  GAATGC               GAATGC(1/-1)
             2804  GCATTC              
BspMI        2012  ACCTGC               ACCTGC(4/8)
             1369  GCAGGT              
BsrFI          27  GCCGGT               [GA]^CCGG[CT]
             2419  GCCGGC              
Cfr10I         27  GCCGGT               [GA]^CCGG[CT]
             2419  GCCGGC              
DraII         935  AGGACCC              [GA]G^G[ACGT]CC[CT]
             3288  GGGCCCC             
EcoO109I      935  AGGACCC              [GA]G^G[ACGT]CC[CT]
             3288  GGGCCCC             
FauI         1752  GCGGG                CCCGC(4/6)
             2486  GCGGG               
FbaI         1191  TGATCA               T^GATCA
             3624  TGATCA              
NciI          134  CCGGG                CC^[GC]GG
             3729  CCCGG               
PstI          914  CTGCAG               CTGCA^G
             1306  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BalI        BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EaeI        EarI        EcoRI       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       StuI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
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