Restriction map for pF1KSDA0587
 (Linear) (Six-base) MAPSORT of: KIAA0587.seq  Check: 4801  from: 1  to: 3402



KIAA0587.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0    3213    3374    3402 
   Size:       3213     161      28

  Fragments arranged by size: 

                3213     161      28
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1397    2210    3402 
   Size:       1087     310     813    1192

  Fragments arranged by size: 

                1192    1087     813     310
 
AflII C'TTAA_G

Cuts at:      0    2614    3402 
   Size:       2614     788
 
AflIII A'CryG_T

Cuts at:      0    1269    3402 
   Size:       1269    2133
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36    3402 
   Size:         36    3366
 
ApaLI G'TGCA_C

Cuts at:      0     719    3402 
   Size:        719    2683
 
ApoI r'AATT_y

Cuts at:      0     190     205     322     601    3187    3402 
   Size:        190      15     117     279    2586     215

  Fragments arranged by size: 

                2586     279     215     190     117      15
 
AseI AT'TA_AT

Cuts at:      0     980    1240    3402 
   Size:        980     260    2162

  Fragments arranged by size: 

                2162     980     260
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     442     475    3402 
   Size:        442      33    2927

  Fragments arranged by size: 

                2927     442      33
 
BamHI G'GATC_C

Cuts at:      0     830    3402 
   Size:        830    2572
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     366     947    3179    3402 
   Size:        366     581    2232     223

  Fragments arranged by size: 

                2232     581     366     223
 
BclI T'GATC_A

Cuts at:      0    1092    3402 
   Size:       1092    2310
 
BfrBI ATG'CAT

Cuts at:      0    3361    3402 
   Size:       3361      41
 
BglII A'GATC_T

Cuts at:      0    1057    1629    3402 
   Size:       1057     572    1773

  Fragments arranged by size: 

                1773    1057     572
 
Bme1580I G_kGCm'C

Cuts at:      0     723    3402 
   Size:        723    2679
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      17     693    3402 
   Size:         17     676    2709

  Fragments arranged by size: 

                2709     676      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1713    3402 
   Size:       1713    1689
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2251    3192    3402 
   Size:       2251     941     210

  Fragments arranged by size: 

                2251     941     210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     128    3402 
   Size:        128    3274
 
BsaAI yAC'GTr

Cuts at:      0    1308    3402 
   Size:       1308    2094
 
BsaBI GATnn'nnATC

Cuts at:      0    2709    3402 
   Size:       2709     693
 
BsaHI Gr'CG_yC

Cuts at:      0      71    3402 
   Size:         71    3331
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1046    1226    1330    1375    2042    2308    2675    3402 
   Size:       1046     180     104      45     667     266     367     727

  Fragments arranged by size: 

                1046     727     667     367     266     180     104      45
            
 
BsiEI CG_ry'CG

Cuts at:      0      66    3402 
   Size:         66    3336
 
BsiHKAI G_wGCw'C

Cuts at:      0     723    1269    3402 
   Size:        723     546    2133

  Fragments arranged by size: 

                2133     723     546
 
BsmI GAATG_Cn'

Cuts at:      0    1000    3402 
   Size:       1000    2402
 
Bsp1286I G_dGCh'C

Cuts at:      0     723    1269    3402 
   Size:        723     546    2133

  Fragments arranged by size: 

                2133     723     546
 
BsrDI GCAATG_nn'

Cuts at:      0     794    1229    2427    2453    2888    3090    3105    3402 
   Size:        794     435    1198      26     435     202      15     297

  Fragments arranged by size: 

                1198     794     435     435     297     202      26      15
            
 
BsrGI T'GTAC_A

Cuts at:      0    3291    3402 
   Size:       3291     111
 
BssSI C'ACGA_G

Cuts at:      0    1351    2129    3402 
   Size:       1351     778    1273

  Fragments arranged by size: 

                1351    1273     778
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3367    3402 
   Size:       3367      35
 
BstYI r'GATC_y

Cuts at:      0     830    1057    1629    3318    3402 
   Size:        830     227     572    1689      84

  Fragments arranged by size: 

                1689     830     572     227      84
 
BtgI C'CryG_G

Cuts at:      0     825    3402 
   Size:        825    2577
 
BtsI GCAGTG_nn'

Cuts at:      0    1943    2054    3402 
   Size:       1943     111    1348

  Fragments arranged by size: 

                1943    1348     111
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2084    2119    3402 
   Size:       2084      35    1283

  Fragments arranged by size: 

                2084    1283      35
 
DraI TTT'AAA

Cuts at:      0     584    2700    3402 
   Size:        584    2116     702

  Fragments arranged by size: 

                2116     702     584
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      70    3402 
   Size:         70    3332
 
EaeI y'GGCC_r

Cuts at:      0     552    1835    2424    3047    3113    3225    3402 
   Size:        552    1283     589     623      66     112     177

  Fragments arranged by size: 

                1283     623     589     552     177     112      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     650     881    1195    1769    2725    2984    3402 
   Size:        650     231     314     574     956     259     418

  Fragments arranged by size: 

                 956     650     574     418     314     259     231
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55    3402 
   Size:         55    3347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1397    2210    2251    3192    3402 
   Size:       1087     310     813      41     941     210

  Fragments arranged by size: 

                1087     941     813     310     210      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1111    3402 
   Size:       1111    2291
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     432     905    2803    3196    3402 
   Size:        432     473    1898     393     206

  Fragments arranged by size: 

                1898     473     432     393     206
 
HaeII r_GCGC'y

Cuts at:      0    3199    3402 
   Size:       3199     203
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2133    2165    2785    2817    2887    2919    3402 
   Size:       2133      32     620      32      70      32     483

  Fragments arranged by size: 

                2133     620     483      70      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1410    2648    2718    3402 
   Size:       1410    1238      70     684

  Fragments arranged by size: 

                1410    1238     684      70
 
HpaI GTT'AAC

Cuts at:      0    1410    2648    3402 
   Size:       1410    1238     754

  Fragments arranged by size: 

                1410    1238     754
 
MfeI C'AATT_G

Cuts at:      0    1715    2174    2831    3402 
   Size:       1715     459     657     571

  Fragments arranged by size: 

                1715     657     571     459
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    3402 
   Size:        776    2626
 
MscI TGG'CCA

Cuts at:      0     554    1837    2426    3049    3115    3227    3402 
   Size:        554    1283     589     623      66     112     175

  Fragments arranged by size: 

                1283     623     589     554     175     112      66
 
MslI CAynn'nnrTG

Cuts at:      0     338    3402 
   Size:        338    3064
 
MspA1I CmG'CkG

Cuts at:      0     679    2908    3402 
   Size:        679    2229     494

  Fragments arranged by size: 

                2229     679     494
 
NcoI C'CATG_G

Cuts at:      0     825    3402 
   Size:        825    2577
 
NdeI CA'TA_TG

Cuts at:      0    2553    3402 
   Size:       2553     849
 
NsiI A_TGCA'T

Cuts at:      0    3363    3402 
   Size:       3363      39
 
NspI r_CATG'y

Cuts at:      0      81     131    1021    1194    1273    3402 
   Size:         81      50     890     173      79    2129

  Fragments arranged by size: 

                2129     890     173      81      79      50
 
PciI A'CATG_T

Cuts at:      0    1269    3402 
   Size:       1269    2133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     550    3402 
   Size:        550    2852
 
PpuMI rG'GwC_Cy

Cuts at:      0    1111    3402 
   Size:       1111    2291
 
PsiI TTA'TAA

Cuts at:      0     430    3402 
   Size:        430    2972
 
PstI C_TGCA'G

Cuts at:      0      20     939    3141    3402 
   Size:         20     919    2202     261

  Fragments arranged by size: 

                2202     919     261      20
 
PvuII CAG'CTG

Cuts at:      0     679    2908    3402 
   Size:        679    2229     494

  Fragments arranged by size: 

                2229     679     494
 
SapI GCTCTTCn'nnn_

Cuts at:      0     881    1769    3402 
   Size:        881     888    1633

  Fragments arranged by size: 

                1633     888     881
 
SexAI A'CCwGG_T

Cuts at:      0    1995    3402 
   Size:       1995    1407
 
SfcI C'TryA_G

Cuts at:      0      16     215     253     848     935    2471    3080    3137 
   Size:         16     199      38     595      87    1536     609      57

Cuts at:   3137    3402 
   Size:        265

  Fragments arranged by size: 

                1536     609     595     265     199      87      57      38
                  16
 
SmlI C'TyrA_G

Cuts at:      0    2614    3402 
   Size:       2614     788
 
SpeI A'CTAG_T

Cuts at:      0    1507    3402 
   Size:       1507    1895
 
SphI G_CATG'C

Cuts at:      0      81    3402 
   Size:         81    3321
 
SspI AAT'ATT

Cuts at:      0    1244    2137    2506    3402 
   Size:       1244     893     369     896

  Fragments arranged by size: 

                1244     896     893     369
 
StyI C'CwwG_G

Cuts at:      0     140     549     825    1115    1601    2857    3402 
   Size:        140     409     276     290     486    1256     545

  Fragments arranged by size: 

                1256     545     486     409     290     276     140
 
TatI w'GTAC_w

Cuts at:      0     727    3068    3291    3402 
   Size:        727    2341     223     111

  Fragments arranged by size: 

                2341     727     223     111
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     114     146    3402 
   Size:        114      32    3256

  Fragments arranged by size: 

                3256     114      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2722    3402 
   Size:       2722     680
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII    AlwNI    ApaLI     ApoI     AseI
     BaeI    BamHI     BbsI     BclI    BfrBI    BglII Bme1580I     BmrI
     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI    BssSI   BstAPI    BstYI
     BtgI     BtsI    CspCI     DraI     DrdI     EaeI     EarI     EciI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     HpaI     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PciI    PflMI    PpuMI     PsiI     PstI    PvuII     SapI    SexAI
     SfcI     SmlI     SpeI     SphI     SspI     StyI     TatI     TstI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI
     BmtI   Bpu10I    BpuEI    BsaWI    BsaXI    BseRI    BseYI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     KasI     KpnI     MluI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SrfI     StuI     SwaI    TaqII     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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