Restriction map for pF1KSDA0307
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AvaI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BclI        
BfaI        BfrI        BlnI        BsaAI       BsaHI       
BsaI        BsiEI       BsiWI       BsoBI       BsrDI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
CpoI        DraI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRV       
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MluI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII        109  TCCGGA               T^CCGGA
AflIII       1643  ACATGT               A^C[GA][CT]GT
AhdI         1689  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1093  CCTGAGG              CC^T[ACGT]AGG
AspEI        1689  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        1017  GGATCC               G^GATCC
BanI          963  GGTGCC               G^G[CT][GA]CC
BcgI         2076  CGAAGACTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         817  GGATAC               GTATCC(6/5)
BglI           40  GCCCCCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1526  AGATCT               A^GATCT
BlpI         1499  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1499  GCTCAGC              GC^T[ACGT]AGC
BseAI         109  TCCGGA               T^CCGGA
BseRI        1830  CTCCTC               GAGGAG(10/8)
BsgI         1263  CTGCAC               GTGCAG(16/14)
BsmBI         294  CGTCTC               CGTCTC(1/5)
BsmI         1845  GAATGC               GAATGC(1/-1)
Bsp1407I      811  TGTACA               T^GTACA
BspDI        1169  ATCGAT               AT^CGAT
BspEI         109  TCCGGA               T^CCGGA
BspHI        1386  TCATGA               T^CATGA
BspLU11I     1643  ACATGT               A^CATGT
BspMI        1128  GCAGGT               ACCTGC(4/8)
BsrBI        1971  GAGCGG               CCGCTC(-3/-3)
BsrFI        1378  GCCGGT               [GA]^CCGG[CT]
BsrGI         811  TGTACA               T^GTACA
BssSI         898  CTCGTG               CACGAG(-5/-1)
BstEII        921  GGTGACC              G^GT[ACGT]ACC
BstPI         921  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1093  CCTGAGG              CC^T[ACGT]AGG
CelII        1499  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1378  GCCGGT               [GA]^CCGG[CT]
ClaI         1169  ATCGAT               AT^CGAT
Eam1105I     1689  GACAGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1093  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       921  GGTGACC              G^GT[ACGT]ACC
EcoRI        1081  GAATTC               G^AATTC
EcoT22I       939  ATGCAT               ATGCA^T
HgaI          865  GACGC                GACGC(5/10)
MfeI          905  CAATTG               C^AATTG
MroI          109  TCCGGA               T^CCGGA
MseI         1136  TTAA                 T^TAA
MspA1I        401  CAGCTG               C[AC]G^C[GT]G
MunI          905  CAATTG               C^AATTG
NsiI          939  ATGCAT               ATGCA^T
PflMI        1125  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1355  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         401  CAGCTG               CAG^CTG
RcaI         1386  TCATGA               T^CATGA
SapI         1580  GCTCTTC              GCTCTTC(1/4)
SspBI         811  TGTACA               T^GTACA
SspI          890  AATATT               AAT^ATT
Tru9I        1136  TTAA                 T^TAA
Van91I       1125  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          278  CCATCCTCCGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         345  CGCG                 CG^CG
              665  CGCG                
AcsI          151  AAATTT               [GA]^AATT[CT]
             1081  GAATTC              
Alw44I        564  GTGCAC               G^TGCAC
             1315  GTGCAC              
AlwNI         373  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1502  CAGCCTCTG           
ApaLI         564  GTGCAC               G^TGCAC
             1315  GTGCAC              
ApoI          151  AAATTT               [GA]^AATT[CT]
             1081  GAATTC              
Asp700I       747  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1569  GAAGGCCTTC          
BalI          832  TGGCCA               TGG^CCA
             1962  TGGCCA              
BsaBI        1197  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1331  GATTGTCATC          
BsaWI          55  ACCGGA               [AT]^CCGG[AT]
              109  TCCGGA              
BstUI         345  CGCG                 CG^CG
              665  CGCG                
DraII         141  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1355  AGGTCCC             
EcoO109I      141  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1355  AGGTCCC             
FauI           73  GCGGG                CCCGC(4/6)
             1904  GCGGG               
MamI         1197  GATGTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1331  GATTGTCATC          
MluNI         832  TGGCCA               TGG^CCA
             1962  TGGCCA              
MscI          832  TGGCCA               TGG^CCA
             1962  TGGCCA              
MvnI          345  CGCG                 CG^CG
              665  CGCG                
NcoI           44  CCATGG               C^CATGG
             1959  CCATGG              
PstI          681  CTGCAG               CTGCA^G
             1508  CTGCAG              
SacI          220  GAGCTC               GAGCT^C
             1497  GAGCTC              
StuI          827  AGGCCT               AGG^CCT
             1571  AGGCCT              
XmnI          747  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1569  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AspI        AvaII       BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XhoII       
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