Restriction map for pF1KSDA0642
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AccII       AclI        AflII       AflIII      AgeI        
Aor51HI     AscI        AseI        AsnI        AviII       
BalI        BamHI       BbrPI       BbvCI       BcgI        
BciVI       BfrI        BglII       BlpI        Bpu1102I    
BsaAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DsaI        
EagI        EclXI       Eco47III    Eco52I      FseI        
FspI        HincII      HindII      HpaI        KspI        
MfeI        MluI        MluNI       MscI        MunI        
MvnI        NaeI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII         818  GACGTC               GACGT^C
AccI         1313  GTCTAC               GT^[AC][GT]AC
AccIII       2780  TCCGGA               T^CCGGA
AhdI         1216  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1577  GTGCAC               G^TGCAC
AocI         1684  CCTCAGG              CC^T[ACGT]AGG
ApaI         2612  GGGCCC               GGGCC^C
ApaLI        1577  GTGCAC               G^TGCAC
AspEI        1216  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3241  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2615  CCCGGG               C^[CT]CG[GA]G
AvrII        3325  CCTAGG               C^CTAGG
BbeI         2957  GGCGCC               GGCGC^C
BclI         2076  TGATCA               T^GATCA
BglI           33  GCCTGAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3325  CCTAGG               C^CTAGG
BsaBI        1595  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2780  TCCGGA               [AT]^CCGG[AT]
BseAI        2780  TCCGGA               T^CCGGA
BsmBI        3943  GAGACG               CGTCTC(1/5)
BsoBI        2615  CCCGGG               C^[CT]CG[GA]G
BspEI        2780  TCCGGA               T^CCGGA
BspHI        1811  TCATGA               T^CATGA
BstEII        865  GGTTACC              G^GT[ACGT]ACC
BstPI         865  GGTTACC              G^GT[ACGT]ACC
BstXI        1794  CCAACCTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1684  CCTCAGG              CC^T[ACGT]AGG
DrdI          764  GACCTCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1450  CGGCCA               [CT]^GGCC[GA]
Eam1105I     1216  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1684  CCTCAGG              CC^T[ACGT]AGG
EcoNI         210  CCTCCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       865  GGTTACC              G^GT[ACGT]ACC
EcoRV        1807  GATATC               GAT^ATC
FbaI         2076  TGATCA               T^GATCA
KasI         2957  GGCGCC               G^GCGCC
MamI         1595  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2780  TCCGGA               T^CCGGA
MslI         1812  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         2957  GGCGCC               GG^CGCC
NdeI         1864  CATATG               CA^TATG
PflFI        3241  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2119  CCAGATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1811  TCATGA               T^CATGA
SexAI        1200  ACCAGGT              A^CC[AT]GGT
SfoI         2957  GGCGCC               GGC^GCC
SmaI         2615  CCCGGG               CCC^GGG
SmlI         3637  CTTGAG               C^T[CT][GA]AG
Tsp45I        494  GTGAC                ^GT[GC]AC
Tth111I      3241  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2119  CCAGATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2888  CCACGCTGTCGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2615  CCCGGG               C^CCGGG
XmaI         2615  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I        243  GGTACC               G^GTACC
             3052  GGTACC              
AcyI          818  GACGTC               G[GA]^CG[CT]C
             2957  GGCGCC              
Asp718I       243  GGTACC               G^GTACC
             3052  GGTACC              
BcnI         2615  CCCGG                CC^[GC]GG
             2616  CCGGG               
BsaHI         818  GACGTC               G[GA]^CG[CT]C
             2957  GGCGCC              
BsaI          600  GAGACC               GGTCTC(1/5)
             1522  GGTCTC              
BsgI         3172  CTGCAC               GTGCAG(16/14)
             3835  GTGCAG              
BsmI          979  GCATTC               GAATGC(1/-1)
             3162  GCATTC              
BspMI        1934  ACCTGC               ACCTGC(4/8)
             3170  ACCTGC              
BsrDI          95  CATTGC               GCAATG(2/0)
             1657  GCAATG              
BsrFI        2654  ACCGGC               [GA]^CCGG[CT]
             3389  ACCGGC              
BstAPI       1929  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3681  GCAGCAGCTGC         
Cfr10I       2654  ACCGGC               [GA]^CCGG[CT]
             3389  ACCGGC              
EcoRI        3189  GAATTC               G^AATTC
             3330  GAATTC              
HaeII        2957  GGCGCC               [GA]GCGC^[CT]
             3641  AGCGCC              
Hin1I         818  GACGTC               G[GA]^CG[CT]C
             2957  GGCGCC              
HindIII      1788  AAGCTT               A^AGCTT
             2827  AAGCTT              
KpnI          243  GGTACC               GGTAC^C
             3052  GGTACC              
NciI         2615  CCCGG                CC^[GC]GG
             2616  CCGGG               
NspI          554  GCATGT               [GA]CATG^[CT]
             2872  GCATGT              
PpuMI        1701  AGGTCCC              [GA]G^G[AT]CC[CT]
             3321  GGGACCT             
PstI          791  CTGCAG               CTGCA^G
             1936  CTGCAG              
PvuII        2812  CAGCTG               CAG^CTG
             3685  CAGCTG              
SacI           47  GAGCTC               GAGCT^C
             3146  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanI        BanII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NsiI        PleI        RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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