Restriction map for
pF1KSDA0647
(Linear) (Six-base) MAPSORT of: KIAA0647.seq Check: 4929 from: 1 to: 3585
KIAA0647.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 471 1187 3120 3585
Size: 471 716 1933 465
Fragments arranged by size:
1933 716 471 465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 392 3121 3469 3577 3585
Size: 73 319 2729 348 108 8
Fragments arranged by size:
2729 348 319 108 73 8
AflII C'TTAA_G
Cuts at: 0 1441 3585
Size: 1441 2144
AflIII A'CryG_T
Cuts at: 0 3211 3585
Size: 3211 374
AleI CACnn'nnGTG
Cuts at: 0 3055 3164 3585
Size: 3055 109 421
Fragments arranged by size:
3055 421 109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3372 3585
Size: 3372 213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 607 639 3585
Size: 607 32 2946
Fragments arranged by size:
2946 607 32
AlwNI CAG_nnn'CTG
Cuts at: 0 576 2278 2518 3585
Size: 576 1702 240 1067
Fragments arranged by size:
1702 1067 576 240
ApaI G_GGCC'C
Cuts at: 0 17 1686 2840 3585
Size: 17 1669 1154 745
Fragments arranged by size:
1669 1154 745 17
ApaLI G'TGCA_C
Cuts at: 0 2285 3585
Size: 2285 1300
ApoI r'AATT_y
Cuts at: 0 187 1465 3376 3585
Size: 187 1278 1911 209
Fragments arranged by size:
1911 1278 209 187
AvaI C'yCGr_G
Cuts at: 0 786 2129 3585
Size: 786 1343 1456
Fragments arranged by size:
1456 1343 786
BamHI G'GATC_C
Cuts at: 0 1092 3585
Size: 1092 2493
BanI G'GyrC_C
Cuts at: 0 293 404 1522 3585
Size: 293 111 1118 2063
Fragments arranged by size:
2063 1118 293 111
BanII G_rGCy'C
Cuts at: 0 17 811 1686 1937 2840 2862 3185 3585
Size: 17 794 875 251 903 22 323 400
Fragments arranged by size:
903 875 794 400 323 251 22 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 380 2014 2333 2588 3275 3402 3585
Size: 380 1634 319 255 687 127 183
Fragments arranged by size:
1634 687 380 319 255 183 127
BbvCI CC'TCA_GC
Cuts at: 0 814 1997 2431 3585
Size: 814 1183 434 1154
Fragments arranged by size:
1183 1154 814 434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3100 3134 3585
Size: 3100 34 451
Fragments arranged by size:
3100 451 34
BclI T'GATC_A
Cuts at: 0 2421 3258 3465 3585
Size: 2421 837 207 120
Fragments arranged by size:
2421 837 207 120
BglI GCCn_nnn'nGGC
Cuts at: 0 961 1520 3585
Size: 961 559 2065
Fragments arranged by size:
2065 961 559
BlpI GC'TnA_GC
Cuts at: 0 340 3585
Size: 340 3245
Bme1580I G_kGCm'C
Cuts at: 0 17 559 780 1343 1686 2289 2405 2840
Size: 17 542 221 563 343 603 116 435
Cuts at: 2840 3171 3585
Size: 331 414
Fragments arranged by size:
603 563 542 435 414 343 331 221
116 17
BmgBI CAC'GTC
Cuts at: 0 507 758 3585
Size: 507 251 2827
Fragments arranged by size:
2827 507 251
BmrI ACTGGGnnnn_n'
Cuts at: 0 554 812 1762 2727 3478 3585
Size: 554 258 950 965 751 107
Fragments arranged by size:
965 950 751 554 258 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 3585
Size: 348 3237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 540 631 905 1138 1396 1951 2180
Size: 46 494 91 274 233 258 555 229
Cuts at: 2180 2990 3158 3585
Size: 810 168 427
Fragments arranged by size:
810 555 494 427 274 258 233 229
168 91 46
Bpu10I CC'TnA_GC
Cuts at: 0 814 1997 2431 3585
Size: 814 1183 434 1154
Fragments arranged by size:
1183 1154 814 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2079 3585
Size: 2079 1506
BsaWI w'CCGG_w
Cuts at: 0 218 1046 2663 3585
Size: 218 828 1617 922
Fragments arranged by size:
1617 922 828 218
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 888 918 1492 1522 3585
Size: 888 30 574 30 2063
Fragments arranged by size:
2063 888 574 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 436 896 2018 2021 2462 3005 3585
Size: 22 414 460 1122 3 441 543 580
Fragments arranged by size:
1122 580 543 460 441 414 22 3
BseYI C'CCAG_C
Cuts at: 0 19 1229 1642 2038 2220 2378 3290 3329
Size: 19 1210 413 396 182 158 912 39
Cuts at: 3329 3585
Size: 256
Fragments arranged by size:
1210 912 413 396 256 182 158 39
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1672 3585
Size: 529 1143 1913
Fragments arranged by size:
1913 1143 529
BsiHKAI G_wGCw'C
Cuts at: 0 461 883 898 2289 3585
Size: 461 422 15 1391 1296
Fragments arranged by size:
1391 1296 461 422 15
BsmI GAATG_Cn'
Cuts at: 0 1477 3585
Size: 1477 2108
Bsp1286I G_dGCh'C
Cuts at: 0 17 461 559 780 811 883 898 1343
Size: 17 444 98 221 31 72 15 445
Cuts at: 1343 1686 1937 2289 2405 2840 2862 3171 3185
Size: 343 251 352 116 435 22 309 14
Cuts at: 3185 3585
Size: 400
Fragments arranged by size:
445 444 435 400 352 343 309 251
221 116 98 72 31 22 17 15
14
BspEI T'CCGG_A
Cuts at: 0 218 1046 2663 3585
Size: 218 828 1617 922
Fragments arranged by size:
1617 922 828 218
BspHI T'CATG_A
Cuts at: 0 3542 3585
Size: 3542 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 369 1588 3585
Size: 369 1219 1997
Fragments arranged by size:
1997 1219 369
BsrBI CCG'CTC
Cuts at: 0 632 1799 3585
Size: 632 1167 1786
Fragments arranged by size:
1786 1167 632
BsrDI GCAATG_nn'
Cuts at: 0 155 681 738 761 3402 3566 3585
Size: 155 526 57 23 2641 164 19
Fragments arranged by size:
2641 526 164 155 57 23 19
BsrFI r'CCGG_y
Cuts at: 0 173 1199 3585
Size: 173 1026 2386
Fragments arranged by size:
2386 1026 173
BssSI C'ACGA_G
Cuts at: 0 3527 3585
Size: 3527 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 148 361 2441 3585
Size: 148 213 2080 1144
Fragments arranged by size:
2080 1144 213 148
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1830 3585
Size: 1830 1755
BstYI r'GATC_y
Cuts at: 0 45 1092 1752 1825 2505 2562 3585
Size: 45 1047 660 73 680 57 1023
Fragments arranged by size:
1047 1023 680 660 73 57 45
Bsu36I CC'TnA_GG
Cuts at: 0 1952 2511 3585
Size: 1952 559 1074
Fragments arranged by size:
1952 1074 559
BtgI C'CryG_G
Cuts at: 0 650 1829 2571 3050 3188 3585
Size: 650 1179 742 479 138 397
Fragments arranged by size:
1179 742 650 479 397 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 672 3585
Size: 672 2913
BtsI GCAGTG_nn'
Cuts at: 0 322 956 1215 2129 3159 3240 3354 3585
Size: 322 634 259 914 1030 81 114 231
Fragments arranged by size:
1030 914 634 322 259 231 114 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1955 1990 3585
Size: 1955 35 1595
Fragments arranged by size:
1955 1595 35
DraIII CAC_nnn'GTG
Cuts at: 0 2455 3585
Size: 2455 1130
EaeI y'GGCC_r
Cuts at: 0 953 1028 1259 1362 1532 1794 3386 3585
Size: 953 75 231 103 170 262 1592 199
Fragments arranged by size:
1592 953 262 231 199 170 103 75
EarI CTCTTCn'nnn_
Cuts at: 0 74 983 2134 2171 3585
Size: 74 909 1151 37 1414
Fragments arranged by size:
1414 1151 909 74 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 3585
Size: 496 3089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 73 392 540 631 905 1138 1396
Size: 46 27 319 148 91 274 233 258
Cuts at: 1396 1951 2180 2990 3121 3158 3469 3577 3585
Size: 555 229 810 131 37 311 108 8
Fragments arranged by size:
810 555 319 311 274 258 233 229
148 131 108 91 46 37 27 8
EcoO109I rG'GnC_Cy
Cuts at: 0 13 14 93 1683 1993 2836 2837 3585
Size: 13 1 79 1590 310 843 1 748
Fragments arranged by size:
1590 843 748 310 79 13 1 1
EcoRI G'AATT_C
Cuts at: 0 3376 3585
Size: 3376 209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 103 2038 3292 3585
Size: 103 1935 1254 293
Fragments arranged by size:
1935 1254 293 103
FseI GG_CCGG'CC
Cuts at: 0 1203 3585
Size: 1203 2382
FspI TGC'GCA
Cuts at: 0 673 3585
Size: 673 2912
HaeII r_GCGC'y
Cuts at: 0 1597 3585
Size: 1597 1988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 919 951 1077 1109 3238 3270 3585
Size: 919 32 126 32 2129 32 315
Fragments arranged by size:
2129 919 315 126 32 32 32
HincII GTy'rAC
Cuts at: 0 472 3121 3585
Size: 472 2649 464
Fragments arranged by size:
2649 472 464
HindIII A'AGCT_T
Cuts at: 0 1968 3585
Size: 1968 1617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1274 2738 3585
Size: 1274 1464 847
Fragments arranged by size:
1464 1274 847
MscI TGG'CCA
Cuts at: 0 955 1030 1261 1364 1534 3388 3585
Size: 955 75 231 103 170 1854 197
Fragments arranged by size:
1854 955 231 197 170 103 75
MslI CAynn'nnrTG
Cuts at: 0 1024 1725 2903 3055 3164 3585
Size: 1024 701 1178 152 109 421
Fragments arranged by size:
1178 1024 701 421 152 109
MspA1I CmG'CkG
Cuts at: 0 695 716 1165 1702 3555 3585
Size: 695 21 449 537 1853 30
Fragments arranged by size:
1853 695 537 449 30 21
NaeI GCC'GGC
Cuts at: 0 1201 3585
Size: 1201 2384
NcoI C'CATG_G
Cuts at: 0 1829 2571 3188 3585
Size: 1829 742 617 397
Fragments arranged by size:
1829 742 617 397
NdeI CA'TA_TG
Cuts at: 0 3344 3585
Size: 3344 241
NgoMIV G'CCGG_C
Cuts at: 0 1199 3585
Size: 1199 2386
NspI r_CATG'y
Cuts at: 0 3215 3585
Size: 3215 370
PciI A'CATG_T
Cuts at: 0 3211 3585
Size: 3211 374
PflMI CCAn_nnn'nTGG
Cuts at: 0 2736 3585
Size: 2736 849
PfoI T'CCnGG_A
Cuts at: 0 634 923 3585
Size: 634 289 2662
Fragments arranged by size:
2662 634 289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 3585
Size: 1000 32 2553
Fragments arranged by size:
2553 1000 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 3585
Size: 93 3492
PshAI GACnn'nnGTC
Cuts at: 0 3155 3585
Size: 3155 430
PspOMI G'GGCC_C
Cuts at: 0 13 1682 2836 3585
Size: 13 1669 1154 749
Fragments arranged by size:
1669 1154 749 13
PstI C_TGCA'G
Cuts at: 0 113 700 1139 3585
Size: 113 587 439 2446
Fragments arranged by size:
2446 587 439 113
PvuII CAG'CTG
Cuts at: 0 716 1165 1702 3555 3585
Size: 716 449 537 1853 30
Fragments arranged by size:
1853 716 537 449 30
SalI G'TCGA_C
Cuts at: 0 470 3119 3585
Size: 470 2649 466
Fragments arranged by size:
2649 470 466
SapI GCTCTTCn'nnn_
Cuts at: 0 2134 3585
Size: 2134 1451
ScaI AGT'ACT
Cuts at: 0 994 3585
Size: 994 2591
SexAI A'CCwGG_T
Cuts at: 0 749 3585
Size: 749 2836
SfcI C'TryA_G
Cuts at: 0 109 696 1135 2006 2357 3572 3585
Size: 109 587 439 871 351 1215 13
Fragments arranged by size:
1215 871 587 439 351 109 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 961 3585
Size: 961 2624
SmaI CCC'GGG
Cuts at: 0 788 3585
Size: 788 2797
SmlI C'TyrA_G
Cuts at: 0 1441 2058 3585
Size: 1441 617 1527
Fragments arranged by size:
1527 1441 617
SpeI A'CTAG_T
Cuts at: 0 66 3585
Size: 66 3519
SspI AAT'ATT
Cuts at: 0 1268 3585
Size: 1268 2317
StuI AGG'CCT
Cuts at: 0 1928 3585
Size: 1928 1657
StyI C'CwwG_G
Cuts at: 0 41 59 965 1829 2294 2571 2730 3188
Size: 41 18 906 864 465 277 159 458
Cuts at: 3188 3585
Size: 397
Fragments arranged by size:
906 864 465 458 397 277 159 41
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 1656 3585
Size: 433 1223 1929
Fragments arranged by size:
1929 1223 433
TatI w'GTAC_w
Cuts at: 0 29 992 2914 3585
Size: 29 963 1922 671
Fragments arranged by size:
1922 963 671 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 888 920 1492 1524 2139 2171 3585
Size: 888 32 572 32 615 32 1414
Fragments arranged by size:
1414 888 615 572 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 199 1654 3585
Size: 199 1455 1931
Fragments arranged by size:
1931 1455 199
XbaI T'CTAG_A
Cuts at: 0 2475 3585
Size: 2475 1110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 713 1269 1650 1789 3585
Size: 713 556 381 139 1796
Fragments arranged by size:
1796 713 556 381 139
XmaI C'CCGG_G
Cuts at: 0 786 3585
Size: 786 2799
XmnI GAAnn'nnTTC
Cuts at: 0 3515 3585
Size: 3515 70
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EarI EciI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SalI SapI ScaI SexAI SfcI
SfiI SmaI SmlI SpeI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BglII
BmtI BsaI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI
BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI DrdI
EagI EcoICRI EcoNI EcoRV FspAI HpaI KasI KpnI
MfeI MluI NarI NheI NotI NruI NsiI PacI
PasI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI SbfI SfoI SgfI SgrAI SnaBI
SphI SrfI SwaI XhoI ZraI