Restriction map for pF1KSDA0307
 (Linear) (Six-base) MAPSORT of: KIAA0307.seq  Check: 8504  from: 1  to: 2118



KIAA0307.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1119    2118 
   Size:       1119     999
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     625     878    1273    1560    1568    2030    2118 
   Size:        400     225     253     395     287       8     462      88

  Fragments arranged by size: 

                 462     400     395     287     253     225      88       8
            
 
AflIII A'CryG_T

Cuts at:      0    1643    2118 
   Size:       1643     475
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1694    2118 
   Size:       1694     424
 
AleI CACnn'nnGTG

Cuts at:      0    1012    2118 
   Size:       1012    1106
 
AlwNI CAG_nnn'CTG

Cuts at:      0     378    1507    2118 
   Size:        378    1129     611

  Fragments arranged by size: 

                1129     611     378
 
ApaLI G'TGCA_C

Cuts at:      0     564    1315    2118 
   Size:        564     751     803

  Fragments arranged by size: 

                 803     751     564
 
ApoI r'AATT_y

Cuts at:      0     151    1081    2118 
   Size:        151     930    1037

  Fragments arranged by size: 

                1037     930     151
 
BamHI G'GATC_C

Cuts at:      0    1017    2118 
   Size:       1017    1101
 
BanI G'GyrC_C

Cuts at:      0     963    2118 
   Size:        963    1155
 
BanII G_rGCy'C

Cuts at:      0     224     664    1501    2118 
   Size:        224     440     837     617

  Fragments arranged by size: 

                 837     617     440     224
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     613     869    2084    2118 
   Size:        613     256    1215      34

  Fragments arranged by size: 

                1215     613     256      34
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2065    2099    2118 
   Size:       2065      34      19

  Fragments arranged by size: 

                2065      34      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     811    2118 
   Size:        811    1307
 
BfrBI ATG'CAT

Cuts at:      0     941    2118 
   Size:        941    1177
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46    2118 
   Size:         46    2072
 
BglII A'GATC_T

Cuts at:      0    1526    2118 
   Size:       1526     592
 
BlpI GC'TnA_GC

Cuts at:      0    1500    2118 
   Size:       1500     618
 
Bme1580I G_kGCm'C

Cuts at:      0     568     968    1319    2118 
   Size:        568     400     351     799

  Fragments arranged by size: 

                 799     568     400     351
 
BmgBI CAC'GTC

Cuts at:      0     540    2118 
   Size:        540    1578
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     140     483     620    1994    2118 
   Size:        140     343     137    1374     124

  Fragments arranged by size: 

                1374     343     140     137     124
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2001    2118 
   Size:       2001     117
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    1622    1800    1839    1859    2118 
   Size:        901     721     178      39      20     259

  Fragments arranged by size: 

                 901     721     259     178      39      20
 
BsaBI GATnn'nnATC

Cuts at:      0    1201    1335    2118 
   Size:       1201     134     783

  Fragments arranged by size: 

                1201     783     134
 
BsaWI w'CCGG_w

Cuts at:      0      55     109    2118 
   Size:         55      54    2009

  Fragments arranged by size: 

                2009      55      54
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     915     945    1183    1213    2118 
   Size:        915      30     238      30     905

  Fragments arranged by size: 

                 915     905     238      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1821    2118 
   Size:       1821     297
 
BseYI C'CCAG_C

Cuts at:      0    1520    1821    2118 
   Size:       1520     301     297

  Fragments arranged by size: 

                1520     301     297
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1248    2118 
   Size:       1248     870
 
BsiHKAI G_wGCw'C

Cuts at:      0     224     511     568    1319    1501    2118 
   Size:        224     287      57     751     182     617

  Fragments arranged by size: 

                 751     617     287     224     182      57
 
BsmI GAATG_Cn'

Cuts at:      0    1851    2118 
   Size:       1851     267
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     300    2118 
   Size:        300    1818
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     511     568     664     968    1319    1501    2118 
   Size:        224     287      57      96     304     351     182     617

  Fragments arranged by size: 

                 617     351     304     287     224     182      96      57
            
 
BspEI T'CCGG_A

Cuts at:      0     109    2118 
   Size:        109    2009
 
BspHI T'CATG_A

Cuts at:      0    1386    2118 
   Size:       1386     732
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1119    2118 
   Size:       1119     999
 
BsrBI CCG'CTC

Cuts at:      0    1973    2118 
   Size:       1973     145
 
BsrFI r'CCGG_y

Cuts at:      0    1378    2118 
   Size:       1378     740
 
BsrGI T'GTAC_A

Cuts at:      0     811    2118 
   Size:        811    1307
 
BssSI C'ACGA_G

Cuts at:      0     898    2118 
   Size:        898    1220
 
BstEII G'GTnAC_C

Cuts at:      0     921    2118 
   Size:        921    1197
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1327    1960    2059    2118 
   Size:       1327     633      99      59

  Fragments arranged by size: 

                1327     633      99      59
 
BstYI r'GATC_y

Cuts at:      0      19     593    1017    1056    1440    1526    2043    2118 
   Size:         19     574     424      39     384      86     517      75

  Fragments arranged by size: 

                 574     517     424     384      86      75      39      19
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1094    2118 
   Size:       1094    1024
 
BtgI C'CryG_G

Cuts at:      0      44      90    1403    1631    1959    2118 
   Size:         44      46    1313     228     328     159

  Fragments arranged by size: 

                1313     328     228     159      46      44
 
BtsI GCAGTG_nn'

Cuts at:      0    1948    2118 
   Size:       1948     170
 
ClaI AT'CG_AT

Cuts at:      0    1170    2118 
   Size:       1170     948
 
EaeI y'GGCC_r

Cuts at:      0      50     290     832    1962    2118 
   Size:         50     240     542    1130     156

  Fragments arranged by size: 

                1130     542     240     156      50
 
EarI CTCTTCn'nnn_

Cuts at:      0    1587    1629    1803    2118 
   Size:       1587      42     174     315

  Fragments arranged by size: 

                1587     315     174      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1429    1691    2118 
   Size:       1429     262     427

  Fragments arranged by size: 

                1429     427     262
 
EcoICRI GAG'CTC

Cuts at:      0     222    1499    2118 
   Size:        222    1277     619

  Fragments arranged by size: 

                1277     619     222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400     625     878     901    1273    1560    1568    1622 
   Size:        400     225     253      23     372     287       8      54

Cuts at:   1622    1800    1839    1859    2030    2118 
   Size:        178      39      20     171      88

  Fragments arranged by size: 

                 400     372     287     253     225     178     171      88
                  54      39      23      20       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     142    1356    2118 
   Size:        142    1214     762

  Fragments arranged by size: 

                1214     762     142
 
EcoRI G'AATT_C

Cuts at:      0    1081    2118 
   Size:       1081    1037
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     375    2118 
   Size:        375    1743
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     915     947     964     996    1318    1350    1603    1635 
   Size:        915      32      17      32     322      32     253      32

Cuts at:   1635    1680    1706    1712    1738    2118 
   Size:         45      26       6      26     380

  Fragments arranged by size: 

                 915     380     322     253      45      32      32      32
                  32      26      26      17       6
 
MfeI C'AATT_G

Cuts at:      0     905    2118 
   Size:        905    1213
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     251    2071    2118 
   Size:        251    1820      47

  Fragments arranged by size: 

                1820     251      47
 
MscI TGG'CCA

Cuts at:      0     834    1964    2118 
   Size:        834    1130     154

  Fragments arranged by size: 

                1130     834     154
 
MslI CAynn'nnrTG

Cuts at:      0     533     649     946    1012    1112    2118 
   Size:        533     116     297      66     100    1006

  Fragments arranged by size: 

                1006     533     297     116     100      66
 
MspA1I CmG'CkG

Cuts at:      0     403    2118 
   Size:        403    1715
 
NcoI C'CATG_G

Cuts at:      0      44    1959    2118 
   Size:         44    1915     159

  Fragments arranged by size: 

                1915     159      44
 
NsiI A_TGCA'T

Cuts at:      0     943    2118 
   Size:        943    1175
 
NspI r_CATG'y

Cuts at:      0     245    1647    2028    2100    2118 
   Size:        245    1402     381      72      18

  Fragments arranged by size: 

                1402     381     245      72      18
 
PasI CC'CwG_GG

Cuts at:      0    2052    2118 
   Size:       2052      66
 
PciI A'CATG_T

Cuts at:      0    1643    2118 
   Size:       1643     475
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1131    2118 
   Size:       1131     987
 
PfoI T'CCnGG_A

Cuts at:      0    1564    1710    2018    2118 
   Size:       1564     146     308     100

  Fragments arranged by size: 

                1564     308     146     100
 
PpuMI rG'GwC_Cy

Cuts at:      0    1356    2118 
   Size:       1356     762
 
PstI C_TGCA'G

Cuts at:      0     685    1512    2118 
   Size:        685     827     606

  Fragments arranged by size: 

                 827     685     606
 
PvuII CAG'CTG

Cuts at:      0     403    2118 
   Size:        403    1715
 
SacI G_AGCT'C

Cuts at:      0     224    1501    2118 
   Size:        224    1277     617

  Fragments arranged by size: 

                1277     617     224
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1587    2118 
   Size:       1587     531
 
SfcI C'TryA_G

Cuts at:      0     681    1508    1803    2118 
   Size:        681     827     295     315

  Fragments arranged by size: 

                 827     681     315     295
 
SspI AAT'ATT

Cuts at:      0     892    2118 
   Size:        892    1226
 
StuI AGG'CCT

Cuts at:      0     829    1573    2118 
   Size:        829     744     545

  Fragments arranged by size: 

                 829     744     545
 
StyI C'CwwG_G

Cuts at:      0      44     765    1536    1914    1959    2118 
   Size:         44     721     771     378      45     159

  Fragments arranged by size: 

                 771     721     378     159      45      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1149    2118 
   Size:       1149     969
 
TatI w'GTAC_w

Cuts at:      0     209     811    1253    2118 
   Size:        209     602     442     865

  Fragments arranged by size: 

                 865     602     442     209
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     292     324     678     710    2118 
   Size:        292      32     354      32    1408

  Fragments arranged by size: 

                1408     354     292      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     232     463    1746    2118 
   Size:        232     231    1283     372

  Fragments arranged by size: 

                1283     372     232     231
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285    2118 
   Size:        285    1833
 
XmnI GAAnn'nnTTC

Cuts at:      0     751    1573    2118 
   Size:        751     822     545

  Fragments arranged by size: 

                 822     751     545
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI    AlwNI    ApaLI     ApoI
    BamHI     BanI    BanII     BbsI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
     MfeI     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PasI     PciI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
     SapI     SfcI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI     BclI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsiEI    BsiWI    BsrDI   BssHII   BstAPI    BstBI  BstZ17I
    BtgZI    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI     XbaI     XhoI     XmaI
     ZraI
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