Restriction map for pF1KSDA0587
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
Asp700I     Asp718I     AspEI       AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BglI        BlnI        
BlpI        Bpu1102I    BsaWI       BseAI       BseRI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FseI        FspI        HindIII     
KasI        KpnI        KspI        MluI        MroI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII           5  CGCG                 CG^CG
AcyI           70  GGCGTC               G[GA]^CG[CT]C
AflII        2614  CTTAAG               C^TTAAG
AflIII       1269  ACATGT               A^C[GA][CT]GT
Alw44I        719  GTGCAC               G^TGCAC
AlwNI          31  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         719  GTGCAC               G^TGCAC
AspI         2719  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         830  GGATCC               G^GATCC
BclI         1092  TGATCA               T^GATCA
BcnI           66  CCGGG                CC^[GC]GG
BfrI         2614  CTTAAG               C^TTAAG
BsaAI        1306  TACGTG               [CT]AC^GT[GA]
BsaBI        2705  GATTATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          70  GGCGTC               G[GA]^CG[CT]C
BsaI          134  GAGACC               GGTCTC(1/5)
BsiEI          63  CGACCG               CG[GA][CT]^CG
BsmFI        1112  GTCCC                GGGAC(10/14)
BsmI          994  GAATGC               GAATGC(1/-1)
Bsp1407I     3291  TGTACA               T^GTACA
BspLU11I     1269  ACATGT               A^CATGT
BsrGI        3291  TGTACA               T^GTACA
BstAPI       3361  GCATACCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI           5  CGCG                 CG^CG
DraII        1110  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DrdI           64  GACCGGGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          825  CCATGG               C^C[GA][CT]GG
EcoO109I     1110  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      3359  ATGCAT               ATGCA^T
FbaI         1092  TGATCA               T^GATCA
HaeII        3195  GGCGCT               [GA]GCGC^[CT]
Hin1I          70  GGCGTC               G[GA]^CG[CT]C
MamI         2705  GATTATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          334  CATGTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI            5  CGCG                 CG^CG
NciI           66  CCGGG                CC^[GC]GG
NcoI          825  CCATGG               C^CATGG
NdeI         2552  CATATG               CA^TATG
NsiI         3359  ATGCAT               ATGCA^T
PflFI        2719  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         544  CCACGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          516  GACTC                GAGTC(4/5)
PpuMI        1110  AGGTCCC              [GA]G^G[AT]CC[CT]
SexAI        1995  ACCAGGT              A^CC[AT]GGT
SmlI         2614  CTTAAG               C^T[CT][GA]AG
SpeI         1507  ACTAGT               A^CTAGT
SphI           77  GCATGC               GCATG^C
SspBI        3291  TGTACA               T^GTACA
Tth111I      2719  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        544  CCACGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         3212  GTCTAC               GT^[AC][GT]AC
             3373  GTCTAC              
AseI          979  ATTAAT               AT^TAAT
             1239  ATTAAT              
AsnI          979  ATTAAT               AT^TAAT
             1239  ATTAAT              
AspHI         719  GTGCAC               G[AT]GC[AT]^C
             1265  GAGCAC              
BglII        1057  AGATCT               A^GATCT
             1629  AGATCT              
BmyI          719  GTGCAC               G[AGT]GC[ACT]^C
             1265  GAGCAC              
BpmI         2266  CTCCAG               CTGGAG(16/14)
             3207  CTCCAG              
BsiHKAI       719  GTGCAC               G[AT]GC[AT]^C
             1265  GAGCAC              
Bsp1286I      719  GTGCAC               G[AGT]GC[ACT]^C
             1265  GAGCAC              
BssSI        2129  CACGAG               CACGAG(-5/-1)
             1351  CTCGTG              
CfoI            6  GCGC                 GCG^C
             3196  GCGC                
DraI          582  TTTAAA               TTT^AAA
             2698  TTTAAA              
FauI           86  CCCGC                CCCGC(4/6)
              224  CCCGC               
HapII          66  CCGG                 C^CGG
             2912  CCGG                
HhaI            6  GCGC                 GCG^C
             3196  GCGC                
HinP1I          6  GCGC                 G^CGC
             3196  GCGC                
HpaI         1408  GTTAAC               GTT^AAC
             2646  GTTAAC              
HpaII          66  CCGG                 C^CGG
             2912  CCGG                
HphI           50  TCACC                GGTGA(8/7)
               83  TCACC               
MspA1I        677  CAGCTG               C[AC]G^C[GT]G
             2906  CAGCTG              
MspI           66  CCGG                 C^CGG
             2912  CCGG                
PshBI         979  ATTAAT               AT^TAAT
             1239  ATTAAT              
PvuII         677  CAGCTG               CAG^CTG
             2906  CAGCTG              
SapI         1774  GAAGAGC              GCTCTTC(1/4)
              874  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BalI        BbsI        BbvI        
BfaI        BpuAI       BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HgaI        
HincII      HindII      HinfI       ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MflI        MluNI       MnlI        MscI        
MseI        MunI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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